2.The collecting and processing system of the sound signal of larynx.
Qing JIAO ; Yong-xin GUO ; Qing-guo MENG ; Xian-yun WANG
Chinese Journal of Medical Instrumentation 2002;26(3):174-176
This paper presents a new kind of collecting and processing system of the sound signal of larynx where double sound cards and software filter are used. The installation of the double sound cards and processing proposal of software are mainly discussed and a new kind of ADC method of dual-channel sound signal is put forward in this paper. This system has the feature of reliable performance, simple installation and easy maintenance.
Algorithms
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Equipment Design
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Humans
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Larynx
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physiology
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Microcomputers
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Signal Processing, Computer-Assisted
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instrumentation
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Software
;
Sound
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Speech Production Measurement
;
instrumentation
4.Clinical analysis on 175 cases of occupational brucellosis.
Yi-wen JIANG ; Qing WANG ; Ruo-xin ZHAO ; Shu-ke GE ; Xin-wei GUO
Chinese Journal of Industrial Hygiene and Occupational Diseases 2013;31(11):861-863
Adult
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Aged
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Brucellosis
;
diagnosis
;
therapy
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Female
;
Humans
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Male
;
Middle Aged
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Occupational Diseases
;
diagnosis
;
microbiology
;
therapy
5.Study on the B cell linear epitopes of rabies virus CVS-11 nucleoprotein.
Xin-Jun LV ; Xin-Xin SHEN ; Peng-Cheng YU ; Hao LI ; Li-Hua WANG ; Qing TANG ; Guo-Dong LIANG
Chinese Journal of Virology 2014;30(3):253-256
To study the B cell linear epitopes of rabies virus CVS-11 nucleoprotein, peptides were synthesized according to the amino acid sequences of B cell linear epitopes. Linear epitopes predicted by bioinformatics analysis were evaluated with immunological techniques. Indirect enzyme-linked immunosorbent assay showed that titers of antibodies to peptides (355-369 and 385-400 residues of rabies virus CVS-11 nucleoprotein) were above 1:12 800 in mouse sera. The antibodies recognized denatured rabies virus CVS-11 nucleoprotein in Western blot analysis. Purified anti-peptide antibodies recognized natural rabies virus CVS-11 nucleoprotein in BHK-21 cells in indirect fluorescent antibody test. The 355-369 and 385-400 residues of rabies virus CVS-11 nucleoprotein were validated as B cell linear epitopes.
Amino Acid Sequence
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Animals
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Antibodies, Viral
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immunology
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Epitope Mapping
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Epitopes, B-Lymphocyte
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chemistry
;
genetics
;
immunology
;
Female
;
Humans
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Male
;
Mice
;
Mice, Inbred BALB C
;
Molecular Sequence Data
;
Nucleoproteins
;
chemistry
;
genetics
;
immunology
;
Rabies
;
immunology
;
virology
;
Rabies virus
;
chemistry
;
genetics
;
immunology
6.Effect of terpene penetration enhancer and its mechanisms on membrane fluidity and potential of HaCaT keratinocytes.
Yi LAN ; Jing-yan WANG ; Yan LIU ; Qing-guo RU ; Yi-fei WANG ; Jing-xin YU ; Qing WU
China Journal of Chinese Materia Medica 2015;40(4):643-648
The aim of this paper was to investigate the effect of terpene penetration enhancers on membrane fluidity and membrane potential using HaCaT keratinocytes, and study the potential mechanisms of these terpene compounds using as natural transdermal penetration enhancer. Six terpene compounds, namely menthol, limonene, 1,8-cineole, menthone, terpinen-4-ol and pulegone, were chosen in this study on account of their good penetration-enhancement activities. The cytotoxicity of these terpene compounds was measured using an MTT assay. The fluorescence recovery after photobleaching (FRAP) technique was employed to measure the change of membrane fluidity of HaCaT cells. The flow cytometer was used to study the alteration of membrane fluidity of HaCaT cells, and investigate the effect of terpene compounds on intracellular Ca2+. It was found that 6 terpene compounds possessed low cytotoxicity in comparison to the well-established and standard penetration enhancer azone. Those terpene compounds could significantly enhance HaCaT cells membrane fluidity and decrease HaCaT cells membrane potentials. Meanwhile, after treated with various terpene compounds, the Ca2(+)-ATPase activity and intracellular Ca2+ of HaCaT cells was decreased significantly. Terpene penetration enhancers perhaps changed the membrane fluidity and potentials of HaCaT cells by altering the Ca2+ balance of the cell inside and outside, resulting in the low skin permeability to increase the drug transdermal absorption.
