BACKGROUND:Adipose-derived stem cel s have different sources, but it is unclear whether these cel s from different sources have difference in their biological properties.
OBJECTIVE:To detect the in vitro proliferation and chondrogenic differentiation of adipose-derived stem cel s derived from renal fat capsule and groin.
METHODS:Adipose-derived stem cel s from renal fat capsule and groin of rats were isolated, cultured and identified. MTT assay was used to detect in vitro proliferation ability of these cel s. Passage 3 cel s were under chondrogenic induction for 2 weeks. After induction, the expression of type II col agen was observed by immunofluorescence detection, and RT-PCR was employed to detect the expression levels of Aggrecan and type II col agen mRNA in the two groups.
RESULTS AND CONCLUSION:After primary culture and passage, adipose-derived stem cel s from the renal fat capsule and groin of rats exhibited similar morphology, and over 95%of cel s expressed CD44 in the two groups. Adipose-derived stem cel s from two sources showed an S-shaped growth curve in vitro and were positive for type II col agen. After RT-PCR detection, the expression levels of Aggrecan and type II col agen mRNA had no difference in adipose-derived stem cel s from renal fat capsule and groin (P>0.05). Experimental results show that adipose-derived stem cel s from both renal fat capsule and groin exhibit stable growth, rapid proliferation and chondrogenic differentiation under orient induction in vitro, indicating there is no difference between these cel s from two sources.

Carbohydrate residues marked by five biotinylatedlectins (Con A, DBA, PHA, PNA, WGA) and carcinoembryonic antigen (CEA) in intestinal metaplasia and dysplasia adjacent to early gastric carcinoma (EGC) were studied immunohistochemically using ABC technique. The results were as following.The distribution of carbohydrate residues in intestinal metaplasia and dysplasia was different from normal gastric mucosa. The ratio of positivity for DBA and CEA was significantly higher in mucosa adjacent to gastric carcinoma than that in intestinal metaplasia in chronic gastritis and gastric resection margins. The new classification of intestinal metaplasia based on lectin binding demonstrated that the metaplasia was predominantly metaplasia of colonic (ConA + PNA-) and mixed types (ConA +PNA + ) in Carcinoma free gastric mucosa of either intestinal or diffuse carcionma. Some of the diffuse gastric carcinomas may originate from the intestinal meiaplasia.

PURPOSE Expression of Rb and p53 gene in gastric carcinomas and its relationship to prognosis as well as clinico pathology were investigated.METHODS Expression of Rb and p53 products and p53 gene mutation in gastric carcinomas were analysed by means of immunohistochemistry and in situ hybridazation.RESULTS p53 gene mutation was found in 6/20(30%).Over expression of Rb and p53 products was found in 62/85(72.94%) and 42/85(49.41%).Both the positive grades showed significant inverse correlation with patient survival( P

Objective To compare the effect and safety of digital flexible ureteroscopic lithotripsy and per-cutaneous nephrolithotomy for renal calculi. Methods Clinic data of 105 cases with kidney stones were analyzed retrospectively, including 53 cases with digital flexible ureteroscopic lithotripsy (group A) and 52 with minimally invasive percutaneous nephrolithotomy (group B). The operative time, intraoperative blood loss, stone-free rate, complications, average hospitalization time were compared. Results There were no significant differences in age, gender, stone size, stone surface area, average operative time, stone-free rate and complications between two groups. Significant differences were found between group A and group B in terms of intraoperative blood loss and average hospitalization time. Conclusions Digital flexible ureteroscopic lithotripsy has similar effect as minimally invasive percutaneous nephrolithotomy for the treatment of kidney stones about 20 mm in terms of average operative time, stone-free rate, and complications, but excels minimally invasive percutaneous nephrolithotomy in intraopera-tive blood loss and average hospitalization time. Digital flexible ureteroscopic lithotripsy can be the first choice for the treatment of kidney stones about 20 mm.

One hundred and ten cases of early gastric carcinoma (EGC) are analysed pathologically.All cases were operated during the years of 1973-1987 in PLA General Hospital.The rate of EGC in operated gastric cancer cases was about 10% in average.However it was 20.6% in 1987.It occurs frequently among the people over the age of 50 and is usually located at the pyloric antrum.The macroscopica-lly,depressed type (68%) is predominat.The elevated type constitutes only 13.5% of all cases.Histolo-gically,nearly all of the elevated lesions are well differentiated carcinomas,while the majority of the depressed lesions are less or poorly differentiated.It is possible that most of the depressed lesions are the result of the erosion of the neoplastic epithelium by gastric juice.Ulcer cancer is very rare.To examine the specimen carefully and comperhensively is very important in verifying an EGC.The follow-up of the initial 12 cases for a period of about 10 years reveals that there has been no death from gastric cancer.Since patient with EGC who has been operated upon,particularly the elderly,may die of other disease,it is suggested that the prognosis in regard to the gastric carcinoma of the dead should be judged according to the autopsy report.

Objective:To retrospectively evaluate the therapeutic efficacy of two different surgical approaches, Ivor-Lewis esophagectomy and 2-incision esophagectomy via left thoracic-cervical pathway, in the treatment of middle thoracic esophageal squamous cell carcinoma. Methods:One hundred and two patients from 167 patients with middle thoracic esophageal squamous cell carcinoma received Ivor-Lewis esophagectomy and another 65 patients received 2-incision esophagectomy via left thoracic-cervical pathway. The local recurrence rate of tumor and survival rate were calculated by using Kaplan-Meier method. The difference in the survival rate between the two surgical methods was analyzed by using log-rank test. The prognostic risk factors were assessed by COX regression analysis. Results:Peri-operative complications occurred in 35 patients (21.0%), in which the incidences of recurrent laryngeal nerve (RLN) injury and anastomotic leakage were higher in the left thoracic-cervical group (P<0.05), and the incidence of intrathoracic gastric retention was higher in the Ivor-Lewis group, but the difference was not significant. The 3-year local recurrence rate was 37.3% in the Ivor-Lewis group, and 40.0% in the left thoracic-cervical group. The difference was not significant (P>0.05). The overall 5-year survival rate of the 167 patients was 34.6%. It was 36.0% in the Ivor-Lewis group and 32.3% in the left thoracic-cervical group, respectively (P>0.05). COX regression analysis revealed that pTNM staging was the independent prognostic factor [P=0.000, HR(hazard ratio)=2.69]. Conclusion:Both Ivor-Lewis esophagectomy and 2-incision esophagectomy via left thoracic-cervical pathway are feasible alternatives in the treatment of middle thoracic esophageal squmamous cell carcinoma. We should choose the rational operative method based on the patients'individual condition.