1.Clinical control study in methods of pre-labor pubic preparation
Hongyu ZHANG ; Yueqing HAN ; Xiaohuan WANG ; Qing WANG ; Min HUANG
Chinese Journal of Practical Nursing 2008;24(21):1-2
Objective To determine whether it is necessary to execute pre-labor pubic shaving.Methods Women(336 cases)who received pubic shaving through vaginal delivery from 2003 to 2006 were set as the control group.Women(103 cases)who were waiting for delivery currently in our hospital were set as the test group.The test group only received perineal cleaning without pubic shaving.If episiotomy was needed during the operation,the perineal hair Was cut by scissors.The perineum infectious rate and postpartal infection Was compared in the two groups.We interviewed the patients attitude for pubic shaving and counted the numing time used for pubic shaving.Results The perineal infection in the test group was 5.8%,which was no statistically different from that of the control group(2.4%),U=0.33,P>0.05.Totally 95% women in the test group support this method demonstrated humanization.In the telephone folow-up visit for patients in the control group they comphined itch and discomfort during the regeneration of pubes.The average numing time for pubic shaving Was(4.6±0.6)minutes.Conclusions No pubic shaving for pre-labor skin preparation was feasible.If operation was disturbed by pubes we could use scissors to cut it short.This method could reduce the skin infection and propagation of blood-derived infectious disease.It increased the comfort of parturient,saved time and proved to be a nursing method of humanization.
2.Expression of Interleukin-15 in Peripheral Blood of Patients with Mycoplasma Pneumoniae Infection and Bronchitic Asthma Attacking
yu, ZHAO ; lan-fang, CAO ; min, MA ; han-qing, YU
Journal of Applied Clinical Pediatrics 2006;0(22):-
0.05).Conclusion The increasing of IL-15 in peripheral blood after MP infection may play a role in bronchitic asthma pathogenesis.
3.Efficacy of water knife needle release combined with bone peptide injection for heel pain.
Wen-zhi CHEN ; Qing-liang SHEN ; Hui-min WANG ; Han-qing WU
Journal of Southern Medical University 2010;30(8):1953-1955
OBJECTIVETo evaluate the efficacy of water knife needle release combined with bone peptide injection in the management of heel pain.
METHODSThirty-five patients with unilateral heel pain were treated with water knife needle release and bone peptide injection under local anesthesia. The deep tissue with the tenderness was released in the operation, and the result was evaluated 1 week after the surgery to decide whether to conduct another surgery. No more than 3 treatment sessions were administered. The efficacy was evaluated according to nimodipine method by the principles of Chinese clinical drug guidance, and the complications of the surgery were observed.
RESULTSSix months after the surgery, 28 cases had excellent results, 3 had good outcomes, 2 showed improvement, and 2 failed to respond favorably, with a rate of good and excellent result of 94.2%. No adverse side effect was recorded in the follow up of the patients.
CONCLUSIONWater needle knife release combined with bone peptide injection can produce a good result in the treatment of heel pain.
Adult ; Aged ; Female ; Foot Diseases ; therapy ; Heel ; Humans ; Injections ; Male ; Middle Aged ; Needles
4.A clinical study of the characteristics of the cervical lymph node metastasis in patients with salivary gland adenoid cystic carcinoma
Qing HUANG ; Nannan HAN ; Shengwen LIU ; Lizhen WANG ; Min RUAN ; Wenjun YANG ; Chenping ZHANG
Chinese Journal of Clinical Oncology 2016;43(24):1094-1098
Objective:Adenoid cystic carcinoma (ACC) is an uncommon malignant neoplasm, which mostly originates from the major and minor salivary glands of the head and neck region. This study aims to provide new information on head and neck ACC with cervical lymph node metastasis. Methods:Out of the 616 patients who underwent primary tumor resection from 1995 to 2008 in the authors' hospital, 62 cases with cervical lymph node metastasis were analyzed. Results:The general incidence rate of cervical lymph node me-tastasis in ACC was approximately 10%. The base of the tongue, mobile tongue, and mouth floor were the most frequent sites of lymph node metastasis with incidence rates of 19.2%, 17.6%, and 15.3%, respectively. Most cases exhibited the classictunnel-stylemetastatic pattern of occurrence, and the levelⅠb andⅡregions were the most frequently involved areas. Primary site and lympho-vascular invasions were significantly associated with lymph node metastasis. High patient mortality rate was also significantly correlat-ed with a high number of lymph node positive cases. Conclusion:Cervical lymph node metastasis has a high tendency of occurrence in the tongue-mouth floor complex, following the classictunnel-stylemetastatic pattern. Peritumoral lymphovascular invasion could be taken as a strong predictor for the occurrence of lymph node metastasis, which ultimately leads to poor prognosis of ACC patients. A selective neck dissection should be considered as a management in such patients.
