Objective To explore the prevention and nursing strategies of contrast-induced nephropathy (CIN) in patients with congestive heart failure (CHF) within peri-operation period of cardiac resynchronization therapy (CRT). Methods Thirty inpatients with CHF from June 2007 to September 2009 were studied. All 30 patients received hydration therapy within peri-operation period and different contrast agents were administrated based on renal function of patients. The prevention and nursing measures, such as strictly limited the dosage of contrast agents were adopted. Results The heart function was significantly improved in all patients. There were no changes in renal function of 24 patients. However, 6 patients occurred the symptom of serum creatine increasing and 2 of whom was diagnosis as CIN and 1 patient degenerated into end stage renal function which relied on dialysis. Conclusions The comprehensive prevention and nursing measures including carefully evaluating renal function, taking the therapies of hydration and alkalization therapy can effectively prevent CIN for CHF patients receiving CRT.

Objective To explore appropriate health education method for patients receive coronary intervention operation to alleviate patients' anxiety and provide some scientific evidence for clinical to launch high quality nursing education.Methods According to the different days,totals of 420 patients with first coronary intervention operation were selected and randomly divided into control group (210 cases )and experimental group (210 cases).Control group received individual pattern health education,while experimental group received group patten health education.All patients were investigated with self-anxiety scale(SAS) before and after health education,then the incidence of anxiety and SAS score between two groups were compared.Results Before health education,64.8％ patients of control group and 65.7％ patients of experimental group existed anxiety,and the difference was statistically significant (x2=0.04,P ＞0.05 ).After education,the anxiety incidence of two groups lower than before education,experimental group ( 17.1％ ) was lower than control group( 27.6％ ),and the difference was statistically significant( x2=6.63,P ＜0.05 ).Before education,the SAS score of control group was ( 53.85 ± 3.78 ) and experimental group was ( 53.57 ± 4.89 ),and the difference was no statistically significant ( P ＞ 0.05 ).After education,the SAS score of control group and experimental group was (45.65 ±0.94),(42.18 ± 1.29),respectively,lower than before education,and the difference was statistically significant ( t=6.24,7.76,respectively; P ＜ 0.05 ).Also,the SAS score of experimental group lower than that of control group the difference was statistically significant ( t=7.39,P ＜0.05 ).Conclusions Health education for patients with coronary intervention can alleviate their pre-operation anxiety,and group patten health education is better than individual pattern health education in reducing anxiety patients number and alleviate patients' anxiety.

Objective To elevate student nurses' hand hygiene compliance through knowledge,attitude,belief,and practice(KAP) model.Methods 82 student nurses were selected and received KAP hand hygiene training.The knowledge,practical compliance and qualification of hand hygiene were observed before and after training.Results Hand hygiene and washing hand indication awareness rate were 92.68％ and 97.56％ after training,which were higher than those before training (46.34％ and 64.63％,respectively),and the difference was significant (x2 =36.03,25.04,respectively; P ＜ 0.01 ) ; Other awareness rates of hand hygiene after training were significantly improved than those before training ( P ＜ 0.01 ).The total compliance rate of hand hygiene of student nurses after training ( 82.97％ ) was significantly higher than that before training (50.92％) (x2 =316.08,P＜0.01 ).The qualified hand hygiene ratio was elevated from 58.54％ to 92.68 ％( x2 =25.92,P ＜ 0.01 ).Conclusions KAP model was effective towards student nurses' hand hygiene compliance and worthy for application.

Objective To investigate the effects of smad7 on transdifferentiation and collagenⅠsynthesis in advanced glyeosylation end-products(AGE)-stimulated NRK52E cells.Methods NRK52E cells were transferred by pTet-on plasmid system and the cell lines of doxycycline(Dox)-regulated Smad7 expression were selected for the study.Transnuclear location of p-Smad2/3 was examined with immunocytochemistry.The mRNA and protein expressions of Smad7,?-SMA,E-cadherin,collagenⅠwere detected with RT-PCR and Western blot. Results AGE-induced expressions of Smad7 mRNA and protein were further increased in NRK52E cells by the addition of Dox in a dose-dependent manner.Overexpression of Smad7 caused a marked inhibition of p-Smad2/3 transnuclear location at 30 min(68.3% vs 31.2%,P

Administration, Oral ; Adult ; Alanine Transaminase ; blood ; Antiviral Agents ; administration & dosage ; therapeutic use ; DNA, Viral ; blood ; Female ; Guanine ; administration & dosage ; analogs & derivatives ; pharmacology ; therapeutic use ; Hepatitis B e Antigens ; blood ; Hepatitis B virus ; drug effects ; Hepatitis B, Chronic ; drug therapy ; immunology ; Humans ; Lamivudine ; administration & dosage ; pharmacology ; therapeutic use ; Male ; Treatment Outcome ; Young Adult

OBJECTIVEThe goals of this work was to analyse the cost of Shenqi Fuzheng injection-an extraction of a Chinese traditional herbs on reducing adverse effects in lung cancer patients during chemotherapy.

METHODSIn a randomized cross-over trial, each patient completed two identical cisplatin-based chemotherapy cycles, one with Shenqi Fuzheng injection, another without Shenqi Fuzheng injection. Adverse effects and change scores of quality of life (QOL) during chemotherapy were compared in tow cycles. The direct cost dealing with adverse effect and cost-effectiveness analysis were taken.

