Actins ; metabolism ; Adult ; Diagnosis, Differential ; Endometrial Neoplasms ; metabolism ; pathology ; surgery ; Endometrial Stromal Tumors ; metabolism ; pathology ; surgery ; Female ; Humans ; Hysterectomy ; Leiomyoma, Epithelioid ; metabolism ; pathology ; Melanoma-Specific Antigens ; metabolism ; Perivascular Epithelioid Cell Neoplasms ; metabolism ; pathology ; surgery ; Receptors, Progesterone ; metabolism ; Sarcoma, Clear Cell ; metabolism ; pathology ; Uterine Neoplasms ; metabolism ; pathology ; surgery

Objective In the computer-oriented network management platform,discharged patients with type 2 diabetes was intervened with multi-form follow-up forms,and analyze the different effects at different stages of health education interventions.Methods Based on diabetes management center database follow-up system,various forms of follow-up program was used for 125 patients with type 2 diabetes who discharged from June 2014 to January 2015,they were followed for a period of six months,the self-management behaviors and blood glucose of patients at different stages,15 day,1 month,3 months,6 months were analyzed and compared.Results After discharged 15 days,1 month,3 months,6 months,the compliance to diet,exercise,blood glucose monitoring and medication of type 2 diabetes was statistically significant (x2=8.274-28.634,P＜ 0.05).The number of discharged patients regularly attending health education seminars,screening complications and glycosylated hemoglobin,attending outpatient follow-up program is gradually increased,the difference was statistically significant (x2=37.722-75.000,P ＜ 0.05).2-hour postprandial blood glucose level and compliance rate,fasting blood glucose level control and compliance rate were stable,the difference were no statistically significant (P ＞ 0.05).Conclusions The computerized various forms of follow-up programs can improve the self-management behaviors and maintain a stable glycemic control of discharged patients with type 2 diabetes.

Objective:To study the influence of vitamin C ( VC) on dendritic cells ( DC) ,and detect DC maturation,to provide a feasible method and thought for quickly preparating DC vaccines.Methods:Collected the peripheral blood (about 50 ml) from healthy volunteers,and isolated peripheral blood mononuclear cells with lymphocyte separation medium and obtain DC.With stimulating with different concentrations of VC for (24 h),then washed with PBS,and set up blank control group (V0).The expression of DC surface co-stimulating molecules CD80/86 and HLA-DR, CD40 was detected by flow cytometry.By setting the concentration gradient and time gradient, exciting optimal concentration and stimulating time of VC on DC, and analyzed the reasons of VC promoting DC maturation.Results:VC could effectively stimulate DC,CD80/86 expression had significantly increased contrast to the blank control group (V0).And the experiments show that VC’s best stimulating concentration was 1 mmol/L,and on the third day,the CD80/86 expression rate of VC group was (78.6±4.6) %,and blank control group V0 was (34.1±5.7) %.DC surface HLA-DR expression:VC (56.8± 4.4) %,blank control group V0 (25.4 ±4.7) %,the difference between two groups was statistically significant,P<0.05.CD40 and CD40L expression and results show that VC 2.5 mmol/L group of CD40 expression rate up to (59.3±3.7) %,while V0 group was only (11.1 ±2.4) %,that illustrate VC could significantly regulate CD40 expression on DC surface,but CD40L not reflect.Fluorescence mi-croscope results showed that DC’ s antigen catching ability was also significantly promoted.Conclusion:VC can significantly regulate DC maturity,and may up regulate CD40,thus promoting the express of CD80/86 and HLA-DR.When the concentration is 1 mmol/L,VC expresses the strongest regulation function on DC.

Personnel training marks a key assurance for the healthy and sustainable development of healthcare sector and healthcare institutions in China, rendering it critical importance to include personnel training into performance appraisal. The authors analyzed the connotation and orientation of personnel training index assessment in the performance appraisal at tertiary public hospitals in the country, sorted out the assessment details to be optimized. On such basis, the authors recommended on optimal assessment methodology, combining the gradual improvement of medical personnel training assessment indexes and interval range assessment. These efforts aim at providing references for further improving the scientificity, standardization, homogeneity, as well as vertical and horizontal comparability and operability of personnel training index assessment.

