Objective： The current study was designed to investigate SSTR subtype gene expression in tissue of primary hepatic carcinoma. Methods： The expression of SSTR-2 and SSTR-3 mRNA in the tumor tissue of hepatic carcinoma and the adjacent tissue of cancer in 27 patients with primary hepatic carcinoma was examined by RT-PCR. Results： The positive rate of expression of SSTR-2 mRNA in the tissues of hepatic carcinoma was 81.5％ ( 22/27) , and the positive rate of expression in the adjacent tissues of cancers was 96.3％ (26/27). The positive rate of SSTR-3 mRNA in tumor tissues and the adjacent tissues of hepatic carcinoma were 66.7％ ( 18/27) and 51.9％ (14/27), respectively. Conclusion： There are more than one SSTR subtype genes expression in tumor tissues of primary hepatic carcinoma.

Objectives：This study was designed to investigate the regulation effect of 8-Br-cAMP （ 8-Bromo 3′ ：5′ -cyclic adenosine monophosphate） on the expression of connexin gene (Cx43) in human tongue carcinoma cell line (Tca8113) . Methods：The inhibitory effect of 8-Br-cAMP on growth of Tca8113 cell line was examined by methyl thiazolyl tetrazolium (MTT) assay. Cx43 gene expression in Tca8113 cells after treated with 8-Br-cAMP was analyzed by immunocytochemistry and flow cytometry . Results：After treated with 8-Br-cAMP(10-5 mol/L, 10-4 mol/L, 10-3 mol/L), Tca8113 cells was inhibited and the inhibition rate was increased with the concentration of 8-Br-cAMP; Immunocytochemistry showed Cx43 protein detectability and up-regulation in 8-Br-cAMP treated cells; The positive rate of Cx43 protein in Tca8113 ce1ls increased from 4.8％ to 26.5％ . There was significant difference between 8-Br-cAMP treated cells and untreated cells （ P<0.01） . Conclusion： The anti-tumor effect of 8-Br-cAMP on Tca8113 cells might be due to up-regu1ation on Cx43 gene.

ObjectiveTo analyze the risk factors for in-hospital mortality in patients with Takotsubo Syndrome （TTS）. MethodsHospitalization data of consecutive patients with TTS from February 2009 to January 2022 were retrospectively collected and analyzed. Patients were divided into survival group and death group according to outcomes. The basic clinical information， triggering factors， laboratory examinations， electrocardiogram， echocardiography， complications and treatments of the two groups were compared. Univariable logistic regression analysis was used to screen the possible risk factors for in-hospital mortality in TTS patients， and multivariable logistic regression analysis was used to determine the independent risk factors for in-hospital mortality in TTS patients. ResultsA total of 62 TTS patients were included in our study， including 21 males （33.9%）， 41 females （66.1%） and 26 postmenopausal women （41.9%）， with the mean age of （55.6±16.2） years， and physical triggers were found in 50 patients （80.6%）. 17 patients （27.4%） died while 45 patients （72.6%） survived during hospitalization. The death group had lower systolic blood pressure and left ventricular ejection fraction （LVEF）， higher incidence rate of syncope， higher level of N-terminal pro-B natriuretic peptide （NT-proBNP） and hypersensitive troponin T （hs TnT） when compared with survival group （all P value <0.05）. As for the triggering factors， the proportion of TTS induced by neurologic disorders in the death group was higher than that in the survival group （P<0.05）. The death group had higher rates of cardiogenic shock， malignant ventricular arrhythmia， atrial fibrillation， and respiratory failure （all P value <0.05）. Compared with the survival group， therapeutic dopamine， therapeutic norepinephrine， hemodialysis and mechanical ventilation were higher in the death group （all P value <0.05）. Univariable logistic regression analysis suggested that syncope， NT-proBNP， LVEF， neurologic disorders， cardiogenic shock， malignant ventricular arrhythmia， atrial fibrillation， respiratory failure， therapeutic dopamine， therapeutic norepinephrine， hemodialysis and mechanical ventilation were potential risk factors for in-hospital mortality in TTS patients （all P value <0.05）. Multivariable logistic regression analysis indicated that neurologic disorders ［OR（95%CI）=5.651（1.195，26.715），P=0.029］， atrial fibrillation ［OR（95%CI）=6.217（1.276，30.298）， P=0.024）］ and therapeutic norepinephrin ［OR（95%CI）=8.847（1.912，40.949）， P=0.005］ were independent risk factors for in-hospital mortality in TTS patients. ConclusionsNeurologic disorders， atrial fibrillation and therapeutic norepinephrin are independent risk factors for in-hospital mortality in patients with Takotsubo Syndrome. Clinically， attention should be paid to the prevention and treatment of neurologic disorders and atrial fibrillation； norepinephrine should be carefully used in patients with diagnosed TTS complicated with hemodynamic instability.

