Objective To study the efficacy and mechanism of sequential therapy of clopidogrel combined with aspirin in the treatment of transient ischemic attack.Methods 150 cases with transient ischemic attack were set as the research subjects.According to the random number table method,they were divided into aspirin group,clopidogrel group and combination group.The clopidogrel group was treated with 75mg clopidogrel,once daily.The aspirin group was treated with 50mg aspirin,twice a day.The combined group was treated with 300mg clopidogrel +300mg aspirin at the first day,75mg clopidogrel +100mg aspirin at the second day.Three groups were treated for a period of 15 days.After 15d treatment,the raw fiber protein (FIB),two D dimer (D-D),activated partial thromboplastin time (APTT) and prothrombin time (PT) of three groups and adverse reactions,curative effect and recurrence rate were analyzed.Results After treatment,the FIB,D-D,APTT and PT of the combined group were (3.14±0.24)g/L,(0.61±0.26)mg/L,(33.58±2.77)s,(15.67±0.72)s,which of the clopidogrel group were (3.32±0.25)g/L,(0.91±0.32)mg/L,(30.19±2.19)s,(15.64±0.78)s,which of the aspirin group were (3.40±0.22)g/L,(1.02±0.39)mg/L,(29.71±2.21)s,(13.21±0.80)s,which were significantly improved than before treatment[the combination group (3.59±0.26)g/L,(2.50±0.35)mg/L,(27.53±2.41)s,(11.23±0.69)s;the clopidogrel group (3.58±0.28)g/L,(2.51±0.40)mg/L,(27.19±2.53)s,(11.73±0.82)s;the aspirin group (3.61±0.27)g/L,(2.46±0.32)mg/L,(27.61±2.13)s,(11.68±0.77)s,the effect of the combined group was better than that of the clopidogrel group and aspirin group (F=8.12,P<0.05).The total effective rate of the combination group (96.00%) was significantly higher than that of clopidogrel group (84.00%) and aspirin group (76.00%),the differences were statistically significant (x2 combination group vs.clopidogrel group=4.00,x2combination group vs.aspirin group=8.31,all P<0.05).The adverse reactions in the three groups had no difference,but the recurrence rate of the combination group (8.00%) was significantly lower than that of the clopidogrel group (24.00%) and the aspirin group (28.00%),the differences were statistically significant (x2combination group vs.clopidogrel group=4.76,x2combination group vs.aspirin group=6.78,all P<0.05).Conclusion The combination of clopidogrel and aspirin in the treatment of transient ischemic attack has significant effect and low recurrence rate,which is worthy of popularizing widely.

Objective To investigate the effect of nerve growth factor in the treatment of senile cerebral hemorrhage and its effect on the nerve function.Methods According to the digital table,90 elderly patients with cerebral hemorrhage were randomly divided into observation group and control group,45 patients in each group.The control group was given conventional treatment for cerebral hemorrhage.The observation group was injected mouse nerve growth factor based on the control group.The efficacy of the two groups after treatment,nerve function defect score(NIHSS) before and after treatment changes and simple type Fugl-Meyer motor function score(FMA) were compared.Results The total effective rate of the observation group after treatment was 93.33%,which was higher than 73.33% of the control group,the difference was statistically significant(χ2=6.480,P=0.010).Before treatment,the NIHSS scores of the two groups had no statistically significant difference (t=0.333,P=0.739).After treatment,the NIHSS score of the observation group was (6.49±7.02)points,which was lower than (11.01±8.65)points of the control group,the difference was statistically significant(t=2.721,P=0.007).Before treatment,the FMA scores of the two groups had no statistically significant difference (t=0.198,P=0.198).Compared with the control group,the FMA score of the observation group after treatment was significantly higher,the difference was statistically significant(t=3.222,P=0.001).Conclusion Rat nerve growth factor in the treatment of elderly patients with cerebral hemorrhage can effectively improve the patients' neurological deficits and activities of daily living,the clinical efficacy is significant,it is worthy of clinical application.

