Proteomic analysis of core needle biopsy (CNB) sample from patient populations is critical to our understanding of human disease,but has been hindered by its particular small size.Here,we present a method for the proteomic analysis of CNB sample based on the two dimensional electrophoresis.Proteins were extracted directly from 3 rat liver CNB specimens and a human prostate CNB sample.respectively.24 cm Immobiline DryStrip (pH 3-10NL) and 12.5% SDS-PAGE were introduced to separate the proteins.Interesting spots were analyzed by MALDI TOF/TOF mass spectrometry after tryptic digestion.With this method,consistent electrophoretic patterns of more than 2 500 protein spots were reproducibly obtained after silver staining,from rat liver CNB specimens.Qualitatively and quantitatively reproducible results also yield when the method was applied to a human prostate CNB sample.57 stochastically selected protein spots were analyzed by MALDI TOF/TOF moss spectrometry.and were identified with high confidence including faint ones.This simple and reproducible approach raises the opportunity of defining key molecular events of human disease pathologies.

Objective: To explore the treatment effects of edaravone combined with antiviral drugs on 30 adult cases with viral encephalitis. Methods: Sixty adult patients with viral encephalitis in our hospital were selected for the study and were divided into control group and observation group according to the admission number, with 30 cases in each group. Control group was treated with conventional antiviral drugs, and observation group was given antiviral drugs combined with edaravone. Another 30 healthy people admitted to the hospital in the same period for physical examination were included in healthy group. The clinical efficacy and time to normalization of clinical manifestations (fever, headache, vomiting, EEG abnormalities) were recorded in observation group and control group, and the oxidative stress indicators [catalase (CAT), lipids peroxide (LPO)] and nerve injury indexes [neuron-specific enolase (NSE), glial fibrillary acidic protein (GFAP)] were compared between observation group, control group and healthy group before treatment and after 2 weeks of treatment. The neurological deficit [National Institutes of Health Stroke Scale (NIHSS)] was observed in observation group and control group. Results: The clinical efficacy in observation group was significantly better than that in control group (P<0.05), and the normal recovery times of fever, headache, vomiting and abnormal EEG were lower than those in control group (P<0.05). After 2 weeks of treatment, the partial oxidative stress index (serum CAT) in observation group and control group was higher than that before treatment (P<0.05) while partial oxidative stress index (serum LPO), nerve injury indexes (serum NSE, GFAP) and neurological defect (NIHSS score) were lower than those before treatment (P<0.05), and the changes in observation group were greater than those in control group (P<0.05). The serum CAT levels in the two groups before and after treatment were lower than those in healthy group (P<0.05), and the levels of LPO, NSE and GFAP were higher than those in healthy group (P<0.05). Conclusions Edaravone combined with antiviral drugs has significant treatment effects in the treatment of adult viral encephalitis. It can not only reduce the body’ s oxidative stress response, but also reduce nerve injury and improve neurological deficit, and it has a certain application value.

Objective To study the effects of serum C-type natriuretic peptide (CNP) ,insulin-like growth factor II (IGF-Ⅱ ) , endothelin (ET) ,neuron-specific enolase (NSE) and S100B protein(S100B) on the prognosis of the patients with traumatic brain injury .Methods A total of 110 patients with craniocerebral trauma admitted in our hospital from January 2016 to January 2017 se-lected as the craniocerebral trauma group and further divided into the mild ,moderate and severe craniocerebral trauma groups ac-cording to the Glasgow Coma Scale (GCS) .Then the levels of serum CNP ,IGF-Ⅱ ,ET ,NSE and S100B in all cases were analyzed by enzyme-linked immunosorbent assay (ELISA) .Their influence on the prognosis of the patients with craniocerebral trauma and the correlation among various indicators were analyzed .Results The levels of CNP and IGF-Ⅱat admission in the craniocerebral trauma group were significantly decreased ,while the levels of ET ,NSE and S100B were significantly increased ,the difference com-pared with the control group was statistically significant (P<0 .05) .Serum CNP and IGF-Ⅱlevels in the death group ,plant survival group and disabled group were significantly decreased .The difference was statistically significant (P<0 .01) .Serum CNP and IGF-Ⅱlevels in the moderate and severe craniocerebral trauma groups were gradually increased with the disease course progress ,while serum ET ,NSE and S100B levels were gradually decreased with the disease course progress ,the difference was statistically signifi-cant(P<0 .05) .In the patients with craniocerebral trauma ,the positive correlation existed between CNP and IGF-Ⅱ ,between ET and S100B ,between ET and NSE ,and between NSE and S100B(P<0 .01) ,while the negative correlation existed between IGF-Ⅱand ET ,between IGF-Ⅱ and S100B ,between CNP and ET ,and between IGF-Ⅱand NSE (P<0 .01) .Conclusion Serum CNP , IGF-Ⅱ ,ET ,NSE and S100B are correlated to the severity of craniocerebral trauma ,which has a higher clinical application value for judging the disease condition ,evaluating the prognosis in cradiocerebral trauma .