Objective To investigate the clinical value of blood recovery in the heart valve replacement surgery of adults. Methods Selected 556 patients accepted cardiac valve replacement surgery from May 2008 to April 2012 in our hospital,and divid-ed into control group(278 cases)and observation group(278 cases)according to the way of intraoperative blood transfusion patients in observation group underwent autologous blood recovery and patients in control group underwent without autologous blood recov-ery,the clinical parameters were observed and statistically analyzed.Results The differences of blood routine after 24 h(such as he-moglobin,hematocrit,platelets,etc.)and blood gas analysis(such as pH,potassium ions,lactic acid,etc.)between two groups were not significantly different,which is statistically insignificant(P >0.05);the postoperative chest drainage of patients in observation group was not significantly different from that of patients in control group,which was statistically insignificant(P >0.05);the a-mount of banked blood in observation group(263.83 ± 19.23)mL was significantly lower than that in control group(615.24 ± 20.34)mL,the difference between two groups was statistically significant(P <0.05).Conclusion In heart valve replacement sur-gery of adults,blood recovery can effectively add blood volume,reduce autologous blood loss,and significantly reduce the amount of banked blood input,which is worthy of promotion.

The author discussed the course teaching reform of physical therapeutics of higher vocation community rehabilitation speciality from teaching form, textbook choice, teaching content, organizing theory and practice teaching, examination means, ect.

Kinesiology is an important special basic course of rehabilitation therapy specialty in the higher vocational college.The establishment of aim,class teaching content and outline,the preparation of teaching data,the innovation of teaching methods and measure,the reinforcement of practical teaching,and etc.,were discussed in the paper,and the consideration and experiences on the practical teaching reform and construction of kinesiology course in the higher vocational colleges were put forward.

Objective To investigate the protective mechanism of ischemic preconditioning for myocardial ischemic reperfusion injury (MI/RI) rats. Methods Eighteen male Sprague–Dawley (SD) rats were randomly separated into Sham operation,ischemic reperfusion (IR) and ischemic preconditioning (IP) groups (6 rats in each group). Peripheral blood and cardiac muscle were collected after the establishment of the above 3 animal models. ELISA was employed to determine proinflammatory cytokines TNF-? and IL-1? production in sera of these animals. RT-PCR and Western blot analysis was used to assay the transcriptional level and protein level of Toll-like receptor 4 (TLR4) in cardiac muscle tissue with pathological change,respectively. Results Compared with IR group,ischemic preconditioning effectively decreased the expression levels of TNF-? and IL-1? in sera of rats in IP group (P

ObjectiveTo investigate the effect of Bobath approach combined with acupuncture on spastic cerebral palsy. Methods30 children with cerebral palsy treated with acupuncture combined with Bobath approach were as study group, other 30 children treated with Bobath approach only as the control group. ResultsThere were 27 cases (93%) were effective in study group, and 21 cases (80%) in control. The scores of Gross Motor Function Measure (GMFM) improved in both groups (P＜0.05), but more in the study group (P＜0.05) after treatment. The spasticity alleviated in both study group (76.7%) and control group （50%）. ConclusionThe combination with acupuncture is more effective on spastic cerebral palsy than Bobath approach alone.

A registration form for orthopedics in-plant metal materials is designed to improve their management.The standardized operation process resulting from the registration form makes medical disputes due to quality problems of in-plant metal materials decreased greatly.Applied in 637 cases of orthopedics operations,the registration form is satisfactory.

Background Limbal stem cells (LSCs) play an important role on the stability of corneal epithelium and corneal transparency.Rho-associated coiled-coil containing protein kinase (ROCK) inhibitor can promote cell proliferation and reduce apoptosis,such as human embryonic stem cells and karatin epithelial cells.Objective This study was to investigate the improving effect of Y-27632,a ROCK inhibitor,on the activity of rabbit LSCs in corneal preservation medium.Methods Corneal preservation solution was prepared by adding 12.5％ chondroitin sulfate,10.0％ low molecular dextran,20.0 mg/L dexamethasone,100 mg/L tobramycin sulfate,9.5 g/L Hepes and 0.375 mg/L L-glutamine in MEM.The corneas of New Zealand white rabbits were collected and preserved in the corneal preservation solution with or without Y-27632 for 4,7,14 days,and the density and morphology of corneal endothelial cells were examined by using 0.25％ trypan blue staining and 0.2％ alizarin red staining.Isolated corneal epithelial cells were seeded on 3T3 feeder layer and cultured for 7-10 days until colonies formation.Colony shape of LSCs was observed under the light microscope,and colony-formation efficiency was analyzed after Giemsa staining by Image J software.Results The morphology and density of corneal endothelial cells were normal in the corneal preservation solution with and without Y-27632 for 4 days.In the seventh day after preservation,the cells remained the regular hexagon in shape in the preservation solution with Y-27632,however,the cellular membrane was slightly shrinking with the positive staining for alizarin red in the preservation solution without Y-27632.The density of corneal endothelial cells in the corneal preservation solution without Y-27632 was (2 262-± 75) cells /mm2,while in the preservation solution with Y-27632 was (2 425 ±95) cells/mm2(P＜0.001).The cloning spheres of LSCs were similar in preservation solution both with and without Y-27632 in the freshly isolated cornea or preserved corneas and exhibited more cells inside.But in 7 days and 14 days after preservation,the cloning spheres were much smaller in the preservation solution without Y-27632 group than those in the preservation with Y-27632 group.No significant differences were found in the cloning-formation rate and survival rate of corneal epithelial cells in corneas freshly isolated or preserved for 4 days in both groups (all at P＞0.05).In 7 days and 14 days after preservation,the active rates of corneal epitheli.al cells were (73.00±2.12)％ and (56.00±0.71)％ in the preservation solution with Y27632,which were significantly higher than (66.00 ± 4.00) ％ and (49.00 ± 0.71) ％ in the preservation solution without Y-27632,showing statistically significant differences between them (t =3.098,P =0.018;t =9.798,P =0.000).In addition,the cloning-formation rates of LSCs were (11.05±0.21)％ and (3.10±1.97)％ in the preservation solution with Y-27632 in 7 days and 14 days after preservation,revealing significantly elevation in comparison with (2.05 ± 1.20) ％ and (0.40 ±0.14) ％ in the preservation solution without Y-27632 (t =18.107,P =0.000;t=3.184,P=0.017).Conclusions Y-27632 promotes the vitality and cloning-formation ability of LSCs in corneal preservation medium,suggesting its potential use during storage of cornea.

