OBJECTIVETo study the preparation ofnanostructured lipid carriers loaded with supercritical carbon dioxide fluid extraction of Xionggui powder.

METHODPrepared nanostructured lipid carriers (NLC) loaded with supercritical carbon dioxide fluid extraction of Xionggui powder (XG-CO2-SFE) with a microemulsion ultrasonication method , established the best prescription of XG-CO2-SFE-NLC by orthogonal design methods with entrapment efficiency of nanoparticles as index, and investigated their physicochemical characterizations.

RESULTThe best prescription was m(phospholipid) : m(S-40) : m(lipid carriers) = 5 : 2 : 1, the entrapment efficiency of nanopartices was 86.8%, results revealed that nanoparticles were sphere like with the mean size of 92.6 nm and the mean zeta potential was -20.68 mV.

CONCLUSIONThe prep aration method of the XG-CO2-SFE-NLC is appropriate.

Carbon Dioxide ; chemistry ; Chromatography, Supercritical Fluid ; Drug Carriers ; chemistry ; Drugs, Chinese Herbal ; chemistry ; isolation & purification ; Emulsions ; Linear Models ; Lipids ; chemistry ; Nanostructures ; chemistry ; Powders

3' Untranslated Regions ; Animals ; Biomarkers ; metabolism ; Cell Proliferation ; Cell Survival ; Embryonic Stem Cells ; cytology ; metabolism ; Induced Pluripotent Stem Cells ; cytology ; metabolism ; Mice ; Swine ; Transcription Factors ; genetics ; metabolism