1.The current situation and factors impacting nursing soft skills among student nurses
Ronghua MA ; Tongmei ZHU ; Qing PAN ; Mei AI
Chongqing Medicine 2015;(28):3971-3974
Objective To investigate and analyse the current situation and the factors impacting nursing soft skills in order to provide targeted advice to improve students′ nursing soft skills .Methods Totally 1 000 student nurses were investigated by the nursing soft skills scale and the self‐designed questionnaire .Results First ,there was no differences between the nursing soft skill of students and the national new nurses(P> 0 .05) .But the students′ organizational management and cognitive skills were lower than that of national new nurses(P< 0 .05) .Second ,class leader ,familiarity and fancy for nursing science were the main factors impac‐ting nursing soft skills(P< 0 .05) .Third ,the students′ organizational management and cognitive skills had an increasing trend fol‐lowing the extent of familiarity and fancy for nursing science(P< 0 .05) .Conclusion Education management institution and nursing schools should try to make some reform mearsure to improve students′ nursing soft skills .
2.Analysis of immunophenotype characteristics in 109 cases of B lineage chronic B lymphoid leukemia.
Ai-qing WANG ; Mei-Ju GENG ; Ming-qing ZHU ; Li CHEN
Journal of Experimental Hematology 2011;19(6):1374-1377
This study was aimed to investigate the immunophenotypic characteristics of 109 cases of B-cell chronic lymphoid leukemia (B-CLL) so as to provide evidences for the diagnosis and therapy of B-CLL, and for the detection of the minimal residual disease and its prognosis. Immunophenotyping was performed in 109 patients of B-CLL by two/three color multiparameter flow cytometry analysis using a panel of monoclonal antibodies. The results showed that in 109 cases of B-CLL, all cases expressed CD19, the positive ratios of other B lineage antigen such as CD20, CD22 and CD23 were 95.40%, 94.50%, 86.20% respectively. None of the B-CLL cases expressed CD10. The expression ratio of FMC-7 and CD38 in 105 cases of B-CLL were 28.60% and 36.20%. Among the B-CLL cases the CD5(+) cells amounted to 86.23%, CD5(-) cells amounted to 13.76%, ZAP-70 protein was expressed in 12 out of 50 patients. It is concluded that immunophenotypic data are very useful for the diagnosis and detection of minimal residual disease of B-CLL, and the relationship between the immunophenotypic characteristics and the prognosis of B-CLL needs further study.
Adult
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Aged
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Aged, 80 and over
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Female
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Flow Cytometry
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Humans
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Immunophenotyping
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Leukemia, Lymphocytic, Chronic, B-Cell
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diagnosis
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immunology
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Male
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Middle Aged
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Neoplasm, Residual
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diagnosis
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immunology
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Prognosis
3.Intervention effects of qingre jiangya capsule on brain hippocampus of spontaneously hypertensive rats based on metabonomic research.
Hai-Qing JIANG ; Lei NIE ; Yun-Lun LI ; Miao-Miao WANG ; Mei ZHU ; Wen-Qing YANG ; Xin-Ya ZHANG
China Journal of Chinese Materia Medica 2014;39(1):134-139
Thirty SHRs were obtained randomly to hypertension, model group, captopril group and Qingre jiangya capsule group. Ten Wistar rats were used as control group. The hippocampus tissue was removed to explore the damage of spontaneously hypertensive rats (SHR) and the protective effect of Qingre jiangya capsule after continuously administered for 14 days. And then the data were processed by principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA). The research results revealed captopril group was significantly different from the other three groups. The classification of other three groups is also very clear after captopril group removed. This suggested that Qingre jiangya capsule could improve the overall metabolism compared with captopril. Four metabolites were identified: dimethylglycine, glycerophosphocholine, aldosterone and noradrenaline. Hypertension hippocampus damage may mainly be expressed in tyrosine metabolism, aldosterone-regulated sodium, vascular smooth muscle contraction reabsorption, and Qingre jiangya capsule could reverse the hippocampus tissue damage of SHR.
