Objective]To observe the effect of abdominal acupuncture combined with body acupuncture on chloasma of stagnation of the liver-type. [Method] 30 patients were randomized into treatment group and control group, 15 in each. Treatment group were given abdominal needle combining acupuncture treatment, control group were only given acupuncture treatment. Each group were treated once a day, 5 times made a course, after each course had a rest for 2 days. After 4 courses, to observe the effect and the skin color score, and area score and declined index. [Results]After treatment, the two groups were effective, but the treatment group was more obvious, and scores difference compared with the control group was statistically significant(P<0.01). The total effective rate was 93.3% in the treatment group 86.67% in the control group, there was a significant difference between the two groups(P<0.05). [Conclusion]Abdominal hepatic stagnation type treated by acupuncture combined with body acupuncture on chloasma treatment, no pain or abdominal pain to a lesser degree, even more easily accepted by patients, is better than body acupuncture.

OBJECTIVE To improve the competition ability of the hospital,emphasis of the importance of hospital infection management is necessary and urgent.METHODS Our experience on improving culture level in hospital infection management was introduced.RESULTS We improved management efficiency by fluent information,altered the concept of value by bettered the management means,and brought the renovation idea by lifting the qualification of management staff.CONCLUSIONS Correctly making the most use of the unique principles of culture in hospital infection management could be benefit for creating a good atmosphere,establishing nice profession image,and setting up norm criterion,which could facilitate the controlling hospital infection.

Objective To observe the sleeping time and analyze its impact on the psychological state and quality of life of patients with type 2 diabetes .Methods Convenience sampling was used to recruit 365 patients with type 2 diabetes in Jiangsu Province Hospital on Integration of Chinese and Western Medicine Affiliated to Nanjing University of Chinese Medicine between April 2014 and April 2015 .All the patients were divided into two groups according to the six-hour cut-off point of sleeping time .They were investigated by means of World Health Organization Quality of Life-BREF ( WHOQOL-BREF), Diabetes Distress Scale ( DDS) and General Self-Efficacy Scale ( GSES).Data were analyzed by t-test, chi-square test, Mann-Whitney U test and multivariate regression analysis .Results The average sleeping time was 7.03 h, and the sleeping time of 109 patients was less than 6 h.Patients with less sleeping time had lower quality of life and self-efficacy scores as well as higher diabetes distress scores than those with sleeping time more than 6 h.The differences were statistically significant (P<0.05).Regression analysis showed that sleeping time was the factor of quality of life (β=0.117, P=0.047), self-efficacy (β=0.136, P=0.024) and diabetes distress(β=-0.118, P=0.046).Conclusion Sleeping time affects the psychological state and quality of life of patients with type 2 diabetes.The medical should pay more attention to the quality of sleep of such patients .

Objective To explore the relationship of perceived professional benefit, job satisfaction, and retention willingness of nurses. Methods Convenience sampling method was adopted to investigate 493 nurses from 3 tertiary grade A hospitals in Taian. Results The mean scores of nurses′perceived professional benefit, job satisfaction, and retention willingness were 134.19 ± 19.79,121.52 ± 18.41, and 21.18 ± 4.23, respectively. Perceived professional benefit and job satisfaction were positively correlated with retention willingness (P<0.01). Job satisfaction played a partial mediation role in the relationship between perceived professional benefit and retention willingness. Conclusion Job satisfaction and perceived professional benefit have a positive influence on nurses′retention willingness .

Objective To investigate the feasibility of internal mammary sentinel node(SN) biopsy and internal mammary lymph chain excision under thoracoscope. Methods Six small pigs were used for the experiment.The small pigs were intubated using a double lumen endotracheal tube and kept anesthetized with ketamine.Methylene blue(4 ml) was injected subdermally into both of the first pair of breast.The sentinel node biopsy and internal mammary lymph chain dissection was performed thoracoscopically using a harmonic scalpel.Time of the appearance of the first blue-stained node and the dissection procedure were recorded respectively.Results Thoracoscopic internal mammary sentinel node dissection and internal mammary lymph chain excision was performed successfully in all the 6 pigs(12 sides).The time of the appearance of the first blue-stained sentinel node was 5~15 min(mean,8.9 min),the time of thoracoscopic biopsy was 15~50 min(mean,30.4 min),and the time of internal mammary lymph chain excision was 30~56 min on the left side and 22~48 min on the right side(mean,38.2 min),respectively.No bleeding,lung injuries,and other surgical complications occurred. Conclusions Thoracoscopic internal mammary sentinel node dissection and internal mammary lymph chain excision is feasible,easy to perform,and minimally invasive.

