1.The analysis of epidemiological and clinical characteristics of 112 cases patients with adult-measles
Xiao-Hua CHEN ; Jie XU ; Guo-Qing ZANG ;
Chinese Journal of Infectious Diseases 2001;0(03):-
Objective To analyze the clinical data of 112 patients with adult-measles in the Af- filiated Shanghai Sixth People's Hospital and Third People's Hospital of Shanghai Jiaotong University from January to June in 2005,and to evaluate the epidemiologieal and clinical characteristics of adult- measles.Methods To retrospectively analyze the clinical data of 112 patients with adult-measles. Results Totally,112 measles patients were involved in this study,with male 63 and female 49 re- spectively.The gender rate was 1.3:1,average age about 32 years and the proportion of non-resi- dents was 40.18%.The peak of the incidence of disease emerged in April and May,and the positive rate of measle virus IgM antibody in serum was 96.43%.There were 97 patients(86.61%)who had the fever that beyond 39℃and 36 patients(32.14%)who had measle caused viral hepatitis.Conclu- sions There is a trend that the ages of patients that suffered from measle were postponed and the in cidences of disease are increased year by year,which maybe related to the nonstandard inoculation of measle vaccine,or the decrease of IgM antibody levels and so on.Meanwhile,serious toxemie symp- toms and liver damage can be observed.
2.A study of integrin beta3 mRNA expression and its correlation with microvascular density, progression and prognosis of gastric carcinomas.
Zhong-sheng ZHAO ; Guo-qing RU ; Jie MA
Chinese Journal of Pathology 2004;33(1):16-20
OBJECTIVETo investigate mRNA expression of integrin beta3 in gastric carcinoma, its correlation with microvascular density (MVD), growth-pattern, invasion, metastasis and prognosis.
METHODSIn situ hybridization and immunohistochemistry techniques were used to study the expression of integrin beta3 mRNA and CD34 protein in 105 gastric carcinoma specimens.
RESULTSIn situ hybridization revealed a 30% (6/20) positive rate of integrin beta3 mRNA in non-tumor gastric mucosa, which was significantly lower than that of the gastric cancer group (61.0%, 64/105, chi(2) = 8.85, P = 0.037); In infiltrating type cases (70.2%, 40/57), stage III - IV (72.1%, 44/61), lymphatic metastasis (68.6%, 48/70) and distant metastasis (85.7%, 36/42), the positive expression rates were significantly higher than those of the expanding type (chi(2) = 14.97, P = 0.002), stage I - II (chi(2) = 15.21, P = 0.015), non-lymphatic metastasis (chi(2) = 17.89, P = 0.025) and non-distant metastasis (chi(2) = 20.22, P = 0.005; chi(2) = 21.35, P = 0.035); its mean MVD was also significantly higher than that of the expanding type (t = 10.105, P = 0.001), stage I approximately II (t = 5.961, P = 0.001), non-lymphatic metastasis (t = 3.819, P = 0.01)and non-distant metastasis (t = 10.578, P = 0.001; t = 7.882, P = 0.001), respectively; The mean MVD (41.02 +/- 8.55)/0.72 mm(2) of the integrin beta3 mRNA positive expression group was significantly higher than (25.26 +/- 11.25)/0.72 mm(2) of the negative expression group. There was a positive relation between MVD and integrin beta3 mRNA expression in the tumor (r(s) = 0.316, P = 0.001). The mean survival time in cases with positive integrin beta3 mRNA expression or MVD value >or= 39.5 was significantly shorter than that in cases with negative expression or MVD value < 39.5.
CONCLUSIONSIntegrin beta3 expression correlates with enhanced tumor angiogenesis and may play a role in the invasion and nodal metastasis of gastric carcinoma, therefore it may serve as a prognostic marker of gastric cancer.
Adult ; Aged ; Disease Progression ; Female ; Humans ; Integrin beta3 ; genetics ; Male ; Middle Aged ; Neovascularization, Pathologic ; etiology ; Prognosis ; RNA, Messenger ; analysis ; Stomach Neoplasms ; blood supply ; metabolism ; pathology ; Survival Rate
4.Studies on methods of in vitro evaluation of soft rot-resistance in jinxianlian.
