The article reviewed the common analysis methods, and application status of the surface electromyography (sEMG) in the assessment and treatment of child neurological rehabilitation.

ObjectiveTo investigate the change of Bcl-2 and P53 expresssion after whiter matter damage in neonatal rats. Methods100 rats (2 days old) were randomly divided into two groups: control group and periventricular leucumalacia (PVL) group. Control group received normal saline 5 mg/kg intraperitoneal injection, PVL group received lipopolysaccharide (LPS) 5 mg/kg intraperitoneal injection. The expression of Bcl-2 and P53 was measured 6 h, 12 h, 24 h, 48 h after operation with immunohistochemical staining. ResultsThe expression of Bcl-2 increased 6 h after operation, and to the top 48 h after operation in the PVL group, which was significantly different compared with the control group (P<0.01). The expression of P53 increased at 6 h after operation, and to the top 12 h after operation in the PVL group, which was significantly different compared with the control group (P<0.01). ConclusionThe expression of Bcl-2 and P53 increased in the white matter injuried neonatal rats induced by LPS.

After a description of the demand for medical information in grass-root PLA health units, the experiences of Medical Library of Chinese PLA in providing medical information service for grass-root PLA health units were summarized, and suggestions were proposed for medical library and information institutions to provide information service for them.

Humans ; Hydroxycholecalciferols ; blood ; Pneumoconiosis ; blood

Objective To produce the biological calcium citrate cement with fresh oyster shells, and investigate its compressive strength and biocompatibility so as to provide the experimental basis for clinical application of the material.Methods The compressive strength of biological calcium citrate cement was measured and its surface morphology was observed by SEM.The calcium release curve and pH value were measured in the simulated body fluid.Last, its biocompatibility was detected by cytotoxicity test.Results Biological calcium citrate cement produced by 0.33mL/g liquid solid ratio had the maximum compressive strength, and the crystal structure of the material was uniform and orderly.The determination of pH value showed that the degradation and absorption of biological calcium citrate cement did not significantly change the pH value of the body fluid.With gradual degradation of the material, the concentration of Ca2+ in the solution increased gradually.Cytotoxicity test showed that this material had good biocompatibility and no cytotoxicity.Conclusion Biological calcium citrate cement possesses strong compressive strength and good biocompatibility, and it can form a microenvironment with low in alkaline and high in calcium.

Objective To investigate the bacteriostasis effect of combination of Shuanghuanglian powder with piperacillin/tazobactam or cefoperazone/sulbactam for extensively drug-resistant Acinetobacter bauman-nii in vitro.Methods The minimum inhibitory concentration(MIC)and partial inhibitory concentration(FIC) index of 30 clinical isolates of Acinetobacter baumannii were measured with different concentrations of Shuan-ghuanglian,piperacillin/tazobactam,cefoperazone/sulbactam or combination.The effect of combined medica-tion was determined by FIC index.Results After shuanghuanglian combined with piperacillin/tazobactam,no strain showd synergistic effect;16.7% of the strains showed additive effect;83.3% of the strains showed ir-relevant effect;no strain showed antagonistic effect.After Shuanghuanglian combined with cefoperazone/sul-bactam,23.3% of the strains showed synergistic effect;73.3% of the strains showed additive effect;3.3% of the strains showed irrelevant effect,no antagonistic effect was shown.Conclusion The antibacterial effects of Shuanghuanglian and piperacillin/tazobactam were mostly irrelevant,while the antibacterial effect of Shuang-huanglian and cefoperazone/sulbactam are mostly synergistic and additive effect,w hich had better antibacterial effect to Acinetobacter baumannii in vitro.

Objective To explore antibacterial effect of Shuanghuanglian of Chinese medicine in vitro on acinetobacter baumannii of pan-resistant drugs.Methods Using the broth dilution method,the Shuanghuanglian injection,antimicrobial tests in vitro of Shuanghuanglian in-jection combined with Cefoperazone /sulbactam for the pan-resistant acinetobacter baumannii were conducted .Results Shuanghuanglian MIC for pan - resistant acinetobacter baumannii was 20 mg /mL,effects of Shuanghuanglian combined with Cefoperazone /sulbactam on pan-resistant acinetobacter baumannii greatly reduced compared with single Shuanghuanglian or cefoperazone /sulbactam.Conclusion Shuanghuanglian for pan-resistant acinetobacter baumannii has inhibito-ry effect,the application of traditional Chinese medicine combined with antibiotics can reduce the mount of antibiotics so as to reduce and delay the bacterial resistance.