5.Expression of cell cycle regulator ATM, Chk2 and p53 and their clinicopathological correlation in breast infiltrative ductal carcinoma.
Xiao-juan PEI ; Qing-xu YANG ; Shao-jie LIU ; Min SU ; Zhuo-ya HUANG ; An-jia HAN
Chinese Journal of Pathology 2012;41(7):479-480
Ataxia Telangiectasia Mutated Proteins
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Breast
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metabolism
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pathology
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Breast Neoplasms
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metabolism
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pathology
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Carcinoma, Ductal, Breast
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metabolism
;
pathology
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Carcinoma, Intraductal, Noninfiltrating
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metabolism
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pathology
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Cell Cycle Proteins
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metabolism
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Checkpoint Kinase 2
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DNA-Binding Proteins
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metabolism
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Female
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Humans
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Lymphatic Metastasis
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Neoplasm Grading
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Protein-Serine-Threonine Kinases
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metabolism
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Tumor Burden
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Tumor Suppressor Protein p53
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metabolism
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Tumor Suppressor Proteins
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metabolism
6.Determination of phenylethanoid glycosides in Orobanche coerulescens.
Guo-qing HAN ; Cai-feng LI ; Xiao-qin WANG ; Min-hui LI ; Jing LI
China Journal of Chinese Materia Medica 2015;40(21):4218-4222
Orobanche caerulescens is an important medicinal resource in Orobanchaceae. The present study aims to establish methods for determination of acteoside, crenatoside, and total phenylethanoid glycosides in O. caerulescens, and determine the content in 15 samples to evaluate the resource utilization of this medicinal plant. The content of acteoside and crenatoside were quantitatively determined by HPLC, while total phenylpropanoid glycosides was estimated by UV-VIS spectrophotometry. According to the results, the content of acteoside was the highest in O. caerulescens, followed by crenatoside. The contents of acteoside, crenatoside, and total phenylethanoid glycosides were between 1.15% - 15.60%, 0.83% - 4.47%, and 6.78% - 27.43%, respectively, which had significant differences. The acquisition time has great influence on the content of main components of O. caerulescens. The content of phenylethanoid glycosides is higher in the samples which were collected at the flowering stage. The two determination methods were proved to be simple, accurate and reliable, and can be used to evaluate the quality and resource utilization of O. caerulescens.
Chromatography, High Pressure Liquid
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methods
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Drugs, Chinese Herbal
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analysis
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Glycosides
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analysis
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Orobanche
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chemistry
7.Cloning, prokaryotic expression, and functional identification of a sesquiterpene synthase gene (AsSS4) from Aquilaria sinensis.
Liang LIANG ; Qing-Mei GUO ; Zheng ZHANG ; Yan-Hong XU ; Xiao-Min HAN ; Juan LIU
Acta Pharmaceutica Sinica 2014;49(12):1724-1729
A sesquiterpene synthase (AsSS4) full-length open reading frame (ORF) cDNA was cloned from wounded stems of Aquilaria sinensis by RT-PCR method. The result showed that the ORF of AsSS4 was 1,698 bp encoding 565 amino acids. Prokaryotic expression vector pET28a-AsSS4 was constructed and transformed into E. coli BL21 (DE3) pLysS. Recombinant AsSS4 protein was obtained after induction by IPTG and SDS-PAGE analysis with a MW of 64 kD. Enzymatic reactions using farnesyl pyrophosphate showed that recombinant AsSS4 protein purified by Ni-agarose gel yielded five sesquiterpene compounds, cyclohexane, 1-ethenyl-1-methyl-2, 4-bis(1-methylethenyl)-, β-elemene, α-guaiene, α-caryophyllene and δ-guaiene. This paper reported the first cloning and functional characterization of AsSS4 gene from A. sinensis, which will establish a foundation for future studies on the molecular mechanisms of wound-induce agarwood formation in A. sinensis
Alkyl and Aryl Transferases
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biosynthesis
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genetics
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Azulenes
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Cloning, Molecular
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DNA, Complementary
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Escherichia coli
