Adult ; Coal Mining ; Disability Evaluation ; Humans ; Male ; Middle Aged ; Pneumoconiosis ; physiopathology ; Respiratory Function Tests

Based on the counting results from nutrition agar in our study, and compared with another two kinds of coliform bacteria detective plates for dinner and drinking sets which were provided by two manufactures and inspected by China National Health Bureau , we accomplished the detectability test of the Huankai coliform bacteria plates. In our study, 3kinds of cell density were adopted: 10cells/mL, 50cells/mL and 100cells/mL . The results shew that the detectability of Huankai plates basically conformed with that of nutrition agar , and was no evident difference from that of another two kinds of plates. The conclusions were that Huankai plates can completely be used in the inspection and test for conform bacteria.

0.05)].Maternal serum concentrations of TG and LDL-C were significantly increased in DM group [(3.6?0.9)and(4.8?0.6)mmol/L] compared with GIGT group [(2.7?0.7)and(3.8?0.9)mmol/L] and GDM group [(2.9?0.7)and(3.7?0.8) mmol/L](P0.05).The incidence of fetal distress in the GIGT group(9.8%)was lower than that in DM group(20.2%)and GDM group(21.4%,P

AIMOur previous studies showed that electroacupuncture (EA) could inhibited radiant heat induced pain and acute or chronic inflammatory pain in rats. In the present study, we observed whether EA with different frequencies could suppress neuropathic pain.

METHODSL5/L6 nerve ligation model was used to assess the effect of EA on neuropathic pain. Mechanical allodynia was represented by 50% withdrawal threshold, while cold-induced ongoing pain was detected by the number of paw lift in 5 min when the rat was put on a 5 degrees cold plate. Han's acupoint nerve stimulator (HANS) was connected to needles inserted into acupoints "jiaji" and "Zusanli" in both sides. The parameters were: (intensity: 0.5-1-2 mA, 10 min each; frequency 2 Hz or 100 Hz; pulse width: 0.6 ms for 2 Hz, 0.2 ms for 100 Hz).

RESULTSEA of both 2 Hz and 100 Hz could relieve the mechanical allodynia, where 2 Hz could induce the effect with shorter latency; they could also relieve the cold-induced ongoing pain, where the effect of 2 Hz outlasted the EA session by up to 48 h after repetitive stimulations over several weeks; a significant relieving effect on cold-induced ongoing pain could also be induced by needle insertion without stimulation.

CONCLUSIONEA could relieve neuropathic pain, the analgesic effect of 2 Hz EA is higher than 100 Hz EA.

Animals ; Electroacupuncture ; Male ; Neuralgia ; therapy ; Rats ; Rats, Sprague-Dawley

Objective: To explore the points selection pattern of acupuncture for sleep apnea syndromes by data mining technique. Methods: Clinical literature about acupuncture therapy for sleep apnea syndromes was derived from China National Knowledge Infrastructure (CNKI), Wanfang Academic Journal Full-text Database (Wanfang), Chongqing VIP Database (CQVIP), PubMed and Science Direct between the time that databases were created and March 25th,2017. Relevant excel database was established and descriptive studies and association rules were analyzed. Results: The most frequently used point was Lianquan (CV 23) and the most frequently used meridian was the Stomach Meridian. The analysis of association rules showed that the clinical choice of acupuncture points was highly correlated, among which the combination of the highest degree of confidence and the highest degree of support was Shenmen (HT 7) and Sishencong (EX-HN 1); Lieque (LU 7), lianquan (CV 23) and Zhaohai (KI 6). Conclusion: Acupuncture treatment of sleep apnea syndromes has specific selection rules of points, providing certain references for clinical and scientific research.

ObjectiveTo establish a new method for preparing synaptosomes.MethodsDensity gradient centrifugation method was used to isolate synaptosomes of mouse, checking by transmission electron microscopy.ResultsSynaptosomes prepared by this method had intact morphological characteristics, surrounding with a continuous oval-shaped membrane structure, moreover, mitochondrion and lots of synaptic vesicle in them.ConclusionThis method is applicable to establish a rapid, convenient and useful method for preparing synaptosomes.

A bacterial strain DN25, effective on cyanide-degradation, was isolated from contaminated soil and identified as Alcaligenes sp. on the basis of phenotype analysis and 16S rDNA sequence analysis. It showed great tolerance to the cyanide, which can grow in the medium containing 500mg CN -/L. The suitable condition for the cell growth and boitransformation was pH8.0 and 30oC and the transformation rate for 500mg CN - /L could achieve 99% in 10 h. It has also been found that the screened strain had the ability of K 4Fe(CN) 6 transformation with 96% of transformation rate at 12 h for the concentration of 500 mg CN /L.

0.05);however there were significant difference between D4T+DDI+NVP group and AZT+DDI+NVP group(P

Ten flavonoids were isolated from the 95% ethanol extract of the seeds of Alpinia galanga Willd. with a combination of various chromatographic techniques, including silica gel, Sephadex LH-20 and preparative HPLC. On the basis of spectroscopic data analysis, they were elucidated as (2R, 3S)-pinobaksin-3-cinnamate (1), (2R, 3R)-pinobaksin-3-cinnamate (2), pinocembrin (3), pinobaksin (4), 3-O-acetylpinobaksin (5), galangin (6), galangin-3-methylether (7), kumatakenin (8), 3-methylkaempferol (9) and (2R, 3R)-3, 5-dihydroxy-7-methoxyflavanone (10). Among them, compound 1 is a new compound, compounds 2, 5 and 10 were isolated from the genus Alpinia for the first time, and others were isolated from this plant for the first time.
Alpinia ; chemistry ; Benzopyrans ; chemistry ; isolation & purification ; Cinnamates ; chemistry ; isolation & purification ; Flavanones ; chemistry ; isolation & purification ; Flavonoids ; chemistry ; isolation & purification ; Kaempferols ; chemistry ; isolation & purification ; Molecular Structure ; Plants, Medicinal ; chemistry ; Seeds ; chemistry

OBJECTIVETo study the clinical feature, coronary artery lesion specialty and Chinese medicine syndrome characteristics of coronary heart disease (CHD) in females.

METHODSThe clinical materials of 297 female patients with CHD, whose diagnosis had been confirmed by coronary angiography, were analyzed and compared with those of 772 male patients.

RESULTSCHD in females was characterized by later occurrence, frequently complicated with diabetes mellitus and blood lipid disorder, and rather serious coronary lesion, mainly the multi- or double-branch lesion, involving the anterior descend branch, right coronary artery and convolution branch; with the often encountered syndrome factors and their combinations of qi deficiency, blood stasis, yin deficiency, blood stasis due to qi deficiency,deficiency of both qi and yin,turbid phlegm, yang-deficiency, etc.; the figures of tongue and pulse were generally dark-red or dark-purple or pale-red colored corpulent tongue proper, with waterless yellow or less coating, even without coating, cyanosed varicose sublingual veins,thin-weak or thin- rapid pulse.

CONCLUSIONFemale CHD has its special clinical feature, coronary specialty and Chinese medicine syndrome characteristics. Proper treatment methods for it are supplementing qi, activating blood, nourishing yin in dominance, with dissolving phlegm and accessing yang as accessory.

Adult ; Age of Onset ; Aged ; Coronary Artery Disease ; diagnosis ; epidemiology ; Coronary Disease ; diagnosis ; epidemiology ; Diagnosis, Differential ; Female ; Humans ; Male ; Medicine, Chinese Traditional ; methods ; Middle Aged ; Sex Factors ; Yin-Yang