Objective To explore the effect of Angiotensin Ⅱreceptor inhibitor on proliferation and apoptosis and cell cycle of human en-dometrial cancer cell line induced by estrogen.Methods Human endometrial cancer cells,ishikawa were cultured,and then treated with different concentrations of estrogen or saralasin.Cell proliferation was examined using MTT,and cell cycle and apoptosis were determined us-ing flow cytometry.Results Saralsin inhibited the proliferation of ishikawa cells.After treated with saralasin at 10 min after estrogen induc-tion,cycle was arrested at G1/G0 phase and S phase was reduced in ishikawa cell with significant difference between treatment group and control group.An increase in early apoptosis and late apoptosis occurred at 10min after treatment.Conclusion Saralsin inhibits endometrial cancer cell ishikawa proliferation,induces apoptosis and accumulation in G1/G0 phase in vitro.Therefore,saralsin may be helpful for the treat-ment of endometrial cancer.

The clinical supervision of counseling and psychotherapy have a closely connection with clinical employment.Leading the way in clinical supervision,many clinical supervision models has been extrapolated by theories of various psychotherapy,relating to psychoanalysis theory,person-centered approach theory,cognitive and behavior psychotherapy theory,system theory,structuralism theory,and so on.Recently,especial,different clinical supervision models have to expand beyond psychotherapy,such as developmental model of supervision and society role model of supervision.The study has summarized clinical supervision models of the field,in order to offer literature index and concept frame.

Castration-resistant prostate cancer is a fatal disease with rapid progress. This malignancy usually presents with metastasis and poor prognosis. This type of disease also often causes 100%mortality, of which the median survival time is less than 20 months. Thus, treatment of castration-resistant prostate cancer remains challenging, and the underlying mechanisms of this cancer have yet to be identified. Several new therapies for castration-resistant prostate cancer have been proposed, such as androgen receptor antago-nists, immunotherapeutic drugs, taxanes, antiangiogenic agents, radionuclides, and bone-targeting drugs. These therapies can im-prove the survival time of patients. The advances in the treatment of castration-resistant prostate cancer are briefly reviewed in this ar-ticle.

OBJECTIVE:To determine the dissolution of puerarin and 2,3,5,4'-tetrahydroxy-stibene-2-O-?-D-glycoside in Xinnaokang capsules.METHODS:The contents of puerarin and 2,3,5,4'-tetrahydroxy-stibene-2-O-?-D-glycoside were determined by HPLC and the dissolution rates of puerarin and 2,3,5,4'-tetrahydroxy-stibene-2-O-?-D-glycoside in Xinnaokang capsules were determined by basket-rolling method.RESUTLS:With water as releasing media,at a rotation rate of 100 r?min-1,the dissolution rate at 45 min was above 75% of the labeled amount.CONCLUSION:The method can be used for evaluation and control of the internal quality of Xinnaokang capsules.

Objective To apply laparoscopy-assisted small incision surgery in complicated urological operation, and to summarize our experience. Methods We retrospectively analyzed the clinical data of 9 patients with renal tumors (3 with solitary kidney tumors, 2 with unilateral multiple tumors, 1 with bilateral tumors, and 3 with contralateral renal insufficiency), 3 patients with renal pelvic ureteropelvic junction obstruction (UPJO) and multiple stones, and 1 patient with distal ureteral tumor. All patients underwent laparoscopy-assisted small incision surgery in our department from Aug. 2007 to Jun. 2011. The key steps of the surgery were discussed and the clinical experience was summarized. Results (1) For 9 patients with renal tumors, the incision was 4-6 cm in length, the cold ischemia timewas (15 ±4) min, and the operation time ranged from 90 to 180 min. Drainage tubes were removed within 48 h after operation. No patient had bleeding, urinary fistula or other serious complications. (2) For the 3 patients with UPJO, the incision was 4-5 cm in length and the operation time was 110 to 190 min. Drainage tubes were removed within 3-5 d after operation. No patient had bleeding, urinary fistula or other serious complications. No patient had fever or back pain after removal of double-J stents. Intravenous pyelography showed no hydronephrosis and the renal structure was normal. (3) For the patient with ureteral tumor, the drainage tube was removed 3 d after operation and there was no complication. No patients in the present study used analgesic pump or analgesic medications after surgery. Conclusion Laparoscopy-assisted small incision surgery can reduce the length of incision, minimize injury, and protect renal function. Besides, the method is safe and capable of handling complicated situations, especially suitable for patients who are difficult to receive total laparoscopic surgery.

To apply laparoscopy-asiisted small incision surgery in complicated urological operation, and to summarize our experience. Methods We retrospectively analyzed the clinical data of 9 patients with renal tumors (3 with solitary kidney tumors, 2 with unilateral multiple tumors, 1 with bilateral tumors, and 3 with contralateral renal insuffieiency), 3 patients with renal pelvic ureteropelvic junction obstruction (UPJO) and multiple stones, and 1 patient with distal ureteral tumor. All patients underwent laparoscopy-assisted small incision surgery in our department from Aug. 2007 to Jun. 2011. The key steps of the surgery were discussed and the clinical experience was summarized. Results (1) For 9 patients with renal tumors, the incision was 4-6 cm in length, the cold ischemia time was (15±4) min, and the operation time ranged from 90 to 180 min. Drainage tubes were removed within 48 h after operation. No patient had bleeding, urinary fistula or other serious complications. (2) For the 3 patients with UPJO, the incision was 4-5 cm in length and the operation time was 110 to 190 min. Drainage tubes were removed within 3-5 d after operation. No patient had bleeding, urinary fistula or other serious complications. No patient had fever or back pain after removal of doubie-J stents. Intravenous pyelography showed no hydronephrosis and the renal structure was normal. (3) For the patient with ureteral tumor, the drainage tube was removed 3 d after operation and there was no complication. No patients in the present study used analgesic pump or analgesic medications after surgery. Conclusion Laparoscopy-assisted small ineieion surgery can reduce the length of incision, minimize injury, and protect renal function. Besides, the method is safe and capable of handling complicated situations, especially suitable for patients who are difficult to receive total laparoscopic surgery.

