As an important mediator of allergic inflammation, mast cell tryptase is involved in the induction of hypersensitivity, infiltration of inflammatory cells and tissue remodeling in respiratory tract. The effects of tryptase inhibitors on the actions of tryptase show further the potential of tryptase in the pathogenesis of asthma and its inhibitors in the treatment of asthma.

A high incidence of esophageal cancer exists in China. Surgical resection remains the dominant therapeutic intervention for patients with operable esophageal carcinoma. However, alternative strategies are actively applied to reduce the frequency of post-op-erative local or distant disease recurrence and to prolong survival. These strategies include neoadjuvant and adjuvant therapies. This review discusses the current knowledge, as well as available data and information, with regard to neoadjuvant and adjuvant therapies for patients with locally advanced esophageal cancer.

Objective To evaluate the health-related quality of life in convalescents of severe acute respiratory syndrome.Methods Health-related quality of life evaluated by SF-36 scale was performed on 68 SARS patients discharged from hospital in the first and sixth month during 2003-05～2003-12 in Department of Respiratory Medicine of Beijing Friendship Hospital.Results In the first month,SARS patients had a significant impairment in both physical and psychological functioning.Physical condition improved significantly six months later,but there was no obvious improvement in mental health.Conclusion Psychological treatment should be included.The SF-36 scale is a valid,sensitive and reliable tool for assessing the quality of life for SARS patients.

To ensure the consistency of genotype results for PowerPlex?21 kit and GoldeneyeTM 20A kit. Methods The STR loci were amplified in DNA samples from 205 unrelated individuals in Beijing Han population. And consistency of 19 overlap STR loci typing were observed. The genetic polymorphism of D1S1656 locus was obtained. Results All 19 overlap loci typing showed consistent. The proportion of peak height of heterozygous loci in two kits showed no statistical difference (P>0.05). The observed heterozygosis of D1S1656 was 0.878. The discrimination power was 0.949. The excluding probability of paternity of triplet was 0.751. The excluding probability of paternity of diploid was 0.506. The polymor-phism information content was 0.810. Conclusion PowerPlex?21 kit and GoldeneyeTM 20A kit present a good consistency. The primer design is reasonable. The polymorphism of D1S1656 is good. The two kits can be used for human genetic analysis, paternity test, and individual identification in forensic practice.

Objective To understand the impact to blood lipid in patients with HIV/AIDS using different HAART regimens . Methods According to HAART regimens ,102 HIV/AIDS patients were divided into two groups :D4T group(D4T+3TC+NVP) and AZT group(AZT+3TC+ NVP) .Blood lipids(TG ,TC ,LDL-C and HDL-C)at baseline ,24 weeks and 48 weeks after HAART were detected .Results Compared with paired t test ,TG ,TC and LDL-C at 24 weeks follow-up of the patients in D4T group were significantly higher than at baseline(P<0 .05) ,and TC at 48 weeks follow-up were still higher than at baseline(P<0 .05) .TG and TC at 24 weeks follow-up of patients in AZT group were significantly higher than at baseline(P<0 .05) .There were no significant difference at blood lipid between the two groups(P>0 .05) .Conclusion There are dyslipidemia of the patients with HIV/AIDS u-sing D4T-based and AZT-based regimens ,and the dyslipidemia are increase of TC and TG ,these increase is border higher .TG and TC at 24 weeks follow-up of the HIV/AIDS patients using D4T-based regimen are increased early ,and TC are still at higher level .

Objective To encode the protein cystatin M with CST6 gene and construct a CST6-overexpression vector,and transfect it into the gastric cancer cells SGC-7901 in order to observe the effect of cystatin M on the proliferation and migration abilities of SGC-7901 cells.Methods There were three groups in the experiment:pcDNA3.1(+)-CST6 group,pcDNA3.1 (+)group and non-transfected group.RT-PCR and Western blot were used to identify the RNA expression of CST6 in SGC-7901/CST6 cells.The proliferation and migration abilities of the transfected cells were detected by MTT and cell wound healing assay,respectively.Results SGC-7901/CST6 cells stably expressed CST6 gene RNA and cystatin M protein.The proliferation and migration of pcDNA3 .1 (+)-CST6 group cells were reduced compared with those of cells in the other two groups (P<0.05).Conclusion Cystatin M can inhibit the proliferation and migration of SGC-7901 cells.

