Objective To explore the effect of Angiotensin Ⅱreceptor inhibitor on proliferation and apoptosis and cell cycle of human en-dometrial cancer cell line induced by estrogen.Methods Human endometrial cancer cells,ishikawa were cultured,and then treated with different concentrations of estrogen or saralasin.Cell proliferation was examined using MTT,and cell cycle and apoptosis were determined us-ing flow cytometry.Results Saralsin inhibited the proliferation of ishikawa cells.After treated with saralasin at 10 min after estrogen induc-tion,cycle was arrested at G1/G0 phase and S phase was reduced in ishikawa cell with significant difference between treatment group and control group.An increase in early apoptosis and late apoptosis occurred at 10min after treatment.Conclusion Saralsin inhibits endometrial cancer cell ishikawa proliferation,induces apoptosis and accumulation in G1/G0 phase in vitro.Therefore,saralsin may be helpful for the treat-ment of endometrial cancer.

The clinical supervision of counseling and psychotherapy have a closely connection with clinical employment.Leading the way in clinical supervision,many clinical supervision models has been extrapolated by theories of various psychotherapy,relating to psychoanalysis theory,person-centered approach theory,cognitive and behavior psychotherapy theory,system theory,structuralism theory,and so on.Recently,especial,different clinical supervision models have to expand beyond psychotherapy,such as developmental model of supervision and society role model of supervision.The study has summarized clinical supervision models of the field,in order to offer literature index and concept frame.

Castration-resistant prostate cancer is a fatal disease with rapid progress. This malignancy usually presents with metastasis and poor prognosis. This type of disease also often causes 100%mortality, of which the median survival time is less than 20 months. Thus, treatment of castration-resistant prostate cancer remains challenging, and the underlying mechanisms of this cancer have yet to be identified. Several new therapies for castration-resistant prostate cancer have been proposed, such as androgen receptor antago-nists, immunotherapeutic drugs, taxanes, antiangiogenic agents, radionuclides, and bone-targeting drugs. These therapies can im-prove the survival time of patients. The advances in the treatment of castration-resistant prostate cancer are briefly reviewed in this ar-ticle.

Objective To apply laparoscopy-assisted small incision surgery in complicated urological operation, and to summarize our experience. Methods We retrospectively analyzed the clinical data of 9 patients with renal tumors (3 with solitary kidney tumors, 2 with unilateral multiple tumors, 1 with bilateral tumors, and 3 with contralateral renal insufficiency), 3 patients with renal pelvic ureteropelvic junction obstruction (UPJO) and multiple stones, and 1 patient with distal ureteral tumor. All patients underwent laparoscopy-assisted small incision surgery in our department from Aug. 2007 to Jun. 2011. The key steps of the surgery were discussed and the clinical experience was summarized. Results (1) For 9 patients with renal tumors, the incision was 4-6 cm in length, the cold ischemia timewas (15 ±4) min, and the operation time ranged from 90 to 180 min. Drainage tubes were removed within 48 h after operation. No patient had bleeding, urinary fistula or other serious complications. (2) For the 3 patients with UPJO, the incision was 4-5 cm in length and the operation time was 110 to 190 min. Drainage tubes were removed within 3-5 d after operation. No patient had bleeding, urinary fistula or other serious complications. No patient had fever or back pain after removal of double-J stents. Intravenous pyelography showed no hydronephrosis and the renal structure was normal. (3) For the patient with ureteral tumor, the drainage tube was removed 3 d after operation and there was no complication. No patients in the present study used analgesic pump or analgesic medications after surgery. Conclusion Laparoscopy-assisted small incision surgery can reduce the length of incision, minimize injury, and protect renal function. Besides, the method is safe and capable of handling complicated situations, especially suitable for patients who are difficult to receive total laparoscopic surgery.

To apply laparoscopy-asiisted small incision surgery in complicated urological operation, and to summarize our experience. Methods We retrospectively analyzed the clinical data of 9 patients with renal tumors (3 with solitary kidney tumors, 2 with unilateral multiple tumors, 1 with bilateral tumors, and 3 with contralateral renal insuffieiency), 3 patients with renal pelvic ureteropelvic junction obstruction (UPJO) and multiple stones, and 1 patient with distal ureteral tumor. All patients underwent laparoscopy-assisted small incision surgery in our department from Aug. 2007 to Jun. 2011. The key steps of the surgery were discussed and the clinical experience was summarized. Results (1) For 9 patients with renal tumors, the incision was 4-6 cm in length, the cold ischemia time was (15±4) min, and the operation time ranged from 90 to 180 min. Drainage tubes were removed within 48 h after operation. No patient had bleeding, urinary fistula or other serious complications. (2) For the 3 patients with UPJO, the incision was 4-5 cm in length and the operation time was 110 to 190 min. Drainage tubes were removed within 3-5 d after operation. No patient had bleeding, urinary fistula or other serious complications. No patient had fever or back pain after removal of doubie-J stents. Intravenous pyelography showed no hydronephrosis and the renal structure was normal. (3) For the patient with ureteral tumor, the drainage tube was removed 3 d after operation and there was no complication. No patients in the present study used analgesic pump or analgesic medications after surgery. Conclusion Laparoscopy-assisted small ineieion surgery can reduce the length of incision, minimize injury, and protect renal function. Besides, the method is safe and capable of handling complicated situations, especially suitable for patients who are difficult to receive total laparoscopic surgery.

