1.Adopting a people-oriented approach in building the emergency department
Qing ZENG ; Zeqing DI ; Zhinan CHAI
Chinese Journal of Hospital Administration 1996;0(02):-
Using information on the Emergency Department of the Sino-Japanese Friendship Hospital-reconstructed in January of 2002-as primary data, the authors provide a general view of the model for the building, management and development of emergency departments in China in the 21" century. There are two aspects to the issue. One concerns the composition of the hardware architecture of the emergency department, including emergency medical service system(EMSS), modified layout and facilities, medico-technical departments with a complete range of functions, rational emergency medical service procedures as well as pie-hospital first aid and facilities for long-distance network medical treatment. The other concerns the composition of the software architecture of the emergency department, including the transformation of the employment system of emergency medical personnel, the change of emergency service concepts, the personnel performance and financial control of the emergency department, and the development of an emergency department culture.
2.Discussion on Clinical and Diagnosis Program of Integrative Medicine.
Yi-di ZENG ; Ze-biao CAO ; Jia DU ; Jing-jie TAO ; Xiao-qing ZHOU
Chinese Journal of Integrated Traditional and Western Medicine 2016;36(5):517-521
Facing current situation of integrative medicine (IM), authors put forward that clinical and diagnosis program of IM could be carried out from clinical path, pathogenesis, treatment theory and philosophy, and so on, but with different integration degrees. Meanwhile, formulation of concrete program should be disease-targetedly set up, and adjusted from person to person, from place to place, from time to time. As for settled IM program , authors could evaluate it from whether Chinese medicine and Western medicine have formed complementary, synergistic, excitatory actions, and toxicity attenuation; whether more problems could be solved in efficacy, safety, practicability, and economy than previous single mode.
Critical Pathways
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Integrative Medicine
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trends
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Medicine, Chinese Traditional
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trends
3.Effect of EGFP gene transfection on the cell cycle distribution of primary cultured human chondrocytes
Xun JIANG ; Yaoying ZENG ; Xianhui HE ; Lihui XU ; Jingfang DI ; Zheng FENG ; Jingxian ZHAO ; Qing WANG ; Tong WANG ; Jianbo SHI
Chinese Journal of Pathophysiology 1989;0(06):-
AIM: To investigate the effect of enhanced green fluorescence protein (EGFP) gene transfection on the cell cycle distribution of primary cultured human chondrocytes in order to establish a tracking method of cultured human nasoseptal chondrocytes. METHODS: pEGFP-N1 plasmid was amplified in E.coli, and purified by high purity kit. Primary cultured human chondrocytes,which were initially obtained from the nasoseptal cartilage, were cultured in vitro and transferred with pEGFP-N1 by means of electroporation with Amaxa nucleofector device. Transfering process and transient expression were evaluated by laser scanning confocal microscope (LSCM), the transfer efficiency and the cell cycle distribution were evaluated by flow cytometry. RESULTS: There was significant expression of EGFP at 24 h after transferring. The transfection efficiency of pEGFP-N1 into primary cultured human chondrocytes reached 35 37% at 48 h. It didn't affect the process of cell adherance and had no effect on the cell cycle distribution. CONCLUSION: Primary cultured human chondrocytes, which were transfected with pEGFP, are alive in vitro, and the transferring process doesn't affect the cell cycle distribution. These results suggest that pEGFP-N1 is an ideal transient expression vector for primary cultured human chondrocytes and it might be a well tracer in construction tissue engineered cartilage.
4.Reducing effect of curcumin on expressions of TNF-alpha, IL-6 and IL-8 in rats with chronic nonbacterial prostatitis.
Qing-Yun ZHANG ; Zeng-Nan MO ; Xi-Di LIU
National Journal of Andrology 2010;16(1):84-88
OBJECTIVETo investigate the effects of curcumin on the expressions of TNF-alpha, IL-6 and IL-8 in rats with chronic nonbacterial prostatitis.
METHODSSixty healthy adult male SD rats with the body weight of 200 -220 g were equally and randomly divided into a normal control, a positive control, a model, an oral curcumin and an intraperitoneal curcumin group. The rat models of chronic nonbacterial prostatitis were made by hypodermic injection of estradiol benzoate at the dose of 0.25 mg/(kg x d) for 30 days after castration, and then treated with curcumin at 200 mg/(kg x d) by gavage or intraperitoneal injection. The positive controls received oral celebrex at 250 mg/(kg x d), while the normal control and model groups were given saline by gavage. After a week of treatment, the levels of TNF-alpha, IL-6 and IL-8 in the serum and prostate tissues of the rats were detected by ELISA assay.
