Objective To investigate the status of TCM development in Chongqing city,and to put forward countermeasures and suggestions of the development of Chinese medicine in view of the existing problems,so as to provide reference for the government and relevant departments.Methods The relevant questionnaires were issued and recycled in 20 districts and counties of Chongqing city.Part of the data culled from Chinese management office yearbooks of Chongqing.Finally,comprehensive analysis was per-formed.Results There′s enormous upside potential for the development of Chongqing traditional Chinese medicine (TCM),but there were some problems and deficiencies.The main problems were as follows:the TCM characteristics not brought into full play in traditional Chinese Medicine hospitals,lack of following talents,unsound organization management of traditional Chinese medi-cine,the government′s insufficient investment and attention for traditional Chinese medicine (hospitals,schools,“hand-to-hand”ap-proach),not enough national policy support for TCM,TCM hospitals and folk medicines of ethnic minorities,and insufficient exca-vation in ethnic minority medicine.Conclusion In order to adapt to the development of Chinese medicine and to meet the growing requirements of Chinese medicine,some countermeasures should be taken as follows:giving full play to the characteristics of tradi-tional Chinese medicine,strengthening personnel training in Chinese medicine,reforming the management mechanism,increasing the government investment,strengthening policy support for Chinese medicine and folk medicines of ethnic minorities,accelerating the process of TCM standardization,scientific and internationalization.It is suggested that the miscellaneous diseases &incurable dis-ease medicine research office and slow disease traditional Chinese medicine health care office should be established under the TCM management office.Specialized agencies are advised to mine and collect herbal remedies,recipes in ethnic minority area and so on.

Objective To evaluate prognostic value of N-terminal B-type brain natriuretic peptide (NT-proBNP) combined with galectin 3 of prognostic value in patients with chronic heart failure (CHF).Methods Determination of 92 cases of CHF patients on admission and at discharge plasma NT-proBNP and galectin-3 levels,then after the patients discharge we follow up 16 months and observe the internal recurrent heart failure hospitalization rate and mortality.Results In patients the heart function of New York Heart Association (NYHA) Ⅰ,Ⅱ,Ⅲ,Ⅳ level,NT-proBNP were (351.6 ± 467.5) pg/ml,(493.2 ± 411.7) pg/ml,(1 289.6 ± 737.8) pg/ml,(2 719.2 ± 1 044.1) pg/ml,galectin-3 (12.23 ± 6.12) ng/ml,(13.59± 5.46) ng/ml,(19.18 ± 7.44) ng/ml,(26.61 ± 8.82) ng/ml as well.NT-proBNP and galectin-3 levels were increased with the depression of cardiac function.The patients who had higher NT-proBNP or galectin3 levels discharged and occurred the more probability of re-hospitalization on account of heart failure or death.Compared to those patients with one of them increased,the patients whose discharge NT-proBNP and galectin-3 value were both increased with significantly poor outcome.Conclusions Both NT-proBNP and galectin-3 were independent predictors of poor prognosis in patients with heart failure.Galectin-3 combined with NT-proBNP can assess the prognosis of patients with CHF more effectively.

Objective　To explore the prophylactic effect of T cell vaccination (TCV) on type 1 diabetes. Methods　6-week-old nondiabetic femele NOD mice were vaccinated intraperitoneally with the attenuated, activated T lymphocytes isolated from the spleen of 4-week-old nondiabetic NOD mice, 18-week-old newly diabetic NOD mice and 32-week-old long-term diabetic NOD mice. The incidence of cyclophosphamide-treated diabetes, inflammatory score of insulitis in NOD mice as well as T lymphocyte subset changes in spleen and thymus were determined after TCV. Results　TCV was able to reduce the incidence of cyclophosphamide-treated diabetes, to alleviate insulitis, to increase the percentage of CD8+ T lymphocyte subset in spleen and CD4- CD8+ simple-positive T lymphocyte in thymus, and to decrease the percentage of IL-2R+ T lymphocyte and CD4+/CD8+ rate in spleen. Conclusion　TCV may decrease host autoimmunity, which seems related to the changes of T lymphocyte subsets in spleen and thymus and prophylactic effect on diabetes.

