[Objective]To measure the contents of rhychophyline in uncaria,and its regularity of variety with decoction time.[Methods]RP-HPLC method was used.The chromatographic conditions were listed below:Hypersil-BDS C18 column(150mm?4.6mm.5?m),amobine phase composed of metranol-water(70∶30),a flow rate of 1ml /min and wave lengths was 254nm,column temperature was 25 ℃.[Results]In the range of 0.19?g ~0.95?g,the linear was very well between the content of rhychophyline and peak area.[Conclusion]The contents of rhychophyline in decoction reached the peak at 11.4min after uncaria was added in boiling water.

Hepatitis C virus (HCV)-related thyroid dysfunction is a common complication in patients with chronic hepatitis C,including Graves' disease,Hashimoto thyroiditis,destructive subacute thyroiditis,hyperparathyroidism or hypothyroidism,with or without abnormal autoantibodies or thyroidrelated antibodies.The possible pathogenesis of HCV-related thyroid dysfunction includes bystander activation,molecular mimicry,effects of interferon and susceptibility associated with genetic polymorphism on particular locus.By now,there is still no criteria for diagnosis and treatment of HCV-related thyroid dysfunction.For patients with mild HCV-related thyroid dysfunction,observation may be appropriate; and for those with serious symptoms,anti-thyroid drug,levothyrocine,surgery or symptomatic treatment may be applied according to clinical symptoms.This paper gives an overview of the prevalence,clinical characteristics and possible pathogenesis of HCV-related thyroid dysfunction.

Objective To analyze the clinicopathologic features,diagnosis and different diagnosis of solid papillary carcinoma (SPC) of the breast.Methods The clinical data and pathological section,including HE and immunohistochemical staining slice,of 12 cases of SPC in People's Hospital of Shanxi Province from December 2008 to August 2014 were analyzed.Simultaneously,a literature search was conducted by utilizing Pub Med for all reported cases in English from November 1995 to October 2014.Results All cases were female,aged from 50 to 77 years old,manifested as mass or nipple discharge.Histologically,all cases appeared as multiple,well-circumscribed nodules filled by monotonous,low-moderate grade cell separated by fibrovascular cores.Immunohistochemically,CgA,Syn,ER and PR were positive in 12,11,9 and 5 cases,respectively.Her-2 presented as 1+ in 1 case (1/12) with invasive mucinous carcinoma,and locally poor positive in 2 cases without invasive component,while CK5/6 was negative in all cases.p63 and CK5/6 were positive in 6 and 5 cases at the location of axis of papillary,respectively.Meanwhile,8 and 7 cases at the border of duct,respectively.Conclusion SPC is an uncommon breast tumor,which presents with specific clinicopathologic features.

Objective To explore the factors associated with quality of life(QOL)in patients with chronic obstructive pulmonary diseases(COPD).Methods Questionnaire was used to investigate 206 COPD patients,including demographic information,disease-related knowledge-attitude-practice and QOL scale.Results The total score of QOL in these patients was(64.70?14.78).There was statistically significant correlation between the quality of life and frequency of hospitalization in the past 2 years,marriage,education,course of disease by stepwise multiple regression analysis.Conclusion The quality of life in COPD patients is poor,especially in those who are spouseless,of longer disease course,of lower education degree,frequently hospitalized and insufficient for healthy practice.

Dietary survey, physical examination and blood tests were carried out on kindergarten children from two to seven-year old in Wuhan, excluding some influencing factors such as malnutrition and so on. Three groups, 50 children each, were differentiated according to serum vitamin A level .avita-minotic (011 mmol/L). Immunological parameters were determinated before and after vitamin A supplementation. The results showed.'there were significant differences of S-IgA,C3 and lymphocyte transformation active response to PHA and lysozyme between vitamin A deficiency and normal group, but not on IgG, IgA, IgM and PHA skin test. After vitamin A supplementation these immunological functions could be improved. So we concluded vitamin A in subclinic deficiency could influence immune function too, but it could be recovered through vitamin A supplementation.

Objective To investigate the effect of urotensin Ⅱ on myocardial fibrosis in rats.Methods The pressure overload animal model was established in rats by abdominal aorta coarctation.The rats were divided into sham operation group,modeled for 4,8 and 12 weeks group.The expression of U Ⅱ,GPR14,col-Ⅰ,col-Ⅲ,and PKA in cardiac tissues was detected by Western blot.Isolated and cultured cardiac myofibroblasts (CFs) from new-born SD rats were treated with U Ⅱ,KT5720 or SB-611812,and then the proliferation of CFs was observed by micro scope and CKK-8.Results The expression of U Ⅱ,GPR14,col-Ⅰ,col-Ⅲand PKA increased markedly in cardiac tissues of model rat,which were time-dependent.U Ⅱ promoted the proliferation of CFs (P＜0.05),which could be inhibited by KT5720 or SB-611812.Conclusions U Ⅱ/UT system promotes the occurring and development of myocardial fibrosis.

