Objective To evaluate vagina reconstruction with the use of sigmoid colon. Methods 21 cases underwent vagina reconstruction with the use of sigmoid colon were reviewed of which 13 were male pseudohermaphrodism and 8 congenital devoid of vagina. Results The appearence of the reconstructed vagina has been good in all the 21 patients.15 of the 21 have been followed up for 3～24 months with no overproduction of mucus and no need of routine dilatation. Conclusions Vagina reconstruction with the use of an isolated sigmoid loop is an ideal procedure for juveniles and adults.

The metabolic response to stress plays a key role in the adaptive response during critical illness.Multiple mechanisms including the stimulation of the sympathetic nervous system,inflammation,and immune responses were involved.Insulin resistance is one of the main features in metabolic response to stress.Metabolic response to stress was manifested by disorders of energy consumption,such as glucose,lactic acid,lipids,and proteins.The decrease in fat-free body mass and cell mass,relative excess of adipose tissues,and increased extracellular fluid volumes were also involved.Therapeutic interventions,including hormone supplementation,enhanced protein intake,and early mobilization,are considered for prevention and therapy of metabolic response to stress.The review aims to summarize the pathophysiological mechanisms,clinical consequences,and therapeutic implications of metabolic response to stress in critical illness.

Objective To study the CT findings of dermoid cyst of the orbit.Methods Four cases of dermoid cyst of the orbit proved by operation and pathology were presented. There were three cases of male, one case of female, age 6～21 years. Transverse CT scans(3 mm slice) and coronal CT scans(5 mm slice) were obtained, in which enhancement study was done in two cases.Results Among four cases, lesion was located in the right on three cases, in the left on one case. All lesions were located outside the muscle of upper-lateral guarter. One lesion was showed a multi-lobular shape, with widening of the frontozygomatic fissure and protruding to outside of the orbit. Three lesions were found anoval shape, in which one lesion was showed a bony sclerosis and thickening, another two cases were showed no bony change. On all cases, the edge of the lesions were demonstrated clearly, in which three cases were low-density(-140～-80 HU) similar to fat density, and one case with heterogeneous density. The lesion was showed more clearly on enhancement study on two cases.Conclusion CT findings of dermoid cyst of the orbit is characteristic and the diagnosis can be made before operation.

A simple method for culture of human fetal adrenal medullary cells is described. Enzymatically dissociated human fetal adrenal cells were isolaled by centrifugation on Percoll in the buffer. Adrenal medullary cells with 75%-85% purity were obtained by this procedure. The medullary cells were then plated on plastic culture dishes coated with collagen. After 2-7 days medullary Cells began to flatten and extended processes. A further identification of the cells as medullary in origin was confirmed, by specific catecholamine fluorescence technique. Catecholamine content in the medium was also determined by HPLC.

Objective To discuss the treatment of urethral stricture after hypospadias reDair.Methods Thirty-seven cases of urethral stricture after hypospadias repair from 1999 to 2006 were ret-rospectively analyzed. Of 37 patients,the stricture was located at the distal end of urethra in 7 cases.There were 3 1 cases with anastomotic stricture.There was 1 case with distal urethral stricture and anastomotic structure.There were 1 3 patients with the simple urethral stricture and the complicated 24 cases.Of 13 simple urethral stricture patients,4 patients were repaired with urethro-meatomy,6 patients with shelf operation and 3 patients with transurethral holmium laser urethrotomy.Eight Pa-tients with complicated urethral fistula,6 patients with urethral diverticula,4 patients with phallo-campsis and 6 patients with many times of postoperative urethral stricture were repaired with shelf op-eration or penis skin flap or septum of scrotum skin flaps urethroplasty. Results The simple stric-tures of 1 2 patients were cured by stage 1 operation,the other one cured after second surgery.Of 8 patients with fistula,7 were cured at stage 1,the other one was cured at the stage 2.Of 6 patients with diverticulum,all were cured at stage 1.Of 4 patients with phallocampsis,3 cases were cured at stage 1,the other one was cured at the stage 2.Of 6 patients with recurrent strictures,all were cured at stage 1.A1l patients were followed up 1 2 to 24 months.The micturition was without difficuItv at stand position. Conclusions Urethral stricture is one of the most common complications after hypos-padias repair.The simple urethral stricture could be repaired with urethro-meatomy or sheIf operation or transurethral holmium laser urethrotomy.The complicated urethral stricture could be reDaired with shelf operation or penis skin flaps or septum of scrotum skin flaps urethroplasty and overjetted with dortos of scrotum flaps.