Objective To investigate the antimicrobial resistance of pathogens causing complicated urinary tract infection. Methods Distribution and resistance of pathogens in 260 culture-positive hospitalized complicated urinary tract patients from 1995 to 2001 were analyzed. Results Gram-negative bacilli accounted for 77. 3% , with 44. 2% of E. Coli. Gram-positive cocci accounted for 16. 9% , with 11. 6% of Enterococci; and fungi accounted for 5. 8%. E. Coli had the highest resistance rate of 84. 1% for ampicillin. Resistance rate of E. Coli to amoxicillin/clavulate(21. 3% ) was significantly lower than that to ampicillin alone ( P

Objective To study the pathological characteristics and outcomes of IgA nephropathy(IgAN)presented with nephrotic syndrome (NS) and its relationship with prognosis. Methods From 1987 to 2002,clinical and pathological characteristics of IgAN presented with NS and its response to glucocorticoids therapy were reviewed and compared to non-nephrotic group. Results 7 ^1%(51/723) of IgAN presented as NS.The prevalence of hypertension and renal insufficiency was significantly higher in nephrotic group than that of non-nephrotic group(35 ^3% vs 13 ^8% and 47 ^1% vs 19 ^2%, P

Objective The study aimed to improve the medication safety of aged patients through the reengineering of the dispense process of discharge medications for elderly cardiac patients.Methods The original dispense process was analyzed to find the existing problems,changing the order of process,increasing or decomposing steps of process in order to redesign,perfect and implement the distribution process of medications.Time and correction rate of drug dispensing for three times a day,mastering degree of related knowledge of oral medication and satisfaction degree with medication instruction were compared before and after reengineering of the process.Results Compared with the original process,patients' ability to understand the drug knowledge,the timing when the patients take three medications per day and the satisfaction degree with the instructions of taking medications all had statistical significance.Conclusions Reengineering of the dispense process of discharge medications for elderly cardiac patients can help to improve patients' understanding of drug knowledge,improve the security of taking medications outside the hospital as well as their satisfaction degree with nursing.

Objective To investigate the expression changes of SSR in the process of cardiac remodeling.Methods Myocardial infarction (MI) was induced by left anterior descending coronary artery ligation in mice to establish cardiac remodeling model.Mice subjected to isoproterenol (ISO) subcutaneous injection for 2 weeks to establish acute cardiac injury model.Mice subjected to aortic banding (AB) to establish a mouse model of cardiac hypertrophy.RT-PCR was used to detect the expression change of SSR in various cardiac remodeling models.Results The expression levels of SSR subunit 1 (SSR1) and 3 (SSR3) were significantly decreased in mice after 2 weeks of MI (P ＜ 0.05),and were also decreased in acute cardiac injury induced by 2 weeks of ISO injection (P ＜ 0.05),and reduced afterl week of AB operation (P ＜ 0.05).However,the expression of SSR1 and SSR3 increased at 2 weeks after AB (P ＜ 0.05),and sustained to 8 wccks after AB (P ＜ 0.05).Conclusion The expression of SSR3 and SSR1 in different models of cardiac remodeling were significantly changed,and showed dynamic changes,suggesting that it may participate in the occurrence and development of cardiac remodeling.

The 13 nm gold nanoparticles ( AuNPs ) were synthesized through the reduction of HAuCl4 by sodium citrate and the glutathione was assembled on the AuNPs. Under the experiment conditions, glutathione-modified AuNPs ( GSH-AuNPs ) with negative charge presented a wine red color owing to the electrostatic repulsion between nanoparticals. Upon the addition of neurogenin 3 ( ngn3 ) peptide, the aggregation of GSH-AuNPs was induced by ngn3 peptide under a certain concentration of salt. The color of AuNPs solution changed from red to blue as a function of the ngn3 peptide concentration. Thus, a rapid detection method for ngn3 peptide using GSH-AuNPs as colorimetric probe was established. The optimal experiment conditions were equilibrium time=10 min, pH=6. 0, CNaCl=100 mmol/L. Under the optimum conditions, the assay showed a linear response range of 20-300 μg/L for the peptide with a detection limit being 8 μg/L and exhibited excellent selectivity for ngn3 peptide.