1.STUDIES ON BLEACHING OF PULP BY WHITE-ROT FUNGUS
De-Qing ZHAO ; Lu LIN ; Li-Ping JIANG ;
Microbiology 1992;0(06):-
This article summarizes the bleaching of pulp by white-rot fungus, including the type of white-rot fungus, the type of pulp, the equipment used to bleach by white-rot fungus, and some problems and prospect in practical application by white-rot fungus.
2.A Study on the Effects of RF Coil for the Static Magnetic Field
Hong WANG ; Qing-De LIN ; Feng HUO
Chinese Journal of Medical Instrumentation 2001;25(3):139-140
In the paper, we introduce the studies on some abnormal points in static magnetic field,find out the causes and mean while, give the methods of exclusion, thus the adjustment of MRI can be made perfectly.
3.Analysis of monitoring results on plague's host animal and its medium insects in Quanzhou city of Fujian province between 2000 and 2009
Feng-ping, LI ; Wei-ming, WANG ; De-lin, YANG ; Qing-hu, ZHANG
Chinese Journal of Endemiology 2011;30(5):518-520
Objective To examine the composition and distribution of animal reservoir of plague in Quanzhou city, Fujian province, and so as to accumulate basic data for making the plague prevention and control measures. Methods Rodents were captured by cages in six monitoring spots of Quanzhou city between 2000 and 2009. Then rodents and flea species were identified. Rats liver and spleen homogenates were detected of plague F1 antigen by reverse indirect hemagglutination test (RIHA); rats serum were detected of plague F1 antibody by indirect hemagglutination test (IHA). ResultsA total of 26 264 rodents were captured between 2000 and 2009.Rattus norvegicus and Rattus flavipectus were dominant species, which were accounted for 45.97% (12 074/26 264)and 32.01%(8407/26 264), respectively. The account of Rattus flavipectus captured between 2005 and 2009 was (26.99 ± 2.46)% and (37.03 ± 3.79)% between 2000 and 2004. The difference was statistically significant (t =4.97, P < 0.05). Total rodent densities was (6.86 ± 1.44)%, including(5.36 ± 1.83)% in mountains and (6.81 ±1.66)% in coastal areas, respectively, and the difference was not statistically significant(t =1.01, P > 0.05). Total flea index and flea infection rate of rodents were 1.39 ± 0.34 and (35.90 ± 5.34)%, respectively. Xenopsylla Cheopis index was 1.20 and free flea index was 0.009. Flea infection rate was (32.36 ± 0.96)% between 2005 and 2009, which was lower than (39.44 ± 0.39)% between 2000 and 2004(t =2.76, P < 0.05). ConclusionsThe major rodent species found in Quanzhou city is Rattus norvegicus. Xenopsylla Cheopis is the dominant flea species.Spreading of plague among Rattus is not found.
4.An improved method of quantitative assessment of regional cerebral blood flows by perfusion CT at the general infusion rate
Chun-Hong HU ; Qing-De WU ; Xue-Yuan WANG ; Wei ZHU ; Hai-Lin SHEN ; Yin-Di FU ; Yi DING ;
Chinese Journal of Radiology 2001;0(01):-
Objective To improve the conventional method of quantitative assessment of regional cerebral blood flows(rCBF)by a perfusion CT study based on maximal slope model at the general infusion rate(
5.Effects and Safety of Hemoperfusion Therapy on Infants with Tetramine Poisoning
de-xuan, WANG ; rui-xia, LIN ; qing, YANG ; guo-quan, PAN ; chun-xue, YAN ; ling-wei, JIN
Journal of Applied Clinical Pediatrics 1992;0(06):-
Objective To explore the effects and safety of hemoperfusion(HP) therapy on tetramine poisoning in infants.Methods Thirty-five infants with tetramine poisoning were divided into two groups: HP group(n=18) and non HP group(n=17).The changes of blood tetramine concentration and clinical symptom improving of both groups after the treatment were observed together with the adverse effects of HP group.Results The average blood tetramine concentration of HP group was higher than that of non HP group(342.2?333.4 vs 117.9?50.8 ?g/L,P
6.Preliminary study on rehydrated conditions for lyophilized human red blood cells.