Objective To investigate the role of subdural puncture(SDP)in the diagnosis and treatment of subdural fluid collection in young children with purulent meningitis.Methods Totally 207

OBJECTIVE:To promote the rational drug use in our hospital.METHODS:The in-patient and out-patient drug use in our hospital was monitored and analyzed.RESULTS:The income from pharmaceuticals accounted for 38% of the total income in our hospital and the drug use tend to be more rational after the practice of dynamic monitoring on drug use.CONCLUSION:Dynamic monitoring on drug use played a positive role on the rational use of drugs meanwhile it brings the proportion of drug consumption under effective control.

BACKGROUND: High blood lipid can cause a series of cardiovascular and cerebrovascular disease, which has been commonly accepted.OBJECTIVE: To probe into the rule of changes of blood lipid of the elders in aging process.DESIGN: Cross section survey.SETTING: Department of Nutrition, Shanghai Huadong Hospital. PARTICIPANTS: Totally 605 employees who took part in body examination and participated this experiment voluntarily from July 2000 to August 2004, were recruited, including 541 males and 64 females, 91 patients ＜ 70 years , 514 ≥ 70 years, 271 patients having the history of coronary heart disease, 83 of malignant tumors, and 251 of other diseases.METHODS: Blood lipid level of totally 605 who accepted physical examination was detected, at the same times, the disease history and common physical examination status were inquired. Venous blood of the patients was drawn after fasting over 12 hours, separating serum on the morning of the same day. Total cholesterol and triacylglycerol of the blood were detected with enzyme assay by using Automated Biochemistry Analyzer. Total cholesterol: 3.6-6.5 mmol/L; triglyceride: 0.4-1.8 mmol/L were considered as normal level, Total cholesterol: ＞ 5.72 mmol/L;Triglyceride: ＞1.72 mmol/L were considered as abnormal criteria.MAIN OUTCOME MEASURES: Comparison of the blood lipid index of the subjects of different age, disease type and gender RESULTS: Totally 605 elders were involved and all data of them was enthe blood lipid index of the subjects of different age: Peak of total cholesterol appeared at 70 to 74 years, then decreases gradually, the total cholesterol level of the subjects of 70 to 74 years was significantly higher than that at other age (P ＜ 0.05). While Triglyceride level had the trend of going up with growing older, except that of 75-79 ages group slightly went eases: The level of total cholesterol and triglyceride of the subjects in the malignant tumor group was lower than that of other disease groups, level of triglyceride of the subjects in the coronary heart disease was higher than detection rate of blood lipid of the subjects: Detection rate of total cholesterol was 15.5%, while that of triglyceride was 35.9%. There was no significant difference of the detection rate between male and female subjects (P ＞ 0.05).CONCLUSION: Change of blood lipid metabolism of elder with aging is related to the hormone change of different genders and disease types.

Objective To evaluate 5 drug sensitivity tests in detecting carbapenem resistance of KPC-possessing Enterobacteriaceae.Methods Thirty-six Enterobacteriaceae isolates were collected from the First Affiliated Hospital,Cofiege of Medicine,Zhejiang Univeristy,including 32 of Klebsiella pneumoniae,1 of Escherichia coli,1 of Citrobacter freundii,1 of Klebsiella oxytoca,and 1 of Serratia marcescens,and the cacbapenemase producing was confirmed with PCR.Resistance to imipenem,meropenem and ertapenem was determined by agar dilution and disk diffusion tests,that to imipenem and meropehem was determined by ATB with G-5 panel,that to imipenem by BD Phoenix 100 with NMIC-109 panel,and that to imipenem and ertapenem by VITEK 2 Compact with AST-GN13 card.Results The resistance to imipenem determined by ATB,BD Phoenix100,VITEK 2 Compact,agar dilution and disk diffusion tests were 13.9%(5/36),11.1%(4/36),13.9%(5/36),22.2%(8/36)and 69.4%(25/36).ATB,agar dilution and disk diffusion tests reported 22.2%(8/36).55.6%(20/36)and 47.2%(17/36)as resistant to meropenem.VITEK 2 Compact,agar dilution and disk diflusion tests reported 69.4%(25/36),77.8%(28/36)and 88.9%(32/36)as resistant to ertapenem.The MICs of ertapenem determined by VITEK 2 Compact and agar dilution were≥2 μg/mL.Conclusion The sensitivity of agar dilution and agar disk diffusion is higher than other automatic methods in the identification of KPC-mediated carbapenem resistance,and ertapenem is a more sensitive indicator than mempenem and imipenem for screening KPC-possessing isolates.