Objective To build a normal value of serum sex hormone in the healthy children in favour of the diagnosis of children precocious puberty and the evaluation of the growth of the children.Method Serum of 462 healthy children(aged from 1 month to 14 years;205 females,257 males)was collected,and the level of testosterone(T) and 17-estradiol(E2).Results There were no significant deviation in the levels of E2 of deferent genders between 1-12 months,but the level of T in boys and girls were obviously different.The puberty of boys was la-ter than girls.There was no significant deviation in the level of E2 of the boys in different periods,but the levels of T in boys at different ages were obviously different.Conclusions With the improvement the detection methods,the normal values of the serum sex hormone in healthy children have been changing.It is helpful for diagnosis of children precocious puberty by rebuilding normal values of T and E2 .

Objective To study the effect of hypothyroidism on oxidative stress status in developing rat brain and to further explore the mechanism of impaired brain development caused by hypothyroidism. Methods Perinatal hypothyroidism was induced by administering propylthiouracil(PTU) solution to the dams by gavage.The oxidative stress indexes were measured in brain homogenate of normal and hypothyroid pups which were sacrificed on the 21st d after birth. Results As compared to the control,the following indexes were found to be increased in the hypothyroid group: protein carbonyl contents,thiobarbital acid reactive substances,reduced glutathione,total antioxidative capacity,activities of superoxide dismutase and glutathione peroxidase(P0.05). Conclusion Hypothyroidism during rat brain development may cause oxidative stress,which may be related to the brain damage caused by hypothyroidism.

Adolescent ; Asthma ; blood ; drug therapy ; Case-Control Studies ; Child ; Child, Preschool ; Female ; Humans ; Interleukin-17 ; blood ; Male

Adult ; Female ; Humans ; Male ; Middle Aged ; Parathyroid Neoplasms ; diagnostic imaging ; surgery ; Retrospective Studies ; Ultrasonography

BACKGROUND:Currently, conventional treatment methods for heart failure are al ineffective. OBJECTIVE:To explore the combined effects of human umbilical cord stem cel s and CD133+cel s in mice with heart failure, providing a new insight into the treatment of heart failure. METHODS:Ful-term newborn umbilical cord from vaginal delivery was col ected to isolate CD133+cel s and human umbilical cord stem cel s using lymphocyte separation medium method. Twenty Balb/C nude mice were randomly subjected to mononuclear cel injection (mononuclear cel group) or injection of CD133+cel s combined with human umbilical cord stem cel s (combined group) via the tail vein after establishing heart failure models. RESULTS AND CONCLUSION:Fourteen days after injection, the body weight and liver, heart and lung mass of mice were significantly larger in the combined group than the mononuclear cel group (P<0.05). After 30 days, myocardial cel s arranged regularly in the combined group, but disorderly in the mononuclear cel group;compared with the mononuclear cel group, the average area of myocardial col agen fibers was significantly decreased in the combined group (P<0.05), and the level of serum matrix metal oproteinase-9 was also significantly lower in the combined group (P<0.05). Masson staining showed that blue-stained col agen fibers in the combined group were less but arranged neatly;however, in the mononuclear cel group, the number of col agen fibers that arranged irregularly was increased to different extents. To conclude, the combined use of CD133+cel s and human umbilical cord stem cel s has desired outcomes in the treatment of heart failure in mice, indicating a higher clinical value.

OBJECTIVE:To establish the quality standard of Bikemin capsule to control the quality METHODS:TLC method was used for the identification of Ephedra sinica and the detection of limitation of aconitine in Bikemin capsule Ephedrine and pseudoephedrine in the capsule were determined by HPLC on C18 column with the detection wavelength at 215nm RESULTS:The standard curves of ephedrine and pseudoephedrine were linear in the ranges of 0 02～0 10mg/ml (r=0 9 993) and 0 01～0 05mg/ml(r=0 9 996)respectively The average recoveries were (101 4?1 78)% for ephedrine and (98 08?1 81)% for pseudoephedrine CONCLUSION:The identification method is specific and the determination method is sensitive The quality standard is suitable for control of quality of Bikemin capsule

Objectives To observe the changes of magnetic resonance imaging(MRI) during the course of treatment in hepatolen-ticular degeneration (HLD) and to evaluate its related factors.Methods Sixty - three patients with HLD received routine SE sequence MRI of brain and liver.Twenty - two patients with abnormal MRI in 42 patients and each of them had 2-4 times of serial MRI ex-aminations at interval of 1.0- 1.5 year.Results Ten patients had lesion in both brain and liver in 22 patients. Nine patients only in brain, 3 patients in liver.The major lesions disappear or decrease after rational treatments. There were only slight or no changes of MRI abnormalities in patients receiving treatments or age of patients was over 15 year old. Abnormal MRI findings shown again after stopped treatments over 2 months. In such cases, the clinical and MRI improvements were slow.The rate of clinical and MRI improvement in patients with adequate treatment were markedly higher than patients with inadequate treatment.Conclusions Liver and brain lesion will show in MRI disappear after adequate treatment;There are only slight or no change or showing again in MRI abnormalities in pa-tients with inadequate treatments compared with poor clinical improvement. MRI is beneficial to understanding therapeutic effect and prognosis of HLD.

Objective To observe the effect and security of lovasatin treating hyperlipidemia in children with steroid resistance nephrotic syndrome(SRNS).Methods Thirty-seven children with SRNS were administered lovasatin with normal liver function before lipid-lowing treatment.The changes of plasma lipids [total cholesterol(TC),trigly ceride(TG)] and lipoprotins [low density lipoprotein(LDL),very low density lipoprotein(VLDL),high density lipoprotein(HDL)],albumin(Alb),serum creatinine(Scr),aminotransferase(ALT),24 hours of urinary protein and drug side-effects were observated for 4 weeks.All children treated with regular glucocorticoids therapy for 2 months still presented with urinary protein significant positive.Results There were decreasing of plasma lipids and lipoproteins after 2 weeks of lovasatin treatment,especially for TG,24 hours of urinary protein(Pa

0.05).Conclusions The results by GH exercise test is identified with GH provocative test.Because the GH exercise test is safe and simple,it is appropriate to use the GH exercisetest to screen for growth hormone deficiency during childhood.

Objective To investigate the effects of benazepril on the expression of receptors for advanced glycation end-products(RAGE)in diabetic rat kidneys,and to explore its mechanisms of renal protection in diabetic rats. Methods Thirty SD rats were randomly divided into three groups(n=10 in each group): normal control group,diabetic control group and diabetic with benazepril group.Blood glucose,blood lipid,HbA1c,kidney to body weight ratio and 24 h urinary protein excretion were detected after 12 weeks.RAGE mRNA level was analyzed by quantitative RT-PCR. Results Compared with the normal control group,the blood glucose,HbA1c,triglyceride,total cholesterol and low density lipoprotein cholesterol in the diabetic control group and diabetic with benazepril group were significantly increased(P0.05).The kidney to body weight ratio,24 h urinary protein excretion and RAGE mRNA level in the diabetic with benazepril group were significantly higher than those in the normal control group(P