AIM To observe the relationship between dose effect and time effect on hepatoxicity of Gardenia jasminoides Ellis extract in rats.MOTHODS Wistar rats were divided into four groups:low,middle and high (3,10,and 30 g/kg) dose of G.Jasminoides groups (administrated by gavage),and the normal control group were orally given deionized water.All rats were observed daily during the administration period.On the 7th,14th,28th day after the administration,blood samples were collected;serum alanine transaminase (ALT),aspartate transaminase (AST),alkaline phosphatase (ALP),glutamic dehydrogenase (GLDH) activity and total bile acid (TBA) and total bilirubin (TBIL) were determined.The livers were weighed and the liver index was calculated.HE staining and observation of histopathological changes in the structure of liver tissue under light microscopy were performed.RESULTS After the 7th day of administration,the rats in high dose group showed lower food consumption and slowly increased body weight.Serum ALT,AST,ALP,TBA,TBIL and GLDH in rats from high dose group were significantly higher than those in the normal control group.The liver index of rats in the middle and high dose groups was significantly increased than that in the normal control group.After the 14th day of administration,serum ALT,TBA and TBIL in rats from the high dose group were significantly higher than those in the normal group.The liver index of rats in the middle and high dose groups was significantly increased than that in the normal control group.After 28th day of administration,serum ALT and TBA in the rats from the high dose group,TBIL and GLDH in rats from the middle dose group,and GLDH in rats from the low dose group were significantly higher than those in the normal control group.The liver indexes of rats in all dose groups were significantly increased than those in the normal control group.After the 7th,14th or 28th day of the treatment,histopathological changes such as the liver cell hypertrophy,interlobular bile duct hyperplasia,and inflammatory cell infiltration appeared in the middle and high dose groups.CONCLUSION The high dose of G.jasminoides can induce and increase liver toxicity with the increase in dose,but at high dose level,liver toxicity does not increase with time.

Objective To study effect of time choosing of urine tube extubation after general anesthesia intracranial stents implantation.Methods A total of 82 patients with intracranial artery stenosis were randomly divided into control group and observation group,with 41 cases in each group.The observation group pulled out urinary tube when the anesthesia Steward to score was 4 points,the control removed extubation after 24 h when lifting side limbs got out of braking.Headaches,high blood pressure,disturbance of consciousness,seizures,bleeding in the brain,such as clinical symptoms were compared,TCD monitoring results and urine output and urination method for the first time were recorded.Results Observation group had lower incidence of headache,disturbance of consciousness,seizures and other clinical symptoms than the control group,the proportion of patients with the blood pressure ＞ 150 MMHG and TCD monitoring with ipsilateral middle cerebral artery blood flow velocity of 1.5 ～ 3 times increase was significantly higher than control group (P ＜ 0.05);There was no significant difference in the first time urination volume,the success rate of urination and induced urination success rate,and urination success rate was higher,there was no patients with urethral catheterization.Conclusion For patients with line intracranial stent angioplasty general anesthesia,the preoperative nursing can guide bed urination,placing a urinary catheter can increase susceptibility of the adverse reactions caused by urinary catheter.And it can effectively avoid urinary retention and urinary irritation caused by high cerebral perfusion syndrome and other complications,greatly improve the patient's comfort,and no adverse effects of patients with urination is observed.

Objective To study effect of time choosing of urine tube extubation after general anesthesia intracranial stents implantation.Methods A total of 82 patients with intracranial artery stenosis were randomly divided into control group and observation group,with 41 cases in each group.The observation group pulled out urinary tube when the anesthesia Steward to score was 4 points,the control removed extubation after 24 h when lifting side limbs got out of braking.Headaches,high blood pressure,disturbance of consciousness,seizures,bleeding in the brain,such as clinical symptoms were compared,TCD monitoring results and urine output and urination method for the first time were recorded.Results Observation group had lower incidence of headache,disturbance of consciousness,seizures and other clinical symptoms than the control group,the proportion of patients with the blood pressure ＞ 150 MMHG and TCD monitoring with ipsilateral middle cerebral artery blood flow velocity of 1.5 ～ 3 times increase was significantly higher than control group (P ＜ 0.05);There was no significant difference in the first time urination volume,the success rate of urination and induced urination success rate,and urination success rate was higher,there was no patients with urethral catheterization.Conclusion For patients with line intracranial stent angioplasty general anesthesia,the preoperative nursing can guide bed urination,placing a urinary catheter can increase susceptibility of the adverse reactions caused by urinary catheter.And it can effectively avoid urinary retention and urinary irritation caused by high cerebral perfusion syndrome and other complications,greatly improve the patient's comfort,and no adverse effects of patients with urination is observed.

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