1.Differential expression of lncRNA SH3BP5-AS1 in hepatocellular carcinoma and its mechanisms of promoting hepatocarcinogenesis
Dong Xu ; Huanhua Lu ; Xiaoliang Wang ; Weixin Wu
Acta Universitatis Medicinalis Anhui 2022;57(6):872-877
Abstract:
To investigate the differential expression of lncRNA SH3BP5-AS1 in hepatocellular carcinoma and the possible mechanism of promoting hepatocarcinogenesis.
Methods:
The expression of SH3BP5-AS1 in hepatocellular carcinoma tissue/paraneoplastic tissue(n=91) was detected by qRT-PCR. TCGA database visualization software UALCAN analyzed SH3BP5-AS1 expression in a large sample of hepatocellular carcinoma tissues(n= 371). The expression of SH3BP5-AS1 was detected by qRT-PCR in normal hepatocytes THLE-2 and hepatoma cell lines Huh 7, BEL-7405, SNU-387, Hep 3 B. After silencing SH3BP5-AS1 expression by siRNA technology, the effects of silencing SH3BP5-AS1 on the proliferation of Hep 3 B cells were detected by CCK-8 assay, cell clone formation assay and Edu assay, and the effects of silencing SH3BP5-AS1 on apoptosis and cycle of Hep 3 B cells were detected by flow-through assay. Based on the expression of SH3BP5-AS1 and TM6 SF2 in hepatocellular carcinoma tissues, the expression correlation was counted and analyzed.
Results:
SH3BP5-AS1 was highly expressed in hepatocellular carcinoma tissues. SH3BP5-AS1 was highly expressed in a large sample of hepatocellular carcinoma tissues from the TCGA database SH3BP5-AS1 was highly expressed in different hepatocellular carcinoma cell lines Huh 7, BEL-7405, SNU-387 and Hep 3 B to a different extent. Silencing of SH3BP5-AS1 by siRNA inhibited the proliferation ability of Hep 3 B cells, promoted apoptosis ability, affected the cell cycle and increased the number of G1 phase cells. SH3BP5-AS1 and TM6 SF2 expression were negatively correlated in hepatocellular carcinoma tissues.
Conclusion
SH3BP5-AS1 is an important oncogenic non-coding RNA that can provide a theoretical basis for tumour-targeted therapy in patients with hepatocellular carcinoma.
2.Influencing factors of chronic dyslipidemia in T2DM patients with diabetes point system management in Qingpu District，Shanghai
Zhouli WU ; Sen WANG ; Jie CHEN ; Zhihua REN ; Kaiyou YE ; Yueqin JIANG ; Ya WU
Journal of Public Health and Preventive Medicine 2025;36(2):56-60
Objective To understand the influencing factors of chronic dyslipidemia in T2DM patients who signed a contract for diabetes point system management in Qingpu District, and to provide a basis for comprehensive intervention and prevention and control of dyslipidemia in T2DM patients and to optimize the management strategy of Qingpu District diabetes point system. Methods Among the T2DM patients who signed the diabetes point system from 2017 to 2023, patients with chronic dyslipidemia and normal blood lipids were selected and included in the case group and the control group, respectively. A case-control study was conducted with 1:1 matching by age and gender to analyze the factors influencing dyslipidemia. Results Multifactorial paired logistic regression analysis showed that overweight/obesity and central obesity and smoking in T2DM patients increased the risk of dyslipidemia by 1.93, 2.27, and 2.16 times, respectively. Long-term use of lipid-lowering drugs, duration of diabetes for 5 years or more, regular physical exercise, knowledge of blood lipid status, and married status could reduce the risk of dyslipidemia in T2DM patients (OR values were 0.547, 0.452, 0.685, 0.386 and 0.354, respectively). Current complications (history of stroke, coronary heart disease, and renal insufficiency) were also associated with dyslipidemia (OR=1.802, 95% CI:1.125-2.888). Conclusion The management of diabetes point system in Qingpu District should strengthen the feedback and interpretation of blood lipid monitoring results, improve patients’ health awareness of blood lipid management, and actively take comprehensive management of lifestyle intervention and drug treatment to effectively control blood lipid and reduce the occurrence of related complications.
