0.05),but the relative levels of the mRNA of TNF-? and leptin were significantly down-regulated(P

0.05).TNF-? mRNA of intervenient group was significantly increased than that of control group(P

Objective To detect the intervened effect of the compound of Polygonum caspidatum to the NAFLD rat models and analyze its mechanism at the level of gene expression.Methods The Wistar rats were fed with high fatty chow to induce NAFLD and intervened with the compound of Polygonum caspidatum.The mRNA relative level of leptin,adiponectin,tumor necrosis factor ?,resistin and uncoupling protein 2 in the adipose tissue of the intervenient and control rats was detected with RT- qPCR method.The data differences between two groups were analyzed in t-test.Results Comparing to the control group,the relative level of leptin mRNA in the intervenient group was significantly increased(P0.05).At the same time,the resistin and UCP2 mRNA didn’t been detected in all samples of these two groups.Conclusion Metformin hydrychloride can adjust the adipose metabolism in liver and improve the steatosis and inflammatory reaction in liver cell.

0.05).TNF-? mRNA in the intervenient group was significantly decreased to that of the control group(P

Objective To develop a real-time electronic nursing records quality control system based on No.1 Military Medical Project to solve the existing problems in range,representativeness of detection results,information feedback,information storage and etc.Methods An integrated platform was implemented for information acquisition,integration and display on the bases of No.1 Military Medical Project as well as the interfaces with information management systems for electronic nursing records,clinical laboratory examination,medical examination,imaging and etc,which had the functions of auto monitoring of timeliness and logic of electronic nursing records,manual quality control of the connotation as well as the feedback,statistics and analysis on the problems.Results The system behaved well in stability,compatibility,and could be used for real-time and dynamic control of electronic nursing records,timely information feedback and statistical analysis.Conclusion The system contributes to the quality control of electronic nursing records,and is of great significance for its enhancement.

Objective To observe the application of amniotic membrane in conjunctival sac plasty.Methods 24 cases with different eye diseases were performed with conjunctival sac plasty,in which 11 eyes were performed with amniotic membrane transplantation,5 eyes with symblepharon which adhered with cornea or emerged neovascularization were performed with amniotic membrane transplantation and lamellar keratoplasty,other 8 eyes were performed with amniotic membrane transplantation and conjunctival fornixe.They were followed up for 6~12 months.Results There was no inflammation observed after operation.The transplanted amniotic membrane turned transparent and the conjunctiva at sewing location crawled to amnion 10 d after operation.One month later,the neonatal conjunctiva covered transplanting region completely.Among the 24 eyes,9 eyes were healed,13 eyes were improved,and 2 were ineffective.Conclusion Amniotic membrane is a kind of effective material during conjunctival sac plasty for narrow conjunctival sac.

AIM: To investigate the effect of nucleolin on diabetic cardiomyopathy in mice.METHODS: A type II diabetic cardiomyopathy mouse model was prepared using a cardiac-specific nucleolin-overexpressing transgenic mice.The mice were divided into wild-type mouse control group, nucleolin transgenic mouse control group, wild-type mouse diabetes group and nucleolin transgenic mouse diabetes group.Wheat germ agglutinin (WGA) fluorescent dye, Masson staining and PowerLab system detection were used to further clarify the role of nucleolin on cardiac hypertrophy, fibrosis and cardiac function in type II diabetic cardiomyopathy mice.RESULTS: Compared with wild-type mouse control group, no significant increase in blood glucose level was found, while genetical myocardial cell hypertrophy was significantly attenuated in nucleolin transgenic mouse diabetes group.The collagen fibers were also significantly reduced, and hemodynamic indexes ± dp/dtmax, left ventricular end-diastolic pressure, left ventricular systolic pressure and heart rate were also improved.The above differences were statistically significant.CONCLUSION: Nucleolin may reduce the occurrence of myocardial hypertrophy and fibrosis, thus improving the cardiac function of diabetic cardiomyopathy mice.

Objective To explore the application value of CEUS in diagnosis of renal occupying lesions.Methods Totally 67 patients with renal occupying lesions underwent conventional ultrasound and CEUS before surgical operation.CEUS enhancement characteristics were observed and compared between 40 patients with clear cell renal cell carcinoma (CCRCC)and 14 patients with angiomyolipoma (AML).Meanwhile the time-intensity curves were analyzed,including peak intensity (IMAX),rise time (RT),time-to-peak time (TTP) and mean transit time (mTT).Results There were significant differences in enhancement patterns,degrees,homogeneity of enhancement and pseudocapsule sign between CCRCC and AML (all P＜0.01).Compared with AML,IMAX of CCRCC was higher,RT and mTT were both earlier (P＜0.05).The sensitivity,specificity and accuracy of CEUS in diagnosis of malignant renal occupying lesions was 91.49 ％ (43/47),75.00 ％(15/20) and 86.57％ (58/67),respectively.Conclusion CEUS combined with quantitative analysis software is helpful to diagnosis and identification of CCRCC and AML.

Two new compounds (1 and 2), together with twenty-one known compounds (3-23), were isolated by a combination of various chromatographic techniques including column chromatography over macroporous resin, MCI gel, silica gel, and Sephadex LH-20 and reversed-phase HPLC. Their structures were identified by spectroscopic data analysis as 4-hydroxy-3-(4-hydroxybenzyl) benzyl methyl ether (1), 4-( methoxymethyl) phenyl-1-O-beta-D-glucopyranoside (2), hibicutaiwanin (3), 4-(4-hydroxybenzyl)-2-methoxyphenol (4), 4,4'-methylenebis(2-methoxyphenol) (5), L-phenyllactic acid (6) ,4-hydroxy-3-methoxybenzyl ethol ether (7), p-hydroxylbenzyl alcohol (8), p-hydroxylbenzyl methyl ether (9), p-hydroxylbenzyl ethyl ether (10), p-hydroxybenzaldehyde (11), p-hydroxybenzoic acid (12), p-hydroxybenzoic acid (13), gastrodin (14), 4-(ethoxymethyl) phenyl-1-O-beta-D-glucopyranoside (15), 4-(beta-D-glucopyranosyloxy) benzaldehyde (16), p-methylphenyl-1-O-beta-D-glucopyranoside (17 ), methyl-O-beta-D-glucopyranoside (18), 5-hydroxymethl-furan aldehyde (19), parishin (20), parishin B (21), parishin C (22), and diosgenin (23). The 13C-NMR data of compound 4 was first reported.
Drugs, Chinese Herbal ; chemistry ; Gastrodia ; chemistry ; Organic Chemicals ; analysis ; isolation & purification ; Water ; chemistry

This study explores the application effect of the case-based teaching method based on Xuexitong learning platform in the online teaching of Digestive System, and analyzes the learner's emotional experience, learning behavior, and learning effect in the case-based online teaching. The results of the study show that the case-based online teaching model based on Xuexitong learning platform improves students' online learning interest, and the students have good emotional experience, high learning enthusiasm, good classroom interaction, enhanced self-learning ability before and after class, and good learning effect. In addition, precise teaching can be used for individual students who are not enthusiastic about online learning.