ObjectiveTo observe the effect of integrated traditional Chinese medicine therapy on pelvic inflammation. Methods40 patients of chronic pelvic inflammation were as the group of integrated traditional Chinese medicine therapy and received Chinese herbal medicine treatment (by taken orally, clysis and hot compress), acupuncture, mental state therapy etc. Other 36 cases were as the control group and only received Chinese herbal medicine treatment by taken orally. Then effects of two groups were compared. ResultsIt shows that the group of integrated traditional Chinese medicine therapy got obviously better results than the control group (P<0.01). ConclusionsThe integrated traditional Chinese medicine therapy is more effective on chronic pelvic inflammation.

Acute Disease ; Adult ; Angioplasty, Balloon, Coronary ; adverse effects ; Humans ; Hypopituitarism ; etiology ; therapy ; Male

Chronic Disease ; Constipation ; surgery ; Humans

Though there are different kinds of view on diabetic nutritional therapy for a long time,it is the basic treatment method on diabetes mellitus(DM).With some result of clinical research on diabetic nutritional therapy,people knew it more and more.To discuss new diabetic nutritional therapy is of great benefit to improve the curative effect and prolong the syndrome of DM.

Background Excimer laser corneal refractive surgery is widely used because it reduces the dependency of refractive error eye to glasses.However,the adverse effects of anti-inflammatory drugs is a problem after operation.Lotemax eyedrops is a kind of steroidal anti-inflammatory drug,with a good effect on the operative eye.But its influence on intraocular pressure(IOP) after long-term topical administration is less studied.Objective This study was to investigate the influence of lotemax eyedrops on IOP after long-term topical administration in myopic eyes received excimer laser corneal refractive surgery.Methods An one-month retrospective case serial study was adopted.The case history data of 1552 eyes from 1552 Patients who received excimer laser corneal refractive surgery were collected in Tangshan Eye Hospital from 2011 January to 2012 January,including 321 eyes with laser in situ keratomileusis(LASIK),608 eyes with sub-Bowman keratomileusis (SBK) and 623 eyes with femtosecond laser for flap excimer laser in situ keratomileusis(FS-LASIK).Lotemax eyedrops was topically administered after operation 4 times per day initial and then decreased 1 time weekly for 1 month.IOP was measured in before,1 week,2 weeks,3 weeks,4 weeks after operation with non-contact tonometer.The increasing range of IOP was divided into ＜5 mmHg,5-15 mmHg and ＞ 15 mmHg after operation.Optical head parameters were measured by Heidelberg retina tomography when IOP increasing after operation.Results Within 1 month,elevated IOP was found in 47 of 1552 eyes after administration of lotemax eyedrops,including 3.12％ (10/321) in the LASIK group,3.29％ (20/608) in the SBK group and 2.73％ (17/623) in the FS-LASIK group,without significant difference among them(x2 =1.95,P＞0.05).The IOP elevated 1 week after operation and remained normal after that till drug withdrawal in all eyes.There were no significant differences in the disk area (1.65 ±0.44) mm2 vs.(1.66 ±0.44) mm2,disk volume (0.40 ±0.09)mm3 vs.(0.39±0.08) mm3 and mean nerve fiber layer thickness (0.28 ± 0.08) mm vs.(0.29 ± 0.07) mm in IOP elevation duration compared with 1 month after IOP recovery(t =0.34,0.81,0.48,P＞0.05).Conclusions Topical administration of 0.5％ lotemax eyedrops after excimer laser corneal refractive surgery leads to reversible high IOP.It is very important to monitor the IOP during use of the drug.

The internal audit is important for the management system of laboratory .The article stated how to effectively implement internal audit in drug testing laboratories in terms of the planning , checklist, pattern, problem observation , CAPA of non-compliance and application of results in internal audit .

Objective To establish the rapid purification of Coxsackievirus A16 using ultracentrifugation .And To prepare and i‐dentify the neutralizing monoclonal antibody against CA16 .Methods The CA16 culture supernatant was harvested and then con‐centrated by 100K capsule .The concentration of CA16 was purified by cesium chloride ultracentrifugation .Purification of CA16 were identified by transmission electron microscopy .BALB/c mice were immunized with inactivated CA16 .Spleen cells were harves‐ted and fused with SP2/0 myeloma cells ,hybridoma cell strain secreting mAb against CA16 were objected to screening .Character‐ization of the prepared mAb were analyzed by ELISA and microneutralization assay .Results The purified CA16 method of cesium chloride gradient ultracentrifugation was established ,TEM analysis was showed that CA16 particles have icosahedral structure ,the diameters of the viral particles were approximately 20-30 nm .Two hybridoma cell strains secreting mAb against CA16 were ob‐tained ,the subtypes of two mAbs were IgG2a ,the binding titers of Anti/CA16/5 and Anti/CA16/10 were 103 and 104 respectively . Neutralizing titer of the two mAbs were 1∶256 and 1∶1 024 respectively .Conclusion Establishment method of cesium chloride gradient ultracentrifugation was performed to purify CA16 ,the two mAbs with neutralizing ability to against CA16 may become ap‐plication of treatment and vaccine .

Objective To observe the analgesic and sedative effects of oxycodone combined with dexmedetomidine and its effects on respiratory and cardiovascular functions during endotracheal intubation guided by fiberoptic bronchoscope at conscious state.Methods Forty patients with endotra-cheal intubation difficulty were randomly divided into sulfentanil group and oxycodone group.An initial induction dose of 1 μg/kg dexmedetomidine was given followed by an maintaining infusion of 0.2 μg·kg-1 ·h-1 in sulfentanil group,additionally,0.2 μg/kg sulfentanil was given intravenously. Patients in oxycodone group received intravenous infusion of oxycodone 0.08 mg/kg instead.The changes of HR,MAP and SpO 2 were monitored during medication and intubation.The Ramsay scores before intubation, the PET CO 2 and comfort degree after intubation were recorded. Results There were no statistically significant differences in hemodynamic changes,Ramsay scores and comfort level between the two groups during drug-injection and intubation.SpO 2 of patients in oxycodone group was higher than that of sulfentanil group after injection of drugs,but PET CO 2 of oxycodone group after endotracheal intubation was lower.Conclusion The combination of oxycodone and dexmedetomidine can be safely used in awake tracheal intubation,for it shows effective sedation and analgesia,less respiratory depression and other side-effects compared with sufentanil.

Objective To explore the etiologies and characteristics of esophageal motility in patients with non-obstructive dysphagia by esophageal high-resolution manometry (HRM).Methods From November 2011 to August 2015,233 patients with non-obstructive dysphagia diagnosed by HRM were retrospectively analyzed.All the patients received gastroendoscopy to exclude obstructive dysphagia.Results Among 233 patients with non-obstructive dysphagia,there were 160 cases of achalasia,38 cases of nonspecific esophageal motor disorder (13 cases of low amplitude peristalsis or absent peristalsis,seven cases of synchronous contraction or rapid contraction,three cases of distal esophageal spasm,six cases of increased resting upper esophageal sphincter pressure (UESP),three cases of reduced UESP,six cases of lower esophageal sphincter (LES) incomplete relaxation),five cases of gastroesophageal reflux disease,four cases of scleroderma,two cases of Jackhammer esophagus,and 24 cases with normal esophageal motility.Conclusions Achalasia is the most common cause of non-obstructive dysphagia,followed by nonspecific esophageal motor disorder.Esophageal HRM is an important method for the diagnosis of nonobstructive dysphagia,especially for unexplained dysphagia.