A high incidence of esophageal cancer exists in China. Surgical resection remains the dominant therapeutic intervention for patients with operable esophageal carcinoma. However, alternative strategies are actively applied to reduce the frequency of post-op-erative local or distant disease recurrence and to prolong survival. These strategies include neoadjuvant and adjuvant therapies. This review discusses the current knowledge, as well as available data and information, with regard to neoadjuvant and adjuvant therapies for patients with locally advanced esophageal cancer.

Objective To compare the influence of general anesthesia and spinal anesthesia on the mortality in rats with fat embolism.Methods Three hundred and seventy five rats were randomly assigned to receive general anesthesia （group GF,n=125）,spinal anesthesia （group SF,n=125）,or no anesthesia （group F,n=125）.Rats were injected with a half lethal dose of fat from caudal vein 20 min after anesthesia induction. The mortality was recorded at 2,8,12,and 24 h after fat injection,respectively.Results The mortality was lower in group GF than those in groups SF and F （P0.05）,while there was no statistical difference between group SF and group F.Conclusion The mortality in rats with fat embolism under general anesthesia was lower than that under spinal anesthesia.

Objective To investigate the protective immunity of the vaccine against schistosomiasis,a mutant of Mr 23 000 membrane protein DNA(Sj23DNA) without the homologous sequence of ME491.Methods The mutant of Sj23 DNA with no homologous sequence of ME491 on the cell membrane of human melanoma was obtained by overlap PCR.The mutant was transfected into human embryonic kidney cells of the line HEK293.Indirect fluorescent antibody test(IFAT) was used to detect the expressed protein.Expression of the mutant of Sj23DNA in muscular cells of mice was conducted through vaccinating the mouse with 100 ?g purified plasmids by injecting them into the quadriceps muscle of thigh.Four weeks after the immunization,the quadriceps muscles were taken and cryostat sections were prepared for detecting the expression by IFAT.Forty BALB/c mice were randomly divided into four groups and injected with the mutant of pcDNA3-Sj23 plasmid DNA,pcDNA3-Sj23 plasmid DNA,pcDNA3 blank plasmid(100 ?g per mouse) and sterile saline(30 ?l per mouse) respectively.Four weeks after the immunization,mice were challenged with cercariae(40?2 cercariae per mouse) by abdominal skin penetration.Mice were then killed 6 weeks later,perfusion and squash methods were carried out to collect the adult worms and the number of eggs per gram of liver tissue was calculated.Worm and egg reduction rates were used to evaluate the protective immunity.Results Specific fluorescence was demonstrated in muscular cells of mice vaccinated with the mutant of pcDNA3-Sj23.The worm reduction rate and egg reduction rate were 40.3% and 42.8% respectively in the mutant of pcDNA3-Sj23 group,which were higher than those in the pcDNA3-Sj23 plasmid group(33.1% and 28.9% respectively).The difference between these two groups was significant(P

Objective To encode the protein cystatin M with CST6 gene and construct a CST6-overexpression vector,and transfect it into the gastric cancer cells SGC-7901 in order to observe the effect of cystatin M on the proliferation and migration abilities of SGC-7901 cells.Methods There were three groups in the experiment:pcDNA3.1(+)-CST6 group,pcDNA3.1 (+)group and non-transfected group.RT-PCR and Western blot were used to identify the RNA expression of CST6 in SGC-7901/CST6 cells.The proliferation and migration abilities of the transfected cells were detected by MTT and cell wound healing assay,respectively.Results SGC-7901/CST6 cells stably expressed CST6 gene RNA and cystatin M protein.The proliferation and migration of pcDNA3 .1 (+)-CST6 group cells were reduced compared with those of cells in the other two groups (P<0.05).Conclusion Cystatin M can inhibit the proliferation and migration of SGC-7901 cells.

This paper reviews the general situation, prevention status, guidelines and specifications, system construction, measures application of venous thromboembolism (VTE) . At present, there are many mature guidelines and standards, and the latest version of the guidelines has been issued in China as a scientific and objective reference basis and theoretical source for clinical prevention. Many hospitals have set up in-house VTE prevention and treatment systems. However, there are still many obstacles in the clinical implementation process. We can develop VTE related clinical decision-making support system and make VTE prevention and decision standardized and scientific with the help of intelligent big data platforms, so as to reduce the occurrence of VTE.