Objective To explore antibacterial effect of Shuanghuanglian of Chinese medicine in vitro on acinetobacter baumannii of pan-resistant drugs.Methods Using the broth dilution method,the Shuanghuanglian injection,antimicrobial tests in vitro of Shuanghuanglian in-jection combined with Cefoperazone /sulbactam for the pan-resistant acinetobacter baumannii were conducted .Results Shuanghuanglian MIC for pan - resistant acinetobacter baumannii was 20 mg /mL,effects of Shuanghuanglian combined with Cefoperazone /sulbactam on pan-resistant acinetobacter baumannii greatly reduced compared with single Shuanghuanglian or cefoperazone /sulbactam.Conclusion Shuanghuanglian for pan-resistant acinetobacter baumannii has inhibito-ry effect,the application of traditional Chinese medicine combined with antibiotics can reduce the mount of antibiotics so as to reduce and delay the bacterial resistance.

OBJECTIVETo investigate the epidemiological status of cholestasis in first-hospitalized patients with chronic liver disease in Shanghai, and to provide a scientific basis for developing prevention and treatment measures.

METHODSFrom April 2005 to September 2014, 5,146 first-hospitalized patients in Shanghai with a diagnosis of chronic liver disease were enrolled in this study. Clinical data of the 4,660 patients who fit the study criteria for participation were collected for retrospective analysis.Diagnosis of cholestasis was made according to serum alkaline phosphatase (ALP) levels higher than 1.5 times the upper limit normal (ULN) and gamma-glutamyltransferase (GGT) levels higher than 3 times the ULN. The incidence rate of cholestasis was assessed for relation to age, sex, etiology, and type of liver disease, and statistically compared to the general clinical data and specific biochemical indicators with potential sex-related differences. T-test and chi-square test were performed for the statistical analyses.

RESULTSOf the 4,660 study participants, 10.26% had cholestasis; the prevalence of cholestasis increased with increasing age in male patients. The distribution of the cholestasis incidence according to the type of chronic liver disease was: 75.00%, primary sclerosing cholangitis; 42.86%, primary biliary cirrhosis; 35.97%, hepatic tumor; 30.77%, autoimmune hepatitis; 28.31%, drug-induced liver disease; 16.46%, alcoholic hepatitis; 13.98%, cryptogenic cirrhosis; 12.99%, schistosomal cirrhosis; 7.53%, alcoholic cirrhosis; 7.32%, mixed cirrhosis; 5.94%, viral liver cirrhosis; 2.70%, nonalcoholic fatty liver disease. There was no significant difference in the prevalence of cholestasis between the two sexes. In the patients with cholestasis, the levels of GGT and total bilirubin were significantly different between the two sexes.

CONCLUSIONThe incidence rate of cholestasis in first-hospitalized patients with chronic liver disease was 10.26%, and the rate increased with increased age. Patients with primary sclerosing cholangitis or primary biliary cirrhosis had higher incidence rates of cholestasis. Incidence rates of cholestasis of the various chronic liver diseases were not related to sex.

Bilirubin ; China ; Cholestasis ; Chronic Disease ; Humans ; Incidence ; Liver Diseases ; Male ; Prevalence ; Retrospective Studies ; gamma-Glutamyltransferase

Objective To review the clinical-pathological features, the tendency of incidence over 20 years, the predisposing factors, and the differences between the cases of cutaneous malignant melanoma from two hospitals. Methods A collection of 305 cases diagnosed as cutaneous malignant melanoma, among which 185 cases had complete clinical-pathological data, during 1981-2000 was analyzed. Results Acral malignant melanoma accounted for 63.3%, and the cases associated with congenital small nevi at the primary site accounted for 15.8% of 305 patients. During the period 1981-1990 and 1991-2000, cutaneous malignant melanoma constituted 0.053% and 0.094%, respectively, of all diagnoses with pathological sections, with an growth rate of 3.9% yearly. There was a tendency of the increased lesions located on face and neck, and decreased lesions on acra, over 20 years. Conclusion A rise of diagnosis of cutaneous malignant melanoma has been noticed from two hospitals over 20 years. Acra, especially planta, is the predominant anatomical site of cutaneous malignant melanoma.