This paper analyzes the factors that affect students' autonomous learning from the aspects of teaching interaction,the binding force of curriculum to students,the application of network and electronic products.On the basis of this,we put forward that taking the molecular biology course of biotechnology major and bio-pharmacy Major as a teaching reform template in our university,and based on the network teaching platform of molecular biology,adopt flipped classroom as the main form to improve students' autonomous learning ability,and promote their ability of lifelong learning,so that they can adapt to the development of pharmaceutical biotechnology industry.

Objective:Molecular biology experimental technology has become an important basic tool for exploring biology and medicine and other related disciplines. We aim to explore an effective molecular biology experimental teaching model which will definitely improve students' molecular biology experimental skills and autonomous learning ability.Methods:Guided by the theory of constructivism, with the molecular biology experimental course as the carrier, and with the basic requirements of constructing the basic molecular biology experimental technology of the system, a teaching platform was established to guide students to preview the experiment independently; the physical experimental projects were integrated and optimized and the virtual simulation experimental projects were increased, with virtuality and reality, to improve students' molecular biology experimental skills and autonomous learning ability.Results:An online teaching platform has been established, which effectively guides and improves the effect of students' preview experiments, and cultivates the ability of autonomous learning. Besides, the experimental teaching mode combining optimization of physical experimental projects and virtual simulation experimental projects significantly improved students' molecular biology experimental operation skills and problem-solving ability.Conclusion:A constructivism-based teaching mode of combining virtual and real molecular biology has been established, which is an effective way to improve students' molecular biology experimental skills and autonomous learning ability.

The molecular biology experiment course plays a bridge role in the biotechnology curriculum system, and has important effect in training students' experimental operation skills, analyzing difficulties and solving problems. This article takes the biotechnology major of local medical colleges as an example to analyze and elaborate the assessment method reform and effect in the teaching process of molecular biology experimental course, aiming at promoting the teaching level of molecular biology experimental courses and improving the training quality of biotechnology professionals.