【Objective】To evaluate the role of serum antimullerian hormone （AMH） concentration in predicting clinical outcomes in controlled ovarian stimulation and intrauterine insemination （IUI）. 【Methods】 A retrospective analysis of IUI data from patients with polycystic ovary syndrome and infertility in our reproductive center from January 2014 to August 2017. According to the clinical outcomes，data was divided into three groups：clinical pregnancy group，non-pregnant group，and cancellation cycle group due to multiple follicular or no dominant follicular develop. The ovarian function evaluation indexes were compared such as serum AMH，basal FSH and basal sinus follicle（AFC）. The cutoff values of the AMH to predict multi- follicular development or no dominant follicular development which IUI cycles were cancelled，and to predict obtain cumulative clinical pregnancy outcomes were calculated according to the ROC curve to cancel the cycle. 【Results】 The clinical pregnancy rates of PCOS patients were from 15.9% to 17.1% ，while the cumulative clinical pregnancy rate increased in repeat cycles （≤3 cycles） was 21.6% ，significantly higher than the average clinical pregnancy rate（16.7%）during the first cycle. AMH levels in the cancelled cycle group were significantly higher than those in the non-cancelled cycle group［（14.1±6.5）vs（10.3±4.3）ng/mL，（14.1±6.5）vs（9.3±4.3）ng/mL， P<0.025］. Compared with the clinical pregnancy group，cancel cycle group and non- pregnant group，the mean AFC of the former two groups were significantly higher［（34.5±11.4）vs（30.7±11.3），（7.8±10.8）vs（30.7±11.3），P<0.025］. In addition，we found that women′ s age，baseline FSH were negatively correlated with the cumulative pregnancy rate of repeated IUI cycles，and women′s baseline BMI，baseline LH，AFC，and AMH were positively correlated with cumulative pregnancy rates. The number of mature follicles on the HCG triggering day，as well as the value of AMH，may be the influencing factor of IUI cumulative clinical pregnancy. By ROC curve analysis，we assume that AMH is more suitable than the AFC to predict IUI cycle cancellation rate and the cumulative pregnancy rate.【Conclusion】Repeated IUI cycles can be improved cumulative pregnancy rate of PCOS infertile couples. AMH，as an important index to measure ovarian reserve function，can be used to predict the the IUI cumulative clinical pregnancy outcomes and cancelled cycle rates：as AMH increased to more than 6.56 ng/mL，the pregnancy rate increased；but when AMH ≥14.72 ng/mL，the risk of cancelled IUI cycles was increased，becasues of multi-follicular developing and absence of dominant follicle development.

