2.ISOLATION AND IDENTIFICATION OF XJ STRAIN OF CANINE DISTEMPER VIRUS
Jun QIAO ; Qing-Ling MENG ; Xian-Zhu XIA ; Hong-Bin HE ; Quan-Shui FAN ;
Microbiology 1992;0(01):-
A canine distemper virus strain was isolated from the lung of dog coming from Aksu in Xing Jiang using lung primary M cell during the CDV molecular epidemiological study. It was demonstrated to be a virulent strain of CDV by a series of systematic identification such as morphology , serology neutralization test, canine infection test, and molecular virology test.
3.Transdermal delivery of diclofenac sodium gel after iontophoresis
Jian-Qing GAO ; Hua WANG ; Wen-Quan LIANG ; Ming-Xia CHEN
Journal of Zhejiang University. Medical sciences 2002;31(6):437-439
OBJECTIVE: To observe the in vivo effect of combined iontophoresis and laurocapram pretreatment on transdermal delivery of diclofenac sodium gel. METHODS: Diclofenac sodium gel was prepared using polyvinyl alcohol, carboxymethylcellulose sodium and hydroxypropylmethyl cellulose. The diclofenac blood level in rabbits was measured in four groups: passive diffusion, laurocapram pretreatment, iontophoresis (current density controlled at 0.3 mA/cm(2)) and combined laurocapram pretreatment and iontophoresis. Rabbit stratum corneum of each of the four groups was examined using a scanning electron microscope. RESULTS: Diclofenac blood concentration in the passive diffusion group was undetectable. The diclofenac blood concentration area under the curve compared with time was 8.4 &mgr;l ml(-1) h(-1) in the laurocapram pretreatment group, 2.7 &mgr;l ml(-1) h(-1) in the iontophoresis group and 15.4 &mgr;l ml(-1) h(-1) in the combination group. There was no detectable damage observed by scanning electron microscopyto the stratum corneum after iontophoresis or laurocapram pretreatment. CONCLUSION: The combination of iontophoresis and laurocapram pretreatment appears to enhance transdermal delivery of diclofenac sodium gel wi thout significant skin damage.
4.The protective effects of IPC on isolated myocardial ischemia/reperfusion injury in rats.
Xia CAO ; Xin-quan GU ; Shi-jie YANG ; Hui-qing ZHANG
Chinese Journal of Applied Physiology 2003;19(2):114-116
AIMTo investigate the protective effects and mechanism of IPC on myocardial ischemia/reperfusion injury.
METHODSEffects of IPC on arrhythmia and coronary blood flow and the release of AST, CPK, LDH, SOD and LFO at different time after ischemia/reperfusion injury in rat Langendorff hearts were studied.
RESULTSIPC decreased the release of AST, CPK and LDH and increased myocardial SOD activity and decreased LPO level. IPC also inhibited ischemia/reperfusion arrhythmias and increased coronary blood flow.
CONCLUSIONThe results showed that IPC had well protective effects on myocardial ischemia/reperfusion injury.
Animals ; Female ; Heart ; physiopathology ; Ischemic Preconditioning, Myocardial ; methods ; Male ; Myocardial Reperfusion Injury ; prevention & control ; Rats ; Rats, Wistar
5.Prelimilary experimental study of manganese enhanced-functional MR imaging on cat model about acute epilepsy caused by pentylenetetrazol
Jin-Bai HUANG ; Hai-Bo XU ; Xiang-Quan KONG ; Ding-Xi LIU ; Qing-Xia KONG ; Sheng-Gang SUN ;
Chinese Journal of Radiology 2001;0(01):-
Objective To explore the activated brain region of acute epilepsy in cat model induced by pentylenetetrazol(FFZ)with manganese enhanced-functional MR imaging(ME-fMRI),and evaluate the application of ME-fMRI on localization of the activated brain.Methods Forty cats were divided into 4 groups by random number table method as epileptic A and B groups as well as control A and B groups. Cats of epileptic groups were injected with PTZ(55 mg/kg)intramuscularly,and those of control groups were injected with the saline at same dose.The behavior change in the epileptic and control group A was observed and electroencephalogram(EEG)was also undertaken.Cats of epileptic and control group B were performed ME-fMRI,and the percentage of the enhanced signal intensity was then calculated.Results After injection with PTZ(55 mg/kg)intramuscularly,epileptic seizure was all evoked,and then EEG recording showed spike-wave and polyspike-wave complexes.The neocortex of cats of epileptic group B was diffusely phanero-enhanced on ME-fMRI.The percent enhancement of signal intensity in cortex of frontal lobe,parietal lobe and occipital lobe was(34.6?5.7)% and that in cortex of temporal lobe with(22.9? 6.5)%,whereas those of control group B with(14.9?4.5)% and(11.6?3.2)% respectively.And there was significant difference between the above different localization of the brain in the two groups (t=-10.43,-5.46 respectively,P
6.Effects and Safety of Hemoperfusion Therapy on Infants with Tetramine Poisoning
de-xuan, WANG ; rui-xia, LIN ; qing, YANG ; guo-quan, PAN ; chun-xue, YAN ; ling-wei, JIN
