Objective To compare the safety and efficacy of ibutilide and amiodarone in converting post-coronary artery bypass grafting (CABG) new-onset atrial fibrillation (AF) to sinus rhythm. Methods This study was a prospective, singleblinded, randomized clinical trial. Totally 100 patients who met the inclusion criteria were enrolled and were intravenously treated with ibutilide (group A) or amiodarone (group B). In group A, Ibutilide was administered at a dose of 1 mg in 10 min, which was repeated after an interval of another 10 min if the arrhythmia was not converted; ibutilide was not given anymore no matter the conversion was achieved or not. In group B, amiodarone was administered at a dose of 5 mg/kg for the first 30 min, followed by 1 200 mg in the next 24 h; amiodarone was not given anymore no matter the conversion was achieved or not. Immediate conversion rate, conversion time and post-operative recurrence rate within 30 days were compared between the two groups. Results No severe adverse reactions were observed in both groups. The immediate conversion rate of group A was significantly higher than that of group B (82% vs 58% , P<0.01) , and the conversion time was significantly shorter than that of group B (66 min vs 660 min, P<0.01). The post-operative recurrence rates were not significantly different between the two groups within 30 days after operation (7.3% vs 6.9%, P>0.05). Conclusion Ibutilide and amiodarone are both safe in converting post-CABG new-onset AF to sinus rhythm, and ibutilide is more effective, more rapid in functioning and with a higher efficacy.

Objective: To develop a novel regulable sutureless aortic arch prosthesis and to apply it in an animal experimental study. Methods: The arch skeleton of the prosthesis was made of tandem Z-shape NiTiNOL wire; the branch skeleton was made of laser-cut NiTiNOL tube; and the whole skeleton was coated with thin ePTFE film. The blood vessel was anastomosed by direct ligature, needing no manual suturing. The prosthesis was applied in swine aortic arch operations under the bypass condition. The practicality for surgery and the feasibility of anastomosis of the prosthesis were assessed. Results: Aortic arch operations were successfully performed in 6 of the 8 experimental animals. The prostheses were easy to use, and the mean bypass time was only 10 min. The blood loss of the anastomoses was less than 100 ml within 8 h postoperatively in 5 animals; one had more blood loss due to prosthesis mismatch. Conclusion: The novel regulable sutureless aortic arch prosthesis has satisfactory practicality for surgery and reliable anastomosis, making it promising in future clinical application.

Objective: To evaluate the role of amiodarone in preventing atrial fibrillation after coronary artery bypass grafting (CABG). Methods: Totally 184 patients receiving CABG under cardiopulmonary bypass (CPB) were randomly divided into amiodarone group (n=94) and placebo group (n=90), and patients in each group were further divided into ≥ 60 years and < 60 years subgroups. Patients in amiodarone group received routine drugs + amiodarone and those in placebo group received routine drugs + placebo. Results: The overall incidence of postoperative AF (paroxysmal and persistent) was significantly higher in the placebo group compared with the amiodarone group (32. 2% vs 6. 4%, P<0. 01, OR=7.0). The incidences of postoperative AF were similar in patients aged <60 years between the placebo group and the amiodarone group, but were significantly different in patients aged ≥ 60 (P<0.05 or P<0.01). The postoperative AF incidence was significantly different between 2 age subgroups (P<0.05 or P<0.01) in the placebo group, but not in amiodarone group. Conclusion: Amiodarone can effectively prevent post-operative AF, especially for patients aged ≥ 60 years. Amiodarone should be recommended to patients receiving CABG who aged ≥ 60 years.

Cardiomegaly ; genetics ; Gene Expression Regulation ; Humans ; MicroRNAs ; biosynthesis

Objective To investigate the effect of propofol on nNOS expression after focal cerebral ischemia-reperfusion in rats and the possible mechanism of protective effect of propofol on brain. Method Seventy-eight male Wistar rats, weighting 250 ~ 300 g, were randomly divided into 3 groups:(1)Sham operation group (S group, n=6) was performed with scham operation; (2) Ischemia-reperfusion group (group I-R, n=36) was subjected to 2-hour right middle cerebral artery occlusion and then reperfusion was followed, saline (1 mg/kg) was injected into the right lateral cerebral ventricle using microsyringe before reperfusion;(3) Propefol group (group P, n=36) was injected with propofol (1mg/kg) into the right lateral cerebral ventricle using microsyringe right after ischemia. Group I-R and group P were divided into 3 subgroups according to the reperfusion time: 1 h, 3 h and 6 h. The neurological function of all rats were tested before reperfusion. The cerebral infarction area of the whole brain was calculated with TIC staining (n=6). The pathological change of brain was observed from HE staining (n=6) and the nNOS protein expression was obtained by immuno- histochemical method (n=6). Results Compared with I-R group, the neurological function was better in group P(P

0.05).⑥ The incidence of MSI at D3S1234,D3S4103,D3S1300 in recurring cases was 83.33 %,but the ratio of MSI inprimary cases was 30.77 %,the former was remarkablyhigher than the latter(P=0.004).CONCLUSION ①Microsatellite analysis showed that both LOH andMSI of FHIT gene existed in laryngeal carcinoma andhypopharyngeal carcinoma,the former was morecommon.② FHIT gene participates the developmenof laryngeal carcinoma and hypopharyngeal carcinoma and may be one of the candidate tumor suppressor genes.③ MSI of FHIT gene may be correlated with recurrence of laryngeal and hypopharyngeal carcinoma.