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Open Reading Frames
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Polyisoprenyl Phosphates
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Recombinant Proteins
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biosynthesis
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Sesquiterpenes
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metabolism
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Sesquiterpenes, Guaiane
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Thymelaeaceae
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enzymology
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genetics
8.Analysis on first- and second-line drug resistant patterns in 518 Mycobarterium tuberculosis strains in Shanghai
Yidian LIU ; Shenjie TANG ; Qing ZHANG ; Lingfie JING ; Min HAN ; Jun YUE
Chinese Journal of Infectious Diseases 2011;29(9):544-548
Objective To investigate the resistant patterns of Mycobacterium tuberculosis (MTB) strains against first- and second-line anti-tuberculosis drugs. Methods Drug susceptibility tests of 518 MTB strains collected from January 2008 to March 2009 were performed using BactecMGIT 960. The data were analyzed by chi square test. ResultsIn 518 strains, 168 (32.44%) were all sensitive to all seven drugs, 350 (67.56%) were resistant to at least one drug. Among all strains, 72 (13.90%) were resistant to one drug, 24 (4.63%) were resistant to two drugs, 254 (49.03%) were resistant to three or more drugs. A total of 217 strains (41.89 %) were classified as multi-drug resistant tuberculosis (MDR-TB)strains and 65(12.55%)were extensively drug resistant tuberculosis (XDR-TB) strains which accounted for 29.95 % of MDR-TB strains. The drug resistant rate of isoniazid which belonged to first-line drugs was 53.67% (278 strains) and that of ofloxacin which belonged to second-line drugs was 39.77 % (206 strains). In 433 retreated patients, the drug resistant rate against any drugs, MDR rate and XDR rate were 72.05%, 46.42% and 13.86%,respectively, which were all higher than those in treatment naive patients (44.70%, 18. 82% and 5.88%, respectively; x2 = 24. 253, x2 = 22. 229 and x2 = 4. 117, respectively; all P < 0.01).ConclusionsThe resistant rate of MTB is high in a tuberculosis specialized hospital in Shanghai, and MDR-TB also shares a high resistant rate as well as XDR-TB. Furthermore, drug resistance is more common in retreated patients.
9.Expression of nitric oxide and γ -aminobutyric acid in the retina of two kinds of amblyopia cats
Han-Min, WANG ; Ao, RONG ; Li-Juan, MO ; Qing-Song, LI ; Xing-Ru, ZHANG
International Eye Science 2016;16(11):2006-2009
AIM: To study the role of nitric oxide ( NO ) and γ-aminobutyric acid ( GABA) in the formation of amblyopia by establishing 2 different types of amblyopic models.METHODS:A total of 18 aged 3-week kittens were randomly divided into monocular deprivation, strabismus and normal groups. All types of amblyopia were developed in the experimental eyes that were detected by P-VEP 12wk later. The cats were killed and the immunocytochemistry staining method were applied to observe under the light microscope the changes of distribution and positive cells areas of NO and GABA across the amblyopic retinal, compared to that from the normal cats of identical age.
RESULTS: The P-VEP showed that the amplitude of wave P1 was lower (P<0. 05) and the P1 latent time was longer ( P<0. 05 ) in two types of amblyopic cats than those in the normal cats. Compared to the normal cats, the NO and GABA positive cells areas were obviously reduced ( P<0. 05 ) across the retina in the amblyopic cats. But no significant difference was found between two kinds of amblyopic cats.
CONCLUSION:The NO and GABA play an important role in the formation of amblyopia in the level of retinal.
10.Effect of Cx43 on proliferation of C6 glioma cells and its mechanisms
Yeru LI ; Xiangmei PANG ; Min LI ; Qing WANG ; Yuqing YANG ; Xiaojian HAN ; Liping JIANG
Chinese Pharmacological Bulletin 2017;33(7):1008-1013
Aim To explore the effect of Cx43 over-expression on proliferation of C6 cells and its mechanisms by transfecting pCMV-Cx43cDNA plasmid into C6 cells.Methods pCMV-Cx43cDNA plasmid was transfected into C6 cells by liposome to up-regulate the expression of Cx43, and C6 cells with over-expression of Cx43 was stably cloned by using G418.Determination of cell doubling time and soft agar colony formation assay to detect the degree of cell proliferation.The cells were treated with ERK1/2 specific blocker PD98059(30 μmol·L-1) and p38MAPK specific blocker SB202190(10 μmol·L-1)respectively, the expression of Cx43, p-Cx43, p-ERK1/2 and p-p38MAPK of each group were detected by Western blot, and the activity of each group was detected by MTT Assay.Results pCMV-Cx43cDNA plasmid was transfected into C6 cells successfully.Cell lines with over-expression Cx43(C6-Cx43) or empty vector (C6-pCMV) were stably selected by using G418.Determination of cell doubling time and soft agar colony formation experiments showed that the proliferative rate and the colony number of C6-Cx43 group were significantly decreased, compared with that of C6 group and C6-pCMV group(P<0.01);ERK1/2, p38MAPK specific blockers were treated with each group,Western blot showed that the expression of Cx43 protein was increased(P<0.01), while p-Cx43 protein was decreased (P<0.05) in C6-Cx43+PD98059 group and C6-Cx43+SB202190 group,compared with that of C6-Cx43 group.Conclusion Cx43 may decrease the proliferation of glioma cells through ERK1/2, p38MAPK pathways.