1.Screening and Expression of Recombinant Human Monoclonal Antibody Fab Fragments Specific to Entamoeba histolytica
Qing SHU ; Hongxia SHAO ; Tachibana HIROSHI ; Xunjia CHENG
Chinese Journal of Parasitology and Parasitic Diseases 1987;0(02):-
Objective To prepare recombinant human monoclonal antibody Fab fragments specific to the surface antigen of Entamoeba histolytica. Methods Total RNA was isolated from lymphocytes which were separated from an asymptomatic E. histolytica cyst carrier. The genes of IgG light chain and Fd region of heavy chain were amplified by a reverse transcriptase PCR and ligated with a plasmid vector. After the genes were introduced into Escherichia coli, the clones expressing Fab fragments specific to the surface antigen of E. histolytica were screened and the product was purified. Results Thirty thousand clones were screened and one of them was proved positive to the surface antigen of E. histolytica. Conclusion This study demonstrated that the bacterial system can be used to produce recombinant human monoclonal antibody Fab fragments specific to the surface antigen of E. histolytica and they may be applicable for the future diagnosis and treatment of the infection.
3.Advances in research of reverse genetics of influenza virus.
Cong-Sheng CHENG ; Yue-Long SHU ; Zhi-Qing ZHANG
Chinese Journal of Virology 2007;23(1):68-71
Animals
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DNA, Complementary
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genetics
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Genome, Viral
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Humans
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Influenza Vaccines
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genetics
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immunology
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Orthomyxoviridae
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genetics
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immunology
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RNA, Viral
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genetics
4.Appearances of lung sarcomatoid carcinoma on chest X-ray and CT
Hui-Zhang LI ; Wei LI ; Chong-Qing YANG ; Ji-Shu PAN ; Qi-Hang CHEN ; Cheng ZHOU ;
Chinese Journal of Radiology 2001;0(07):-
Objective To investigate the imaging features of sarcomatoid carcinoma(SC)of lung on chest radiography and CT and to improve knowledge of SC.Methods The chest plain films,CT images and clinical data of all 7 cases of pathologically proved lung SC were retrospectively analyzed.Results All cases presented with a spheroid solid lung mass ranged from 3 to 11 cm in diameter(average 7 cm).All the lesions were located in middle and lower fields of the lung:3 lesions in right lower lobe,2 in right middle lobe,and the other 2 in left lower lobe.Among the 7 cases:6 cases were of peripheral type and 1 case was of central type.The peripheral-typed lesions of 5 cases had clear margin and 3 of them were lobulated.Three massess were homogenous in density,and the other 4 were inhomogeneous in density with formation of central cavity or calcification.After intravenous contrast administration.Three lesions showed homogeneous enhancement,and the other 4 lesions had inhomogeneous enhancement.The lesions with diameter larger than 6 cm showed marginal or patch-like enhancement.Five cases had pleural or chest walls invasion.Two cases had mediastinal lymph nodes metastases.One case had multiple remote metastasis.Conclusion There are some relatively specific features of lung SC on chest radiography and CT,which may be helpful for diagnosis.
5.Association between genetic variation in PAF-AH V279F and coronary artery disease
Hui-Ping ZHANG ; Fu-Cheng SUN ; Shu WANG ; Qing HE ; Fu-Sui JI ; Feng XU
Chinese Journal of Geriatrics 2003;0(12):-
Objective To explore the relationship between genetic variation in PAF-AH V279F and coronary heart disease among Han population in Beijing.Methods A case-control study was held which enrolled 124 patients with coronary heart disease and 103 normal subjects.The genotype of PAF-AH V279F was determined with allele-specific polymerase chain reaction(AS-PCR)method. Results The highest frequency of PAF-AH V279F genetic variation was VV genotype(92.2%),the next was VF genotype(5.8%)and the lowest was FF genotype(2.0%)among the studied Han population in Beijing.In the coronary heart disease group the frequency of 279 V→F carriers was significantly higher than in the control group(19.3% vs.7.8%,P<0.05)and F allele frequency was also higher(12.1% vs.4.9%,P<0.01).Among the coronary heart disease group,the V279F variation frequency and the F allele frequency were significantly higher in patients with myocardial infarction than in those without myocardial infarction(27.3% vs.13.0%,17.3% vs.8.0%,both P<0.05).In multiple logistic regression analysis,the odds ratio(OR)of V279F genetic variation for coronary heart disease was 1.919(95% CI:1.448-2.544,P=0.033).Conclusions The PAF-AH V279F genetic variation may be a novel genetic marker for high risk of coronary heart disease.