Animals
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Capsules
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pharmacology
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Drugs, Chinese Herbal
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pharmacology
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Hippocampus
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drug effects
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Hypertension
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drug therapy
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Male
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Rats
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Rats, Inbred SHR
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Rats, Wistar
4.Expression of cyclin-dependent kinase CDC2 and its significance in malignant progression of gliomas.
De-zhong ZHAI ; Qiang HUANG ; Qing ZHU ; Hong-mei HUO ; Jun DONG ; Zhi-yuan QIAN ; Ai-dong WANG ; Qing LAN
Chinese Journal of Pathology 2007;36(3):196-197
Adolescent
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Adult
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Aged
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Aged, 80 and over
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Animals
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Bone Marrow
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metabolism
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Brain
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metabolism
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Brain Neoplasms
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metabolism
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pathology
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CDC2 Protein Kinase
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metabolism
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Cell Line, Tumor
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Child
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Child, Preschool
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Female
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Gene Expression Regulation, Neoplastic
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Glioma
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classification
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metabolism
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pathology
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Humans
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Male
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Mice
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Mice, Nude
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Middle Aged
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Neoplasm Transplantation
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Neoplastic Stem Cells
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metabolism
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Young Adult
5.The construction and identification of Fas-targeting siRNA-expressing plasmid
Suhu LIU ; Wanggang ZHANG ; Mei ZHANG ; Qing ZHU ; Wei TIAN ; Jingxi SUN
Journal of Xi'an Jiaotong University(Medical Sciences) 1981;0(02):-
Objective To construct Fas-targeting siRNA-expressing plasmid, and explore the significance of Fas inhibition in the treatment of acute aplastic anemia. Methods U6 promoter cassette and siFas sequence were obtained by PCR method, and cloned into modified pcDNA3.1 to produce plasmid pU6-siFas, which was transfected into P815 cells with limpofectin 2000, and then Fas expression was detected by immunohistochemistry. Results The plasmid pU6-siFas could efficiently reduce the expression of Fas and confer G-418 resistance in P815 cells. Conclusion The successful construction of the siRNA expressing plasmid will facilitate the application of RNA interference technique, and lay foundation for further studies of the therapeutic effect of Fas inhibition on acute aplastic anemia.
6.Study on intra-retinal layers imaged with ultra - high resolution optical coherence tomography
Su-Zhong, XU ; Sheng-Hai, HUANG ; Qing-Kai, MA ; De-Xi, ZHU ; Mei-Xiao, SHEN
International Eye Science 2014;(8):1494-1497
To evaluate the automated segmentation algorithm for detection of intra - retinal layers to process images obtained from ultra- high resolution optical coherence tomography ( OCT ) . Graph theory and the shortest path search based on dynamic programming were applied to automatically segment the 8 intra - retinal layers. We experimentally verified the accuracy and reliability of the algorithm. The results showed that the intra-retinal layer boundaries between automated and manual segmentations matched well. The algorithm successfully segmented the intra- retinal layers in glaucoma, high myopia, and retinitis pigmentosa patients. The proposed automatic segmentation for intra-retinal layers provides a promising tool for quantitative analysis in clinical diagnosis and treatment.
8.Primary mesenchymal chondrosarcoma of the lung.
