Objective To explore the value of sympathetic skin response (SSR) detection for evaluating the autonomic nervous damage in patients with cerebral infarction.Methods 56 cases with cerebral infarction within 2 weeks and 50 cases of healthy controls were detected SSR by EMG,and the results were compared.Results 18 patients with cerebral infarction did not elicit SSR and 18 patients were abnormal with the latency and amplitude of SSR,and the abnormality rate of SSR was accounting for 64.29％,which was significantly different compared with control group( t =10.66,3.83,all P ＜0.01 ).Conclusion The patients with cerebral infarction had severe autonomic dysfunction and SSR,and SSR detection was a simple,safe and noninvasive method,and could quantitatively evaluate the svmpathetic function in patients with cerebral infarction.

Objective To study the pharmacokinetics and bioequiavailability of Glipizide in healthy volunteers. Methods The plasma levels of Glipizide were determined at 30 min,1,2,3,4,5,6,8,10,12,14 h by HPLC after single-dose oral cross administration of 10 mg Glipizide tablets of two brands in 18 healthy male volunteers aged 23 to 25 years old,with the interval of one week. Results Glipizide tablets of the two brands were as follows,Tmax: (2.39?0.50),(2.33?0.49) h; Cmax: (675.3?151.7),(647.1?166.7)g/L; AUC_ 0-t : (3 565.4? 733.4) ,(3 304.8?588.4) ?g?h -1 ?L -1 . Conclusion The two preparations were bioequivalent.

Objective To evaluate treatment effects and to determine the prognostic factors of primary orbital lymphoma. Methods From March 1984 to October 2000, 44 primary orbital lymphoma patients were admitted into our hospital, with 27 males and 17 females. Twenty-seven patients had eyelid or conjunctiva primary, and 17 patients orbit primary. Patient's age ranged from 14 to 86 years (median, 50.5years). According to the Ann Arbor Staging System, there were 40 ⅠEA, 4 ⅡEA lesions. Cobolt-60 ?ray was given first to the whole orbit to 30-35?Gy followed by 180 kv X-rays or 9 Mev ?-beam to the focus to a total dose of 41.0-51.5?Gy (median, 46.5?Gy). Kaplan-Meier method was used to analyze the survival. Log-rank test was used to detect the difference between groups.Results Only 1 patient failed from primary site and 5 patients from distant metastasis after radiotherapy. The 5-year and 10-year cause-specific survival rate were 90.9% and 84.5%, respectively. On univariate and multivariate analyses, eyelid or conjunctiva localization and Ann Arbor stage ⅠEA were independent favorable prognostic factors for survival. The 5-year survival was much higher in eyelid or conjunctiva patients than in orbital ones (96.3% vs 70.6%, P=0.013). The survival time was longer in stage Ⅰ than in stage Ⅱ (P=0.000). Neither the RT dose nor the age or sex influenced the outcome.Conclusions Localized orbital lymphoma can be well controlled with low-dose RT alone. Patients with eyelid or conjunctival localization and stage Ⅰ have better cause-specific survival.

Objective To establish fingerprint of volatile components in golden throat lozenges by GC-MS. Methods Volatile components from 10 batches of golden throat lozenges were extracted by steam distillation method and analyzed by GC-MS with n-tetradecane as internal standard. Fingerprint peak detection and similarity evaluation were applied to the total ion chromatogram(TIC)of GC-MS by "Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine (Version 2004)" ,fingerprint peak was identified by mass spectrometry. Results A GC-MS fingerprint was established based on 10 common peaks as characteristic fingerprint information.The similarity of fingerprint peaks from the 10 batches of samples were more than 0.998.Ten fingerprint peaks were determined by mass spectrometry, all of which were composed of monoterpenes and monoterpenes containing oxygen, the highest content of which was L-menthol containing oxygen monoterpenes, accounting for 83.17% of the total. Conclusion The fingerprint established by gas chromatography-mass spectrometry can completely reflect the volatile components of golden throat lozenges tablets, with strong characteristics and specificity, and can be used as an effective method for the quality control of volatile components inf golden throat lozenges.

Objective To investigate the clinical significance of cortactin antibody in myasthenia gravis (MG).Methods Cortactin antibody in the serum of 100 MG patients, 40 normal controls and 40 other neuroimmune diseases patients was examined by Western blotting and ELISA using purified recombinant human protein cortactin as antigen .Acetylcholine receptor antibody ( AchR-ab ) and muscle specific kinase antibody (MuSK-ab) were parallely measured by ELISA.Results Antibodies to cortactin were found in nine (9％) serum samples of 100 MG patients.Four of the nine cortactin antibody positive sera were also positive for AChR-ab.The rest five MG patients only had antibodies against cortactin ( no detectable AChR-ab or MuSK-ab).None of the control subjects (including 40 normal controls and 40 other neuroimmune diseases patients ) had cortactin antibodies.Most (7/9) of the cortactin antibody positive MG patients presented with early-onset subgroup.Patients only with cortactin antibodies did not appear to have thymoma.Patients with MG who had both AChR and cortactin antibodies showed maximum involvement of muscles and severe Osserman's classification ( three cases of type ⅡB and one case of type Ⅳ) . Conclusion Cortactin antibody may be a new antibody for MG , which can provide clues for further exploring the potential pathogenic mechanisms of the disease .