Objective To investigate the role of subdural puncture(SDP)in the diagnosis and treatment of subdural fluid collection in young children with purulent meningitis.Methods Totally 207

Objective To evaluate the clinical application of retroperitoneal laparoscopic adrenal gland sparing adrenalectomy in the treatment of primary hyperaldosteronism with adrenal adenoma. Methods From 2001 to 2006, clinical data of 196 primary hyperaldosteronism with adrenal adenoma patients (78 males and 118 females with mean age of 41±12, ranging 16-69 years old) confirmed during operation were retrospectively analyzed. All cases were with preoperatively high plasma aldo-sterone, low plasma renin, hypokalemia and arterial hypertension. Results Retroperitoneal laparo-scopic adrenalectomy were successfully completed in all cases. No major complication occurred. of them, partial adrenaleetomies were performed in 51 cases and enucleationa of adrenal adenoma were performed in 145 cases. The operative time was ranged from 15 to 87 min (MD=33 min) and the esti-mated blood loss was ranged from 5 to 200 ml (MD=20 ml). There was no case accepted transfusion. The length of hospital stay was ranged from 2 to 5 d (mean 2.7±1.3 d). All the 196 cases were fol-lowed up with the range from 6 months to 3 years (mean, 1.8 years). Postoperatively, kalemia was normalized in all cases, blood pressure was normalized in 168 cases (85.7%). The abnormal blood pressure cases were under control with anti-hypertension drugs. The CT scan showed adrenal glands were normal. Serum test showed normal hormonal levels. The serum potassium levels were in normal range in all cases. One cases with abnormal post-operative blood pressure had accepted second surgery to remove the adrenal adenoma and the blood pressure turned to be in normal range afterwards. Con-clusion Retroperitoneal laparoscopic adrenal gland sparing adrenalectomy is a safe and feasible tech-nique and is the first choice in the management of primary hyperaldosteronism with adrenal adenoma.

Amino Acid Sequence ; Animals ; Birds ; Cell Transformation, Viral ; Herpesvirus 2, Gallid ; genetics ; physiology ; Marek Disease ; virology ; Models, Biological ; Molecular Sequence Data ; Oncogene Proteins, Viral ; genetics ; physiology

?As a globally recognized irreversible blindness disease, glaucoma can lead to pathological intraocular hypertension, loss of optic ganglion cells and axonal progressive, more and more deep optic cup, and the expanded visual field defect. Various researches show that excitatory amino acid toxicity, oxidative damage, apoptosis, intracellular Ca2+overloading etc. , pathogenic factors are all involved in the occurrence and development of glaucoma. Now, a variety of clinical drugs and operation treatment are applied to control the glaucoma progress. Further more, there are many new drugs and methods in the process of development. This is an article on the current anti-glaucoma drugs.

Objective To observe the ultrasonographic features of local chest wall tumor recurrence after mastectomy for breast cancer and its clinical and histopathological characteristics. Methods The ultrasonographic features, clinical and histopathological characteristics of 27 patients with local chest wall tumor recurrence after mastectomy confirmed histopathologically were retrospectively reviewed. Results The disease-free intervals of twenty-seven patients ranged from 3 to 129 months [mean (31.9±31.4) months]. Most of the recurrence(18/27, 66.7%) occurred within 3 years after mastectomy. The clinical manifestations were:7 cases (7/27, 25.9%) with regional skin redness and swelling, red rash or ulceration on chest wall associated with or without palpable mass, 20 cases(20/27, 74.1%) with chest wall palpable masses without obvious skin change. On ultrasonography, 2 cases showed diffuse inifltrative type with ill-deifned inhomogeneous hypoechoic lesion and skin thickening. And twenty-ifve cases(43 lesions) showed mass type with a lesion size range of 5.4-114.7 mm [mean (24.4±21.6) mm]. Among them, 32 lesions were located near to the operation incision scar, 36 involved muscle layer, 38 were hypoechoic, 31 had irregular shape, 24 had indistinct margin, and 31 had blood lfow signal. In addition, calciifcation, halo, and taller-than-wide shape were absent in all 43 lesions. Conclusions The tumor recurrence often occurred within 3 years after mastectomy in high-risk patients. Ultrasonographic feature of chest wall recurrent lesion is of great value in the diagnosis.

