1.Case of up-rushing gas syndrome and chest apoplexy.
Hong-Wei JI ; Qing-Zhang FENG ; Fang-Ming LIU
Chinese Acupuncture & Moxibustion 2013;33(10):904-904
Acupuncture Therapy
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Chest Pain
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therapy
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Female
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Humans
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Middle Aged
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Stroke
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therapy
2.ICP-MS method for the determination of uranium concentration and 235U/238U ratio in urine and its uncertainty evaluation
Liangliang YIN ; Qing TIAN ; Wei HUANG ; Xianzhang SHAO ; Yanqin JI
Chinese Journal of Radiological Medicine and Protection 2017;37(5):376-380
Objective To establish a method of analyzing total uranium and 235U/238U ratio in urine using inductively coupled plasma mass spectrometry (ICP-MS) and uncertainty assessment.Methods Urine sample was digested with HNO3 and H2O2,and total uranium was determined using ICP-MS method directly.The digested urine sample was separated to concentrate uranium with tributyl phosphate (TBP) column,and 235U/238U ratio was analyzed using ICP-MS.The uncertainty was evaluated through sample pre-treatment,measurement and standard curve calculation.Results The recovery of total uranium in urine was 98.4%-102.4%,detection limit was 0.002 μg/L.The relative expanded uncertainty of total uranium concentration in urine was 0.26 (k =2).235 U/238 U ratio was 0.001 1 (k =2).Conclusions This study offers a low detection limit,good recovery and precision method for rapid determination of total uranium and 235U/238 U ratio in urine samples.It is potential used for both in occupational exposure assessment and nuclear emergency situation.The uncertainty evaluation of total uranium and 235U/238U ratio in urine are reliable.
3.Comparison of the clinical manifestation and lung function between RSV and hMPV lower respiratory tract infection.
Wei JI ; Zheng-rong CHEN ; Yu-qing WANG
Chinese Journal of Pediatrics 2009;47(1):71-73
Child, Preschool
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Humans
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Infant
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Infant, Newborn
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Lung
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physiopathology
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virology
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Male
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Metapneumovirus
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isolation & purification
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Paramyxoviridae Infections
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diagnosis
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physiopathology
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Respiratory Function Tests
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Respiratory Syncytial Virus Infections
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diagnosis
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physiopathology
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Respiratory Syncytial Viruses
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isolation & purification
4.Investigation of expectations of building clinical practice program for nursing postgraduates
Yaru Ren ; Xiuhong Wei ; En'en Jia ; Qing Ji
Chinese Journal of Practical Nursing 2015;31(1):23-25
Objective We tried to explore nursing postgraduates' expectations of clinical practice,and build a set of practicable clinical practice program which contributed to cultivate comprehensive ability of nursing postgraduate.Methods Using judgment sampling and snowball sampling,the questionnaire survey was conducted among 111 nursing postgraduates,and expectations of clinical practice of postgraduates with dif-ferent ages and different work experiences were compared.Results Most nursing postgraduates hold that the clinical practice was necessary.Nursing postgraduates with different ages and work experiences had different expectations of chnical practice,and the differences were statistically significant,x2 value was 11.594 and 4.416,P < 0.05.Nursing postgraduate with different working experience showed statistical difference in clinical expectation of total practice time,x2=1 1.290,P < 0.05.Conclusions Nursing post-graduates with different ages and work experiences have different expectations of clinical practice.Colleges should build a set of reasonable clinical practice program,based on training objectives,research direction,practice expectations of nursing postgraduates.
5.Mongolian medicine cha gan beng ga regulated activity of biomarker PGC-1α.
Han-Qing LI ; Jia-Yin XU ; Lu YU ; Ji-Le XIN ; Ji-Wen WEI
China Journal of Chinese Materia Medica 2014;39(17):3371-3375
OBJECTIVETo investigate the regulation of Cha Gan Beng Ga on the activity of biomarker PGC-1α in vivo and in vitro, and lay the foundation for studying the efficacy result of Cha Gan Beng Ga on xenograft tumor model and extracting active constituents.
METHOD(1) The coarse powder of Cha Gan Beng Ga was extracted with 70% ethanol solution through heating and refluxing, and finally was used to freeze dry powder. (2) 50 mg x kg(-1) of freeze-dried power was orally administrated to KM and C57BL/6J mice once daily, lasting for 5 consecutive days; different concentrations of extracted materials was given to non-small cell lung cells A549. (3) The expression level of PGC-1α mRNA was quantitatively determined in lung tissue of mice and non-small cell lung cells A549.
RESULTThe expression levels of PGC-1α in lung tissue of different mice strains had an increasing tendency. Furthermore, the expression levels of PGC-1α in non-small cell lung cells A549 also had an increasing tendency, showing dose and time-dependent relationships.
CONCLUSIONMongolian Medicine Cha Gan Beng Ga could induce the over-expression of PGC-1α mRNA in lung tissue of mice and in non-small cell lung cells A549. The present results will lay foundation for studying the efficacy result of antitumor and active constitutes in future.