Objective:To investigate the effect of ossotide combined with antibiotics on efficacy and serum levels of tumor necrosis factor-alpha (TNF-α), interferon-gamma (IFN-γ) and interleukin-1beta (IL-1β) in patients with brucellosis osteoarthritis.Methods:Using a prospective study, 66 patients with brucellosis osteoarthritis admitted to Jiangshan Beilin Hospital from May 2016 to May 2019 were selected as the study subjects, aged from 28 to 70 years old. The patients were randomly divided into the control group ( n = 33) and the observation group ( n = 33). The control group was treated with antibiotics alone, and the observation group was treated with ossotide injection on the basis of the treatment. Both groups were treated for 2 months. The clinical efficacy was compared between the two groups, the pain visual analogue scale (VAS) scores, the Barthel index of activities of daily living, and the Fugl-Meyer assessment (FMA) scores were compared between the two groups before treatment and 2 months after treatment. The higher the VAS score was, the more severe the pain was. The higher the Barthel index was, the stronger the activity of daily living was. The higher the FMA score was, the better the motor function was. At the same time, the serum levels of TNF-α, IFN-γ and IL-1β were compared between the two groups before treatment and 2 months after treatment. Results:The total effective rate of the observation group (96.97%, 32/33) was significantly higher than that of the control group (78.79%, 26/33), and the difference was statistically significant (χ 2 = 5.12, P < 0.05). After 2 months of treatment, the VAS score [(3.12 ± 0.85) points] of the observation group was significantly lower than that of the control group [(4.08 ± 0.97) points, P < 0.05], Barthel index [(64.72 ± 6.98) points] and FMA score [(58.70 ± 5.58) points] were significantly higher than those of the control group [(56.65 ± 6.72), (51.43 ± 5.33) points, P < 0.05]. After 2 months of treatment, serum levels of TNF-α [(12.30 ± 2.35) ng/L], IFN-γ [(10.87 ± 2.26) ng/L] and IL-1β [(324.28 ± 127.60) ng/L] of the observation group were significantly lower than those of the control group [(14.35 ± 3.13), (15.76 ± 3.21), (390.29 ± 131.37) ng/L, P < 0.05]. Conclusion:Ossotide combined with antibiotics can improve the therapeutic effect of brucellosis osteoarthritis and reduce the serum levels of TNF-α, IFN-γ, and IL-1β.

Objective To explore incidence of diabetes and the metabolic syndrome of rural residents at age of 20 years old or more in Zhongjiang County of Deyang city,and to analyze the re-lated factors.Methods Using multistage random sampling method,a total of 678 people above 20 years old were randomly selected from permanent residents from Zhongjiang County and were con-ducted with questionnaire,anthropometry index measurement and laboratory tests.SPSS statistical software was used to analyze diabetes and the metabolic syndrome.Results The incidence of dia-betes was 7.50% in Zhongjiang County above 20 years or more with 4.42% of men and 9.4% of women.Differences of correlation between gender,age,culture level,obesity,family history of diabetes,lipid metabolism and hypertension and incidence of diabetes were statistically significant (P <0.05).The incidence of the metabolic syndrome was 44.3%.In patients with diabetes, there were 40 people with metabolic syndrome and the incidence was 81.6%.Conclusion Dia-betes and the metabolic syndrome are associated with multiple factors,so prevention and control should be focused so as to reduce the incidence of diabetes and to improve the quality of life.

Objective To explore incidence of diabetes and the metabolic syndrome of rural residents at age of 20 years old or more in Zhongjiang County of Deyang city,and to analyze the re-lated factors.Methods Using multistage random sampling method,a total of 678 people above 20 years old were randomly selected from permanent residents from Zhongjiang County and were con-ducted with questionnaire,anthropometry index measurement and laboratory tests.SPSS statistical software was used to analyze diabetes and the metabolic syndrome.Results The incidence of dia-betes was 7.50% in Zhongjiang County above 20 years or more with 4.42% of men and 9.4% of women.Differences of correlation between gender,age,culture level,obesity,family history of diabetes,lipid metabolism and hypertension and incidence of diabetes were statistically significant (P <0.05).The incidence of the metabolic syndrome was 44.3%.In patients with diabetes, there were 40 people with metabolic syndrome and the incidence was 81.6%.Conclusion Dia-betes and the metabolic syndrome are associated with multiple factors,so prevention and control should be focused so as to reduce the incidence of diabetes and to improve the quality of life.