Adenoma, Pleomorphic ; complications ; Adult ; Humans ; Male ; Pharyngeal Neoplasms ; complications ; Sleep Apnea, Obstructive ; etiology

Objective To explore the preventive and therapeutic role of silencing type Ⅰ rat platelet-binding protein motifs depolymerization protein-like metalloproteinase 2(ADAMTS2)by siRNA on experimental liver fibrosis in vitro.By studying the mechanism of siRNA silencing of ADAMTS2,we also aim to evaluate the feasibility of ADAMTS2 as a target for anti-liver fibrosis therapy.Methods Three pairs of siRNAs targeting ADAMTS2 mRNA 2237,2597 and 690 targets were designed and synthesized by utilizing RNA design software.The most effective siRNA was chosen to transfect HSC-T6 cell line to test the tendency of hepatic stellate cell(HSC)activation and ex pression of ADAMTS2,COL1α1,COL(I),α-SMA,TGF-β1,MMP-2 and TIMP-3.These were quantified using real time-PCR,Western blotting,and MTT assays.Results Of the same dosage and time of injection,siRNA 2237 inhibited ADAMTS2 gene expression significantly more than other siRNAs.siRNA-ADAMTS2 2237 markedly inhibited ADAMTS2 gene and protein expression of HSCT6 with more than 80％ efficiency.Conversely,siRNA-ADAMTS2 2237 markedly reduced the gene and protein expressions of COL(I),α-SMA and TGF-β1 on HSC-T6 and inhibited the proliferation of HSC.Conclusions siRNA-ADAMTS2 2237 could effectively knockdown the gene and protein expression of ADAMTS2 in HSC-T6 cell lines.Silencing ADAMTS2 by siRNA significantly inhibited the activation,proliferation of HSC and the gene and protein expressions of COL(I),α SMA,and TGF-β1,and it may have a potential anti-fibrotic effect.ADAMTS2 might be an efficient target for anti-fibrotic therapy.

Objective To investigate the clinical characteristics of skin lesions of tsutsugamushi disease.Methods The skin lesions and clinical characteristics of 17 hospitalized patients with tsutsugamushi disease were analyzed. Results Of the patients, 88.2% (15/17) developed eschar and skin ulcer, 64.7% (11/17) had skin rashes. The patients often developed solitary, painless and nonpruritic eschar 4 to 10 days after the occurrence of fever, with indefinite locations and average diameter of 1.2 cm (range: 0.5 - 3.0 cm). The eschar was covered with a black and dry surface and surrounded by a ring-shaped erythematous halo, and usually shedded 5 to 9 days after the appearance with the formation of shallow ulcer. Superficial lymphadenectasis was observed near the eschar in 80% of the patients with eschar, and no eschar was observed at the first visit in 66.7% of the patients. Skin rashes mainly included nonpruritic congestive papules (45.5%) and maculopapular rashes (54.5%), and often subsided 2 to 5 days after emergence. Conclusions The skin lesions of tsutsugamushi disease include eschar and skin rashes. Eschar is an important characteristic and highly suggestive of this entity.