Aconitum ; chemistry ; Animals ; Biomarkers, Tumor ; genetics ; Carcinoma, Non-Small-Cell Lung ; genetics ; pathology ; Cell Line, Tumor ; Dose-Response Relationship, Drug ; Gene Expression Regulation, Neoplastic ; drug effects ; Humans ; Lung ; drug effects ; metabolism ; Lung Neoplasms ; genetics ; pathology ; Male ; Medicine, Mongolian Traditional ; Mice, Inbred C57BL ; Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha ; Plant Extracts ; pharmacology ; Reverse Transcriptase Polymerase Chain Reaction ; Time Factors ; Transcription Factors ; genetics
6.Study on serum levels of vitamin D and calcium and expression of tissue calcium-sensing receptor in the breast cancers
Jun YE ; Wei ZHENG ; Peizhi FAN ; Qing XIE ; Chaojie ZHANG ; Yang LI ; Chunyi JI ; Wei ZHOU
Journal of Chinese Physician 2012;14(9):1157-1160
ObjectiveTo explore the relationship among 25-hydroxyvitamin D,serum calcium,calcium-sensing receptor,and breast cancer. Methods The expressions of calcium-sensing receptor (CaSR) in primary breast cancer,breast benign tumors,and normal breast tissue beside tumors were determined by immunohistochemistry S-P method as well as the concentration of serum 25 (OH) D and serum calcium in breast cancer and breast benign tumors by Enzyme-linked immunosorbent assay (ELISA),Tribromoarsenazo Ⅲ method.ResultsSerum 25 (OH) D level of breast cancer was significantly lower than the breast benign tumors [ (34.13 ± 14.14) nmol/L vs (50.29 ± 25.65 ) nmol/L,t =2.870,P =0.001 ].Serum level of 25 ( OH ) D in lymph node metastasis positive patient was lower than that in negative group [ (30.8 ± 9.71 ) nmol/L vs (43.7 ± 23.59) nmol/L,t =2.467,P =0.021 ].The positive expression of CaSR in breast cancer(88.9% )was higher than breast benign tumors(60%,x2 =6.717,P < 0.01 ) and normal breast tissue beside tumors (60%,x2 =5.628,P < 0.05 ).ConclusionsConcentration of serum 25 (OH)D and expression of calcium-sensing receptor in the tissues may be associated with occurrence,development and prognosis of breast cancer.
7.Effects of HBV infection on hepatic fibrosis and level of Th1/Th2 cytokines in the patients with Schistosomiasis japonica
Zhong-Wei JIA ; De-Yong CHU ; Wei WANG ; Qing-Li LUO ; Ji-Long SHEN ;
Chinese Journal of Laboratory Medicine 2003;0(10):-
Objective The levels of Thl cytokines(IL-10 and IL-13)and Th2 cytokines(INF-? and TNF-?)were determined in the sera of patients with Schistosomiasis japonica in order to find the relationship between cytokines and severe hepatic fibrosis(HF)in schistosomiasis.Methods A total of 358 patients with advanced Schistosomiasis japonica were examined by ultrasound.68 HBsAg negative patients were chosen randomly as experimental control.Among them,39 patients were found to have mild HF and 29 were severe HF.The sera levels of Thl and Th2 cytokines were determined with ELISA.Results Among these 358 patients,83(23.2%)were HBsAg positive.Neither earlier nor severer hepatic fibrosis was noted in the patients who had been simultaneously infected with HBV than those only infected with schistosomiasis. There was a significant difference between mild[ 1.60(1.30-12.14)ng/L]and severe[ 4.20(1.43- 52.07)ng/L]HF patients in the level of IL-10(Z=-3.907,P0.05)was found in level of IFN-?,between severe[3.12(1.38-66.14)ng/L]and mild[5.87(1.33-216.33)ng/ L]HF subjects.Our observation did not reveal any obvious difference of TNF-? between severe[ 2.48(0.79 -19.86)ng/L]and mild[ 2.28(0.67-15.72)ng/L]HF groups.Conclusions Patients infected with advanced shistosomiasis may become more susceptible to HBV.The results of the present investigation showed that a high level production of IL-13 was associated with severe HF.
8.Development of Tripterygium glycosides nano-carries based on "nanoemulsion-gels" and its pharmacodynamics.
Qing GU ; Ben-Ming YOU ; Di-Shun YANG ; Yong-Hua PAN ; Wei SHU ; Ji-Yong LIU
China Journal of Chinese Materia Medica 2015;40(1):73-78
The aim of this study is to develop the Tripterygium glycosides nanoemulsion gels and investigate its pharmacodynamics. Oleic acid was used as oil phase, polyoxyethylene castor oil as surfaetant, and 1,2-propanediol as cosurfactant to screen the formula of Tripterygium glycoside nanoemulsion using the pseudo-temary phase diagrams. Then the nanoemulsion gels was prepared. The ICR mouse ears were sensitazated by 7% DNCB, and then were excited by 0.3% DNCB to stimulate the model of mouse chronic dermatitis and eczema. The concentrations of IFN-γ, IL-4 and IL-8 in mouse blood were determined by ELISA. The results showed that Tripterygium glycosides nanoemulsion gels could significantly inhibit the swelling of mouse ears(P < 0.01) and ameliorate the edama and erythema of model mouse ears skin. Also it could significantly decrease the expression of IFN-γ and IL-4 in model mouse blood. Tripterygium glycosides nanoemulsion gels had a good therapeutic effect on mouse model of dermatitis and eczema. It was expected to provide a new and long-acting exterernal preparation for the treatment of dermatitis and eczema.