Lin-Feng CHEN ; Jing-Han LIU ; De-Qing WANG ; Xi-Lin OUYANG ; Yuan ZHUANG ; Ji CHE ; Hui LI
Journal of Experimental Hematology 2009;17(6):1582-1587
The objective of this study was to investigate the effect of different rehydration conditions on recovery of the lyophilized red blood cells (RBC) so as to optimize the RBC rehydration. The different conditions, including different rehydration solution, the rehydration temperature, volume change rate of the lyophilized RBC rehydrated by the vapor firstly, were studied, the recovery rate and change of physiological and biochemical properties of the rehydrated RBC were detected. The results indicated that the solution of 10% (w/v) PVP40 in PBS showed the best effect, and the RBC recovery rate increased with increasing of rehydration temperature, and the optimal temperature of rehydration was at 37 degrees C. Pre-rehydration in condition of vapor could raise the RBC recovery rate, and promote the MCV and RDW to close to index of the fresh RBC, the deformability of the rehydrated RBC was no serious as compared with RBC preserved in conventional condition, but the activity level of ATP, G-6-PD, SOD, 2, 3-DPG of the rehydrated RBC less decreased. It is concluded that the optimal rehydration conditions for lyophilized RBC are pre-rehydration in the 37 degrees C with vapor firstly, PBS + 10% (w/v) PVP40 rehydration solution and rehydration temperature at 37 degrees C, but the protection of RBC membrane needs to be furtherly studied.
Blood Preservation
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7.The relationship between the peripheral blood of CD61, CD63, PAC-1 and the transplant kidney function.
Yong ZHANG ; De-lin GUAN ; Cheng-qing XIA ; Zhi-you HAN ; Jian-jun XU ; Ju-zhong GAO ; Ke-rang WU
Chinese Journal of Surgery 2003;41(12):881-884
OBJECTIVESTo explore the relationships between the peripheral blood levels of CD61, CD63, PAC-1 and the incidence of acute rejection and tubular necrosis after renal transplantation, and recovery of the graft function.
METHODSThe peripheral blood levels of CD61, CD63, and PAC-1 of 86 patients with uremia in different stages before and after transplantations were analyzed by flow cytometry. The patients were divided into three groups: (1) twenty-nine patients with normal grafts function, (2) hirty with acute rejection and (3) twenty-seven with acute tubular necrosis. The patients with acute rejection were randomly divided into treatment group with anticoagulants and cntrol group.
RESULTSThe peripheral blood levels of CD61, CD63 and PAC-1 significantly increased (P < 0.05) in the patients with acute rejection, in comparison with those with normal grafts function and those with acute tubular necrosis. The peripheral blood levels of CD61, CD63 and PAC-1 in patients with acute rejection in anticoagulants therapy was lower, recovery time of the grafts function was shorter, one-year survival rates of patients and grafts were higher, as compared with those of controls.
CONCLUSIONSThe patients with acute rejection have significantly high peripheral blood levels of CD61, CD63 and PAC-1 before transplantation, however, these values in patients with acute tubular necrosis are not high, this suggesting that acute rejection might relate to platelet activation, while acute tubular necrosis might not relate to it. After anticoagulants therapy in patients with acute rejection, the grafts function might recover faster and their one-year survival rates and grafts might be higher in those with CD61, CD63 and PAC-1 decreasing remarkably.
Adult ; Aged ; Antigens, CD ; blood ; Dual Specificity Phosphatase 2 ; Female ; Graft Rejection ; Humans ; Integrin beta3 ; blood ; Kidney ; physiopathology ; Kidney Transplantation ; Male ; Middle Aged ; Platelet Activation ; Platelet Membrane Glycoproteins ; Protein Phosphatase 2 ; Protein Tyrosine Phosphatases ; blood ; Tetraspanin 30
8.Mechanism of Ca2+ on the hyperthermia-induced apoptosis of rat hippocampal neurons in vitro.
Guang-zhong CHEN ; Bing-de LUO ; Xian-hong CHEN ; Qing-ping ZHAO ; Fei ZOU ; Tie-lin LI
Chinese Journal of Industrial Hygiene and Occupational Diseases 2005;23(3):185-187
OBJECTIVETo study the mechanism of Ca(2+) on the apoptosis induced by hyperthermia in neonate rat hippocampal neurons to provide the applicative evidence of dantrolene for preventing brain injuries.
METHODSDantrolene, Ca(2+) specific blocking agent, was used in the hyperthermia-induced apoptosis of primary hippocampal neurons in vitro to observe its effect on the apoptosis, fluorescent intensity, and dynamic change of Ca(2+) by flowcytometry and laser confocal microscopy.
RESULTSThe rate of apoptosis was decreased significantly after hyperthermia treatment by dantrolene sodium. The intracellular Ca(2+) fluorescent intensity in 42 degrees C treatment group (107.35 +/- 6.0) was significantly lower than that in control group (159.12 +/- 33.8). The concentration of Ca(2+) began to decrease 20 approximately 25 s after adding dantrolene sodium, and reached the lowest level about 50 s later, and then kept lower than the basal level.