Objective To investigate the effect of hexosamine biosynthesis pathway on the development of insulin resistance induced by high fat diet.Methods Normal male SD rats were randomly divided into three groups:control(fed with normal chow),high fat(fed with high fat diet for 13 weeks),and rosiglitazone (intragastric administration with rosiglitazone for 5 weeks)groups.After 13 weeks,all the rats were sacrificed,serum and muscle triglycerides(TG),serum total cholesterol(TC),and serum and muscle free fatty acids(FFA) were measured.Insulin sensitivity wss evaluated by insulin sensitivity index(ISI)and glucose infused rat(GIR) with the hyperinsulinemic englycemic clamp technique.The flux of HBP in skeletal muscle was detected with the expression level of glutamine-fructose-6-phosphate transaminase(GFAT)mRNA(RT-PCR),the content of UDPGlcNAc(HPLC)and the level of O-GlcNAc glycosylation in skeletal muscle proteins(Western blot). Results Compared with control group,senlm TG,TC,FFA and muscle TG,FFA levels of high fat group increased(aII P<0.01).both ISI and GIR decreased(both P<0.01),and the leveIs of GFAT mRNA(0.51±0.05 vs 0.18±0.02),UDP-GlcNAc[(6.18±0.86 vs 2.42±0.36)nmol/g],and O-GIcNAc glycosylation of skeletal muscle proteins in high fat group were raised(all P<0.01).In rosiglitazone group,serum and muscle TG.FFA welc deceased(all P<0.01).insulin sensitivity was increased(P<0.05)and the flux of HBP[GFAT mRNA 0.27±0.03,UDP-GIcNAc(2.62±0.32)nmol/g]was reduced(all P<0.05)as compared with high fat group. Conclusions High fat diet-induced insulin resistance in rats is correlated with the increased flux of HBP in skeletaI muscle.which is decreased by rosiglitazone.

Objective To observe the learning and memory ability,oxidative stress,apoptosis morphological changes in the hippocampus,and to explore the effects of hyperuricemia on cognitive function.Methods 51 healthy male SD rats were randomly divided into three groups (17 in each group):Blank group,Distilled water group and Hyperuricemia group.Using the lavage methods of yeast extract combined with ethambntol to establish hyperuricemic model.Morris water maze test was used to measure the learning and memory ability.The levels of MDA,GSH-Px,ASAFR,SOD were measured through chemical colorimetry.Hippocampus morphology structures were observed under the HE staining light microscopy to detect the apoptosis of hippocampus cone cell with TUNEL.Results The average escaped latency and passing platform times of Blank group had no significant difference compared with those of Distilled water group and Hyperuricemia group (all P＞ 0.05).GSH-Px,ASAFR,SOD of Hyperuricemia group ((83.70 ± 5.47) nmol/mg,(606.03±46.61) U/L and (55.05 ± 2.11) units/mg) were increased compared with those of Blank group ((67.28±8.37) nmol/mg,(473.84 ± 57.64) U/L,(45.79 ± 2.05) units/mg) and Distilled water group ((71.96±9.47) nmol/mg,(505.97 ± 47.19) U/L,(46.24 ± 3.65) units/mg) (all P＜ 0.05).Compared with Blank group ((3.19±1.14) μmol/L) and Distilled water group ((3.16±1.43) μmol/L),the MDA of Hyperuricemia group ((1.74±0.45) μmol/L) was significantly decreased (all P＜ 0.05).Form and structures of hippocampal neurons of each group were basically normal under the HE staining light microscopy.Compared with Blank group (CA1:(3.59±0.63) ％,CA3:(5.54± 0.78) ％) and Distilled water group (CA1:(3.25±0.97) ％,CA3:(5.96± 0.82) ％),the hippocampal cells of Hyperuricemia group (CA1:(4.04± 0.78) ％,CA3:(5.95±0.80) ％) also had no statistical differences (P＞0.05).Conclusion Hyperuricemia has antioxidant effect on hippocampal neurons and has no effect on cognitive function and hippocampal neural morphology in rats.

Adult ; Aged ; Carcinoma, Squamous Cell ; metabolism ; pathology ; Female ; Glucose Transporter Type 1 ; metabolism ; Glucose Transporter Type 3 ; metabolism ; Head and Neck Neoplasms ; metabolism ; pathology ; Humans ; Lymphatic Metastasis ; Male ; Middle Aged

Adult ; Angiofibroma ; Humans ; Male ; Nose Diseases ; Turbinates ; pathology

Humans ; Laryngeal Neoplasms ; Male ; Middle Aged ; Neoplasms, Muscle Tissue