1.Preservation of anterior capsule during vitrectomy and lensectomy
Qing, WANG ; Wen-Yi, ZHANG ; Rui-Hua, MENG ; Ju, KANG
International Eye Science 2006;6(5):992-994
AIM: To evaluate the preservation of anterior capsule during vitrectomy and lensectomy.ment (RD) and grade C proliferative vitreoretinopathy (PVR)underwent pars plana vitrectomy (PPV) and pars plana lensectomy (PPL) with preservation and polishing of the anterior capsule. Of the 15 eyes, 4 eyes had giant tear, 3 had recurrent rhegmatogenous retinal detachment (RRD), 2 had diabetic retinopathy. Totally 6 eyes had gas and 9 had silicone oil tamponade. The surgeries were evaluated according to the visual acuity (VA) and the postoperative complications during the follow-up of at least 3 months.in all eyes, improved by 3± 3 lines overall. Eight eyes were implanted posterior chamber intraocular lens (PCIOL) successfully at 2-3 months after operation, including 6 having gas and 2 having silicone oil tamponade. No eyes had central anterior capsule opacity, corneal decompensation, puplillary block, retina redetachment or other complications.an intact anterior capsule in eyes with RD and PVR. Preserving the anterior capsule can help preventing intraoperative and postoperative complications of gas or silicone oil, simplify future PCIOL placement, and maintaining a normal iris appearance.
2. Inheritance and development: A statement of establishment of chinese cardiopulmonary resuscitation technology
Medical Journal of Chinese People's Liberation Army 2015;40(9):693-698
This paper describes the ideas of the Chinese technology of cardiopulmonary resuscitation (CPR). The authors believe that its strategy should be based on inheritance of both the essence of CPR technology of both indigenous and western origin, and it should take into consideration of three factors: time, place and population in order to establish a composite mathematical and theoretical foundation of the CPR technology, and to form a guideline of CPR, and eventually establish the CPR system with Chinese characteristic.
3.Liquisolid technique for enhancement of dissolution prosperities of tanshinone II(A).
Xiao-qian LIU ; Qing-ju MENG ; Xue-lin XU ; Jie ZHAO ; Hua YANG ; Hong YI
China Journal of Chinese Materia Medica 2015;40(24):4840-4846
The technique of liquisolid compress is a new technique developed in 1990s, which was considered to be the most promising technique to improve the dissolution of water-insoluble drugs. In this article, tanshinone II(A) and the extracts of the ester-solubility fractions were chosen as the model drugs to evaluate the effects of the liquisolid technique for enhancement of dissolution properties of tanshinone II(A). Several liquisolid tablets (LS) formulations containing different dosage of drugs and various liquid vehicle were pre-pared and for all the formulations, microcrystalline cellulose and silica were chosen as the carrier and coating materials to evaluate their flow properties, such as angle of repose, Carr's compressibility index and Hausner's ratio. The interaction between drug and excipients in prepared LS compacts were studied by differential scanning calorimetry(DSC) and X-ray powder diffraction (XRPD). The dissolution curves of tanshinone II(A) from liquisolid compacts were investigated to determine the technique's effect in improving the dissolution of tanshinone II(A) and its impacting factors. According to the results, the dissolution increased with the rise in the dissolution of the liquid-phase solvent. The R-value and drug dosage can significantly affect the drug release, but with less impact on active fractions. This indicated that liquisolid technique is a promising alternative for improvement of dissolution property of water-soluble drugs, and can make a synergistic effect with other ester-soluble constituents and bettern improve the release of tanshinone II(A). Therefore, the technique of liquisolid compress will have a better development prospect in traditional Chinese medicines.
Calorimetry, Differential Scanning
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Diterpenes, Abietane
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chemistry
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Solubility
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X-Ray Diffraction
4.Prominent smooth muscle differentiation in fibroadenoma of breast: report of a case.