AIM: To compare the therapeutic effects of transplantation of human umbilical cord mesenchymal stem cells (hUCMSC) through different ways on diabetic mice.METHODS: hUCMSCs were labeled with enhanced green fluorescent protein (EGFP) and luciferase (Luc) reporter gene, and then the cells were transplanted into the diabetic mice through pancreas or tail vein to monitor the migration of the hUCMSCs in vivo.The pathological changes of pancreas tissue sections were determined by HE staining.Weight and blood glucose of the mice were measured dynamically.To compare the therapeutic effects, serum insulin levels were analyzed and glucose tolerance test were also performed.RESULTS: In vivo bioluminescence imaging results showed that the hUCMSCs transplanted into pancreatic capsule was mainly located in the pancreas while the hUCMSCs transplanted through vein tail injection was mainly located in the lung.HE staining illustrated that islet cells presented distinctive boundary and no infiltration of inflammatory cells in pancreatic capsule transplantation group was observed, but a little inflammatory cell infiltration and fibrosis formation in tail vein injection group were seen.A significant decrease in blood glucose level and a significant increase in serum insulin level in pancreas transplantation group were showed as compared with vein tail injection group.CONCLUSION: Transplantation of hUCMSCs through different approaches demonstrates different effects.The transplantation of hUCMSCs into pancreatic capsule is more effective on hyperglycemia reversion, insulin secretion and improvement of beta-cell function than that through tail vein.

Objective:To investigate the expression of human telomerase reverse transcriptase (hTERT) in urine exfoliated cells of benign and malignant urothelial diseases, in an attempt to evaluate the role of detecting hTERT gene expression in cytologic diagnosis of transitional cell carcinoma. Methods: The expressions of hTERT gene were detected by immunocytochemistry staining in 54 patients with transitional cell carcinoma(25 cases with grade Ⅰ, 26 with grade Ⅱ and 13 with grade Ⅲ) and 20 controls, including 10 patients with urinary tract stone and 10 healthy volunteers. The urine exfoliative cells of both groups were also stained by H-E and the results were compared with those of immunocytochemistry.Results: hTERT was detected in 74.1% (40/54) of patients with transitional cell carcinoma and in 5%(1/20) of the controls (P

Objective To investigate the expression of BRCA1 in chemosensitive and chemoresistant ovarian cancer specimens,so as to provide a novel insight into the epigenetic mechanism involved in BRCA1 transcription. Methods Serous ovarian cancer patients(10 chemosensitive and 10 chemoresistant cancer)were enrolled for the study. BRCA1 levels was analyzed by real?time quantitative PCR. The methylation levels of BRCA1 core promoter(sites 1?4)was determined by pyrosequencing. Regression analysis was used to examine the possible relationship between BRCA1 levels and the methylation levels of sites 1?4 in ovarian cancer specimens. Results Compared to chemosensitive ovarian cancer tissues,BRCA1 levels were increased,but the methylation levels of BRCA1 core promoter(sites 1?4)were decreased in chemoresistant ovarian cancer tissues. How?ever,it is interesting to note that only a significant inverse correlation was observed between BRCA1 levels and the methylated levels of site 4 (r=-0.612,P<0.05). Conclusion Our findings imply that the methylation levels of site 4 in the core promoter of BRCA1 may be widely involved in the regulation of BRCA1 expression and chemosensitivity in ovarian cancer.

Objective To explore the clinical characteristics and diagnosis of mycoplasma pneumoniae(MP) pneumonia showing lobar pneumonia.Methods Patients from Jan.2004 to Dec.2005 were selected as researching case,which had samptoms and signs of respiratory in clinic,the chest Xray shown lobar pneumonia,the MP of down respiratory′s secretion detected by polymerase chain reaction(PCR) and MP-IgM of serum detected by indirect hemagglutination were positive.The clinical data of them were reviewed.Results MP pneumonia showing lobar pneumonia was same to streptococcus pneumoniae pneumonia in clinic,but it had some characters:1.majority of the lobar MP pneumonia were children being student;2.the process was long,the samptoms of respiratory was tipical,the continuous fever was few,and the infectious samptoms was not obvious as streptococcus pneumoniae pneumonia;3.the injury to lung was more and tipical;4.the increase of white blood cell,neutrocyte and C-reactive protein were not clearly as to streptococcus pneumoniae pneumonia,but the absorbing of lung′s focal was relatively fast;5.the ?-lactams antibiotic was not effective,but macrolides was effective.Conclusions Clinical characters of MP pneumonia are needed to master,the variety of chest X-ray changes and differentiate from other pneumonia infected by other are needed to notice.

Technologies for the trans-institutional sharing of scientific data were studied with the sharing of nucleic acid sequence data as the study object , such as leaving a flexible space for implementation of the unified standards in working out the criteria for data sharing , implementing regular point to point data sharing and updating in agree-ment of institutional league , and providing multiple data services according to the unified working process by giving considerations to the local demands .The significance of trans-institutional sharing of scientific data under the insti-tutional league model was summarized for the reference in constructing the scientific datasharing platform.