OBJECTIVE:To investigate the therapeutic efficacy of edaravone combined with citicoline sodium on acute cere-bral infarction and its effects on the levels of oxidative stress and inflammatory factors. METHODS:108 patients with acute cere-bral infarction were randomly divided into edaravone group(single group)and edaravone+citicoline sodium group(drug combina-tion group),with 54 cases in each group. Based on routine treatment,single group was given Edaravone injection 30 mg added in-to 100 ml 0.9% Sodium chloride injection intravenously,bid,used up within 30 min each time;drug combination group was addi-tionally given Citicoline sodium injection 0.5 g added into 250 ml 0.9% Sodium chloride injection intravenously,qd,on the basis of single group. Treatment course of 2 groups lasted for 2 weeks. NIHSS,HDS,Barthel index,oxidant stress indicator and inflam-matory factors were compared between 2 groups before and after treatment. RESULTS:After treatment,the effective rate of NI-HSS in drug combination group was 81.48%,which was significantly higher than single group(53.70%),with statistical signifi-cance (χ2=9.511,P=0.002). HDS score and Barthel index of 2 groups were significantly increased after treatment,especially in drug combination group,with statistical significance(P<0.05). Compared with before treatment,contents of MDA and ET-1 in 2 groups were decreased significantly,while SOD activity and NO content were increased significantly;the inflammatory cytokines IL-6,IL-8,IL-12 and IL-16,TNF-α were all decreased gradually,with statistical significance (P<0.05);the improvement of each indicator in drug combination group was more significant than single group,with statistical significance(P<0.05). CONCLU-SIONS:Edaravone combined with citicoline sodium show good therapeutic efficacy in the treatment of acute cerebral infarction, can decrease the levels of oxidative stress and inflammation and promote the recovery of the neurological function and the daily liv-ing ability.

Escherichia coli (E. coli) FadR regulator plays dual roles in fatty acid metabolism, which not only represses the fatty acid degradation (fad) system, but also activates the unsaturated fatty acid synthesis pathway. Earlier structural and biochemical studies of FadR protein have provided insights into interplay between FadR protein with its DNA target and/or ligand, while the missing knowledge gap (esp. residues with indirect roles in DNA binding) remains unclear. Here we report this case through deep mapping of old E. coli fadR mutants accumulated. Molecular dissection of E. coli K113 strain, a fadR mutant that can grow on decanoic acid (C10) as sole carbon sources unexpectedly revealed a single point mutation of T178G in fadR locus (W60G in FadRk113). We also observed that a single genetically-recessive mutation of W60G in FadR regulatory protein can lead to loss of its DNA-binding activity, and thereby impair all the regulatory roles in fatty acid metabolisms. Structural analyses of FadR protein indicated that the hydrophobic interaction amongst the three amino acids (W60, F74 and W75) is critical for its DNA-binding ability by maintaining the configuration of its neighboring two β-sheets. Further site-directed mutagenesis analyses demonstrated that the FadR mutants (F74G and/or W75G) do not exhibit the detected DNA-binding activity, validating above structural reasoning.
3-Oxoacyl-(Acyl-Carrier-Protein) Synthase ; genetics ; metabolism ; Amino Acid Sequence ; Bacterial Proteins ; chemistry ; genetics ; metabolism ; DNA, Bacterial ; chemistry ; metabolism ; Escherichia coli ; genetics ; metabolism ; Escherichia coli Proteins ; genetics ; metabolism ; Fatty Acid Synthase, Type II ; genetics ; metabolism ; Fatty Acids ; metabolism ; Gene Expression Regulation, Bacterial ; Hydro-Lyases ; genetics ; metabolism ; Hydrophobic and Hydrophilic Interactions ; Lipid Metabolism ; Models, Molecular ; Molecular Sequence Data ; Mutation ; Protein Binding ; Protein Structure, Secondary ; Repressor Proteins ; chemistry ; genetics ; metabolism ; Sequence Alignment ; Sequence Homology, Amino Acid ; Signal Transduction

Objective:To explore the effects of "5E" rehabilitation nursing model in coronary disease patients after percutaneous coronary intervention (PCI) .Methods:We selected PCI patients of the Fourth Department of Cardiology in Affiliated Hospital of Jining Medical University as subjects by convenience sampling. A total of 82 patients from November 2018 to December 2018 were in control group, and 86 patients were from April 2019 to May 2019 were in observation group. Control group carried out the routine nursing model, while observation group implemented the "5E" rehabilitation nursing model. Self-management behavior and quality of life of patients between two groups were assessed with the Coronary Heart Disease Self-management Behavior Scale (CSMS) and the China Questionnaire of Quality of Life in Patients with Cardiovascular Diseases (CQQC) .Results:Three months after discharge, the sub-scale scores of disease medical management, daily life management in CSMS and the score of CQQC of patients in observation group were (45.77±6.39) , (34.28±5.83) and (71.23±18.53) respectively higher than those in control group with statistical differences ( t=2.813, 3.909, 2.301; P<0.05) . Conclusions:The "5E" rehabilitation nursing model for patients after PCI can improve patients' self-management behavior and quality of life.

Objective:To construct a key factor index system of health education for patients after percutaneous coronary intervention (PCI) based on behavior change wheel (BCW) theory.Methods:By adopting the convenient sampling method, a total of 33 nursing experts from 6 provinces and cities including Tianjin and Beijing were selected as objects for correspondence consultation in November 2019. With BCW as the research framework, the key factor index system of health education for patients after PCI was determined through literature search and 2 rounds of expert correspondence consultation.Results:The positive coefficients of two rounds of expert correspondence consultation were both 100%, the expert authority coefficients were respectively 0.858 and 0.881, and the coordination coefficients of expert opinions were respectively 0.336 and 0.387 ( P<0.01) . The final established key factor index system of health education for patients after PCI consisted of 3 first-level indexes, 7 second-level indexes and 43 third-level indexes. Conclusions:The key factor index system of health education for patients after PCI constructed in this study is a set of scientific, reliable and credible index system, which can provide a theoretical basis for formulating intervention measures for patients after PCI.