Objective To investigate the protective effect of methylene blue (MB) on blood-brain barrier (BBB) injury after focal cere-bral ischemia-reperfusion in rats. Methods 18 male Sprague-Dawley rats were randomly divided into sham-operated group (n=6), model group (n=6) and MB treatment group (n=6). The left middle cerebral arteries were occluded for 1 hour and reperfused. MB was infused intra-venously immediately after reperfusion (3 mg/kg) and again 2 hours post-reperfusion (1.5 mg/kg), while normal saline was administered in the model group. The sham-operated group was treated as same as the model group without occlusion and infusion. HE staining was used to observe the histological injury in the cortex around the infarcted region 47 hours after reperfusion, while albumin immunohistochemistry was used to evaluate the permeability of the BBB, and immunohistochemistry and double immunofluorescence staining were used to exam-ine the expressions of glial fibrillary acidic protein (GFAP) and aquaporin-4 (AQP-4). Results HE staining showed that cells and blood ves-sels were not intact in the cortex around the infarcted region in the model group and they were better in the MB treatment group. The expres-sions of the albumin, GFAP and AQP-4 were higher in the model group than in the sham-operated group (P<0.01), and were lower in MB treatment group than in the model group (P<0.05). The double immunofluorescence staining showed the colocalization of GFAP and AQP-4 in the astrocytes. Conclusion MB may ameliorate the BBB disruption induced by focal cerebral ischemia-reperfusion through reducing glio-cyte proliferation and down-regulation of AQP-4 expression in rats.

By retrieving the clinical research literature of treatment functional dyspepsia by traditional Chinese medicine (TCM) from January 2004 to December 2014 based on China National Knowledge Internet (CNKI), we would establish a TCM decoction database for treating functional dyspepsia in this study. One hundred and sixty-four literature were included, involving 159 prescriptions, 377 medicines, in a total of 1 990 herbs. These herbs can be divided into 18 categories according to the effectiveness; and qi-regulating herbs, blood circulation herbs, and antipyretic herbs ranked top three ones according to the frequency of usage of the herbs, whose medicine usage frequency accounted for 51.81%. Usage frequency of 16 herbs was over 30, and Atractylodes, Radix, Poriaranked top three according to the usage frequency. Medicinal properties were divided into 9 kinds according to the frequency statistics, and the top three were warm, flat, and cold. Taste frequency statistics were classifiedinto 9 kinds, and the top three were acrid, sweet, and bitter. In frequency statistics of the meridian tropism of herbs, it was classifiedinto 11 kinds, and the top three were spleen, stomach, lung. The analysis can provide a reference for treatment and study of TCM of functional dyspepsia.
China ; Databases, Bibliographic ; Drugs, Chinese Herbal ; chemistry ; therapeutic use ; Dyspepsia ; drug therapy ; physiopathology ; Humans ; Internet ; Spleen ; physiopathology ; Stomach ; physiopathology

Objective:To systematically evaluate the therapeutic efficacy of tuina therapy for primary insomnia.Methods:Nine Chinese and English databases were searched from the inception to May 2017 to identify randomized controlled trials (RCTs) studying tuina therapy for insomnia.The enrolled articles were all RCTs with tuina as the monotherapy or major therapy in the experiment group,with clear diagnostic criteria for primary insomnia well recognized worldwide or in China,and Pittsburgh sleep quality index (PSQ I) as one of the outcome measures.Two researchers evaluated the risk of bias and quality of the enrolled studies by following Cochrane Handbook version 5.1.0.The meta-analysis was performed by RevMan version 5.3.Results:Eleven studies were included with a total of 1 076 participants.The Western medication adopted in the control groups were benzodiazepine receptor agonists.The studies were all assessed as high risk of bias for blinding since blinding method was unable to be performed due to the specificity of tuina therapy;no study reported the support of fund or potential interest conflict,so they were all rated unclear for selective reporting.The meta-analysis showed that compared with other traditional Chinese medicine therapies,tuina worked more effectively in reducing the PSQI score (MD=-4.11＜0,95％ confidence interval (CI)-6.01 to-2.22,P＜0.0001);compared with oral administration of Western medication,tuina showed more significant efficacy in reducing the PSQI score (MD=-3.42＜0,95％CI-5.19 to-1.66,P＜0.0001).Subgroup analysis showed that head tuina alone showed no significant difference compared with oral administration of Western medication regarding the change of PSQI score (MD=-4.19＜0,95％CI-8.87 to 0.50,P＞0.05);a combination of head and back tuina could more effectively reduce the PSQI score compared with oral administration of Western medication (MD=-2.08＜0,95％CI-3.09 to-1.06,P＜0.0001).Conclusion:Tuina can produce more significant efficacy in treating primary insomnia compared with other traditional Chinese medicine therapies and oral administration of Western medication,especially the combination of head and back tuina.

Objective To explore the effects of problem-based learning (PBL) on improving intern doctors' clinical thinking and problem-solving capacities in clinical practice teaching.Methods PBL teaching courses were designed and used among 72 intern doctors come from 2010 grade clinical medical major (experiment group)and traditional teaching model was applied in 55 intern doctors come from 2009 grade clinical medical major ( control group).The PBL teaching effectiveness was evaluated through expert comment,exam and questionnaire survey.Test was conducted concerning the score of objective theoretical test on clinical thinking and the quality of case report writing.Results There were differences between experiment group and control group in theoretical test score and case report writing quality ( P ＜ 0.01 ).According to the results of questionnaire survey,teachers and students thought that the PBL teaching mode was better than traditional teaching mode in cultivating students' clinical thinking ability and problem solving ability.Conclusion The teaching effect of PBL in clinical practice is satisfactory.PBL teaching can effectively develop the students' clinical thinking and problem-solving capacities and play an active role in improving education quality and promoting education reform.