The weakness of the boiler high pressure steam sterilizer is put forward.The development process and actual application value of the hot steam electrical generator is mainly introduced.

AIM: To investigate the protective effect of microRNA-218 (miR-218) silencing on kidney tissue of streptozotocin (STZ)-induced diabetic nephropathy rats and the potential mechanism.METHODS: The diabetic rat model was established by a single intraperitoneal injection of STZ (50 mg/kg).Meanwhile, the miR-218 short hairpin RNA (shRNA) lentiviral vector was constructed.The Sprague-Dawley rats were randomly divided into 4 groups: healthy control group, diabetes group, empty vector group and miR-218-shRNA group.The blood glucose, 24 h urinary protein, serum creatinine (SCr) and blood urea nitrogen (BUN) in the rats at different time points (4, 8 and 12 weeks) were measured by an automated analyzer.The expression of miR-218 was detected by RT-qPCR, while the expression of heme oxygenase-1 (HO-1), nephrin and p38 mitogen-activated protein kinase (p38 MAPK) at mRNA and protein levels in the kidney tissues was determined by RT-qPCR and Western blot.The caspase-3 activity was detected by caspase-3 activity assay kit, and the cell apoptosis of the kidney tissues was analyzed by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL).RESULTS: Compared with healthy control group, the expression of miR-218 was significantly increased in STZ-treated rats.Meanwhile, the concentrations of blood glucose, 24 h urinary protein, SCr and BUN were significantly increased in STZ-treated rats (P<0.05).The mRNA and protein expression of HO-1 and nephrin was significantly decreased, while the level of phosphorylated p38 MAPK was significantly increased in STZ-treated rats.In addition, the activity of caspase-3 was also significantly increased in STZ-treated rats.When the model rats were infected with miR-218-shRNA, the expression of miR-218 was significantly decreased and the above effects were markedly reversed.Furthermore, TUNEL results showed that compared with diabetic group and empty vector group, miR-218 silencing significantly attenuated the cell apoptosis in the kidney tissues in miR-218-shRNA group.CONCLUSION: miR-218 is involved in the kidney injury in diabetic rats, and silencing of miR-218 by lentiviral vector-mediated miR-218-shRNA transfection effectively inhibits kidney cell apoptosis, suggesting that miR-218 is a potential target for the treatment of diabetic nephropathy.

Objective To investigate the effect of cyclosporine A (CsA) on autophagylysosomal pathway in tubular epithelial cells.Methods Human renal tubular epithelial cell line (HK-2 cell) was treated with different concentrations (3,5 and 10 μmol/L) of CsA for 24 h.Then the viability and apoptosis of cells were measured by MTT assay or AnnexinV-PI staining followed by flow cytometry analysis,respectively.Autophagy-related protein LC3-Ⅱ and p62 were detected by immunofluorescence assay.Autophagic flux was analyzed in HK-2 cells transfected with a tandem mRFP-GFP fluorescent-tagged LC3 (ffLC3) plasmid by laser confocal microscope.The lysosomal degradation was evaluated by DQ-ovalbumin staining followed by flow cytometry analysis.Results The viability of HK-2 cells was significantly decreased with CsA stimulation when compared with control group (P ＜ 0.01),but the number of apoptotic cells was markedly increased by CsA treatment (P ＜ 0.05).Compared with the control group,different doses of CsA dramatically increased the expressions of LC3-Ⅱ (P ＜ 0.01) and p62 (P ＜ 0.05) in HK-2 cells.Moreover,HK-2 cells treated with CsA displayed a significant increase in autophagosomes but a marked decrease in autolysosomes.In HK-2 cells,exposured to CsA caused a decrease in lysosomal degradation by DQ-ovalbumin staining when compared with control group (P ＜ 0.01).Conclusion Blockade of autophagy via disrupting lysosome degradation may represent a novel mechanism of CsA-induced tubular epithelial cells injury.