RESULTSThe levels of TNF-alpha and IL-8 in the serum and prostate tissues were significantly lower in the intraperitoneal curcumin than in the positive control group (P < 0.05), but the expression of IL-6 showed no significant difference between the two groups (P > 0.01).
CONCLUSIONCurcumin is efficacious for chronic nonbacterial prostatitis in rats, and the action mechanism may be associated with its decreasing effect on the proinflammatory cytokines IL-8 and TNF-alpha in the blood and tissues.
Animals ; Chronic Disease ; Curcumin ; pharmacology ; therapeutic use ; Drugs, Chinese Herbal ; therapeutic use ; Interleukin-6 ; metabolism ; Interleukin-8 ; metabolism ; Male ; Phytotherapy ; Prostatitis ; drug therapy ; metabolism ; Rats ; Rats, Sprague-Dawley ; Tumor Necrosis Factor-alpha ; metabolism
5.The role of ADME evaluation in translation research of innovative drug.
Yao LIU ; Lan HONG ; Lu-Shan YU ; Hui-Di JIANG ; Jian-Zhong CHEN ; Qin MENG ; Shu-Qing CHEN ; Su ZENG
Acta Pharmaceutica Sinica 2011;46(1):19-29
New Chemical Entities (NCEs) development is a systematic long-term project that involves multiple disciplines. The translation research will help to build an advanced R&D system from the basic laboratory research, preclinical studies and clinical evaluation to clinical application of drug, for the purpose of shortening the R&D cycle and accelerate the launch of new drugs. In new drug R&D and its clinical application, drug disposition (absorption, distribution, metabolism, excretion, ADME) properties are important criteria for assessing drug-likeness of candidates. ADME evaluation of NCEs plays an important role in the translation research throughout innovative drug R&D process. Therefore, ADME evaluation at the early stage of drug design and development will be helpful to improve the success rate and reduce costs, and further access to safe, effective drugs.
Absorption
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Biological Transport
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Drug Design
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Drug Evaluation, Preclinical
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Pharmaceutical Preparations
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chemistry
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metabolism
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Pharmacokinetics
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Tissue Distribution
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Translational Medical Research
6.Study on the Fingerprint of Kingkong Zedoary Turmeric Oil.
Shu-ling WANG ; Tian XIE ; Zhao-wu ZENG ; Xiao-qing PANG ; Nan LIU ; You-jian YANG ; Hong ZHAO ; Di-fei ZHANG
Chinese Journal of Integrated Traditional and Western Medicine 2016;36(6):744-748
OBJECTIVETo study the fingerprint of Zedoary Turmeric Oil (ZTO) as the bulk drug of Kingkong Elemene for making it safe, effective, stable, and controllable.
METHODSFingerprints were detected by gas chromatography. β-elemene peak was regarded as reference peak (S). The relative peak area of each common peak and the relative retention time were calculated. With a total of modes for reference, the fingerprints of 10 batches of Kingkong ZTO were detected, and their similarity was calculated by traditional Chinese medicine (TCM) fingerprint similarity calculation software.
RESULTSThe determination method was stable and reliable. Totally 19 common characteristic peaks of Kingkong ZTO was found. The fingerprint similarity of these batches of Kingkong ZTO were not lower than 0.96.
CONCLUSIONSGas chromatography for detecting the fingerprint of Kingkong ZTO was reliable and repeatable. The established fingerprint of Kingkong ZTO could guarantee the quality stability and safety of different product batches.