In order to remove the artifacts of peripheral digital subtraction angiography (DSA), an affine transformation-based automatic image registration algorithm is introduced here. The whole process is described as follows: First, rectangle feature templates are constructed with their centers of the extracted Harris corners in the mask, and motion vectors of the central feature points are estimated using template matching technology with the similarity measure of maximum histogram energy. And then the optimal parameters of the affine transformation are calculated with the matrix singular value decomposition (SVD) method. Finally, bilinear intensity interpolation is taken to the mask according to the specific affine transformation. More than 30 peripheral DSA registrations are performed with the presented algorithm, and as the result, moving artifacts of the images are removed with sub-pixel precision, and the time consumption is less enough to satisfy the clinical requirements. Experimental results show the efficiency and robustness of the algorithm.
Algorithms ; Angiography, Digital Subtraction ; methods ; Artifacts ; Automation ; methods ; Image Processing, Computer-Assisted ; methods

Anesthesiology in China is transforming from traditional anesthesia only to perioperative medicine. Perioperative medicine has a more comprehensive idea from the perspective of being patient- oriented ，regarding comfort and aponia，rapid recovery and good prognosis of patients as the goal. It also requires individualized perioperative treatment scheme. Since organ protection is a critical issue in perioperative medicine ，patients benefit from maintaining favorable organ function and preventing failure induced by injury. Organ transplantation will become one of the hotspots in future medicine with the development of surgery. Liver transplantation ，as the main type of transplantation ，causes multiple organ dysfunctions during the perioperative period. It is an urgent and key point of perioperative medicine to illustrate its mechanisms and establish relevant protections.

Objective To investigate the influence of chemotherapy combined with autophagy inhibitor on apoptosis and calreticulin (CRT) expression on colonic cancer cells. Methods The colon cancer cells HCT116 were taken as the target in the present study. The inhibition rates (IC50) of chemotherapeutics oxaliplatin, 5-Fu and SN-38 were assessed by MTT assay. The changes in CRT expression on the membrane of HCT116 and apoptosis were determined with flow cytometry before and after treatment with chemotherapeutics. CRT location in HCT116 was detected by fluorescent immunoassay before and after treatment with chemotherapeutic agents. The influence on HCT116 autophagy was determined by Western blotting after treatment with these chemotherapeutic agents. The changes in CRT expression on HCT116 membrane and apoptosis were determined with flow cytometry before and after treatment with the chemotherapeutics combined with autophagy inhibitor chloroquine (CQ). Results The ratio of apoptosis and membrane expression of CRT were elevated 12 hours after treatment with Oxaliplatin, 5-Fu and SN- 38, but without statistical significance. Fluorescent immunoassay showed a transposition of CRT from cytoplasm to the membrane after oxaliplatin treatment. Western blotting revealed that oxaliplatin, 5Fu and SN38 induced autophagy of HCT116 cells, and the autophagy was inhibited by the addition of CQ. Flow cytometric analysis indicated that the percentages of annexin V+ cells and membrane expression of CRT were higher after treatment with the chemotherapy agents combined with CQ. The upregulation of CRT expression on membrane was obviously higher after treatment with oxaliplatin combined with CQ than that before the treatment with these agents (P=0.027). Conclusion Oxaliplatin combined with CQmay increase the apoptosis rate of HCT116 cells and upregulate CRT expression in the membrane.

【Objective】 To investigate the effects of different temperature on ¹⁸F-fluoro-deoxyglucose(¹⁸F-FDG) uptake in brown adipose tissue(BAT) of tumor mice with use of Micro-PET/CT imaging. 【Methods】 After fasted for 8 h, all the four BALB/c breast cancer model mice were successively exposed to 3 different temperature together at 30℃, 25℃ and 20℃ respectively. Under each exposure, the mice were injected intravenously with ¹⁸F-FDG and 45 min later imaged by micro PET/CT. Then the images were reviewed and the maximum standardized uptake values(SUVmax) of BAT in the deep neck and scapula were measured. 【Results】 At the temperature of 30 ℃, 25 ℃ and 20 ℃, the SUVmax of ¹⁸F-FDG uptake in BAT were 0.63±0.16, 1.29±0.17 and 2.21±0.11, respectively; in tumor tissues 2.36±0.11, 1.85±0.10 and 1.51±0.17, respectively; in brain tissues 2.35±0.19, 2.34±0.14 and 2.38±0.11, respectively. 【Conclusion】 At room temperature(20℃), the metabolism in BAT is significantly enhanced, the metabolism in tumor tissues is induced. When temperature increases to 25℃ or 30℃, the uptake in non-specific areas such as brown fat in mice is significantly reduced or eliminated, and the metabolism in tumor tissues is enhanced. Temperature has significant effects on ¹⁸F-FDG uptake of BAT in mice and temperature control can help better ¹⁸F-FDG Micro-PET/CT imaging for tumor mice.

