Objective To investigate the mixed model in bier-archical classification datas and implementing with mixed model in SAS. Methods Hierarchical classification datas exemplify the mixed model u-sing procedure mixed,and compared with traditional general linear model. Results The example shows the same result between the SAS mixed model and the general linear model. Conclusion SAS MIXED can flexi-bly fit and analysis hieraxchical classification datas.

Objective: To examine the effect of lifestyle behaviors on myopia among primary and middle school students in Yinzhou District, Ningbo City using latent class analysis, so as to provide insights into prevention of myopia among primary and middle school students. Methods: A total of 1 547 students were sampled from primary and middle schools in Yinzhou District using a stratified cluster sampling method. Myopia-associated lifestyle behaviors were collected using questionnaires, and visual acuity was tested. Myopia-associated lifestyle behaviors were subjected to latent class analysis among primary and middle school students, and the association between lifestyle behaviors and risk of myopia was examined using a logistic regression model. Results: A total of 1 547 questionnaires were allocated, and 1 494 valid questionnaires were recovered, with an effective recovery rate of 96.57%. There were 247 primary school students (16.53%), 452 junior high school students (30.25%), 532 senior high school students (35.61%) and 263 vocational high school students (17.60%), and 773 men (51.74%) and 721 women (48.26%). Latent class analysis showed that students' lifestyle behaviors were classified into three groups, including the healthy behavior group (37.68%), reading and writing fatigue group (42.64%), and video fatigue and poor diet group (19.68%), with myopia prevalence of 79.22%, 88.38% and 86.73%, respectively. Moderate myopia was predominant in the reading and writing fatigue group and video fatigue and poor diet group, and low myopia was predominant in the healthy behavior group. A higher risk of myopia was found in the reading and writing fatigue group (OR=1.996, 95%CI: 1.454-2.739) and video fatigue and poor diet group (OR=1.715, 95%CI: 1.157-2.543) than in the healthy behavior group. Conclusions Long reading and writing duration, insufficient exercise and sleep, long video watching duration, and high intake frequency of sugary drinks and fried foods may increase the risk of myopia in primary and middle school students in Yinzhou District. Targeted myopia interventions are required tailored to different categories of lifestyle behaviors.

Objective To investigate the effects of myricetin (Myr) on A549 lung cancer cell proliferation and its mechanism. Method The inhibitory effects of Myr on the growth of A549 cells were investigated and its value of IC50 after 72 h incubation was measured by 3-(4,5-dimethyl-thiazolyl -2)-2,5-diphenyl tetrazolium bromide (MTT) method. The phosphorylation level of Akt protein was studied by Western blotting. Results Myr inbibited A549 cell proliferation in dose-dependent way. The IC50 value of Myr treatment for 72 h was 41.7 ?g/ml. Treatments with 32 ?g/ml Myr for 12 h significantly reduced the protein kinase B (Akt Ser473) phosphorylation. Conclusion Myr could inhibit the growth of A549 cell in a dose-dependent way. Akt phosphorylation may be the mainly molecular target of Myr in A549.

0.05) at 8 w. At 8 w body fat and TG in groups B and C were significantly lower than group A (P

Objective To evaluate the feasibility of 80 kV tube kilovoltage in aortic artery CT angiography with 256-slice CT. Methods A total of 62 patients undergoing aortic artery CTA were enrolled into this study and divided into conventional tube kilovoltage (120 kV, n = 31 ) and low tube kilovoltage (80 kV, n = 31 ). The signal-to-noise ratio (SNR) , contrast-to-noise ratio (CNR), volume CT dose index (CTDIvol) and effective dose (E) were evaluated, respectively. Results The mean image SNR was ( 35.92 ± 5.04) and ( 33.95 ± 8. 30) for conventional tube kilovoltage and low tube kilovoltage,respectively, with no significant difference (t = 1. 131, P =0. 263). The mean image CNR was (30. 32 ±4.78) and (28.71 ± 7.96 ) for conventional tube kilovoltage and low tube kilovoltage, respectively, with no significant difference ( t = 0. 964, P = 0. 339 ). The average effective dose ( E ) was ( 14. 28 ± 0.96 )mSv and (9. 72± 0. 81 )mSv for conventional tube kilovoltage and low tube kilovoltage, respectively, with significant difference ( t = 20. 12, P ＜ 0. 001 ). Conclusions 80 kV tube kilovoltage aortic artery CTA can reduce radiation dose by 31.9% , and contrast dose 50% ,and maintain image quality compared with 120 kV tube kilovoltage.

Objective To evaluate the feasibility of automatic tube current modulation(ATCM) in craniocervical artery CT angiography with 256-slice CT,while maintaining the image quality.Methods Sixty patients undergoing craniocervical artery CTA were enrolled into this study,and were divided into conventional tube current group(250 mAs,group A,n=30) and low tube current group(ATCM,group B,n=30).The signal-to-noise ratio(SNR),contrast-to noise ratio(CNR),volume CT dose index(CTDIvol) and effective dose(ED) were evaluated respectively.Results The mean image SNR was(37.47?9.39) and(36.85?7.96) for group A and group B respectively,and was not significantly different(P=0.79).The mean image CNR was(32.13?9.06) and(31.86?7.66) for group A and group B respectively,and was not significantly different(P=0.90).The CT volume dose index(CTDI) was 16.5 mGy and 410.55?0.94mGy for group A and group B respectively,and was significantly different(P

Objective To determine the efficacy of lifestyle interventions in adults with impaired glucose tolerance (IGT).Methods MEDLINE (Pub Med),EMBASE,Science Citation Index (SCI),and Cochrane Database were retrieved for articles about the relations of lifestyle intervention and IGT.Searches were limited to English language publications.The RCTs outcome evaluated in this study included 2 h plasma glucose level and fasting plasma glucose,meta-analysis was carried oat by Stata 11.0 among articles for the inclusion and exclusion criteria.The difference of effects was expressed as standardized mean deviation(SMD).Results A total of 10 RCTs studies met the inclusion criteria.Lifestyle interventions were associated with a decline in 2 h plasma glucose and fasting plasma glucose level (SMD =-0.67,P＜0.01 ;SMD =-0.33,P＜0.01),but high heterogeneity was identified in this meta-analysis.Conclusion Physical,dietary and both combined interventions can reduce 2-h plasma glucose and fasting plasma glucose levels in adults with impaired glucose tolerance.As high heterogeneity was identified in this meta-analysis,more high quality research is needed.