Papers on mycorrhizas were searched from SCI (Science Citation Index) database. In order to understand the research progress of mycorrhizas in China, the paper on mycorrhizas from 1989 to 2007 was analyzed with method of bibliometrics. Mycorrhizal researches showed an increasing tendency during 1989 to 2007, while the increase was obviously accelerated after 2000. Only 5.22% of the articles were published in journals with impact factor above 5, indicates that the academic levels of researches need being enhanced. Currently the research on mycorrhizas was mainly focused on Arbuscular mycorrhiza (AM), especially on effects of mycorrhizal fungi on plant physiology, effects of mycorrhizal fungi on resistance of host plants, Mycorrhizal diversity community and ecological distribution, and phytoremediation with Mycorrhizal plants. The future research should emphasize in phytoremediation with Mycorrhizal plants and identification of fungal species with molecular biological technology.

[Objective]To observe the effect of treatment on post-stroke hiccup by acupuncture and medicine.[Methods]58 cases were divided into 2 groups randomly,30 cases were taken the acupuncture and medicine treatment, 28 cases were injected metoclopramide as control. [Results]The curative effect in therapy group is much better than that of control group(P<0.05).[Conclusion]The therapy of acupuncture and medicine is superior to pure medicine in the treatment of post-stroke hiccup.

The role of central catecholamine (CA) and angiotensin Ⅱ (AⅡ) in one-kidney Grollman hypertensive rats was investigated. Arterial blood pressure was measured by indirect tail-plethysmography, and the contents of norepinephrine(NE), epinephrine(E) and AⅡ in hypothalamus and medulla oblongata were determined by means of fluorimetry and immunoradioassay respectively. The results showed that during the maintenance period of one-kidney Grollman hypertension the brain contents of NE E and AⅡ were significantly higher than that of the age-matched sham-wrapped rats. Separate intracerebroventricular injections of 6-OHDA and captopril not only induced significant decline of arterial blood pressure but also induced reduction of NE, E and AⅡ in the brain areas. Besides, the arterial baroreflex sensitivity in one-kidney Grollman hypertensive rats was markedly decreased. It suggests that the increase of NE, E and AⅡ and the decrease of the arterial baroreflex sensitivity may play an important role in one-kidney Geallmon hyportension.

In order to investigate the role of endothelin (ET) in blood pressure re-gulation, the depressor effect of ET_A receptor antagonist -BQ_(123) was observed. After in-travenous administration of BQ_(123) (0.1, 1.0 and 2.0 mg/kg) into spontaneously hyperten-sive rat(SHR) and normotensive WKY rat, their blood pressure decreased in a dose-depen-dent manner, which continued 40-60 min. The blood pressure reducing action of BQ_(123)in SHR was more potent than that in WKY rat. Intravenous injection of BQ_(123) intoWistar rat inhibited pressor reponse induced by exogenous ET-1(2?10~(-9)mol/kg) in adose-dependence manner. ET-1(10~(-7)mol/L) stimulated proliferation of cultured smoothmuscle cells from SHR aorta, i.e., increase in ~3H-thymidine and ~3H-Leucine, however, BQ_(123)effectively antagonized the above action of ET-1. These results suggested that endogenousET to some extent could be involved in the regulation of normotension and also in thedevelopment of hypertension, and that ET antagonists would be useful for clinical preven-tion and therapy of hypertensive diseases.

Objective: To investigate the effects of neferine ( Nef ) on the learning and memory function and the expression of Notch1 and SYN in hippocampus in the rats with chronic cerebral ischemia .Methods:Male SD rats (250-300g) were randomly divid-ed into the sham operation group(Sham), the model group(Mod), nimodipine (Nim) positive control group and Nef treatment group with 5 ones in each .The chronic cerebral ischemia ( CCI ) model was established using bilateral common carotid artery ligation ( 2-VO).The rats were orally administered with NS(5 mg· kg-1),Nim(1 mg· kg -1) and Nef (20 mg· kg-1),respectively for 21 days after the first day of operation .The body weight was recorded .The number of hippocampal neurons in the rats was observed by Nissel staining.The learning and memory function was evaluated by Morris water maze test .Notch1 and SYN protein expressions in hippo-campus were detected by IHC staining and Western blot .Results:Compared with the Mod group , Nef group could reverse the reduc-tion of body weight and the number of hippocampal neurons in hippocampus CA 1 region induced by 2-VO, increase the route and the time of platform finding, prolong the escape latency and decrease the number of platform cross on the 21st day, and the effects were bet-ter than those of Nim (P<0.05).Moreover, the down-regulated SYN and Notch1 protein expressions in CCI group were both increase after the Nef treatment on the 21st day.Conclusion:Nef has a protective effect on the function of learning and memory in CCI rats in-duced by 2-VO,which may be related with the strengthened Notch 1 pathway in CA1 region of hippocampus and synaptic plasticity .