AIM: To explore the protein levels of chemokine receptor 7 (CCR7) and vascular endothelial growth factor ( VEGF)-C in breast carcinoma , and to investigate the effects of CCR 7 and VEGF-C on prognosis of breast carcinoma.METHODS:The protein expression levels of CCR 7 and VEGF-C in the breast carcinoma tissues and normal breast tissues were detected by the method of immunohistochemistry .At the same time, the relationship between clinico-pathologic characteristics and the protein expression of CCR 7 and VEGF-C in the breast carcinoma tissues was analyzed . The relationship between the protein expression of CCR 7 and VEGF-C and survival time of the breast cancer patients was estimated by Kaplan-Meier method.RESULTS:The positive expression rates of CCR 7 and VEGF-C in the breast carcino-ma tissues were significantly higher than those in the normal breast tissues (P<0.01).A positive correlation was observed between the protein expression of CCR7 and the protein expression of VEGF-C in the breast carcinoma tissues (r=0.613, P<0.01).The protein expression of CCR7 and VEGF-C was correlated with lymph node metastasis and TNM stage (P<0.05), but both were not related to patients ’ age, primary tumor size, estrogen receptor and progesterone receptor .The survival time of the patients with CCR 7 and VEGF-C positive expression was significantly shorter than that of the patients without the expression (P<0.05).CONCLUSION:The positive expression of CCR7 and VEGF-C proteins is associated with the prognosis of breast cancer , and combined detection of CCR 7 and VEGF-C protein expression levels may be helpful to judge the prognosis of breast cancer .

Objective To guide clinical operation,the invasion,efficacy,convalescence by different operating ways on cardiac surgery were studied.Methods Left axillary minithoracotomy was applied to 41 patients with ductus atteriosus(A group);right axillary minithoracotomy was applied to 46 patients with atrial soptal defect (ASD),ventricular septal dofect(VSD),triple-symptom complex of Fallot(C group);49 patients with replacement of valvular heart,VSD,ASD,totralogy of Fallot,left atrial myxoma were operated in substernal segment minithoracotomy (D group).The comparison between above groups and the regular left chest posterolateral operation on 42 patients (B group) and the sternal median operation on 77 patients (E group)was carried out.Results (1) A group had such advantages as the operation time,hemorrhage volume,hospital day,compared with B group [(38±13) min vs (64±14) min,(17±12) ml vs (200±100) ml,(6±2) d vs(11±3) d,respectively](P<0.01).(2) There were difference in extra corporeal circulation time,bemorrhage volume and hospital day between C group and E group[(39±8) min vs (68±8)min,(150±150) ml vs (700±300)ml,(8±3)d vs(12±4)d,respectively](P<0.01 or <0.05).There were difference in hemorrhage volume,fluence between D group and E group (P<0.05).Conclusion Small incision,slight trauma,less hemorrhage,slight ache,quick recovery,concealed incision and so on are characteristic of the left subaxillary minithoracotomy for ligation of ductus arteriosus,the right subexillary minithoracotomy for opening heart operation with heart beating and the substernal segment minithoracotomy for opening heart operation with extra corporeal circulation.

Objective To investigate the effects of health education on enhancing the compliance behavior of patients with diabetes mellitus (DM). Methods 63 DM patients were provided with systemic health education during their staying in the hospital. A follow-up interview was performed at the 6th month of discharge to gather the information of how importance of compliance behavior on the prognosis of DM. Result After the systemic health education, the patients could take medicine on time and had a good behavior in daily life, which will effectively improved their quality in the future life.Conclusion Systemic the health education can improve the compliance behavior of patients, and thus directly affects the prognosis of DM.

Objective To investigate the effect of cancer drug sensitivity on the selection of chemotherapy for pancreatic cancer. Methods The cells from the cancer tissues of 156 pancreatic cancer patients were cultured with 6 kinds of chemotherapy drugs in vitro including gemcitabine (GEM), 5 fluorouracil (5-FU), Mitomycin C (MMC), Oxaliplatin (L-OHP) and irrinotecan (CPT-11), according to the in vitro standard of solitary tumor. More than 70% of inhibitory rate was highly sensitive, 50% ～ 70% was moderately sensitive, ＜ 50% was insensitive. The inhibitory rate of chemotherapy drugs were determined by MTT colorimetric assay. Results The pathological findings of the 156 cases of pancreatic cancer were pancreatic duct cancer in 135 cases, adenosquamous carcinoma in 13 cases, mucinous carcinoma in 8 cases.Pancreatic duct cancer was sensitive to all the 6 drugs; adenosquamous carcinoma and mucinous carcinoma was sensitive to all the drugs except for L-OHP and CPT-11, respectively. The inhibitory rate of GEM was higher than that of MMC, L-OHP and CPT-11 (P ＜ 0.05 ) , but there was no difference with 5-FU and DDP ( P ＞0.05 ). There was no difference among the other 5 drugs (P ＞ 0.05 ). However, the cells from different types of pancreatic cancers and the cells from different patients of the same type of pancreatic cancer have different sequence of sensitivity to the 6 kinds of chemotherapy drugs. For pancreatic duct cancer, the sequence of sensitivity was GEM ＞ DDP ＞ 5-FU ＞ CPT-11 ＞ MMC ＞ L-OHP; adenosquamous carcinoma was GEM, CPT-11 ＞ DDP, 5-FU, MMC ＞ L-OHP; mucinous carcinoma was L-OHP ＞ GEM ＞5-FU, MMC ＞ DDP ＞ CPT-11.Conclusions Cancer drug sensitivity test may help to select the fight chemotherapy and be of clinical value.