【Objective】The aim of this study was to investigate the proportion of dominant follicles（PDF）on HCG day in young and aged women.【Methods】In total，3 064 cycles of GnRH agonist long protocol and 918 cycles of GnRH antagonist protocol from 2014 to 2016 were retrospectively included. Patients were divided into 3 groups（low PDF，< 20%； medium PDF，≥20% and ≤40% ；high PDF，> 40%）. The measurements regarding the ovarian stimulation characteris? tics ，clinical pregnancy rate was compared between different PDF groups stratified by age and protocol. 【Results】 In patients aged ≤30 years who received the GnRH agonist long protocol，no significant difference was found in the clinical pregnancy rate between the different PDF groups（P > 0.05）. Regarding the GnRH antagonist protocol，no significant difference was found in the clinical pregnancy rate and moderate or severe OHSS rate（P > 0.05）. In patients aged ≥40 years， the clinical pregnancy rate in the low PDF group was higher than that in the high PDF groups（40% vs. 30.88%）in GnRH agonist long protocol. Concerning the GnRH antagonist protocol，patients in the low PDF group had a significantly higher clinical pregnancy rate than those in the other two groups（27.27% vs. 9.09% ，7.40% ；P = 0.002）.【Conclusions】PDF within 20% is recommended for older patients（≥40 years），especially in those receiving the GnRH antagonist protocol.

OBJECTIVEThis study was aimed to investigate the effects of carbon monoxide releasing molecule (CORM-2), a novel carbon monoxide carrier, on the reendothelialization of carotid artery in rat endothelial denudation model.

METHODSMale rats subjected to carotid artery balloon injury were treated with CORM-2, inactive CORM-2 (iCORM-2) or dimethyl sulfoxide (DMSO). The reendothelialization capacity was evaluated by Evans Blue dye and the immunostaining with anti-CD31 antibody. The number of circulating endothelial progenitor cells (EPCs) was detected by flow cytometry. The proliferation, migration, and adhesion of human umbilical vein endothelial cells (HUVECs) were assessed by using [3H]thymidine, Boyden chamber and human fibronectin respectively. The expressions of protein were detected by using western blot analysis.

RESULTSCORM-2 remarkably accelerated the re-endothelialization 5 d later and inhibited neointima formation 28 d later. In addition, the number of peripheral EPCs significantly increased in CORM-2-treated rats than that in iCORM-2 or DMSO-treated rats after 5 d later. In vitro experiments, CORM-2 significantly enhanced the proliferation, migration and adhesion of HUVECs. The levels of Akt, eNOS phosphorylation, and NO generation in HUVECs were also much higher in CORM-2 treated group. Blocking of PI3K/Akt/eNOS signaling pathway markedly suppressed the enhanced migration and adhesion of HUVECs induced by CORM-2.

CONCLUSIONCORM-2 could promote endothelial repair, and inhibit neointima formation after carotid artery balloon injury, which might be associated with the function changes of HUVECs regulated by PI3K/Akt/eNOS pathway.

Animals ; Carbon Monoxide ; metabolism ; pharmacology ; Carotid Artery Injuries ; drug therapy ; immunology ; metabolism ; pathology ; Carotid Artery, Common ; drug effects ; immunology ; metabolism ; pathology ; Cell Adhesion ; drug effects ; Disease Models, Animal ; Endothelial Cells ; drug effects ; immunology ; metabolism ; pathology ; Endothelium, Vascular ; drug effects ; metabolism ; pathology ; Humans ; Male ; Rats ; Rats, Sprague-Dawley