Objective To investigate the effects of inhibiting miR-29 on growth,invasion and metastasis of pancreatic cancer PANC1 cells,and explore the potential mechanism.Methods Oligonucleotides inhibiting miR-29 (anti miR-29) and control oligonucleotides (miR NC) were used to transfect PANC1 cells to establish anti miR-29 PANC1 cells and miR NC PANC1 cells.Transient transfection of PUMA siRNA,E-cadherin siRNA or NC siRNA was used to construct cotransfected anti miR29 + PUMA-siRNA-PANC1 cells and anti-miR-29 + E-cadherin-siRNA-PANC1 cells.Number of colony formations was observed,cell survival was detected by MTT,cell apoptosis was measured by flow cytometry,cell invasion was detected by transwell chamber assay,and cell migration was detected by wound healing assay.Subcutaneous injection of anti miR-29 PANC1 cells was used to establish xenograft nude mice model,and venous injection of anti miR-29 PANC1 cells was used to establish lung metastasis nude mice model,and the subcutaneous and venous injection of PANC1 cells served as control.The growth of xenograft and the number of lung metastatic nodules were observed.TUNEL method was used to detect cell apoptosis in xenograft and immunohistochemical analysis was used to detect PUMA and E-cadherin in xenograft.Results The survival rate of PANC1,miR-NC-PANC1 and anti-miR-29-PANC1 cells was 100％,(96.8 ± 2.8) ％ and (24.4 ± 3.2) ％.The number of colony formation was (213 ± 36),(196 ± 28) and (37 ± 6) per 100 high power field.The number of transmembrane cells was (56.3 ± 9.6),(49.8-± 7.3) and (11.2 ± 3.4) per 400 high power field.The distance of cell migration was (260 ± 48),(247 ± 46) and (53 ± 7) μm.Cell apoptosis rate was (1.5 +0.9) ％,(2.6 + 0.9) ％ and (22.4 + 2.8) ％.There was statistically significant difference between anti miR 29 PANC1 cells and other PANC1 cells (P ＜0.05).The survival rate,apoptosis rate,transmembrane cells and migration distance of anti-miR-29 + PUMA-siRNA-PANC1 cells was (84.7 ± 10.9) ％,(1.3 ± 0.8) ％,(49.7 ± 6.4) per 400 high power field and (182 ± 36) μm,indicating that the effects of miR 29 inhibition on PANC1 cells were abolished (all P ＜0.05).The volume of the xenograft of PANC1 and anti-miR-29-PANC1 cells was (3 800 ±270) and (1 890 ± 160)mm3,the cell apoptosis rate was 0.93 ±0.14 and 8.26 ± 1.15,the number of metastatic lung lesions was (26.4 ± 6.5) and (8.6 ± 2.7),the PUMA positivity was (7.2 ±1.6) ％ and (43.8 ± 7.6) ％,E-cadherin positivity was (8.3 ± 3.6) ％ and (47.4 ± 5.7) ％,respectively.The xenograft volume and the number of metastatic lung nodules of anti miR29 PANC1 cells was obviously decreased or decreased,but cell apoptosis rate,PUMA positivity and E cadherin positivity were obviously increased,and the differences were all statistically significant (P ＜ 0.05).Conclusions Inhibiting miR-29 expression can decrease cell proliferation,migration and metastasis of PANC1 cells,and the potential mechanism may be associated with the upregulation of PUMA and E-cadherin.

This paper discussed the current status of home care demand in China from the degree and content of home care demand. It analyzed the influencing factors of residents' home care services acceptance in the aspects of the demographic characteristics, awareness of home care services, health status of residents and lifestyle, so as to provide basic reference for the development of home care service in China.

Objective? To explore the attitude towards patients for patient safety and health literacy among patients with coronary artery bypass graft (CABG) and to analyze the correlation between them. Methods? From September 2017 to May 2018, a total of 210 CABG patients were investigated with the general information questionnaire, Patients for Patient Safety Questionnaire and Health Literacy Management Scale (HeLMS) by convenience sampling to evaluate the attitude towards patient participation as well as patient safety and health literacy. Pearson correlation analysis was used to explore the correlation between them. Results? A total of 209 valid questionaires were collected. Among 209 CABG patients, the average item scores of attitude towards patients for patient safety and health literacy were (3.68±0.20) and (4.14±0.23) respectively. Pearson correlation analysis showed that attitude towards patients for patient safety had a positive correlation with health literacy (r=0.422, P＜0.01). Conclusions? CABG patients are with the low level of attitude towards patients for patient safety and good level of health literacy. Corresponding measures should be taken in clinical work to improve the level of health literacy, attitude towards patients for patient safety safety and the safety of medical care.

Objective:To investigate and analyze status of knowledge, attitude and practice of perioperative prevention of venous thromboembolism (VTE) among Operating Room nurses, so as to provide guidance for VTE prevention.Methods:Using the convenient sampling method, a total of 2 728 Operating Room nurses from 27 provinces, municipalities and autonomous regions in China were investigated with an electronic questionnaire from February to April 2020. The questionnaire included Basic Information Questionnaire, VTE Prevention Status Questionnaire and Perioperative VTE Prevention Knowledge, Attitude and Practice Questionnaire. A total of 2 728 questionnaires were issued, and 2 512 were effectively returned, with an effective recovery rate of 92.08%.Results:79.50% (1 997/2 512) of nurses said that their hospitals had VTE prevention and treatment system and 79.78% (2 004/2 512) of nurses said that their hospitals had relevant training on intraoperative VTE prevention. 67.08% (1 685/2 512) of the nurses' departments had VTE prevention work and 59.95% (1 506/2 512) of the nurses' departments had adequate VTE prevention equipment. The knowledge score, attitude score and behavior score of perioperative VTE prevention questionnaire of Operating Room nurses were (16.18±4.26) , (45.64±4.95) and (78.69±16.36) , respectively. There were statistically significant differences in scores of knowledge dimension of the questionnaire among nurses with different educational background, professional title, position, post and follow-up surgery conditions ( P<0.05) . There were statistically significant differences in scores of attitude dimension of the questionnaire among nurses with different professional titles, positions and follow-up surgery conditions ( P<0.05) . There were statistically significant differences in practice dimension scores of nurses with different ages, working years, educational background, professional title and position ( P<0.05) . Conclusions:Managers should pay attention to the nursing work of preventing VTE in the Operating Room. The Operating Room nurses have insufficient knowledge about perioperative prevention of VTE prevention. Therefore, their prevention attitude needs to be improved and prevention behavior needs to be improved.