Objective:To study CT and MRI radiological expression of benign and malignant meningiomas to provide a reliable evidence for clinical surgery scheme determination. Methods: The radiological and pathological data of 40 cases with meningiomas undergoing surgery in hospital were analyzed, and the benign and malignant lesions of meningiomas were judged according to imaging features such as meningioma shape, edge, signal or density, peritumorous edema, intratumorous calcification, multiple tumors, tumor brain interface status etc.Results: (1)Tumor signal or density: T1 signal inhomogeneous in 6 cases(15.0%), T2 signal inhomogeneous in 8 cases(20.0%), the signal homogeneous in 26 cases (65.0%), CT density is inhomogeneous in 15 cases(37.5%), CT density is homogeneous in 25 cases(62.5%); (2)Tumor edge: regular in 27 cases(67.5%), coarse, irregular, lobulated, with mural nodules in 13 cases(32.5%); (3)Dural tail sign in 36 cases (90.0%); (4)Skull invasion in 6 cases(15.0%); (5)Calcification within the tumor in 7 cases(17.5%); (6)Peritumoral edema in 14 cases(35.0%); (7)Multiple tumors in 6 cases(15.0%); (8)Enhance performance: Obvious homogeneous enhancement in 27 cases(67.5%), Obvious inhomogeneous enhancement in 13 cases(32.5%).Conclusion: Benign and malignant meningiomas with a certain radiological feature, the curative effect and prognosis may be properly evaluated by the radiological expression of meningiomas.

Objective:To compare the optimal conditions, virus yield, viral titer and cell metabolism between culturing influenza virus H1N1 vaccine strain in MDCK and MDCK-G1 cells.Methods:The optimal culture conditions were investigated using chessboard method. The hemagglutination titer, half of the tissue infection dose (TCID 50) and the metabolism of glucose and lactic acid were monitored and compared between the two cell lines. Results:After MDCK-G1 cells were inoculated with H1N1 at the multiplicity of infection (MOI) of 0.001 with the presence of 1 μg/ml of trypsin, the hemagglutination titer reached the peak of 1∶512 at 72 h and the viral titer was 10 7.4TCID 50/ml. In the MDCK cell line group, the hemagglutination titer reached the peak of 1∶256 at 72 h and the viral titer was 10 6.6TCID 50/ml when using H1N1 at MOI=0.0001 and 1 μg/ml of trypsin. Conclusions:MDCK-G1 cells were more suitable than MDCK cells for the proliferation of influenza virus. This study provided reference data for further research on cell-derived influenza vaccine.