Objective To evaluate the protective effect of Bletilla striata polysaccharide ( BSPS) on immunological and chemical liver injury in mice. Methods Thirty Kunming male mice were randomly divided into five groups, including the normal control group,model control group,and low-,middle-,and high-dose BSPS groups (n=6 each).Tail vein injection of ConA was carried out to establish the ConA-induced liver injury model.After different treatments,all the animals were sacrificed,and the plasma levels of ALT and AST were tested.Additionally,sixty Kunming male mice were randomly divided into six groups,including the normal control group,model control group,silymarin group,and low-,middle-,and high-dose BSPS groups (n=10 each).Tail vein injection of CCl4 was performed to establish the CCl4-induced acute liver injury model.After different treatments,the plasma levels of ALT and GSH were tested.The effects of BSPS on the weights of the liver and spleen were examined. Results The levels of ALT and AST were reduced in BSPS-treated mice when compared with those experiencing only ConA-induced liver injury ( model control group) ,and significant difference was found between the middle-and high-dose BSPS groups and the model control group (P<0.01,P<0.05).The weights of the liver and spleen and the level of ALT were reduced in BSPS-treated mice as compared with those with only CCl4-induced acute liver injury (model control group),while the level of GSH was significantly increased in middle-and high-dose BSPS groups (P<0.05). Conclusion BSPS at low,middle,and high doses can prevent against the ConA-induced immunological liver injury and CCl4-induced acute liver injury in mice.

Objective:To investigate the status of family function of patients after hysterectomy, explore the effect of self-concealment on family function.Methods:From January to November 2021,the 177 patients after hysterectomy in Guangdong Provincial People ′s Hospital were investigated by General Data Questionnaire, Family APGAR Indexand Self-Concealment Scale (SCS), and the influencing factors of family function were analyzed by univariate analysis and binary logistic regression. Results:The total score of APGAR and SCS was 9.00 (6.50, 10.00) and 24.00 (17.00, 33.00) after hysterectomy. Univariate analysis showed that marital status, working conditions and tendency of self-concealment were the influencing factors of family function. In the regression analysis of binary Logistic, tendency of self-concealment( P<0.01) and marital status ( P<0.01) entered the regression equation. Conclusions:The family function of patients after hysterectomy is generally good, and self-concealment is a risk factor. It is suggested that in clinical work, attention should be paid to the tendency of self-concealment in patients after hysterectomy, and targeted intervention measures should be taken to improve their family function.

Objective:To explore the current level of knowledge, attitude and practice of rehabilitation for postpartum diastasis recti abdominis inpuerperae, and analysis the influencing factors of behavior, in order to provide a reference frame for nursing staff to formulate effective health education programs.Methods:From November 2020 to April 2021, a self-designed questionnaire was used to investigate 522 puerperae from 4 tertiary hospitals in Guangdong province, Jiangsu province and Zhejiang province, to explore their level of knowledge, attitude and behavior of rehabilitation for postpartum diastasis recti abdominis. Mann-Whitney Utest or Kruskal-Wallis Htest was used for univariate analysis, Binary Logistic regression analysis was used to analysis the influencing factors of rehabilitative behavior.Results:The median score of knowledgedimension, attitude dimension and behavior dimension of rehabilitation for postpartum diastaisis recti abdominis in puerperae respectively were 47.0 (36.0, 55.0), 26.0 (24.0, 31.0), 15.0 (10.0, 18.0) points. The results show that the main factors influencing of rehabilitation behaviorfor postpartum diastaisis recti abdominis in puerperae were knowledge ( χ2 = 87.78, P<0.05), attitude ( χ2 = 4.77, P<0.05), number of deliveries ( χ2 = 3.94, P<0.05) and family personal monthly income ( χ2 = 4.88, P<0.05). Conclusions:At present, puerperae have a positive attitude towards rehabilitation for postpartum diastasis recti abdominis, but the level of knowledge and practice of rehabilitation for postpartum diastasis recti abdominisneed to be improved.Nursing staff should focus on puerperae with incomplete knowledge, negative attitude, multiple deliveries and low family personal monthly income.

Objective:To investigate the application value of noninvasive prenatal DNA screening combined with nuchal translucency thickness measurement in the diagnosis of fetal chromosome aneuploidy.Methods:A total of 5 730 pregnant women who were screened for fetal chromosomal diseases in the Quzhou Maternal and Child Health Hospital from January 2017 to March 2019 were included in this study. All of them underwent noninvasive prenatal DNA screening and nuchal translucency thickness measurement. The results of amniocentesis were used as the gold standard to evaluate the diagnostic efficacy of noninvasive prenatal DNA screening, nuchal translucency thickness measurement and their combination.Results:Noninvasive prenatal DNA screening revealed that 64 (1.12%) women out of 5 730 pregnant women had high risk of developing chromosomal abnormalities. Ultrasound examination results showed that nuchal translucency was thickened in 140 (2.44%) women. The outcome of adverse pregnancy increased with the increase of nuchal translucency thickness. Among the 68 pregnant women who underwent amniocentesis, 51 women developed chromosomal abnormalities, with trisomy 21 syndrome being the majority (23/51,45.10%). The diagnostic efficacy of noninvasive prenatal DNA screening combined with nuchal translucency thickness measurement in the diagnosis of fetal chromosomal aneuploidy reached the ideal level.Conclusion:Noninvasive prenatal DNA screening combined with nuchal translucency thickness measurement has a high clinical application value. The combined method can be used as the main prenatal DNA screening method for pregnant women and it can effectively avoid the birth of children with chromosomal abnormalities.