Qing-Song SHAO ; Hong-Bo LIU ; Jie GUO ; Yan XU ; Run-Huai HU ; Ming-Yan LI
China Journal of Chinese Materia Medica 2014;39(1):44-47
Compared with living spray method, it focused on the investigation of different inoculation methods, various inoculation concentration and the influence of different seeding age on soft rot-resistance in Jinxianlian. The results showed that (1) Inoculated with dropping connection, the difference of disease index between A. roxburghii and A. formosanus was grate, so that the disease-resistance could be obviously distinguished. (2) When the inoculation concentration was 1.0 x 10(7) cfu x mL(-1), the difference of disease index was relatively obvious and the disease-resistance could be differentiated well. (3) At the moment of 4-month seeding inoculation, a certain difference of the disease index between A. roxburghii and A. formosanus was existed, so, relatively, it could accurately reflect the resistance difference between various species. With the inoculation of dropping connection, A. roxburghii and A. formosanus of 4-month seeding age was put in the bacteria suspension of inoculation concentration of 1.0 x 10(7) cfu x mL(-1). The identification was taken up after 5 days in the incubator under the condition of 14 h daylight and 28 degrees C. The identification result was conformed with that of the living spray method. To investigate the identification method of in vitro evaluation of soft rot-resistance of Jinxianlian so as to provide the foundation for germplasm utilization and excellent cultivars breeding.
Plant Diseases
;
microbiology
5.A case of Shwachman syndrome.
Bao-xi ZHANG ; Xiao-qing ZHAO ; Xiao-li WU ; Wen-jie GUO
Chinese Journal of Pediatrics 2004;42(7):550-550
Abnormalities, Multiple
;
genetics
;
pathology
;
Child, Preschool
;
Chromosome Aberrations
;
Diagnosis, Differential
;
Diarrhea
;
diagnosis
;
etiology
;
therapy
;
Exocrine Pancreatic Insufficiency
;
complications
;
Fever
;
diagnosis
;
etiology
;
Fingers
;
abnormalities
;
Humans
;
Male
;
Pancreatic Diseases
;
etiology
;
Syndrome
6.Confirmation of pipetting performances for fully-open automatic biochemistry analyzers.
Chinese Journal of Medical Instrumentation 2005;29(4):277-282
This paper introduces a kind of evaluation method in pipetting performance on new fully automated biochemistry analyzers by experiments. The performance of sample pipetting volume is confirmed by dye dilution method, the performance of reagent pipetting volume and dummy volume is done by weighing method. Meanwhile, researches and comparative researches on dummy volumes in different conditions have been made, providing valuable reference for clinical applications of automatic biochemistry analyzers.
Automation, Laboratory
;
instrumentation
;
methods
;
Biochemistry
;
instrumentation
;
methods
7.Effects of orthodontic tooth movements on insulin-like growth factors expression in periodontal tissues.
Jie GUO ; Qing ZHAO ; Yang-xi CHEN
West China Journal of Stomatology 2008;26(4):439-442
OBJECTIVETo elucidate the regulatory bio-mechanism of estrogen and local stress on the bone remodeling activities in vivo.
METHODSA Wistar rat experimental model was established to apply orthodontic activation during different stages of estrous cycle. In situ hybridization was used to detect the variation of the insulin-like growth factors (IGF) mRNA expression in the periodontal tissues. The data of each group was analyzed with SPSS 11.0 software package for Student-Newman-Keuls (S-N-K) test and one way ANOVA test.
RESULTSBoth IGF-I mRNA and IGF-II mRNA level expressed in the periodontal tissues were increased by orthodontic activation during various stages of the estrous cycle. But a similar pattern emerged that the IGF-I mRNA level was the lowest in the estrous stage, whereas its level was the highest in the proestrus stage (P < 0.05). No rhythm of IGF-II mRNA expression found during the estrous cycle.
CONCLUSIONIt was concluded that local mechanical stimulation directly enhanced the IGF expression. In contrast to the IGF-II, IGF-I was also influenced by the sex hormone rhythm during the estrous cycle.