Objective To investigate the respective effects of highly concentrated amino acids, highly concentrated glucose and mixture of the above two on the proliferation and extracellular matrix synthesis of glomerular mesangial cells. Methods Mesangial cells were cultured with mannitol (13.3 mmol/L ), highly concentrated amino acids (13.3 mmol/L), highly concentrated glucose (30.5 retool/L) and the mixture of highly concentrated amino acids and highly concentrated glucose repectively. Mesangial cell proliferation was examined by MTT and flow cytometry. The mRNA expression of TGF-β1,ColⅣ and fibronectin (FN) was detected by semi- quantitative RT-PCR, and the protein expression was detected by ELISA, Western blot and radioimmunoassay. Results (1) Cellular proliferation was increased in response to amino acids and was further enhanced by increased levels of both amino acids and glucose in a time-depentent manner. And the most prominent action occured at 48 hours. (2) Among the three group, mesangial cell cycle was affected, and the number of G0/G1 mesangial cells was reduced, but the number of cycle S cells was improved. (3) In the three group, the mRNA and protein expression of TGF-β1 was improved [protein unit: ng·ml-1·(105 cells)-1, 3023±85, 3163±80, 3321+85 vs 1506±63, P< 0.05]. (4)In the three group, the mRNA and protein expression of Col Ⅳ and FN was enhanced [protein unit: ng'ml-1(105 cells)-1, Col Ⅳ:16.78±2.56, 18.65±2.56, 22.83±2.45 vs 10.22±2.54, P<0.01; FN:18.47±2.34, 13.58±3.13, 17.98±2.56 vs 8.22±2.54, P<0.05]. The expression of Col Ⅳ mRNA and protein was stronger when the cells were cultured with the mixture of highly concentrated amino acids and highly concentrated glucose. Conclusions Highly concentrated amino acid can promote cell proliferation similarly to highly concentrated glucose, which may be through increasing the activity of mesangial cells and the synthesis of extracellular matrix via TGF- β1 signaling way. Highly concentrated amino acid has the synergism with highly concentrated glucose.

Objective To investigate the relationship between surfactant associated protein-A(SP-A) in bronchoalveolar lavage fluid(BLAF) and lung function in children with refractory mycoplasma pneumoniae pneumonia(RMPP).Methods Thirty children with RMPP were selected from January 2015 to December 2016 in the People's Hospital of Hebi City.The partial pressure of oxygen in artery (PaO2),partial pressure of carbon dioxide in artery (PaCO2),forced expiratory volume in one second(FEV1) and forced vital capacity (FVC) of the children were detected at acute and convalescent periods.The BALF was collected by bronchoalveolar lavage,and the level of SP-A in BALF was detected by enzyme linked immunosorbent assay.Results The level of SP-A in BALF of children with RMPP at ac ute and convalescent period was (3.63 ± 0.09) and (5.86 ± 0.17)mg · L-1 respectively,the level of SP-A in BALF of children with RMPP at acute phase was significantly lower than that at convalescent period(t =-63.499,P ＜ 0.05).The PaO2 and PaCO2 in children with RMPP at acute phase were (49.25 ±7.32) and (47.16 ±6.48)mmHg respectively,and they were (76.54 ±6.48) and (36.20 ± 5.61)mmHg respectively at convalescent period;the PaO2 in children with RMPP at acute phase was significantly lower than that at convalescent period (t =-15.289,P ＜ 0.05),and the PaO2 in children with RMPP at acute phase was significantly higher than that at convalescent period(t =7.004,P ＜ 0.05).The FEV1,FVC and FEV1/FVC in RMPP children at acute phase were (1.36 ±0.67),(1.68 ± 0.31) L and 69.85 ± 8.34 respectively;and they were (1.89 ± 0.58),(1.99 ± 0.53) L and 87.32 ± 9.52 respectively at convalescent period;the FEV1,FVC and FEV1/FVC in RMPP children at acute phase were significantly lower than those at convalescent period(t =-3.276,-2.765,-7.560;P ＜ 0.05).The level of SP-A in BALF of children with RMPP was positively correlated with PaO2 (r =0.921 6,P ＜ 0.05),but there was no significant correlation between SP-A level and PaCO2 (r =1.211 4,P ＜ 0.05).The level of SP-A in BALF was positively correlated with FEV1,FVC and FEV1/FVC (r =0.831,0.905,0.803;P ＜ 0.05).Conclusion The level of SP-A in BALF was positively correlated with lung function of children with RMPP.The detection of SP-A level in BALF is helpful to assess the lung function and pathogenetic condition of children with RMPP.