BACKGROUND: The relationship of trace element and human body health is increasingly paid more attention. Many trace elements are indispensable for organism. The lack of trace element is closely related to the occurrence of cardio-cerebrovascular disease, while the medicative effect of traditional Chinese medicine depends on the sorts and level of the contained trace elements.OBJECTIVE: To determine the level of Ca, Mg, Cu, Cr, Zn and Fe in 10 traditional Chinese medicine patent prescriptions for the treatment of cerebrovascular disease.SETTING: Medical Experimental Center of Jilin Medical College.MATERIALS: This experiment was carried out in the Jilin Institute of Chemical Technology during March to May 2003. Ten traditional Chinese medicine patent prescriptions Naosaitong, Qiangli Tianma Duzhong Wan,Kangshuan Wan, Tongmai Ningxin Chongji, Rongshuan Jiaonang,Buchang Naoxintong, Dahuoluodan, Zhongfeng Huichun Wan, Xiaoshuan Tongluo Jioonang and Huatuo ZaizaoWan, purchased from market were selected for the treatment of cardio-cerebrovascular disease in this study.Strong HNO3 and grade-guaranteed reagent were made in Beijing Chemical Co. Ltd; HC1O4 and another kind of grade-guaranteed reagent were made in Tianjin Dongfang Chemical Co. Ltd.METHODS: Ten pills and 10 tablets were chosen and crushed evenly respectively. Two portions were equally chosen from each sample, 1.00 geach portion, and placed in ten 50 mL conical flask respectively. Then,strong HNO3 of 10 mL was used to envelop the conical flask, overnight. On the next day, strong HNO3 of 10 mL and HClO4 of 5 mL were respectively added into each flask, then which was slowly heated on the electric hot plate. When about 2 mL liquid was left in the conical flask, another 20 mL strong HNO3 and 5 mL HC1O4 were respectively added to further digest till the solution was achromatic and transparent, and flask was nearly dried.After cooled, the samples were transferred to a 25 mL volumetric flask and metered with 3% HNO3. The level of Ca, Mg, Cu, Cr, Zn and Fe 6 metallic elements in10 traditional Chinese medicine patent prescriptions was measured respectively with atomic absorption spectrophotometry.MAIN OUTCOME MEASURES: The level of Ca, Mg, Cu, Cr, Zn and Fe 6 metallic elements in 10 traditional Chinese medicine patent prescriptions.RESULTS: Among the levels of metallic elements in 10 traditional Chinese medicine patent prescriptions, the level of Mg was found the most in Rongshuan Jiaonang [(89.201 6±0.855 4) mg/g]; The level of Ca the most in Dahuoluodan [(14.312 5±0.507 0) mg/g]; Zn and Fe the most abundant in Kangshuan Wan [(60.487 5±1.639 8)mg/g and(980.081 2±15.395 9) mg/g respectively]; while the level of Cr very more in the Xiaoshuan Tongluo Jiaonang [(9.881 2±0.734 4) mg/g].CONCLUSION: Six metallic elements are all abundant in 10 traditional Chinese medicine patent prescriptions, but significant differences exist.Experimental results provide some evidences for investigating the relationship of the levels of metallic elements in 10 traditional Chinese medicine patent prescriptions and the therapeutic effect on treating cerebrovascular disease.

Pituitary stalk interruption syndrome(PSIS) is a rare disease characterized by different degrees of anterior pituitary hormone deficiency.The typical imaging finding is a thin or absent pituitary stalk,often associated with an ectopic posterior pituitary and a hypoplastic anterior pituitary.The cause is still unkown.Dynamic contrastenhanced MRI helps to confirm the diagnosis.Replacement of multiple anterior pituitary hormones is the key of the treatment.Doctors should have a thorough understanding of the disease and give patients efficacious treatment and a better prognosis would be obtained.

Objective To compare the clinical effect and safety evaluation of three different dose regimens for treating children with viral encephalitis.Methods Totally 126 cases treated in Xi'an Central Hospital from January 2010 to December 2015 were randomly divided into observation group 1 (ganciclovir combined with gangliosides,42 cases),observation group 2 (ganciclovir combined with gamma globulin,43 cases),and control group (39 cases).The clinical effect and levels of NSE,inflammatory cytokine were compared in the three groups.Results The total effective rate in observation group 1 was 95.24％ and that of observation group 2 was 93.02％,which were significantly higher than that of control group (79.48％).The disappearance time of headache,fever,convulsions,clouding of consciousness,meningeal irritation sign,cerebrospinal fluid abnormalities,and length of stay in observation groups (both 1 and 2)were significantly shorter than those in control group (P ＜ 0.05);After therapy,the levels of NSE in three groups were obviously decreased compared with those before therapy (P ＜ 0.05),and those in observation group were significantly lower than the control group (P ＜ 0.05);the levels of inflammatory cytokine in all three groups were obviously decreased compared with those before therapy (P ＜ 0.05),and that of observation group 1 had no statistical difference with the normal group,whereas that in control group was significantly higher than the normal group (P ＜ 0.05).Conclusion Ganciclovir combined with gangliosides as well as ganciclovir combined with gamma globulin were both effective methods in treating children with viral encephalitis and could decrease levels of inflammatory cytokine.Ganciclovir combined with gangliosides could effectively repair nerve damage,which deserves clinical expansion.