Animals ; Estrous Cycle ; Estrus ; Female ; In Situ Hybridization ; Insulin-Like Growth Factor I ; RNA, Messenger ; Rats ; Rats, Wistar ; Somatomedins ; Tooth Movement Techniques
8.A bio-mechanism study of differential orthodontic tooth moving speed during the estrous cycle.
Jie GUO ; Qing ZHAO ; Yang-xi CHEN
West China Journal of Stomatology 2008;26(3):327-330
OBJECTIVEThe purpose of this article was to investigate the changes of the estrogen and insulin-like growth factors (IGF) level induced by orthodontic tooth movements in order to elucidate the bio-mechanism of orthodontic treatments during the estrous cycle.
METHODSA Wistar rat experimental model was established to apply orthodontic activation during different stages of estrous. The serum and periodontal tissue estradiol level were determined by radioimmunoassay and immunohistochemistry. In situ hybridization was also used to detect the variation of the IGF mRNA expression in the periodontal tissues. The data of each group was analyzed with SPSS 11.0 software package.
RESULTSBoth serum and periodontal estradiol level were increased by orthodontic activation during various stages of the estrous. IGF mRNA expression were also enhanced with the same trend. The estradiol and IGF- I level showed in a similar pattern during the estrous cycle and the rhythm was not changed by the orthodontic activation. There was no rhythm of IGF-II expression found in the estrous cycle.
CONCLUSIONIt was concluded that estrogen and IGF were involved in the remodeling activities induced by the orthodontic activation. The speed of the orthodontic tooth movements was closely related to the estrous cycle.
Animals ; Estradiol ; Estrous Cycle ; Estrus ; In Situ Hybridization ; Insulin-Like Growth Factor I ; Rats ; Rats, Wistar ; Tooth Movement Techniques
9.Correlative studies on uPAR receptor mRNA expressions with vascular endothelial growth factor, microvessel density, progression and survival time of gastric carcinomas.
Zhong-sheng ZHAO ; Guo-qing RU ; Jie MA ; Wen-juan XU ; Zhong MENG
Chinese Journal of Pathology 2005;34(5):306-307
Adenocarcinoma
;
blood supply
;
metabolism
;
mortality
;
secondary
;
Adult
;
Aged
;
Female
;
Humans
;
Lymphatic Metastasis
;
Male
;
Microcirculation
;
Middle Aged
;
Neovascularization, Pathologic
;
metabolism
;
pathology
;
Prognosis
;
RNA, Messenger
;
biosynthesis
;
genetics
;
Receptors, Cell Surface
;
biosynthesis
;
genetics
;
Receptors, Urokinase Plasminogen Activator
;
Stomach Neoplasms
;
blood supply
;
metabolism
;
mortality
;
pathology
;
Survival Rate
;
Vascular Endothelial Growth Factor A
;
metabolism
10.Diagnosis and surgical treatment of solid pseudopapillary neoplasm of pancreas
Quanbo ZHOU ; Ning GUO ; Xiaofeng LIN ; Qing LIN ; Rufu CHEN ; Jie WANG ; Jisheng CHEN
Chinese Journal of Hepatobiliary Surgery 2012;18(7):499-502
ObjectiveTo review our experience in the diagnosis and surgical treatment of solid pseudopapillary neoplasm (SPN) of the pancreas which can be used as a reference for other doctors to avoid misdiagnosis and to provide a better treatment.MethodThe clinical,laboratory,radiological,pathological and operative data of 24 patients with SPN of the pancreas operated between February 2001 to December 2009 were collected and retrospectively analyzed.Results23 of 24 patients were female and the mean age was 31 years.The most common clinical presentations were vague abdominal pain and abdominal mass.In most cases,abdominal imaging showed a solid or a solid-cystic mass in the tail or head of pancreas.All patients received surgery.20 of 22 patients who received curative resection were alive with no evidence of tumour recurrence.One patient who had a R1 resection died 42 months after surgery.The remaining patient was alive after a second operation.ConclnsionsSPN of the pancreas is a tumour with low malignancy.A correct diagnosis of SPN of the pancreas is made on its clinical,radiological and histopathological characteristics.Radical surgical resection is the treatment of choice.For patients with an advanced disease,palliative resection is beneficial.