Objective To discuss the treatment of cervical cancer in different stages.Methods The treat- ment of 42 cases of cervical cancer in this hospital from January 2000 to December 2005 was analysed,and clinical treatment modalities were analysed too.Results(1)A total of 6 cases of uterine carcinoma in situ received hysterec- tomy plus bilateral salpingo-oophorectomy.(2)A total of 26 cases was inⅠb-Ⅱa stage,of which 15 cases with cer- vical cancer were treated with a simple radical hysterectomy;6 cases of cervical cancer were radiotherapied after radi- cal hysterectomy;7 cases of cervical cancer received radical hysterectomy plus radiotherapy.(3)A total of 10ⅡbⅣstage eases selected radiotherapy and chemotherapy.Conclusion Different treatment should be applied in differ- ent stages of cervical cancer,and the choice for treatment is important for patients' quality of life after treatment. Comprehensive treatment can improve the survival of patients with cervical carcinoma.

Objective To propose a new classification scheme of otitis media (OM). Methods By reviewing current internat ional classification systems of OM, we analyzed their disadvantages and the prog ress made in histopathologic studies of OM, then worked out a new classification scheme to apply it to clinic. Results In current internati onal classification systems of OM, those apparent clinical signs are regarded as main standards of classification. Their main disadvantage is that these signs c annot very well indicate the condition of pathologic and functional changes of i mportant hearing structure areas. We proposed a “Three-stage Scheme” of OM cl assification and applied in 1 160 cases of OM. The accuracy rate of diagnosis w as 82.4% in 348 cases of early-stage OM, 98.4% in 696 cases of chronic OM, and 95.2% in 116 cases of OM sequela. Conclusion The “Three-s tage Scheme” system is scientific, simple and practical. Its accuracy rate of d iagnosis is very high. It plays a guiding role in diagnosis and treatment of OM.

Microbial selective side-chain degradation of phytosterol,which can obtain the steroid medicine intermediate compounds-androst-4-ene-3,17-dione (4-AD) and androsta-1,4-diene-3,17-dione (ADD),has an important meaning to pharmacy. There is no systematic literature concerned in existence. Its mechnism,approaches,influencing factors and so on over these years were fully reviewed in the paper. The trend of development in the area is expanded.

Objective To study percutaneous absorption kinetics and synergistic penetration enhancement of tetrahydropalmatine,aconitine and ginsenoside Rg1.Methods The in vitro intact and stripped skin permeation experiments were carried out by using Permcell KH-5P horizontal proliferation system.The percutaneous kinetic parameters of tetrahydropalmatine,aconitine and ginsenoside Rg1 were determined by HPLC,and the transdermal behaviors of the three active ingredients and interactions of combination were investigated.Result The cumulative amounts in 24 h of intact skin of tetrahydropalmatine,aconitine and ginsenoside Rg1 were 123.95,50.32,96.25(?10-10 cm2/s).The cumulative amounts in 24 h of stripped skin of them were 539.00,93.60,354.70(?10-10 cm2/s).The LogP were 1.66,0.66 and 0.57 respectively.Each component had cumulated amount permeated of improvement by 64%～293% in coexistence system of three active ingredients.Conclusion The combination of three active ingredients results in a synergistic enhancement effect on percutaneous absorption of every active ingredient,and it is shown that the maximal enhancement effect appears between aconitine and ginsenoside Rg1.

Objective To describe the work of quality control for lipids measurement in CNHS 2002.Methods The quality control was applied over the whole project including staff training,making standard operating procedure(blood collection,separation,temporary storage,transportation,final storage),lab materials assignment,internal quality control in central lab and participating US Disease Control Center's Lipids Standard Program(LSP)as external quality control.Results Seven times of national training programs were held and 326 technicians from CDCs in 31 provinces,autonomous regions, municiplalities and 132 surveyed counties received related trainings.During the program,321 persons passed examination (98.5%)and among them 205 got the A score(62.9%);The field work was implemented strictly according to the procedure. Three automatic biochemical instruments were used in the measurement and there is no significant difference between means of serum TC,TG and HDL-C from several batches of quality control serum.Parallel measurements were conducted in five percent randomly selected samples,the ratio of bias less than 3% was 99.05%,96.40% and 98.30% for TC,TG and HLD-C, respectively.Compared with means of LSP control sera,the results showed that all bias was less than 5% except one bias of TG result(7.02%)in one batch of LSP controls.Conclusion The work of quality control in this survey guaranteed the accuracy of plasma lipids measurement,and provided the basic data for the epidemiological description of dyslipidemia status among Chinese and the further analysis.

