ObjectiveTo investigate the intervention of level of atorvastatin calcium tablets (lipitor) on soluble CD40 (CD40L) and high sensitivity C-reactive protein (hs-CRP) in patients with angina.Methods 65 patients with coronary heart disease were divided into stable angina (SA group,n= 30) and unstable angina (UA group,n= 35).The vein blood was collected in the hospitalized morning,2,4,6 weeks after treatment and serum levels of hs-CRP and CD40L were measured and compared between the two groups.ResultsThe levels of soluable CD40L [(20.52± 2.91)μg/L] and CRP [(7.96±1.69) mg/L] in UA group were higher than SA group [(7.96±-1.35) tg/L and ( 1.58 ± 0.91 ) mg/L] (t = 21.705、18.493,both P＜ 0.05) before treatment,and after treatment,their levels in the two groups were significantly lower than pre-treatment (P＜0.01).ConclusionsCD40L and hs-CRP may involved in the pathophysiology of unstable angina process and can be used as an indicator reflecting vulnerable plaque.Lipitor might enhance stability of atherosclerotic plaque and prevent acute coronary events by reducing levels of CD40L and hs-CRP.

Objective To observe the dynamic expression and location of TGF-β1 and TGF-β receptor Ⅰ ,Ⅱ at different time in an experimental model of rat molars reversible pulpitis, to evaluate the role of TGF-β1 and TGFβ receptor Ⅰ , Ⅱ on pulp injury and repair. Methods The reversible pulpitis animal model was established by drilling the enamel without water cooling and then acid etching dentine. The maxillary and teeth of each animal were made 5μm thick serial slides. The slides were stained with hematoxylin-eosin and SABC immunehistochemical technique and then studied under the light microscope and under image analysis, all the data were statistically analyzed by analysis of variance (ANOVA) and t-test. Results Positive staining of TGF-β1 and TβR Ⅰ ,TβR Ⅱ was found in odontoblasts, pulp fibroblasts and vascular endothelial cells. In pulpitis stage,the expressions of TGF-β1 and TGF-β receptor Ⅰ , Ⅱ were increased markedly, and showed dynamic changes at different time;TGF-β1 show a peak at 5d and followed by decreased significantly. TGF-β receptor Ⅰ ,Ⅱshowed the highest at 3d ,and then decreased slightly. From 0d to 3 d,TGF-β1 and TGF-β receptorⅠ,Ⅱ showed uptrends, but their uptrends had no direct proportion relationship. Conclusion The results presented here suggested that TGF-β1 and TGF-β receptorⅠ,Ⅱ might play an important role in pulp wound and repair. The inflammatory reaction in response to TGF-β1 was mediated by TGF-β receptorⅠ, Ⅱ; Although TGF-β1 showed a possible function for the TGF-β receptor,but a direct proportion relationship couldn't be concluded.

AIM: To investigate the produce of intracellular cytokine following short-term in vitro stimulation with vMIP and LPS, and discuss the effect of vMIP to cellular immunity. METHODS: The methods of Cross-linking of radioactivity, ELISA and four-colors flow cytometer were used to test the level of the secretion of chemokine IL-12 and intracellular cytokine IFN-? and IL-4. RESULTS: After treated the PBMCs with vMIP-II, the levels of secretion of IL-12, IFN-? and IL-4 were reduced in the present of LPS by competitively combining chemokine receptor; vMIP promoted CD4+T cell to secrete IL-12, IFN-? and IL-4. CONCLUSION: vMIP-II can protect systemic response of immunity and reduce extremely inflammation by down-regulating proinflammation.

Objective To analyze and evaluate the relationship between occupational lead exposure and spontaneous abortion.Methods The papers on the relationship of occupational exposure to lead and spontaneous abortion of female workers published in Chinese in 1991-2007 were searched by using computer and manual search,after strict selection,the homogeneity test and integrated analysis for the abstracted data of the eligible studies were conducted by using Rev.Man Statistic Software.The combined RR value was used as the total effect target of each project of meta-analysis.Results In total,14 original researches were included(totally 4 330 subjects and 11 970 controls),compared with the control,the occupational exposure to lead was significantly associated with spontaneous abortion(RR=2.83,95% CI:1.97-4.07)(P

Treating Hepatic Malignancies with radio- frequency is a new microinvasive method which has been developed in recent years. The patients treated with this method felt less painfull and recover quickly after operation. The author has reported the detailed observaticn to 39 patients with hepatic carcinomas and cooperated with the doctors for patients'psychological nursing in this article. During the treatment,the author tried to explain the aim,method and treating effect of the operation, help the patients to relieve nervousness end worries, observe the patients' inoperative response under cardiopneumograph and guide the patients for the postoperative observation of complications so that the treatment was completed successfully. The author suggests that sentimental support is an important step in the whole course of nursing

Objective To investigate the inhibitory effect of curcumin on the telomerase activity of HeLa cells.Methods The growth inhibition rate of HeLa cells treated with curcumin was measured with MTT method.The expression of hTERT mRNA was studied using RT-PCR.Telomerse activity was examined by TRAP-ELISA.Results The growth of HeLa cells treated with curcumin was inhibited in concentration dependent manner and the expression of hTERT mRNA and the level of telomerase activity were down-regulated significantly.Conclusion Curcumin can inhibit telomerase activity of HeLa cells.The underlying mechanism might be related to the down-regulation of hTERT transcription.