6.Quality control of Maca (Lepidium meyenii).
Ji-cheng SHU ; Hang-qing CUI ; Ying-zheng HUANG ; Xiao-ying HUANG ; Ming YANG
China Journal of Chinese Materia Medica 2015;40(23):4536-4540
To control the quality of Maca, the quality standard was established in this study. According to the methods recorded in the Appendix of Chinese Pharmacopoeia (2010 Edition), the water, extract, total ash, acid insoluble substance, and heavy metals inspections in Lepidium meyenii were carried out. N-benzyl-9Z, 12Z-octadecadienamide in L. meyenii was identified by TLC, and it was determined by HPLC. The results showed that the N-benzyl-9Z, 12Z-octadecadienamide identification of TLC was a strong mark and specificity. In content determination experiment, the linearity of N-benzyl-9Z, 12Z-octadecadienamide was in the range of 0.01-2 microg (r = 0.9998), and the average recovery (n=9) was 99.27% (RSD 2.0%). The methods were simple, accurate, with good reproducibility. It is suitable for quality control L. meyenii.
Chromatography, High Pressure Liquid
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methods
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Chromatography, Thin Layer
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methods
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Lepidium
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chemistry
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Plant Extracts
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chemistry
8.Prepatellar synovial hemangioma: a case report.
Zhi-Shan CHENG ; Shu-Min JIA ; Jun-Qing XIN ; Bo JING ; Jie LIU ; He-Yu ZHOU
China Journal of Orthopaedics and Traumatology 2013;26(3):221-222
Hemangioma
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pathology
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Humans
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Joint Diseases
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pathology
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Male
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Middle Aged
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Synovial Membrane
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pathology
9.Significance of C-reaction protein for differential diagnosis of fever after chemotherapy on children with acute lymphoblastic leukemia.
Cheng-qing FANG ; Yong-min TANG ; Hai-feng LI ; Hua SONG ; Shu-wen SHI ; Shi-long YANG ; Wei-quan XU
Chinese Journal of Pediatrics 2004;42(7):536-537
Antimetabolites, Antineoplastic
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adverse effects
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therapeutic use
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C-Reactive Protein
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analysis
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Child
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Child, Preschool
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Cytarabine
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adverse effects
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therapeutic use
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Diagnosis, Differential
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Female
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Fever
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blood
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chemically induced
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diagnosis
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Humans
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Infant
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Male
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Precursor Cell Lymphoblastic Leukemia-Lymphoma
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blood
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drug therapy
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Teniposide
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therapeutic use
10.Expression of MRP1/CD_9 in cervical squamous cell carcinoma tissue and its clinical significance
Xiao-Ming SHU ; Run-Yuan JI ; Cheng-Hai WANG ; Xiao-Ning LI ; Jian WU ; Zhen-Qing FENG ;
Cancer Research and Clinic 2006;0(12):-
Objective To investigate the clinical significance in MRP1/CD_9 expression in cervical squamous cancer tissues and normal cervical tissues.Methods The expression of MRP1/CD_9 were assayed by SABC immunohistochemical methods in 53 cases of cervical cancer tissues and 13 cases of normal cervical tissues.Results Positive expression of MRP1/CD_9 was detected in 13 normal cervical tissue.MRP1/D_9 ex- pression is down-regulated in cervical carcinoma(P