RESULTSOne hundred and thirty were enrolled with 123 of whom were evaluable. The patient characteristics were well balanced between the two groups. The chemotherapy cycles with Shenqi Fuzheng injection spent 220.5 more Chinese yuan, but the adverse effect of leukopenia, thrombocytopenia and vomiting were slight different and the change of score of several QOL domains showed significant better as compared to those in another cycle.

CONCLUSIONShenqi Fuzheng injection could reduce the severity of toxicity related to chemotherapy and improve the QOL of patients and had some benefits in terms of cost-effectiveness.

Aged ; Antineoplastic Agents ; adverse effects ; Cost-Benefit Analysis ; Costs and Cost Analysis ; Cross-Over Studies ; Drugs, Chinese Herbal ; economics ; therapeutic use ; Female ; Humans ; Injections ; Lung Neoplasms ; drug therapy ; Male ; Middle Aged

Objective To explore the characteristics of human plague using a sero-epidemiologic method in the source of the three rivers area in Qinghai for possible plague control strategies. Methods Investigate human plague sero-epidemiologically in the source of 4 counties in the three rivers area in Qinghai. The human serum would be tested to confirm the sew-positive rate for plague F1 antibody using indirect hemagglutination assay(IHA). Results A total of 2508 local participants were tested in 4 counties, the overall plague sero-positive rate was 2.31%(58/2508). This represents a statistically significant difference with 4 counties(X2=19.30,P<0.01). The sew-positive rate for males and females were 2.54% (32/1261) and 2.09% (26/1247), respectively. There were no statistically significant differences between males and females(X2= 0.65,P 0.05). The sero-positive rate in herdsman, cadre, Tibetan, Hart nationalities were 3.54% (44/1243), 6.47% (11 / 170), 2.40% (56/2335) and 1.47% (2/136), respectively. The sero- positive rate increased with age. The highest titre for human plague serum antibody was 1 : 640. Conclusion There were occult infections of plague in the population on source of three rivers area in Qinghai. Sero-epidemiologic data revealed that the human plague sero-positive rate was closely correlated with the local animal plague.

Adult ; Blood Patch, Epidural ; Female ; Humans ; Intracranial Hypotension ; diagnosis ; therapy ; Magnetic Resonance Imaging ; Myelography ; Tomography, X-Ray Computed

OBJECTIVESTo detect the loss of heterozygosity (LOH) of circulating DNA in the plasma of patients with hepatocellular carcinoma (HCC), and to assess its potential as a clinical predictive marker.

METHODSThree high-polymorphic microsatellite markers D8S277, D8S298 and D8S1771 located at chromosome 8p were selected to detect LOH in plasma DNA of 62 HCC patients. The associations between LOH and its clinicopathological features, including HBsAg, liver cirrhosis, serum AFP level, tumor size, tumor cell differentiation, and intrahepatic metastasis were also examined.

RESULTSIn plasma DNA of the 62 HCC patients, LOH was found at one or several loci in 36 (58.1%), and heterozygosity at D8S277, D8S298, and D8S1771 loci was 74.2% (46/62), 75.8% (47/62), and 69.4% (43/62), respectively. LOH frequency at D8S277, D8S298 and D8S1771 was 32.6% (15/46), 44.7% (21/47), and 46.5% (20/43), respectively. LOH in plasma DNA was more frequently detected in the patients with intrahepatic cancer metastasis than those without metastasis (62.5 percent vs. 26.1 percent, P < 0.05); however, no statistically significant correlations were observed between LOH at these loci and other clinicopathological features analyzed in this study.

CONCLUSIONSLOH at D8S298 in plasma DNA may be a potential predictive marker of intrahepatic metastatic recurrence after surgical resection of the HCC.

Adult ; Aged ; Aged, 80 and over ; Carcinoma, Hepatocellular ; blood ; genetics ; Chromosomes, Human, Pair 8 ; DNA ; blood ; Female ; Humans ; Liver Neoplasms ; genetics ; Loss of Heterozygosity ; Male ; Middle Aged

BACKGROUNDRenal biopsy is necessary for diagnosing the pathological changes of primary nephrotic syndrome (NS). However, it is invasive, time-consuming and can not be performed frequent on the same patient. Thus, development of a non-invasive and rapid diagnostic method may improve clinical patient management.

METHODSProteomic tool magnetic bead-based matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MB-based MALDI TOF MS) was applied to serum to determine peptidome patterns that are characteristic of different pathological changes.

RESULTSSerum specimen from 114 patients with NS (62 were minimal change disease (MCD), 30 were membranous nephropathy (MN), and 22 were focal segmental glomerulosclerosis (FSGS)) and 60 normal individuals were analyzed using MB-based MALDI TOF MS. The peptidome pattern was generated by genetic algorithms using a training set of 31 MCD, 15 MN, 11 FSGS and 30 normal individuals and was validated by an independent testing set of the remaining samples. The serum peptidome pattern, based on a panel of 14 peaks, accurately recognized samples from MCD, MN, FSGS and healthy control with sensitivities of 93.5%, 86.7%, 63.6% and 90.0%, and specificities of 98.2%, 94.4%, 100% and 89.5%, respectively. Moreover, one peptide from peptidome pattern was identified by liquid chromatography tandem mass spectrometry (LC MS/MS) as fibrinogen A.

CONCLUSIONDetection of the serum peptidome pattern is a rapid, non-invasive, high-throughout, and reproducible method for identifying the pathological patterns of patients with nephrotic syndrome.

Adult ; Female ; Humans ; Male ; Middle Aged ; Nephrotic Syndrome ; blood ; Peptides ; blood ; Proteomics ; methods ; Reproducibility of Results ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; methods ; Young Adult