Objective To evaluate the value of serum tumor markers in diagnosis of over 80 years old patients with colorectal cancer.Methods Serum CA199,CA242 and CEA of 68 cases senile patients with colorectal cancer and 35 cases senile patients with benign large intestinal diseases were detected by ELISA.Results Serum levels of CA199,CA242 and CEA in patients with colorectal cancer were (37.95 ± 15.95 ) U/ml,( 50.08 ± 24.20 ) U/ml,( 22.34 ± 17.35 ) μg/L respectively and were significantly higher than that in patients with benign large intestinal diseases CA199 ( 10.73 ± 6.27 ) U/ml,CA242 ( 8.00 ± 3.52 ) U/ml,CEA(2.32 ± 1.01 )μg/L.In addition,the level of these three markers was increased with the progress of colorectal cancer and the sensitivity of diagnosis was related with the stages of colorectal cancer.Parallel combined testing methods increased the diagnostic sensitivity to 83.6％,and serial combined testing increased the diagnostic specificity to 88.7 ％.Conclusions Serum tumor markers showed certain values in the diagnosis of the senile patients with colorectal carcinoma.Furthermore,the combined determination of tumor markers is helpful for early diagnosis of colorectal cancer and increases the sensitivity and specificity in diagnosing of colorectal carcinoma.

In order to investigate the role of endothelin (ET) in blood pressure re-gulation, the depressor effect of ET_A receptor antagonist -BQ_(123) was observed. After in-travenous administration of BQ_(123) (0.1, 1.0 and 2.0 mg/kg) into spontaneously hyperten-sive rat(SHR) and normotensive WKY rat, their blood pressure decreased in a dose-depen-dent manner, which continued 40-60 min. The blood pressure reducing action of BQ_(123)in SHR was more potent than that in WKY rat. Intravenous injection of BQ_(123) intoWistar rat inhibited pressor reponse induced by exogenous ET-1(2?10~(-9)mol/kg) in adose-dependence manner. ET-1(10~(-7)mol/L) stimulated proliferation of cultured smoothmuscle cells from SHR aorta, i.e., increase in ~3H-thymidine and ~3H-Leucine, however, BQ_(123)effectively antagonized the above action of ET-1. These results suggested that endogenousET to some extent could be involved in the regulation of normotension and also in thedevelopment of hypertension, and that ET antagonists would be useful for clinical preven-tion and therapy of hypertensive diseases.

Objective:To investigate the correlation between anterior cruciate ligament injury and the bone contusion in the posterior of tibial plateau and the risk factors of it.Methods:Data of patients with knee trauma who came to our hospital from Oct. 2017 to Nov. 2019 were retrospectively analyzed. There are 100 males and 55 females with an average age of 40.0±10.6 years (range, 18-60). Bone contusion in the posterior tibial plateauand anterior cruciate ligament, posterior cruciate ligament, medial collateral ligament, lateral collateral ligament and fibula injury were evaluated through imaging data, and injury mechanism, age, sex and the side of injury were collected from medical records. The effect of events on bone contusion in the posterior of tibial plateau was analyzed.Results:Bone contusion occurred in 92 of the 155 patients (56 males and 36 females), with an average age of 39.6±10.5 years (range, 19-55 years). There were 45 cases on right knee and 47 cases on the left side. ACL injury: ACL was completely ruptured in 42 cases, partially ruptured in 34 cases, and had no injury in 16 cases. There were 18 cases with PCL injury, and 74 cases without any injury. There were 57 cases of non-contact injury, and 35 cases of contact injury. There were 57 cases with fibular head injury, and 35 cases without. There were 44 cases with MCL injury, and 48 cases without. There were 13 cases with LCL injury, and 79 cases without. ACL injury degree ( χ2=18.345, P<0.05) and the head of fibula ( χ2=5.495, P<0.05) injury were significantly different between the two groups. There was no significant difference in male ratio ( χ2=1.315, P>0.05), knee side ( χ2=0.880, P>0.05), injury mechanism ( χ2=3.467, P>0.05), age ( t=0.664, P>0.05), LCL ( χ2=0.010, P>0.05)、MCL ( χ2=0.029, P>0.05) and PCL ( χ2=0.161, P>0.05). ACL injury was an independent risk factor for bone contusion in the posterior of tibial plateau ( P<0.05) and the injury of fibula head was an independent risk factor for the formation of bone contusion in the posterior of tibial plateau ( P<0.05) according to logistic analysis. The possibility of bone contusion on the posterior tibial plateau in ACL complete injury was significantly greater than that of ACL partial injury ( OR=2.695, P=0.021). Conclusion:The injury of ACL and fibula head are independent risk factors for the bone contusion in posterior of tibial plateau; ACL complete injury is more likely to be associated with posterior tibial plateau bone contusion than ACL partial injury.

Objective To summarize the experiences in diagnosis and treatment of thymoma in 120 cases. Methods 120 patients with thymoma were operated on, and the diagnosis was confirmed pathologically, from January 1994 to December 2003 in our department. According to Masaoka clinical and pathological classification, 54 cases were stage Ⅰ, 32 were stage Ⅱ, 22 were stage Ⅲ, 12 were stage Ⅳ. In 42 cases there were symptoms of myasthenia gravis. Result Total excision was performed in 112 cases and partial excision in 8 cases. When the tumor was beyond stage Ⅱ, postoperative radiotherapy was given. Recurrence occurred in 5 patients 1-7 years after operation. Conclusions Surgical resection is currently the main therapeutic method for tumor of the thymus. The preferred surgical approach was excision of thymoma and removal of perithymic fat in the anterior mediastinum through a median sternotomy. Thymoma is a potentially malignant disease, and the patients with the tumor beyond stage Ⅰ must undergo postoperative radiotherapy. Local recurrence of the tumor should be surgically excised.