3.Influencing factors of chronic dyslipidemia in T2DM patients with diabetes point system management in Qingpu District，Shanghai
Zhouli WU ; Sen WANG ; Jie CHEN ; Zhihua REN ; Kaiyou YE ; Yueqin JIANG ; Ya WU
Journal of Public Health and Preventive Medicine 2025;36(2):56-60
Objective To understand the influencing factors of chronic dyslipidemia in T2DM patients who signed a contract for diabetes point system management in Qingpu District, and to provide a basis for comprehensive intervention and prevention and control of dyslipidemia in T2DM patients and to optimize the management strategy of Qingpu District diabetes point system. Methods Among the T2DM patients who signed the diabetes point system from 2017 to 2023, patients with chronic dyslipidemia and normal blood lipids were selected and included in the case group and the control group, respectively. A case-control study was conducted with 1:1 matching by age and gender to analyze the factors influencing dyslipidemia. Results Multifactorial paired logistic regression analysis showed that overweight/obesity and central obesity and smoking in T2DM patients increased the risk of dyslipidemia by 1.93, 2.27, and 2.16 times, respectively. Long-term use of lipid-lowering drugs, duration of diabetes for 5 years or more, regular physical exercise, knowledge of blood lipid status, and married status could reduce the risk of dyslipidemia in T2DM patients (OR values were 0.547, 0.452, 0.685, 0.386 and 0.354, respectively). Current complications (history of stroke, coronary heart disease, and renal insufficiency) were also associated with dyslipidemia (OR=1.802, 95% CI:1.125-2.888). Conclusion The management of diabetes point system in Qingpu District should strengthen the feedback and interpretation of blood lipid monitoring results, improve patients’ health awareness of blood lipid management, and actively take comprehensive management of lifestyle intervention and drug treatment to effectively control blood lipid and reduce the occurrence of related complications.
4.Analysis of the incidence trend of thyroid cancer in Qingpu District of Shanghai from 2007 to 2016
Yueqin WANG ; Zhouli WU ; Sen WANG ; Ya WU ; Ye LU
Shanghai Journal of Preventive Medicine 2022;34(4):378-381
ObjectiveTo understand the incidence and change trend of thyroid cancer in Qingpu District， Shanghai from 2007 to 2016， and to provide a reference for formulating relevant prevention and treatment strategies. MethodsThe incidence data of thyroid cancer among residents in Qingpu District from 2007 to 2016 were collected using the Shanghai Malignant Tumor Registration System， and the crude incidence rate was calculated for different gender and age groups. The age-standardized incidence rate was calculated using the world standard population， and the annual percentage change （APC） was calculated using Joinpoint software to analyze the time trend of the incidence of thyroid cancer. ResultsThe incidence of thyroid cancer changed from 8.97/105 in 2007 to 52.02/105 in 2016， with an increase of 479.93% （APC=23.60， P<0.05）. The standardized incidence rate of thyroid cancer in men and women was 11.21/105 and 32.11/105， respectively， Male to female incidence ratio was 1∶3. Thyroid cancer in Qingpu District was mainly concentrated in the 25‒64 age group， accounting for 88.03% of the total incidence. ConclusionFrom 2007 to 2016， the incidence of thyroid cancer in Qingpu District， Shanghai has a significant upward trend. The age of high incidence tends to be younger and the incidence of women is significantly higher than that of men. Corresponding prevention and treatment measures should be formulated in consideration of the discovered incidence characteristics.