Chromatography, Gas ; Curcuma ; chemistry ; Drugs, Chinese Herbal ; chemistry ; Plant Oils ; chemistry ; Sesquiterpenes ; chemistry
7.Efficacy and Safety of TacroUmus Ointment for the Treatment of Atopic Dermatitis in Chinese Children
Lingling LIU ; Xia DOU ; Zhiqimg XIE ; Di WANG ; Zhizhong ZHENG ; Minghui WEI ; Bie YU ; Baoxi WANG ; Donglai MA ; Lin LIN ; Juanqin GONG ; Fanqin ZENG ; Qing GUO ; Xuejun ZHU
Chinese Journal of Dermatology 2003;0(10):-
Objective To evaluate the efficacy and safety of 0.03% tacrolimus ointment for the treatment of atopic dermatitis (AD) in Chinese children. Methods A total of 139 children, 2 to17 years of age, with moderate to severe AD from 5 study centres were enrolled in this multicentre, randomized, double-blind, vehicle-controlled, parallel group study. Treatment with 0.03% tacrolimus ointment or vehicle was applied twice daily to the affected areas for 3 weeks. Visits were scheduled on day 1 (base line, before treatment) and 1, 2, 3 weeks after the treatment. The main therapeutic parameter was the efficacy rate at the end of the treatment. Results The efficacy rates were 84.6% and 29.0% for tacrolimus group and vehicle group, respectively (P
8.An analysis on the level of homocysteine and related factors among healthy examination population
Ting-Ting YU ; Wang-Di LU ; Qing-Yang SUN ; Ying-Qiang ZHANG ; Xian-Ming ZENG ; Jun XIA
Journal of Preventive Medicine 2017;29(3):248-250
Objective To investigate the level of homocysteine among healthy examination people and the possible related factors.Methods Retrospective analysis was performed to collect 1 259 results of healthy examination people from July to September in 2015,and 564 patients were confirmed to be hyperhomocysteinemia.The serum level of lipoids,glucose,uric acid and blood routine results were also collected.Results The incidence of hyperhomocysteinemia was 45.52%,and man has a higher rate than woman.The results of logistic regression showed positive results of UA (OR =1.006,95% CI =1.005-1.008),HBG(OR =1.035,95%CI=1.026-1.045),and PLT (OR =0.996,95% CI =0.993-0.998) in high hyperhomocysteinemia patients.Condusion High UA、HBG and low PLT levels are risk factors in hyperhomocysteinemia,and could be the important way for the early diagnoses of hyperhomocysteinemia.
10.Effects of Gardenia-aweto compound by different extraction method on antagonizing acute respiratory distress syndrome.
Dong YAN ; Lan-juan LI ; Wei-bo DU ; Guo-ping REN ; Hong-cui CAO ; Di-feng ZHU ; Lei XIA ; Zhen ZENG ; Qing SHAO ; Hai-jiang ZHANG ; Yi-yu CHEN
China Journal of Chinese Materia Medica 2005;30(9):700-703
OBJECTIVETo study the effect of Gardenia-Aweto compound (GAC) and two component on preventing acute respiratory distress syndrome (ARDS) by the rabbit model of ARDS induced by intravenous injection of oleic acid. To detect the efficiency component of GAC in preventing ARDS.
METHODGAC was divided into two compounts, ethanol-soluble components (ESC) and ethanol-deposition components (EDC), based on polarity. Forty-three new zealand rabbits were randomly divided into five groups, the blank control group, the model group, the GAC groups, the ESC group, and the EDC group. The ARDS model was induced by intravenous injection of oleic acid. Dynamic changes of arterial blood gas, lung index, albumin in bronchoalveolar lavage fluid (BALF) in different groups and lung histological changes were observed and compared.
RESULTAs compared with the blank group, in the model group, GAC group, ESC group, EDC group the arterial PO2 and oxygen saturation deprived continuously. While SO2 in GAC group at time points 30, 60, 90, 120 min (P < 0.05 or 0.01) and SO2 in ESC group at time points 30, 60, 90 min were higher than those in ARDS group. PO2 in ESC group at time points 30, 60 min (P < 0.05) were higher than those in ARDS group. The value of LI and W/D were higher in ARDS group than in sham group (P < 0.01), they were much lower in HD group than in ARDS group (P < 0.01). Concentration of BALF-albumin increased markedly in ARDS group and pre-treatment groups compared with sham group, but it was much lower in GAC group and ESC group, there was a significant difference between GAC group (P < 0.01), ESC group (P < 0.05) and ARDS group. The lung histological changes had been improved in GAC group and ESC group. But no significantly difference between above-mentioned parameters was found in comparison in the model group and in the EDC group.
CONCLUSIONPreventive administration of GAC or ESC an protect the damaged lung function in ARDS rabbits induced by oleic acid. The efficiency component of GAC in preventing ARDS is ESC. GAC antagonizing ARDS may relate to its anti-inflammatory, immuno-modulatory, anti-oxidant and antithrombotic effects.
Animals ; Cordyceps ; chemistry ; Drug Combinations ; Drugs, Chinese Herbal ; isolation & purification ; therapeutic use ; Female ; Gardenia ; chemistry ; Lepidoptera ; Male ; Materia Medica ; isolation & purification ; therapeutic use ; Oleic Acid ; Phytotherapy ; Pulmonary Gas Exchange ; Rabbits ; Random Allocation ; Respiratory Distress Syndrome, Adult ; chemically induced ; pathology ; prevention & control