SDF-1α, a ligand for the chemokine receptor CXCR4, is well known for mediating the migration of breast cancer cells. In a previous study we demonstrated that a synthetic 21-mer peptide antagonist of CXCR4 (NT21MP) derived from the viral macrophage inflammatory protein II could antagonize tumor growth in vivo by inhibiting cellular proliferation and inducing apoptosis in breast cancer cells. However, the role of SDF-1α in the signaling pathways underlying the proliferation of human breast cancer cells and associated signaling pathways and inhibiting signal pathways of NT21MP remained unclear. The present study investigated the mechanism of NT21MP on anti-tumor in breast cancer in vitro. The effect of NT21MP on the viability of cells was determined by the MTT assay. Annexin V-FITC and PI staining was performed to detect early stage apoptosis in SKBR3 cells treated with SDF-1α and AMD3100 or NT21MP. Western blotting techniques were used to assay the composition of phosphoproteomics and total proteins present in the SKBR3 breast cancer cells. RT-PCR and Western blotting technique were used to detect the effect of NT21MP and AMD3100 on Bcl-2 and Bax expression. The results indicated that SDF-1α prevented apoptosis and promoted the proliferation of SKBR3 human breast cancer cells. As compared with untreated SKBR3 cells, Treatment with SDF-1α significantly increased cell viability, and NT21MP abolished the protective effects of SDF-1α dose-dependently (P<0.05). There was a significant decrease in the percentage of apoptotic cells after SDF-1α treatment as compared with control group (2.7%±0.2% vs. 5.7%±0.4%, P<0.05). But pretreatment of SKBR3 cells with NT21MP significantly attenuated the antiapoptotic effects of SDF-1α as compared with SKBR3 cells without NT21MP pretreatment. The proliferative and anti-apoptotic effects of SDF-1α in SKBR3 cells were associated with an increase in AKT and ERK1/2 phosphorylation as well as a decrease in Bax expression and an increase in Bcl-2 expression. These changes in intracellular processes were blocked by NT21MP in a dose-dependent manner(P<0.05). In conclusion, NT21MP efficiently inhibits SDF-1α-induced proliferation and antiapoptosis in SKBR3 cells by reducing the levels of phosphorylated AKT and ERK1/2, as well as decreasing the ratio of expression of Bcl-2 relative to Bax.

Aim Through interfering the expression of survivin with short hairpin RNA(shRNA) technology,to investigate the effect of downregulation of survivin on cell apoptosis and chemosensitivity to docetaxel in breast cancer MCF-7 cells.Methods(1) Using MCF-7 cells as a model system,three groups were set up transfected with lipofectamine,RNAi control plasmid and survivin RNAi plasmid,respectively.The expression of survivin in MCF-7 cells was measured at transcriptional and translational level by using RT-PCR and Western blot methods.(2) The effect on the cell cycle and apoptosis was analyzed with flow cytometry.(3) The viability of cells applied with different doses of ducetaxel was determined by using the method of 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide reduction(MTT method).Results(1) RT-PCR and Western blot demonstrated that survivin expression was significantly decreased by transfection with RNAi targeting plasmid;the expression proportion was reduced by nearly 75.4% and 79.8%(P

Objective　To probe the curative effect of thiotepa and mitomycin C for the prevention of the relapse of pterygium after its operation.Methods　One hundred and eighty-two cases with pterygium were randomly divided into two groups:one group of thiotepa(158 cases with 169 eyes)and the other group of mitomycin C (24 cases with 24 eyes) and a comparative study was conducted.Thiotepa was used through dropping on the third day after the operation(1∶2 000),while MMC was used in the same way during the operation and on the third day after the operation respectively(0.2g*L-1).Results　In the group of thiotepa,the wounded skin on the cornea was repaired significantly more rapidly than that in the group of mitomycin C,and the rate of the relapse of pterygium was lower in the former group than that in the latter one.Conclusion　Applying thiotepa by dropping after the excision of pterygium is a safe,simple and effective supplementary measure for the prevention of pterygium relapse and also effective for recurring cases.

Objective To discuss the ability of nursing major undergraduate students in disaster response in order to construct undergraduate's disaster response ability evaluation system.Methods Delphi method was used to draw the evaluation system framework on the basis of consulting literature material and analyzing theories.35 related experts attended it.The choice was evaluated according to the 5 classification Likert evaluation method.Results Consensus was reached after two rounds inst.Questionnaire recovery rate was 100％.Construct undergraduate's disaster response ability evaluation system with 3 first-level indexes,12 second-level indexes,37 third-level indexes.Conclusions The undergraduate's disaster response ability evaluation system based on Delphi experts method is reliable.It can provide reference for the nursing major undergraduate student's disaster response training and research.