ObjectiveTo evaluate the effects of selective cyclooxygenase-2 inhibitor nimesulide lone and combined with cisplatin on tumor growth,and Ki67 and Caspase-3 expression in lung cancer xenografts in nude mice. Methods The mice were randomly divided into 4 groups:the control group,the nimesulide group,the cisplatin group and the nimesulide combined with cisplatin group. A549 cells were injected into BALB/c nude mice subcutaneously.On the 21st day after treatment tumor tissues were collected,and the xenografts growth were observed. The expression of Ki67 and Caspase-3 were detected by immunohistochemical method.ResultsNimesulide combined with cisplatin could significantly inhibited the xenografts growth compared with nimesulide or cisplatin.The tumor inhibition rate was 44.33％ in the nimesulide group,53.61％ in the cisplatin group and 80.41％ in the nimesulide combined with cisplatin group (P ＜ 0.05).Immunohistochemical analysis showed that the expression rates of Caspase-3 was significantly increased in the nimesulide combined with cisplatin group (67.43 ± 23.57 ) ％,the cisplatin group (48.40 ± 20.37 ) ％,and the nimesulide group (38.65 ± 15.37)％,compared with the control group (27.63 ± 13.03)％ (P ＜ 0.05).The expression rate of Caspase-3 was significantly increased in the nimesulide combined with cisplatin group compared with the nimesulide group or the cisplatin group (P ＜ 0.05 ).The expression rate of Ki67 was significantly decreased in the nimesulide group combined with cisplatin group (24.34 ± 15.90)％,the cisplatin group (40.85 ± 22.47)％ and the nimesulide group (53.33 ± 19.67)％ compared with the control group ( 80.43 ± 16.88 ) ％ ( P ＜ 0.05 ).The expression of Ki67 was significantly decreased in the nimesulide combined with cisplatin group compared with the nimesulide group or the cisplatin group ( P ＜ 0.05 ).Conclusion Nimesulide can inhibit human lung cancer A549 cells xenografts in nude mice growth,Nimesulide enhanced the inhibitory effects of cisplatin.The mechanism may be related to inhibition of tumor cell Ki-67 expression,increased expression of Caspase-3,inhibition of tumor cell proliferation,and inducing tumor cell apoptosis.

Cystatin C (Cys C) is used to evaluate the glomerular filtration rate (GFR), renal function and thus reflects changes in endogenous marker. Cys C is not affected by gender, muscle mass, malignancy and its production rate is usually constant, making it one of ideal markes of GFR. This article focuses on clinical application and research of Cys C in a variety of renal diseases, especially in renal dysfunction at an early stage of clinical significance.

Invasion and metastasis are critical determinants on cancer morbidity.Genes and molecules participating in these steps have been demonstrated as potential prognostic factors.Growth factors and their receptors,cell-cycle regulators,cell-adhesion molecules and m trix-degrading enzymes are potentially used as prognostic factors.However,increased understandings for molecular mechanisms underlying carcinogenesis and its implementation in the treatment of gastric cancers have recently been focused on the development and incorporation of targeted agents with chemotherapy.

Objective To evaluate the value of serum tumor markers in diagnosis of over 80 years old patients with colorectal cancer.Methods Serum CA199,CA242 and CEA of 68 cases senile patients with colorectal cancer and 35 cases senile patients with benign large intestinal diseases were detected by ELISA.Results Serum levels of CA199,CA242 and CEA in patients with colorectal cancer were (37.95 ± 15.95 ) U/ml,( 50.08 ± 24.20 ) U/ml,( 22.34 ± 17.35 ) μg/L respectively and were significantly higher than that in patients with benign large intestinal diseases CA199 ( 10.73 ± 6.27 ) U/ml,CA242 ( 8.00 ± 3.52 ) U/ml,CEA(2.32 ± 1.01 )μg/L.In addition,the level of these three markers was increased with the progress of colorectal cancer and the sensitivity of diagnosis was related with the stages of colorectal cancer.Parallel combined testing methods increased the diagnostic sensitivity to 83.6％,and serial combined testing increased the diagnostic specificity to 88.7 ％.Conclusions Serum tumor markers showed certain values in the diagnosis of the senile patients with colorectal carcinoma.Furthermore,the combined determination of tumor markers is helpful for early diagnosis of colorectal cancer and increases the sensitivity and specificity in diagnosing of colorectal carcinoma.

Objective To analyze the demand for home care service and its influential factors among elderly in Tianjin city.Methods A total of 254 residents aged 60 and above from Tianjin city were investigated with a self-designed questionnaireDemand for Home Care Service,Katz index of independence in Activities of Daily Living(ADL) and Lawton Instrumental Activities of Daily Living (IADL) scale.Results The mean score for home care service has been revealed (2.40±0.67),as the ranking demonstrated:public welfare (3.61±1.04),social participation (2.69±1.32),medical service (2.56±0.90),rights safeguard by law (2.37±1.54),mental need (2.26±0.99),and need for daily life care (1.73±0.69).Multiple linear regression analysis showed that income,education level,activity of daily living and the convenience degree of seeking treatment were major factors affecting demand for home care service among elderly,which explained 16.3％ of the variance.Conclusions Oriented by demand,further progress shall be achieved multidimensionally,under which circumstances,individualized service ought to be de-livered.Simultaneously,the vulnerable elderly groups require more attention.