Objective To dynamically the preparation process of Huangqi injection and to verify the rationality and existing problems of the process. Methods The preparation was made by the current standard (WS3-B-3335-98) issued by Ministry of Health, and the solid amount of the key processes were measured. The HPLC separation was performed on a Agilent Zorbax Bio-C18 reversed-phase column (250 mm × 4.6 mm, 5μm) in gradient mode of acetonitrile-water with UV detection at 254 nm. The column temperature was kept at 25 ℃, and the flow rate of mobile phase was 1.0 ml/min. Results Solid amounts of different operations of Huangqi injection were measured accurately. The content of the third water extracts was only 6.1%, and the changes of HPLC pointed the content of the 12 peaks of the second peaks decreased obviously. Conclusion The technological rationality of Huangqi injection need to be verified and optimized, the dynamic analysis of HPLC describes the changes of chemical constituents of Huangqi injection qualitatively, which also provides a reference value for establishing its fingerprint.

Objective To investigate the effect of miR-214 on the invasive capacity of esophageal squamous carcinoma cell line Eca109, and to explore the possible molecular mechanism. Methods We prepared miR-214 double-stranded mimic and transfected it into Eca109 cells with Lipofectamine 2000. Eca109 cells transfected with nonsense miRNA mimics were taken as control. The expression of mature miR-214 was determined by qPCR. The capacity of cell invasion was determined by Matrigel-coated Transwell assay. E-cadherin protein expression and the percentage of E-cadherin positive cells were examined by Western blotting analysis and flow cytometry, respectively. Results The expression level of mature miR-214 in the miR-214 mimic transfection group was significantly higher than that in the control group 48 h after transfection (P<0.01). Eca109 cells transfected with miR-214 mimic showed a significantly lower cell invasive capacity compared to that of cells transfected with control miRNA mimic (P<0.05). Moreover, E-cadherin protein expression and the ratio of E-cadherin positive cells in miR-214 mimic transfection group were both significantly lower than those in the control group (P<0.05). Conclusion Our data suggest that miR-214 may inhibit the invasive capacity of esophageal squamous carcinoma cells by repressing the epithelial-mesenchymal transition.

Benzazepine is a kind of fused ring structure, which is composed of nitrogen-containing seven-membered ring and benzene ring. The introduction of benzazepine scaffolds into compounds can not only adjust the physicochemical properties, maintain or enhance the biological activities of the compounds, but also improve the pharmacokinetic properties, increase the brain permeability, and reduce the toxicity of hERG of the compounds, which is one of the privileged scaffolds for rational design and structural optimization of drug molecules. Benzazepine scaffolds can be constructed by different synthetic methods such as Dickmann condensation reaction, Mitsunobu reaction, Pictet-Spengler reaction, CMD reaction, multicomponent reactions (MCRs), metal catalysis reactions and asymmetric catalysis etc., which play an important role in enriching the structure diversity of drug molecules.

This article analyzed the medical records of a patient with 90% TBSA unhealed wound accompanied with wound sepsis 50 days post burn (PBD) and to discuss the ideal strategies of treatment for such patients in such condition. This was a 24-year-old male patient suffering from flame burn with 95% TBSA wound and severe inhalation injury. Meek skin grafting with autologous scalp was performed once to the thoracic and abdominal regions; intermingled skin grafting of autologous scalp microskin and large sheet of allograft was performed twice to the limbs within PBD 31. The patient was transferred to our hospital on PBD 50 with 90% TBSA wound unhealed, leaving a vast amount of necrotic tissue and allografts. Furthermore, he was complicated by sepsis, pulmonary infection, and gastric ulcer. Debridement and allogenic skin grafting were performed on the first day after hospitalization. When the condition of wounds was improved, transplantation of a large sheet of allogenic skin with inlaid small pieces of autologous skin, intermingled skin grafting of autologous and allogenic skin, and small pieces of autologous skin grafting were performed. Because of the shortage of donor area, the exposed wounds were temporarily covered with allogeneic skin. Epidermal growth factor was used to promote the healing of autologous skin donor site and deep partial-thickness burn wound. Autologous skin grafting was performed whenever source of healthy skin was available. Systemic use of effective antibiotics, nutritional support and therapy, and other comprehensive measures also contributed to the success of treatment of this patient suffering from wound sepsis. The patient was cured and discharged on PBD 145.
Adult ; Burns ; complications ; therapy ; Humans ; Male ; Sepsis ; complications ; therapy ; Skin Transplantation ; Wound Healing