Objective To compare the clinical value of hepatitis C virus (HCV)antibody(HCV-Ab),hepatitis C virus core anti-gen (HCV-cAg),hepatitis C virus ribonucleic acid (HCV-RNA)in diagnosis for hepatitis C.Methods A total of 258 patients with hepatitis C were recruited in this study,HCV-RNA was detected by fluorescence quantitative PCR detection,HCV-Ab and HCV-cAg were detected by the double antigen sandwich ELISA statutory,and the test data was analyze.Results The result of HCV-Ab detection was significant difference with those of HCV-cAg and HCV-RNA detection respectively(P <0.05),HCV-cAg and HCV-RNA detection had no significant difference (P >0.05).Conclusion The coincidence rate of HCV-cAg detection and HCV-RNA detection was high,and complement with HCV-Ab,the early detection could be done to prevent the omission of HCV infection and to improve the detection rate.

Objective To study the release characteristics of colon-targeted pellets of volatile oil of Radix Angelica Sinensis (Danggui)in rats.Methods Rats were administrated intragastrically with the colon-targeted pellets of the volatile oil of Radix Angelica Sinensis.At different time points,the rats were sacri-ficed and their gastrointestinal tracts were resected,in which the contents were collected.Ligustilide,as the index of volatile oli of Danggui to evaluate the release characteristics of the colon-pellets in digestive tracts,was determined using HPLC.Results The linear range of ligustilide in the digestive tract of rats was 1.268 ~507.2 mg/L.Four hours after administration,ligustilide was detected in cecum and colon. And 20 h later,ligustilide could still be detected.That meant the colon-targeted pellets could maintain the drug release for a long time in colorectal sites of rats.Conclusion The prepared Danggui volatile pellets can be used as the carrier for targeting on colon,which can be used in the study of the chemo-pre-vention of inflammation related to colorectal cancer at the late stage.

Objective To optimize the procedure of extracting components from Sophora alopecurcurodies ( Kudouzi) . Methods After external use of the extracts of Kudouzi by four different extraction proce-dures on the footpad inflammation rat model induced by carrageenan, the swelling rate was observed as the therapeutic index. The contents of oxysop hocarpine and oxymatrine of extracts from Kudouzi, as the two chemical indexes, were measured by using HPLC. The extraction procedure was screened by thera-peutic index combined with chemical indexes. Then the parameters of the procedure screened were opti-mized by orthogonal test. Results All the extracts inhibited footpad swelling in rats, and the effect of the ethanol extract was the strongest, and in which the contents of oxysop hocarpine and oxymatrine were the highest. The footpad swelling rate was negatively correlated with the contents of the two chemical in-dexes. The optimized extraction procedure was obtained with 10 times of 80%( V/V) alcohol, 4 hours at 50℃. Conclusion The extraction procedure of Kudouzi was stable and feasible, and the contents of ac-tive components were positively correlated with their effects.

Objective To investigate the effects of azone on transdermal absorption of TCM active ingre-dients with different lipophilicity, and further explore its percutaneous penetration enhancement effects on the complex TCM active ingredients. Methods Five model drugs, namely osthole, tetramethylpyrazine, ferulic acid, puerarin and geniposide, were selected based on their lipophilicity determined by logKo/w, and then tested using in vitro permeation studies in which Franz diffusion cellsand rat skin were em-ployed. Meanwhile, the association between the logKo/w values of model drugs and enhancement ratio ( ER) was established. Results Different concentrations of azone could significantly promote the trans-dermal absorption of all model drugs. The ER value gradually increased with the increase of the azone concentration withina certain concentration range. It, however, started to decrease when exceedinga cer-tain concentration level. The penetration enhancement ratio was roughly in parabolic curve relation with the drug lipophilicity after treatment with azone, and azone could achieve the optimal permeation effect for moderate hydrophilic drugs (an estimated logKo/w value of -0. 5). Conclusion azone could significant-ly promote the transdermal absorption of TCM components with different lipophilicity, and its penetration enhancement ratio was in parabolic curve relation with the drug lipophilicity.