1.Can hormone replacement therapy be used following treatment for gynecologic malignancies?.
Yong-liang GAO ; Jian-qing ZHU
Chinese Journal of Oncology 2012;34(9):719-720
Adenocarcinoma
;
therapy
;
Breast Neoplasms
;
therapy
;
Carcinoma, Endometrioid
;
therapy
;
Carcinoma, Squamous Cell
;
therapy
;
Cystadenocarcinoma, Serous
;
therapy
;
Endometrial Neoplasms
;
therapy
;
Female
;
Genital Neoplasms, Female
;
therapy
;
Hormone Replacement Therapy
;
Humans
;
Ovarian Neoplasms
;
therapy
;
Treatment Outcome
;
Uterine Cervical Neoplasms
;
therapy
2.Optimization of extraction technology of the polysaccharides from sporophyll of Undaria pinnatifida Suringar by orthogonal design
Liang ZHU ; Qing ZHANG ; Qinchang ZHU ; Yifei WANG ; Yinzhou CEN
Chinese Journal of Marine Drugs 2000;0(06):-
Objective To study the extraction technology of the polysaccharides from Sporophyll of Undaria pinnatifida Suringar and find out the optimum extraction condition.Methods The extraction technology was studied by the orthogonal design.Results The optimum extraction conditions were to add 50 times of water to the Undaria pinnatifida Suringar material at 90℃ for 4 hours.Conclusion This optimum extraction technology based on the orthogonal design was confirmed by the experiment and was available in the future research.
3.Role of the E gene in the infectivity of SINV and SINL in infected cells
Lihua WANG ; Shihang FU ; Wuyang ZHU ; Qing TANG ; Guodong LIANG
Chinese Journal of Microbiology and Immunology 2010;30(5):410-415
Objective To elucidate the molecular basis on the differences of infectivity in infected cells between Sindbis virus(SINV:YN87448 virus)and Sindbis like virus(SINLV:XJ-160 virus).Methods Compare the E(glycoprotein)gene sequence and secondary structure of YN87448 virus and XJ-160 virus by bioinformatics analysis.Analyze the contribution of E gene to the biological differences between SINV and SINLV by constructing recombinant virus.Results By bioinformatics analysis,YN87448 virus and XJ-160 virus have the same genomic structure,which has 11 717 nt and 11 626 nt respectively.There are 82 amino acid differences between E gene of these two viruses,and showed scattered distribution.The main peak is basically the same for the hydrophobic of the E gene protein,but in some region existing small differences.The recombinant virus which exchanged the E gene of XJ-160 virus with YN87448 virus totally showed the biological character of YN87448 virus,either in the showing time of CPE,plaque forming time and plaque diameter,or in expression of functional proteins.Conclusion E gene plays a major role in the differences of infectivity in infected cells between SINV and SINLV,this result provide the molecular biological evidences for elucidating the biological differences between SINV and SINLV.
5.A mucosal immune cells homing and infection of HIV.
Liang-zhu LI ; Jian-qing XU ; Xiao-yan ZHANG
Chinese Journal of Virology 2010;26(3):260-264
6.Effect of Integrated Pharmaceutical Care on Rational Drug Use in Patients Treated with Ambulatory Chemotherapy
Liang TAO ; Bin ZHU ; Zheyuan WANG ; Bo YU ; Qing ZHAI
China Pharmacist 2014;(10):1729-1731
Objective:To promote the rational drug use in the patients treated with ambulatory chemotherapy. Methods:A model of integrated pharmaceutical care was established using such service as the real-time prescription examination, configuration standardi-zation, active care and prescription review. Results:The integrated pharmaceutical care could effectively reduce the unreasonable drug use, optimize drug use structure, lower the medical expenses and provide supporting data for clinical decision-making. Conclusion:The development of integrated pharmaceutical care in the patients treated with ambulatory chemotherapy is an important field for phar-macists displaying professional skills, which can promote the rational drug use. The treatment efficacy and life quality of cancer patients can be improved with the cooperation of doctors, nurses and pharmacists.
7.Advances in Brainstem Infarction
Dan ZHU ; Chun-Xiao YANG ; Qing-Cheng LIANG ;
International Journal of Cerebrovascular Diseases 2006;0(11):-
Brainstem infarction accounts for about 9% to 21.9% of all cerebral infarctions. This article reviews the etiology of brainstem infarction and its pathogenesis,clinical manifestation,diagnosis,and treatment.