Objective: To analyze the association between latent class of health-risk behaviors and depressive symptoms among middle school students, so as to provide the evidence for the prevention and intervention of depressive symptoms among middle school students. Methods: Students in two junior high schools, two senior high schools and one vocational high school in Yinzhou District, Ningbo City, Zhejiang Province, were selected using a stratified multi-stage cluster sampling method. Demography and health-risk behaviors were collected using questionnaire surveys, depressive symptoms were investigated using the Center for Epidemiological Studies Depression-10 Scale, and latent class analysis was conducted for health-risk behaviors. The association between different latent classes and depressive symptoms was analyzed using a multivariable logistic regression model. Results: A total of 1 247 students were surveyed, including 641 boys (51.40%) and 606 girls (48.60%). There were 452 junior high school students (36.25%), 532 high school students (42.66%) and 263 vocational high school students (21.09%). Latent class analysis showed that health-risk behaviors in students were classified into three groups, namely healthy behavior group (52.93%), poor diet group (39.94%) and high-risk behavior group (7.14%), and the detection rates of depressive symptoms were 7.12%, 18.88% and 52.81%, respectively, with a statistically significant difference between groups (P<0.05). Multivariable logistic regression analysis showed that after adjusting for age, gender, native place, only child and living on campus, the poor diet group (OR=3.107, 95%CI: 2.086-4.627) and high-risk behavior group (OR=15.401, 95%CI: 9.031-26.262) had higher risks of depressive symptoms compared with the healthy behavior group. Conclusion Having high-risk behaviors and poor diet may increase the risk of developing depressive symptoms among middle school students.

Objective: Blue dye was one of the methods for sentinel lymph node biopsy in breast cancer, but the success rate was affected by multiple factors. This study was to determine the related factors contributing to the success of sentinel lymph node biopsy in breast cancer using blue dye method. Method:From Jan. 2007 to Aug.2008, sentinel lymph node biopsy was performed by periareolarly injecting 1% methylene blue in 141 breast cancer patients followed by axillary clearance. Pathological diagnosis was performed for all of the lymph nodes by conventional HE staining. Unconditional logistic regression was applied to make both univariate and multiva-riate analysis. Results:Sentinel lymph node was successfully detected in 126 patients. But the biopsy was failed in the 15 patients. The success rate was 89.4% and the false negative rate was 6.82%. Univariate analysis showed that success rate of biopsy was in association with patients' age, body mass index (BMI), tumor size, preoperative axillary node's status, pathological grade as well as ≥4 positive axillary lymph nodes involved. Multivariate analysis indicated that the patients'age (OR=4.587, P=0.024), BMI (OR=4.882, P=0.011) as well as 4 or more of the axillary nodes involved (OR=3.143, P=0.013) were independent predicting factors for the success of sentinel lymph node biopsy. Conclusions:Methylene blue dyeing is a reliable method for sentinel lymph node biopsy. The rate of success is associated with patients' age, BMI as well as the number of metastases in axillary lymph nodes.

Objective : To estimate the prevalence and risk factors of suicide ideation,suicide planning and suicide attempts among middle school students and to provide reference for suicide intervention. Methods : The students of grade 7 to 12 in Ningbo were recruited through multi-stage random sampling method. A self-reported questionnaire survey was conducted to collect the information about suicide ideation,suicide planning and suicide attempts within 12 months. A multivariate logistic regression model was used to analyze the risk factors for the three aspects of suicide among middle school students. Results: A total of 10 729 questionnaires were sent out and 10 726 valid ones were collected,with a validity rate of 99.97%. The prevalence rates of suicide ideation,suicide planning and suicide attempts among middle school students during the last 12 months were 12.93%,4.54% and 5.06%,respectively. The results of multivariate logistic regression analysis indicated that females（OR：1.397-1.575,95%CI：1.178-1.927）,students of grade 7 to 9（OR：1.625-1.824,95%CI：1.323-2.082）,poor health condition（OR：1.160-2.131,95%CI：1.005-2.985）,loneliness（OR：1.574-4.423,95%CI：1.221-5.254 ）,desperation（OR：2.796-3.232,95%CI：2.400-3.990）,anxiety（OR：1.890-2.117,95%CI：1.503-2.496）,less than 8 hours a day of sleep（OR：1.152-1.263,95%CI：1.030-1.594）,smoking（OR：1.476-2.074,95%CI：1.055-3.072）,drinking（OR：1.479-1.863,95%CI：1.271-2.296）,fighting（OR：1.716-1.941,95%CI：1.330-2.422）and school bullying（OR：2.254-3.292,95%CI：1.342-5.277）were common risk factors for suicide ideation,suicide planning and suicide attempts;physical activity（OR：0.597-0.720,95%CI：0.474-0.923）was a common protective factor for suicide ideation,suicide planning and suicide attempts. Conclusion Females,lower grade,poor health condition,loneliness,desperation,anxiety,lack of sleep,smoking,drinking,fighting,school bullying and physical activity were influencing factors for suicidal behaviors.