Animals
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Chemistry, Pharmaceutical
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instrumentation
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methods
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Dermatitis
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drug therapy
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immunology
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Drug Carriers
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chemistry
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Drugs, Chinese Herbal
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chemistry
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pharmacokinetics
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Emulsions
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chemistry
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Female
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Glycosides
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chemistry
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pharmacokinetics
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Humans
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Interleukin-4
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immunology
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Interleukin-8
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immunology
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Mice
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Mice, Inbred ICR
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Nanoparticles
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chemistry
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Tripterygium
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chemistry
9.“Happy Reading Club” established by undergraduates and its problems
Qing HOU ; Xiaoyan Lü ; Jiongjiong HUANG ; Wei JI ; Sheng YU ; Siyu ZHANG
Chinese Journal of Medical Library and Information Science 2014;(10):44-46
Establishment of the “Happy Reading Club” was to create a personal reading space for undergraduates. The reading situation of undergraduates was analyzed with the creation processes of “Happy Reading Club” summa-rized.The reading popularizing activities were described, including questionnaire investigation of reading, sign of DIY “tree”, and recommendation of classic booklist, with the existing problems pointed out.
10.The influence of LPS on the protein expression of related molecules in Smads and ERK1/2 signal pathway in LTC-14 cells
Qing ZHANG ; Kai CHEN ; Meili LU ; Wei XU ; Xiaohui XIANG ; Shihai XIA ; Runli JI
Chinese Journal of Pancreatology 2017;17(2):93-98
Objective To explore the influence of LPS treatment on related molecules in Smads and ERK1/2 signal pathway in pancreatic stellate cell line LTC-14.Methods LTC-14 cells were cultured in vitro, and were treated with LPS at different dose in different time points.Protein expressions of related molecules in Smads pathway and ERK1/2 pathway and α-SMA in LTC-14 Cells were examined by Western blot.Results On Treated LTC-14 cells by 0, 1, 5, 10, 20 and 50 mg/L LPS,protein expressions of Smad3 were 0.15±0.02, 0.37±0.02, 0.44±0.01, 0.46±0.02, 0.372±0.01 and 0.24±0.03;expressions of Smad7 were 0.79±0.05, 0.84±0.02, 0.55±0.03, 0.45±0.03, 0.34±0.02 and 0.92±0.07;p-ERK1/2 levels were 0.48±0.05, 0.74±0.03, 0.72±0.04, 0.89±0.02, 0.81±0.02 and 0.72±0.03;p-cPLA2 levels were 0.15±0.03, 0.30±0.01, 0.31±0.01, 0.30±0.02, 0.28±0.03 and 0.32±0.02;α-SMA levels were 0.56±0.06, 0.62±0.06, 0.54±0.04, 1.03±0.11, 1.39±0.08 and 1.28±0.10.The changes of protein expressions before and after LPS treatment were obvious (all P<0.01).The protein expressions of ERK1/2 were 0.56±0.03, 0.57±0.02, 0.53±0.02, 0.58±0.02, 0.59±0.05 and 0.55±0.04, which did not change obviously along with increased LPS dosages.LTC-14 cells treated with 10 mg/L LPS for 0, 1, 3, 6 and 9 h,the expressions of Smad3 were 0.69±0.05, 0.68±0.07, 1.02±0.14, 1.82±0.0 and 2.04±0.11,those of Smad7 were 2.77±0.10, 1.37±0.08, 1.45±0.14, 0.78±0.09 and 0.63±0.06,those of p-ERK1/2 were 0.16±0.03, 0.32±0.05, 0.79±0.03, 1.50±0.07 and 1.77±0.04,those of p-cPLA2 were 0.15±0.04, 0.32±0.06, 0.63±0.04, 0.95±0.04 and 1.49±0.10,those of α-SMA were 0.84±0.03, 1.26±0.21, 1.81±0.19, 4.28±0.26 and 4.37±0.15, all of which changed obviously as the treatment time increased (P<0.05 or 0.01).The expressions of ERK1/2 were 0.75±0.03, 0.72±0.02, 0.80±0.04, 0.74±0.03 and 0.85±0.09, which did not change obviously as the treatment time increased.Conclusions LPS could upregulate the expression of α-SMA in a time-and dose-dependent way, and activate intracellular Smads and ERK1/2 inflammatory pathways, which may be the potential molecular mechanism of the development of chronic pancreatitis.