8.The bi-pedicled gastrocnemias myocutaneous flap:its anatomic basis and clinical application
Yong-Qing XU ; Jun YANG ; Yue-Liang ZHU ;
Chinese Journal of Orthopaedic Trauma 2004;0(12):-
Objective To provide a clinical anatomic basis for the bi-pedicled gastrocnemius myocuta- neous flap which is to be used to repair defects of the Achilles tendon and posterior skin of the ankle.Methods In anatomic study,out of 30 cadaver specimens,bi-pedicled V-Y gastrocnemius myoeutaneous flaps were harvested to measure the downward sliding lengths of the flap when the knee was at flexion of different degrees.In clinic,12 patients with the Achilles tendon and posterior ankle skin defects were repaired with the bi-pedicled gastrocnemius myocutaneous flaps.The areas of composite defects ranged from 10 cm?6 cm to 6 cm?4 cm.They were followed up for four months to 12 years.Results The maximal sliding length of the flap reached (9.2?0.9)cm when the knee was at flexion of 90?.All the clinical flaps survived and ambulation of the patients recovered.The dorsiflexion and plantarflexion of the ankles reached 11.0??1.4?and 35.0??4.6?respectively.Conclusion This flap is suitable for one-stage repair of composite Achilles tendon defects.
9.Determination of polysaccharide from Ecklonia kurome
Liang ZHU ; Qing ZHANG ; Yifei WANG ; Yanyan KANG ; Yinzhou CENG
Chinese Journal of Marine Drugs 2000;0(06):-
Objective To develop a stable and simple method to measure the polysaccharide in Ecklonia kurome Okam.Methods After the conversion coefficient of polysaccharide from Ecklonia kurome Okam to glucose was obtained,the content of polysaccharide was measured by phenol-sulfuric acid method.Results The correlation coefficient of standard curve was(0.9998),the recovery rate was 97.35% and RSD was 4.68%.Conclusion The method was convenient for content determination of polysaccharide from Ecklonia kurome Okam as well as its related products.
10.Inhibition effect of captopril and losartan on expression of matrix metalloproteinase-1,-9 induced by angiotensin Ⅱ in rat vascular smooth muscle cells
Qing ZHU ; Gaowa SARREN ; Xin YUE ; Xuguo LIANG
Chinese Journal of Geriatrics 2011;30(4):337-341
Objective To investigate the effect of angiotensin converting enzyme inhibitor (ACEI) captopril and angiotensin Ⅱ receptor antagonist losartan on the mRNA expression of matrix metalloproteinase (MMP)-1 and MMP-9 in vascular smooth muscle cells induced by angiotensin Ⅱ (Ang Ⅱ ).Methods Male Wistar rats' thoracic aortic vascular smooth muscle cells were cultured in vitro.The cultured cells were divided in to control group,Ang Ⅱ group,captopril group,losartan group,and captopril plus losartan group.Cells in all groups were collected at the culture end-point.MMP-1 and MMP-9 mRNA expressions were detected by RT-PCR method in the collected specimens,and the effects of Ang Ⅱ on MMP-1 and MMP-9 mRNA expression and the intervention effects of captopril and losartan were observed in different Ang Ⅱ concentrations and different action times to vascular smooth muscle cells.Results ( 1 ) MMP-1 mRNA expression gradually increased along with the increments of Ang Ⅱ concentration and the action time (P<0.05),and the most significant concentration was 10-6 mol/L (P<0.01).(2)Captopril (5 × 10-6 mol/L) and losartan (5 × 10-6mol/L) inhibited the action of AngⅡ (P<0.05,P<0.01).MMP-9 mRNA expression was 0.47±0.03 ,0.86 ± 0.04,0.94±0.14 and 1.12±0.19 vs.0.10±0.04 (P<0.05,P<0.01) respectively when Ang Ⅱ concentration was 10-7 ,10-6 ,10-5 and 10-4 mol/L respectively.Captopril (5 × 10-6mol/L) and losartan (5 × 10-6 mol/I) significantly inhibited the MMP-9 mRNA expression which was stimulated by Ang Ⅱ (P<0.05,P<0.01),especially in captopril plus losartan group.The MMP-9 mRNA expression increased with the prolonging of stimulating time of Ang Ⅱ,MMP-9 mRNA expression was earlier than that of MMP-1.Conclusions AngⅡ increases the expression of MMP-1 and MMP-9 of vascular smooth muscle cells in a dose-and time-dependence manner.Captopril and losartan inhibit the MMP-1 and MMP-9 mRNA expression of vascular smooth muscle cells induced by Ang Ⅱ ,and the inhibition is the strongest when losartan was combined with captopril.The inhibitive effects is positively correlated to action time.