5.Analysis of monitoring results of disinfection quality in child care institutions in Qingpu District of Shanghai， 2016-2018
Yu-feng WANG ; Tian LIU ; Yin-jun PAN ; Zhi-hua REN ; Jia-xin CHEN ; Pei-feng ZHU
Shanghai Journal of Preventive Medicine 2021;33(10):974-977
Objective:To determine the disinfection effect in child care institutions in Qingpu District of Shanghai， and provide evidence for improving the disinfection quality and preventing and controlling infectious diseases. Methods:According to the monitoring program of disinfection quality in child care institutions in Shanghai， disinfection quality was monitored for 3 years. Results:A total of 979 samples were monitored in the consecutive three years， in which 895 samples were qualified with a total qualification rate of 91.42%. The qualification rate of air samples was 100.00%， while the rate of staff hand samples was the lowest， with an average of 79.60%. During 2016-2018， the difference among the annual qualification rates was statistically significant（
6.Analysis of life expectancy and cause-eliminated life expectancy of the registered residents in Qingpu District， Shanghai， 2002‒2021
Rongrong HAN ; Shan JIN ; Liping FANG ; Wu WANG ; Jieyu ZHANG ; Bo CAO ; Xingxing XU
Shanghai Journal of Preventive Medicine 2024;36(9):878-882
ObjectiveTo analyze the trends in life expectancy and cause-eliminated life expectancy of the registered residents in Qingpu District, Shanghai from 2002 to 2021, and to identify the major diseases causing life expectancy loss in the area, so as to provide a theoretical basis for strengthening disease prevention and interventions and optimizing health resources allocation. MethodsUsing the International Classification of Diseases (ICD)-10 classification of death causes, average life expectancy and cause-eliminated life expectancy were calculated with the abbreviated life table and the cause-eliminated life expectancy table. A trend forecast of life expectancy from 2022 to 2029 was made. ResultsFrom 2002 to 2021, the life expectancy of Qingpu District residents showed an increasing trend, with an average annual growth of 0.32 years (AAPC=0.39%, P<0.001). Among the seven major causes of death, the cause-eliminated expected life expectancy for circulatory system diseases showed the most significant upward trend (AAPC=0.53%, P<0.001), rising from the third leading cause of death in 2002 to the first in 2021. The life expectancy loss rate due to circulatory system diseases increased from 3.79% to 7.97%. Respiratory system diseases showed the largest decline, decreasing from the first leading cause of death in 2002 to the fourth in 2021, with the life expectancy loss rate decreasing from 6.83% to 0.99%. ConclusionLife expectancy of the registered residents in Qingpu District has reached a leading level in China. Future efforts should focus on effectively increasing the life expectancy of male residents, strengthening the comprehensive prevention and treatment of chronic diseases such as cerebrovascular diseases, diabetes, and malignant tumors, and improving health promotion policies for residents to achieve a steady long-term increase in regional life expectancy.
7.Analysis of the epidemiological characteristics and survival rate of cancer in Qingpu District， Shanghai from 2012 to 2016
Chun-fang XU ; Ya WU ; Sen WANG ; Yue-qin WANG ; Zhou-li WU
Shanghai Journal of Preventive Medicine 2021;33(7):583-587
Objective:To analyze the incidence and death characteristics of cancer and survival rate of residents in Qingpu District， Shanghai from 2012 to 2016， and to provide a scientific basis for subsequent cancer prevention and treatment. Methods:The malignant tumor incidence and death data were collected by the Shanghai Malignant Tumor Case Registration System. Based on these data， the crude incidence and mortality rate of cancer， the standardized rate， the order of incidence and death of the top 10 cancers， and the overall survival rate were calculated using the life table method. The relative survival rate for 1 to 5 years was calculated using the Ederer II method according to survival probability in the abbreviated life table for the same period. The annual percentage change （APC） was calculated by Joinpoint software， and the trend of morbidity and mortality was analyzed. Results:A total of 10 893 new malignant tumor cases were diagnosed in the region from 2012 to 2016. The crude incidence rate of cancer in the whole region was 464.68/100 000 （502.06/100 000 for men and 428.57/100 000 for women）， and the standardized incidence rate was 224.61/100 000 （235.52/100 000 for men， and 217.98/100 000 for women）. A total of 5 820 people died of cancer， the crude mortality rate of cancer in the whole region was 248.28/100 000 （321.92/100 000 for men and 177.13/100 000 for women）， and the standardized mortality rate was 97.08/100 000 （133.14/100 000 for men and 66.38/100 000 for women）. Patients with malignant tumors diagnosed in Qingpu District in 2012 showed higher 5-year relative survival for breast cancer （87.03%）， malignant tumors of brain and central nervous system （73.62%）， and colorectal cancer （58.22%）. A relative low 5-year survival rate was observed in patients with pancreatic cancer （3.76%）， esophageal cancer （10.55%）， and liver cancer （15.79）. Conclusion:Lung cancer and malignant tumors of the digestive system （including gastric cancer and colorectal cancer） are the main types of cancer threatening the health of residents in Qingpu District. The survival rates of breast cancer， malignant tumors of brain and central nervous system， and colorectal cancer have reached or approached the level of developed countries. However， the overall survival rate of malignant tumors in Qingpu District is lower than that in the city， and needs to be further improved.