Objective To observe clinical effects and side reactions of Huayu pill combined with TC (Paclitaxel+Carboplatin) chemotherapeutic regimes on treating epithelial ovarian cancer. Methods All 60 patients with the epithelial ovarian cancer were randomly divided into the treated group (Huayu pill combined with TC chemotherapeutic, n=30) and the control group (TC chemotherapeutic regimes, n=30). Results The effective rate of the treated group and the control group was 46.7% and 37.9% respectively. The objective response rate (CR+PR+SD) was 86.7% in the treated group, while this rate was 68.9% in the control group (P

Purpose To observe the effect of paeonol on inflammatory factors after focal cerebral ischemiareperfusion injury in rats and its possible mechanism of paeonol.Methods Focal cerebral ischemic model was established by MCAO in rats.After 2 hours of cerebral ischemia and 22 hours of reperfusion,the expression of TNF-α and IL-1β in brain was determined by immunohistochemistry.The content of TNF-α and IL-1β in serum was measured by ELISA.Results Compared with model group,the levels of TNF-α and IL-1β in brain and serum in rats in the paeonol groups were obviously decreased.Conclusion Paeonol could relieve brain damage induced by focal cerebral ischemia-reperfusion,which may be related to inhibiting the production of inflammatory factors.

The high frequency subject headings were extracted from papers published in Journal of American Medi-cal Informatics Association from 2006 to 2015 to form a matrix of high frequency subject headings and their source papers. The co-occurrence of high frequency subject headings in a paper was clustered and analyzed, which showed that studies in Journal of American Medical Informatics Association from 2006 to 2015 were focused on the design and development of clinical software or platform, application of data mining in medical field, and clinical decision-making support system.

OBJECTIVE：To observe effect of L-NAME on apoptosis in the gerbil hippocampus after recirculation following ischemia.METHOD：Bilateral occlusion of common carotid arteries and TUNEL stain methods in gerbitil hippocampal tissue slice were used.RESULTS：L-NAME inhibited apoptosis in the CA1 subfield.CONCLUSION:Endogenous NO mediated apoptosis is inhibited by the L-NAME.

Objective To evaluate the effects of ulinastatin on proinflamatory cytokines and oxygen free radical during orthotopic liver transplantation (OLT). Methods Eighteen ASA Ⅲ-Ⅳ patients with end-stage liverⅣ diseases, undergoing OLT were randomly divided into two groups: (1) ulinastatin group received intravenous infusion of ulinastatin 2? 105 IU in 100 normal saline after skin incision and every 4 hours thereafter (group U n = 9); control group received same amount of normal saline instead of ulinastatin (group C n = 9). Anesthesia was induced with midazolam 0.1-0.2 mg?kg-1 , fentanyl 5 ?g?kg-1 and pipecuronium 0.1 mg?kg-1 and maintained with isoflurane inhalation and intermittent iv boluses of fentanyl and pipecuronium combined with epidural anesthesia(T8.9). The patients were mechanically ventilated with 100% O2 and PETCO2 was maintained at 35-40 mm Hg during operation. Swan-ganz catheter was inserted via right internal jugular or subclavian vein after induction of anesthesia. Cardiac output, mixed venous oxygen saturation and central venous temperature were continuously monitored with continuous cardiac output monitor (Baxter, Vigilance). ECG,CVP,SpO2 and PETCO2 were also continuously monitored during operation. Radial artery was cannulated for continuous direct blood pressure monitoring. Blood samples were taken before skin incision (T0), 120 min after skin incision (T1), 30 min after liver was removed ( anhepatic phase) (T2) , 5 min and 60 min after reperfusion of the graft (T3 , T4) and at the end of operation (T5 ) for determination of plasma IL-6, IL-8, TNF-? and MDA concentration. Body temperature was maintained above 35.5℃ during operation. Venovenous bypass was performed during anhepatic phase. Results (1) In group C plasma IL-6 and Ⅱ-8 concentrations were significantly increased from T1-5 during operation as compared with the baseline values (T0), whereas plasma levels of TNF-? and MDA did not change significantly before and during anhepatic phase (T1 , 2) but were significantly increased during neohepatic phase and at the end of surgery (T3 ,45) as compared with the baseline values (T0).(2) In group U plasma IL-6, IL-8, TNF-? and MDA concentrations were not significantly increased during operation, except that plasma IL-6 and IL-8 concentrations were significantly higher at T3 (5 min after reperfusion of the graft) than the baseline values. Conclusion Ulinastatin inhibits release of proinflammatory cytokines and reduces production of oxygen free radicals during OLT.

Objective To provide decision-making information for the spread of techniques that improve the quality of neonates. Methods A systematic study was made of the political, technical, economic and social viability of neonatal disease screening in China by means of economic and policy evaluation, sociological analysis, and statistical analysis. Results Neonatal disease screening was conducted in 29 provinces throughout the country. However, there was a great difference between the provinces in screening coverage. In order to strengthen control, 16 provinces issued methods or regulations concerning neonatal disease screening. Since the Ministry of Health did not issue relevant control methods that would standardize the responsibilities of the organizations undertaking the screening, problems affecting the safety and effectiveness of neonatal disease screening became increasingly prominent. Conclusion It is necessary and viable to conduct neonatal disease screening in China. But macro control of the work by the government needs to be reinforced. The Ministry of Health ought to issue as soon as possible methods for controlling neonatal disease screening and relevant technical criteria. At the same time publicity of the work needs to be strengthened in society so as to give it impetus throughout the country.