Jian GENG ; Yan-qing DING ; Li-fei LIU ; Mei-gang ZHU ; Hui-xia HAN ; Jun-jie CAI
Chinese Journal of Pathology 2005;34(5):317-318
12E7 Antigen
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Antigens, CD
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metabolism
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Bone Neoplasms
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metabolism
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pathology
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surgery
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Cell Adhesion Molecules
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metabolism
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Chondrosarcoma, Mesenchymal
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metabolism
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pathology
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surgery
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Diagnosis, Differential
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Hemangiopericytoma
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pathology
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Humans
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Lung Neoplasms
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metabolism
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pathology
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surgery
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Male
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Middle Aged
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Pneumonectomy
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methods
9.Effects of qijingmingmu soup on the expression of matrix metalloproteinases in the conjuntival fibroblasts of conjunctivochalasis
Min-hong, XIANG ; Yi-jie, LI ; Xing-ru, ZHANG ; Qing-song, LI ; Zhu-mei, HAN ; Long, ZHANG
Chinese Journal of Experimental Ophthalmology 2013;31(10):940-943
Background Our previous study determined that expressions of matrix metalloproteinases (MMPs) and tissue matrix metalloproteinase inhibitors (TIMPs)change in the conjuntival fibroblasts of conjunctivochalasis in vitro.To seek a suitable drug is very important in the prevention and treatment of conjunctivochalasis.Objective This study was to explore the effect of qijingmingmu soup on the expressions of MMPs and TIMPs in human conjunctival fibroblasts of conjunctivochalasis.Methods Twenty-four SD rats were randomized into two groups.Qijingmingmu soup was administration gastrically for consecutive 3 days,and normal saline solution was given in the same way in the control group.The blood was collected from aortaventralis and drug serum was prepared.Human conjunctival samples were obtained during the surgery of conjunctivochalasis relaxation and cultured in the DMEM containing 10% fetal bovine serum,20%,15%,10%,5% of drug serum and 8 ml/L epidermal growth factor(EGF) was added into the medium respectively.Enzyme-linked immunosorbent assay(ELISA)was used to detect the expressions of MMP1,MMP3,TIMP1 and TIMP3in conjunctival fibroblasts.Results Cultured cells grew well with the fusiform shape and showed the positive response for vimentin.The expression value of MMP1 (A value)in the cells was declined after administration of qijingmingmu soup.A significant difference was found in the expression of MMP1 among the control group,20%,15%,10%,5% drug serum groups and EGF group(F=466.664,P<0.05),and that in the 10% ([9.92±0.14] mg/L) and 20% ([11.87 ±0.11] mg/L) drug serum groups was significantly lowed in comparison with the control group([16.31±0.10] mg/L)(t=99.974,87.394,P<0.05).The expression value of the MMP3in the cells in the various drug serum groups,EGF group and the control group was significantly different(F=158.168,P<0.05),with a lower value in the 20% drug serum group compared with the control group ([3.50±0.03] mg/L vs.[4.44 ± 0.11] mg/L) (t =21.991,P < 0.05).Also,the significantly different expressing value of TIMP1 was seen among all the groups (F=183.508,P<0.05),and expressing value of TIMP1 in the 15% drug serum group was(1.88±0.06)mg/L,which was lower than(3.20±0.32) mg/L of the control group(t=10.353,P<0.05).Furthermore,the expressing value of the TIMP3 in the cells was significantly different among the various groups(F=54.503,P<0.05),and that of the 20% drug serum group was (1.743±0.065)mg/L and it was significantly higher than (1.54 ± 0.05) mg/L of the control group (t =5.046,P =0.004).However,the expressing value of TIMP3of the 15%,10% and 5% drug serum groups was lower than that of the control group,respectively all at(P<0.05).Conclusions Qijingmingmu soup drug serum at the concentration of 20% can down-regulate the expressions of MMP1,MMP3,TIMP1 and up-regulate the expression of TIMP3 in human conjunctivochalasis bulbar conjunctival fibroblastsin vitro,which probably plays preventive and therapeutic effects on conjunctivochalasis.
10.Expression of caspase-9 affected by AG on retina of rats with chronic IOP elevation
Qing-Zhu, NIE ; Qian, SHA ; Ying-Shuang, WANG ; Dong-Mei, GUI ; Zhi-Li, LIU ; Dian-Wen, GAO
International Eye Science 2008;8(7):1299-1303
AIM: To study caspase-9 expression on rat retina in the process of chronic elevation of IOP and the changes with the application of amino guanidine (AG), thus to investigate potential protective function of AG to rat retina with chronic elevation of IOP.METHODS: Immunohistochemistry, RT-PCR and Western blot were used to observe retinal morphology and expression of caspase-9 at different time points of rat with chronic IOP elevation, both affected or not affected by the application of AG.RESULTS: Compared with control group, as time passed retina of experimental group gradually had detectable morphological changes. On 21st day of chronic IOP elevation, retinas became thinner and the quantity of retinal ganglion cells (RGCs) decreased; caspase-9 expression increased, consistent with the morphological changes. The group using AG presented relatively smaller morphology changes and less expression of caspase-9.CONCLUSION: Apoptosis-related gene caspase-9 played a part in the process of chronic IOP elevation; AG protects retina by down-regulating expression of caspase-9.