Jiong SHI ; Hong-yan WU ; Yi-fen ZHANG ; Fan-qing MENG ; Xiang-shan FAN
Chinese Journal of Pathology 2011;40(9):636-637
Actins
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metabolism
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Adult
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Breast Neoplasms
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metabolism
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pathology
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surgery
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Calcium-Binding Proteins
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metabolism
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Cell Differentiation
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Desmin
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metabolism
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Diagnosis, Differential
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Female
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Fibroadenoma
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metabolism
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pathology
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surgery
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Hamartoma
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pathology
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Humans
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Hyperplasia
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Leiomyoma
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pathology
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Microfilament Proteins
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metabolism
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Muscle, Smooth
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pathology
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Phyllodes Tumor
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pathology
5.Some Approaches for the Selective Isolation of Rare Actinomycetes
Wei XIAO ; Ming-Gang LI ; Xiao-Long CUI ; Yi-Qing LI ; Meng-Liang WEN ;
Microbiology 1992;0(01):-
The focus of microbiologists has moved to the rare actinomycetes.For selective isolation of rare actinomycetes that all play the important role in bioactive compounds,the approaches which involve the methods using gellan gum and flooding solution、 rehydration-centrifugation(RC)、 extremely high frequency radiation(EHF)、 bacteriophage and sucrose-gradient centrifugation were introduced in this paper.
6.Establishment of the Experimental Curriculum Teaching System for Long Educational System Students
Hong YU ; Wen-Qing ZHANG ; Shou-Yi DING ; Rui LV ; Lin MENG ;
Microbiology 1992;0(01):-
In view of the predominance problems in present experimental teaching of medical microbiology, we reformed the experimental contents and experimental teaching methods,to establish an entire experimental curriculum teaching system to be suitable for seven-year system and long educational system students,which manifests the creative teaching idea.
7.Value of combined detection of GP73 and alpha-fetoprotein in diagonosis of hepatocellular carcinoma.
Qiao-Ping WU ; Fu-Yi XIE ; Qing-Ling MENG ; Yin-Yu MU
Chinese Journal of Experimental and Clinical Virology 2012;26(2):148-149
OBJECTIVETo explore the diagnostic value of the measurement of serum Golgi protein 73 (GP73) in the diagnosis of hepatocellular carcinoma (HCC).
METHODSEnzyme-linked immunosorbent assay (ELISA) was used to detect the serum GP73 in the 81 cases of HCC, 71 cases of chronic hepatitis or cirrhosis (CH/LC) and 65 cases of healthy blood donors, and to evaluate the sensitivity and specificity in the diagnosis of HCC through the ROC curves.
RESULTSThe average levels of serum GP73 in HCC, CH/LC and Normal groups were (152.67 +/- 33.59) ng/ml, (93.15 +/- 20.02) ng/ml and (58.95 +/- 17.29) ng/ml(o) After calculating through the ROC curves, 120 ng/ml was set as the optimal cut-off point, GP73 has a sensitivity of 77.80% and a specificity of 78.00%.
CONCLUSIONSGP73 as a serum marker in the diagnosis of HCC had a higher sensitivity than AFP, and the combined detection of GP73 and AFP could improve HCC diagnosis.
Carcinoma, Hepatocellular ; diagnosis ; Enzyme-Linked Immunosorbent Assay ; Female ; Humans ; Liver Cirrhosis ; blood ; Liver Neoplasms ; diagnosis ; Male ; Membrane Proteins ; blood ; alpha-Fetoproteins ; analysis
8.Study on syndrome quantification, differentiation and classification of traditional Chinese medicine with data envelopment analysis.
Qing-Bo MENG ; Yi-Xin YIN ; De-Zheng ZHANG ; Guo-Ping YANG
China Journal of Chinese Materia Medica 2013;38(10):1631-1642
To raise the syndrome sequence quantification, differentiation and classification algorithm based on data envelopment analysis for solving the modeling issue of syndrome differentiation and classification of traditional Chinese medicine (TCM). This algorithm has three steps: first, in order to obtain basic units for explaining pathogenesis, and establish a syndrome collection on this basis mechanisms of syndrome differentiation and classification were analyzed and classified according to TCM theory, mechanisms of syndrome differentiation and classification were analyzed and classified according to TCM theory; second, regularity and syndromes of corresponding prescriptions were sought according to the incidence and development progress of syndromes, and mathematical tools of data envelopment analysis were used to calculate state data of syndromes in each stage and obtain quantitative syndrome sequence; finally, syndrome sequence was taken as the measurement standard to quantify candidate syndromes and diagnostic information, and the similarity was calculated to obtain the matching degree between diagnostic information and candidate syndromes, so as to complete the syndrome differentiation and classification calculation. According to the results of model-based reasoning, the algorithm could indicate the regularity implied in prescription materials, and grasp the dynamic process of syndromes in an all-round way, and its results were verified through calculation and analysis on clinical cases. At least, it provides an idea for quantitative modeling of TCM.