8.In vitro release study on self-microemulsion of curcumin and piperine
Jinmei REN ; Man LI ; Jing WANG ; Shenyong GU
Journal of Pharmaceutical Practice 2022;40(1):66-69
Objective To study the release profile of curcumin and piperine from the compound self-microemulsion. Methods The release of curcumin and piperine in vitro was investigated by dynamic dialysis under the condition of phosphate buffer of pH 4.8 and 7.5 with 0.75% Tween-80. Results The cumulative release rates of curcumin in pH 4.8 and pH 7.5 were 94.85% and 84.38% in 108 h, respectively. The cumulative release rates of piperine were 92.85% and 90.05% in 36 h, separately. Conclusion Curcumin and piperine in self-microemulsion have sustained release properties and released more in the acidic environment similar to the environment in tumors.
9.Characteristics and trend analysis of injury causes of death in Qingpu District of Shanghai，2002‒2020
Wu WANG ; Liping FANG ; Shan JIN ; Rongrong HAN ; Jieyu ZHANG ; Xingxing XU
Shanghai Journal of Preventive Medicine 2024;36(6):606-610
ObjectiveTo analyze the characteristics and changing trend of injury cause of mortality of residents in Qingpu District from 2002 to 2020， and to provide scientific reference for formulating regional prevention and control measures. MethodsThe injury mortality data of the registered residents in Qingpu District from 2002 to 2020 were collected. The indicators such as crude mortality rate， standardized mortality rate， and the ranking of causes of death were calculated. ResultsFrom 2002 to 2020， the average annual crude mortality rate was 50.27/100 000， the age-standardized mortality rate based on the world standard population（ASRW） was 30.08/100 000， and the age-standardized mortality rate based on the 2010 Chinese census（ASMRC） was 35.58/100 000. The average annual crude mortality rate of males was higher than that of females ［Z=54.402， Mantel-Hanszel χ2=1 742.509， P<0.01）. The overall injury mortality rate showed a downward trend with an average annual percent change（AAPC）of -4.07% （95%CI： -5.23%‒-2.90%）， P<0.001］. The top four causes of injury death were transportation accident， indeliberate fall， drowning， and suicide. The leading causes of death in 0‒ years old， 15‒ years old and ≥65 years old were drowning， transportation accident and indeliberate fall， respectively. The ASRW of transportation accident， drowning and suicide all showed a decreasing trend， and the AAPC were -8.22% （95%CI： -10.16%‒-6.24%）， -6.99% （95%CI： -9.68%‒-4.22%） and -6.21% （95%CI： -9.38%‒-2.94%）， respectively. ConclusionThe injury death rate of residents in Qingpu District shows a decreasing trend， and the distribution characteristics of injury death are different among different genders and age groups. Corresponding prevention and control strategies should be adopted for different populations.
10.Current situation and trend of medical laboratory results homogeneity management.
Jin Jin WANG ; Li Ming XU ; Wan Jun YU ; Qing KE ; Qian GONG
Chinese Journal of Preventive Medicine 2023;57(9):1504-1509
Medical test results are indispensable and important tools in diagnosis and treatment services. It is necessary to promote the homogenization of test results first, because homogenization is the basis for mutual recognition of test results. Mutual recognition of medical test results can help share resources among medical institutions, provide more reliable test results for early prevention, screening and treatment of diseases, and reduce repeated tests, thus improving people's medical experience. In recent years, with the deepening of medical system reform and the promotion of graded diagnosis and treatment, governments have continuously introduced policies of mutual recognition of test results around country. However, homogenization is a prerequisite for mutual recognition of test results, with the emergence of intelligent medicine in the era of internet big data, opportunities and challenges coexist in the development of homogeneity management. In the future, the homogeneity of medical test results will present a trend of digitalization, automation, informatization and intelligence.
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