CONCLUSIONDantrolene sodium has an important protective effect on hippocampal neurons apoptosis induced by hyperthermia and may have some applicative value of preventing heat-induced brain injury.
Animals ; Animals, Newborn ; Apoptosis ; drug effects ; Calcium ; metabolism ; Calcium Channel Blockers ; pharmacology ; Cells, Cultured ; Dantrolene ; pharmacology ; Female ; Hippocampus ; cytology ; drug effects ; Male ; Neurons ; drug effects ; metabolism ; Rats ; Rats, Sprague-Dawley ; Temperature
9.Arterial blood gas analysis in Lipopolysaccharide-heat co-stressed rats.
Xiao-jing LIN ; Bing-de LUO ; Ya-jie LI ; Zhi-rong ZHAO ; Qing TAN ; Bin WANG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2006;24(3):164-166
OBJECTIVETo observe the change in vital signs and arterial blood gas in Lipopolysaccharide (LPS)-injected heat exposed rats.
METHODSMale pathogen-free Wistar rats were randomly assigned to the following groups: saline-injected normothermic control (C-Group), saline-injected heat exposed (H-Group), LPS-injected normothermic control (L-Group), LPS-injected heat exposed (HL-Group). Rectal temperature (Tr), heart rate (HR), mean arterial pressure (MAP), arterial blood gas were continually monitored.
RESULTS(1) The rats in HL-Group displayed significantly high values of Tr (43.04 degrees C +/- 0.11 degrees C) and HR [(660 +/- 42) beats/min] and low values of MAP [(49.0 +/- 3.5) mm Hg] compared with C-Group. There was a significant difference in the values of Tr, HR, and MAP between HL-Group and L-Group and in the values of HR and MAP between HL-Group and H-Group. (2) The values of PaO(2), HCO(3)(-), PaCO(2) were significantly lower than those in C-Group at 40 min after LPS-injected heat stress. At 120 min, the PaO(2) [(11.59 +/- 1.11) kPa], HCO(3)(-) [(10.42 +/- 1.06) mmol/L], PaCO(2) [(2.82 +/- 0.81) kPa] in HL-Group were significantly lower than those in L-Group. A significant difference in the values of HCO(3)(-) and PaCO(2) between HL-Group and H-Group was also observed.
CONCLUSIONLPS-injected heat stress primes the rat to advance and augment the change in vital signs, arterial blood gas, and systemic inflammatory response syndrome.
Animals ; Blood Gas Analysis ; Blood Pressure ; physiology ; Body Temperature ; physiology ; Heart Rate ; physiology ; Heat Stress Disorders ; blood ; physiopathology ; Lipopolysaccharides ; toxicity ; Male ; Random Allocation ; Rats ; Rats, Wistar
10.Functional and structural recovery of injured spinal cord following delayed X-irradiation in rats.
Gang LI ; Xin-Gang LI ; De-Ze JIA ; Dong-Hai WANG ; Yu-Hang SU ; Qing-Lin ZHANG
Chinese Medical Sciences Journal 2007;22(1):58-61
OBJECTIVETo test the hypothesis that delayed X-irradiation can enhance the functional and structural recovery of the injured spinal cord in rats.
METHODSSeventy Sprague-Dawley rats were randomly divided into two groups, 35 rats in each. The control group sustained a one-minute clip compression (force of clip was 30 g) injury of the spinal cord at the T2 level, without X-irradiation. The experimental group received X-irradiation 14 days after injury. Neurological function was assessed by the modified Tarlov method, including hind limbs movement, inclined plane, and pain withdrawal. These tests were performed in a blinded fashion at 3, 7, 14, 21, 28, 35, and 42 days after injury. At 43 days after injury, histological examination of the injured spinal cord was performed following decapitation of the rats.
RESULTSSixty-two rats met the experimental requirements (spinal cord injury was similar), 32 rats in experimental group and 30 rats in control group. Statistically significant difference was observed between the two groups in hind limbs movement and inclined plane (P < 0.01), but not in the pain withdrawal test. The edema and necrosis areas of injured spinal cords in experimental group were less than those in control group, and axons in experimental group were significantly more than those in control group (P < 0.01).
CONCLUSIONDelayed X-irradiation following spinal cord injury may enhance functional recovery by improving and restoring structural integrity of the injured spinal cord in rats.
Animals ; Axons ; physiology ; radiation effects ; Hindlimb ; Joints ; physiology ; Motor Activity ; Movement ; Radiotherapy ; methods ; Rats ; Rats, Sprague-Dawley ; Spinal Cord ; physiopathology ; radiation effects ; Spinal Cord Injuries ; radiotherapy ; rehabilitation ; Weight-Bearing ; X-Rays