Data Mining
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Diagnosis, Differential
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Drugs, Chinese Herbal
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therapeutic use
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Humans
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Medicine, Chinese Traditional
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Models, Theoretical
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Phytotherapy
9.Effects of perindopril at different doses on cardiac function and ACE2/Ang-(1-9)/Ang-(1-7) axis of ischemic cardiac dysfunction rabbits
Xiao HAO ; Shuren LI ; Tiantian MENG ; Qing GAO ; Yi DANG ; Liying XUN ; Kexin YUAN ; Qianhui ZHANG ; Qingqing HAO ; Xiaoyong QI
Chinese Journal of Pathophysiology 2016;32(3):554-557,563
[ ABSTRACT] AIM:To investigate the different dose of perindopril on cardiac function in the rabbits with ische-mic cardiac dysfunction .METHODS:Male rabbits weighing 2.5~3.0 kg ( n=30) were randomly divided into 3 groups (n=10):high dose perindopril group (HD group), low dose perindopril group (LD group) and cardiac dysfunction group (CD group).The Left anterior descending coronary artery of the rabbits was ligatured for model preparation .In HD group, the rabbits were treated with perindopril split normal saline solution (1 g/L)2 mL· kg-1 · d-1 .In LD group, the rabbits were treated with perindopril split normal saline solution (0.33 g/L)2 mL· kg -1 · d-1.In CD group, the rabbits were treated with normal saline solution 2 mL· kg-1 · d-1 .Four weeks after treatment , the cardiac function was measured via echocardiography , the mRNA expression of angiotensin-converting enzyme 2 ( ACE2 ) and angiotensin type 2 receptor (AT2R) was analyzed by real-time PCR, serum angiotensin (Ang)-(1-9) and Ang-(1-7) levels were detected by ELISA. RESULTS:Compared with CD group , the cardiac function of the 2 groups treated with perindopril was significantly im-proved (P<0.01), and more improvement in HD group was observed than LD group (P<0.05).The serum angiotensin ( Ang)-(1-9) and Ang-(1-7) level and the mRNA expression of ACE 2 and AT2R in the 2 groups treated with perindopril were significantly improved (P<0.01).Compared with LD group, the mRNA expression of ACE2 and AT2R and the ser-um levels of Ang-(1-9) in HD group were significant improved (P<0.05), while no difference of serum Ang-(1-7) level was observed.Correlation analysis revealed that the improvement of the cardiac function was associated with serum Ang -(1-9) level, mRNA expression of ACE2 and AT2R (P<0.01), but has no significant correlation with serum Ang-(1-7) lev-el.CONCLUSION:High dose of perindopril may improve more cardiac function in ischemic cardiac dysfunction model in rabbits.The mechanism may relate to increasing serum Ang-(1-7) level to activate AT2R.
10.Cloning and expression analysis of transcription factor gene DoWRKY1 in Dendrobium officinale.
Jun ZHAO ; Shi-wei SUN ; Can-can MENG ; Qing JIN ; Hong-hong FAN ; Yi LIN ; Yong-ping CAI
China Journal of Chinese Materia Medica 2015;40(14):2807-2813
WRKY transcription factors are novel transcriptional regulatory factors, which play an important role in regulating plant development, metabolism and other physiological processes. In this study, a new Dendrobium officinale WRKY transcription factor, designated as DoWRKY1 was cloned by using RT-PCR and RACE (GenBank Accession No. KF953910). Bioinformatic analysis demonstrated that, the full-length cDNA of DoWRKY1 was 1,704 bp. And DoWRKY1 contained a 1,629 bp open reading frame (ORF) that encoding a peptide of 542 amino acid residues. The putative DoWRKY1 protein contained two conserved WRKY domains and it belonged to the group I WRKY family protein. Yeast one-hybrid experiment showed that DoWRKY1 had transcriptional activation ability in yeast, and it could activate the expression of downstream report genes (His3 and Ade2). Semi-quantitative RT-PCR experiment showed that DoWRKY1 expressed in roots, stems, leaves and protocorm-like bodies. Real-time qRT-PCR proved that DoWRKY1 could be induced by methyl jasmonate (MeJA) and chitosan (Chitosan), and the expression level of this gene can reach the expression peak at 2 h and 1 h, respectively. These results are useful for further determination of the regulation function of this gene in secondary metabolism of D. officinale.
Cloning, Molecular
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Dendrobium
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genetics
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Gene Expression Regulation, Plant
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Plant Proteins
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genetics
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Transcription Factors
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genetics