Cell Line
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Drugs, Chinese Herbal
;
pharmacokinetics
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Humans
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Keratinocytes
;
drug effects
;
metabolism
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Membrane Fluidity
;
drug effects
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Skin Absorption
;
drug effects
;
Terpenes
;
pharmacokinetics
7.Study on chemical constituents of Achillea alpina.
Xiao-qing CHEN ; Meng WANG ; Xin ZHANG ; Wei-wei GUO ; Xia WU
China Journal of Chinese Materia Medica 2015;40(7):1330-1333
Twelve compounds were isolated from the aerial parts of Achillea alpina by column chromatographies on silica gel, Sephadex LH-20, and semi-preparative HPLC. The structures were elucidated on the basis of spectral analysis. The compounds were identified as pellitorine(1), 8,9-dehydropellitorine(2), (E,E)-2,4-undecadien-8, 10-diynoic acid isobutylamide(3), (E,E)-2,4-tetradecadien-8,10-diynoic acid isobutylamide(4),sintenin(5), 4',5,7,8-tetramethoxyflavone(6), chrysoplenetin(7), formononetin(8), aurantiamide(9), asperglaucide(10), artemetin(11), and eupatorin(12). compounds 1-5 were isolated from this plant for the first time, and compounds 6-10 were isolated from the genus Achillea for the first time.
Achillea
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chemistry
;
Chromatography, High Pressure Liquid
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Drugs, Chinese Herbal
;
chemistry
;
isolation & purification
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Molecular Structure
;
Spectrometry, Mass, Electrospray Ionization
8.Proximal epithelioid sarcoma: a case report.
Qing-zhu WEI ; Yan-hui LIU ; Heng-guo ZHUANG ; Dong-lan LUO ; Xin-lan LUO
Chinese Journal of Pathology 2006;35(10):638-639
Antigens, CD34
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metabolism
;
Cytokines
;
metabolism
;
Diagnosis, Differential
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Humans
;
Immunohistochemistry
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Male
;
Middle Aged
;
Mucin-1
;
metabolism
;
Perineum
;
pathology
;
Phosphopyruvate Hydratase
;
metabolism
;
Sarcoma
;
metabolism
;
pathology
;
surgery
;
Vimentin
;
metabolism
9.Evolutionary relationships of G3 GARV isolated from pigs and humans in Lulong County, Hebei Province, China.
Yan-Qing GUO ; Jing-Yao XIANG ; Xin MA ; Dan-Di LI ; Zhao-Jun DUAN
Chinese Journal of Virology 2014;30(4):417-422
This study aimed to amplify major genome segments (VP7, VP4, VP6, VP2 and NSP2-5) of porcine G3 group A rotavirus (GARV) LLZ212 isolated in our laboratory, determine their genotypes, and explore the evolutionary relationships between G3 GARV strains isolated from humans and pigs in Lulong County, Hebei Province, China. Major genome segments of seven GARV strains were amplified by reverse transcription-polymerase chain reaction (RT-PCR) and the segments were sequenced. The genome segments of seven GARV strains were determined by the online RotaC genotyping tool (RotaC v2.0). The reference sequences of each GARV genome segment were downloaded from GenBank. Homology and phylogenetic evolutionary analyses were conducted using the MEGA 5.0 and DNAStar software packages. LLZ212 isolated from pigs in Lulong had the following genotype: G3-P[8]-I5-C1-N1-T1-E1-H1. All human GARV strains had the following genotype: G3-P[8]-I1-C1-N1-T1-E1-H1. The VP7, VP4, NSP4 and NSP5 genes of the LLZ212 strain had the highest nucleotide identities with the human GARV E885, CMH014/07, Wa and RMC321 strains, respectively, and these clustered together in a sublineage. The VP6, NSP4 and NSP5 genes of the LLZ212 strain shared the highest nucleotide identities with the porcine GARV PRG921 strain, while VP2 associated most closely with porcine GARV OSU strain, and these also clustered in a sublineage. A rare porcine G3-P[8]-I5-C1-N1-T1-E1-H1 GARV strain was identified, which may represent a reassortment between porcine and human viruses. In conclusion, the VP7, VP4, NSP4 and NSP5 genes of LLZ212 share high levels of sequence identity with human GARV, while VP2, VP6, NSP2 and NSP3 cluster with porcine GARV.
Animals
;
Capsid Proteins
;
genetics
;
Child, Preschool
;
China
;
epidemiology
;
Evolution, Molecular
;
Genotype
;
Humans
;
Infant
;
Male
;
Molecular Sequence Data
;
Phylogeny
;
Rotavirus
;
classification
;
genetics
;
isolation & purification
;
Rotavirus Infections
;
epidemiology
;
veterinary
;
virology
;
Swine
;
Swine Diseases
;
epidemiology
;
virology
;
Viral Nonstructural Proteins
;
genetics