Background and purpose:The development of gastric cancer has a long course from chronic mucosal inflammation and involves a lot of genes.We investigated the different gene expression profiles with gene chip of high flux between gastric cancer and chronic atrophic gastritis so as to analyze their biologic significance,combined with endoscopic findings and surgical pathology.Methods:Paired pathological and non-pathological specimens from 23 patients with gastric cancer and 15 with chronic atrophic gastritis were enrolled for the study.Gene chip with 13 824 genes/ESTs was employed for detection of the expression profile,while endoscopic and pathologic findings were collected for evaluation.Data were analyzed by average-linkage hierarchical clustering,t test and Fisher calculation of exact probability.Results:Fifty-three specific genes were filtered from gastric cancer group,and 21 from chronic atrophic gastritis group.In the former group,STS,HAP1 and some other relatively specific genes were expressed in correlation to the Borrmann's classification,while in the latter group,BRD3 and BF508879 were demonstrated positively in correlation to intestinal metaplasia.However,there were also some specific genes,such as SLC26A3,related to precancerous condition,commonly expressed in both two groups.Conclusions:There was either homogenous or heterogeneous gene expression profile in gastric cancer as well as in chronic atrophic gastritis.Abnormal gene expressions were related to precancerous conditions or precancerous lesions and to biologic behavior of tumor.The difference and comparability of gene expression in the two groups studied shows the continuity and pertinence of the two diseases.

Objective To investigate the clinical efficacy and side reaction of tandospimne citrate capsules on treating patients with depression after stroke.Methods Seventy-eight patients who were diagnosed as anxiety and depression after stroke were taken as our subjects.They were randomly divided into control group (n =40) and treatment group (n =38).Patients in the control group were given conventional therapy for cerebral vascular disease and psychological treatment,while in the treatment group were given the same treatment besides tandospimne citrate capsules of 10 mg per times,3 times/d oral,and course of both two groups for 4 weeks.The patients of two groups were scored according to Hamilton Depression Scale(HAMD) at the time of pre-treatment and post-treatment.And the the clinical efficacy and side reaction were observed and compared.Results (1) HAMD scores of treatment group in 2,4 weeks after treatment had significantly lower than that before treatment ((8.09±2.21) vs.(6.31 ±2.28) vs.(11.98 ±2.17),P ＜0.05).HAMD scores of control group in the treatment at 2week was as same as that in 4 weeks((10.57 ±2.53) vs.(8.91 ±2.31)).There was statistically significant difference in two groups in 2,4 weeks after treatment in terms of HAMD scores(P ＞0.05).The total effective rate of control group was 31.6％ (12/38),and 92.5％ (37/40) in treatment group,and the difference was significant(x2 =63.69,P ＜ 0.01).In the course of treatment in the two groups,there were no obvious adverse reactions.Conclusion Tandospirone citrate capsules show the clinical curative effect in the treatment of anxiety and depression after stroke,and no obvious adverse reactions.

Objective To explore the effect of predictively nursing care for children with congenital large-sized nevus.Methods 142 cases of children with congenital large-sized nevus were treated by surgical resection,based on the principle of voluntary and informed patients,and order of children admitted to hospital were randomly divided into control group and observation group,and the two groups of children were 71 cases.Children in the control group received routine care;children in the observation group received nursing care support.Comparison of the two groups of children with treatment compliance and the satisfaction of paediatric patients' family,postoperative recovery and complicatians were observed.Results Observation group of patients treatment compliance was significantly higher;the difference was significant (P＜0.05).Observation group of children family satisfaction was significantly higher [97.2 ％ (63/71) vs 88.7 ％ (63/71)],the difference was significant (P ＜ 0.05).1,3,6,and 9-month postoperative follow-up was observed in children with recovery of the observation group were better than the control group;the difference was significant (P＜0.05).After nine months the two groups of children were cured.Group of children were observed without serious complications;redness developed in 1 patient in the control group and skin graft necrosis developed in 2 patients.Conclusions Pediatric patients with congenital large-sized nevus with predictively nursing care measures have better clinical efficacy,worthy of promoting.