Journal of Applied Clinical Pediatrics 1992;0(06):-
Objective To explore the effects and safety of hemoperfusion(HP) therapy on tetramine poisoning in infants.Methods Thirty-five infants with tetramine poisoning were divided into two groups: HP group(n=18) and non HP group(n=17).The changes of blood tetramine concentration and clinical symptom improving of both groups after the treatment were observed together with the adverse effects of HP group.Results The average blood tetramine concentration of HP group was higher than that of non HP group(342.2?333.4 vs 117.9?50.8 ?g/L,P
7.Percutaneous vertebroplasty in treatment of compression fracture of thoracic and lumbar vertebrae
Kai ZHANG ; Fan HE ; Zeng-Hui WU ; Qing-Shui YIN ; Hong XIA ; Ri QUAN ; Yunbing CHANG ; Shun-hai CAO
Chinese Journal of Trauma 1993;0(06):-
Objective To discuss the value and experience of the percutaneous vertebroplasty (PVP)in the treatment of vertebral body compression fracture(VCF)in aged osteoperosis.Methods PVP was performed in 44 cases with VCF including 28 with single vertebral compressed fracture,12 with double compressed fracture and four with triple compressed fracture,with 67 vertebrae,for clinical and radiologieal evaluation.Results The mean follow-up was 15 months(4-23 months).There could be seen immediate relief of pain in 40 cases,out-of-bed activities at operation day in 19 and out-of-bed activ- ities at second day after operation in 25.Postoperative X-ray showed uniformly distributed bone cement in the vertebral,without leakage.Conclusion PVP is a recommendable method for VCF,for it has ad- vantages of pain relief,vertebrae stabilization,minimal invasion and minor complications.
8.Analysis on the decline of hepatitis C virus RNA in the blood using a mathematical model.
Zu-Jiang YU ; Quan-Cheng KAN ; Yun HE ; He-Qing JIANG ; Hong-Xia LIANG ; Tai-Sheng LI
Chinese Journal of Hepatology 2007;15(12):886-888
OBJECTIVETo establish a mathematical model of hepatitis C virus (HCV) replication and develop a working theory for antiviral therapy in order to understand the dynamics of HCV replication.
METHODSPeripheral blood cells of 4 hepatitis C patients were cultured. Quantities of the HCV were detected every 15 min by real-time PCR. The data were analyzed using SPSS software. A mathematical functional relationship between HCV RNA and the time lapse was established.
RESULTSThe quantity of HCV RNA declined and it fell into a mathematical model: Y=3E+0.8e(-0.5467x) (r=0.9547). The estimated virion half-life was 45 min on the average.
CONCLUSIONSThe decline of HCV RNA in the blood is not of a linear trend and the HCV RNA lasts a longer time although the speed of the decline is faster than that in vivo.
Adult ; Half-Life ; Hepacivirus ; Hepatitis C, Chronic ; blood ; virology ; Humans ; Models, Theoretical ; Nonlinear Dynamics ; RNA, Viral ; blood ; Viral Load ; Virus Replication
10.Role of connective tissue growth factor (CTGF) in proliferation and migration of pancreatic cancer cells.
Yu-chun BAI ; Quan KANG ; Qing LUO ; Dao-qi WU ; Wei-xia YE ; Xue-mei LIN ; Yong ZHAO
Chinese Journal of Oncology 2011;33(10):732-736
OBJECTIVETo explore the expression of connective tissue growth factor (CTGF) in pancreatic cancer and its influence on the proliferation and migration of cancer cells.
METHODSThe expression of CTGF in pancreatic cell line PANC-1 cells was analyzed by real-time PCR and in pancreatic carcinoma (50 cases) tissues by immunohistochemistry. The ability of proliferation and migration in vitro of PANC-1 cells was tested by MTT assay, scratch test and Boyden chamber test after the CTGF gene was overexpressed by Ad5-CTGF or silenced with Ad5-siCTGF transfection.
RESULTSCTGF was overexpressed in both pancreatic cancer cells and tissues. Overxpression of CTGF leads to increased proliferation and migration of PANC-1 cells. The CTGF-transfected PANC-1 cells showed apparent stronger proliferation ability and scratch-repair ability than that of empty vector controls. The results of Boyden chamber test showed that there were 34 cells/field (200× magnificantion) of the CTGF-transfected overexpressing cells, much more than the 11 cells/field of the empty vector control cells; and 6 cells/microscopic field of the Ad5-siCTGF-transfected silenced cells, much less than the 15 cells/field of the control cells.
CONCLUSIONSCTGF is overexpressed in both pancreatic cancer cells in vitro and in vivo, indicating that it may play an important role in the cell proliferation and migration in pancreatic cancer.
Adenoviridae ; genetics ; Cell Line, Tumor ; Cell Movement ; Cell Proliferation ; Connective Tissue Growth Factor ; genetics ; metabolism ; Humans ; Pancreatic Neoplasms ; metabolism ; pathology ; Recombinant Proteins ; genetics ; metabolism ; Transfection