Objective To explore the effect of Angiotensin Ⅱreceptor inhibitor on proliferation and apoptosis and cell cycle of human en-dometrial cancer cell line induced by estrogen.Methods Human endometrial cancer cells,ishikawa were cultured,and then treated with different concentrations of estrogen or saralasin.Cell proliferation was examined using MTT,and cell cycle and apoptosis were determined us-ing flow cytometry.Results Saralsin inhibited the proliferation of ishikawa cells.After treated with saralasin at 10 min after estrogen induc-tion,cycle was arrested at G1/G0 phase and S phase was reduced in ishikawa cell with significant difference between treatment group and control group.An increase in early apoptosis and late apoptosis occurred at 10min after treatment.Conclusion Saralsin inhibits endometrial cancer cell ishikawa proliferation,induces apoptosis and accumulation in G1/G0 phase in vitro.Therefore,saralsin may be helpful for the treat-ment of endometrial cancer.

The clinical supervision of counseling and psychotherapy have a closely connection with clinical employment.Leading the way in clinical supervision,many clinical supervision models has been extrapolated by theories of various psychotherapy,relating to psychoanalysis theory,person-centered approach theory,cognitive and behavior psychotherapy theory,system theory,structuralism theory,and so on.Recently,especial,different clinical supervision models have to expand beyond psychotherapy,such as developmental model of supervision and society role model of supervision.The study has summarized clinical supervision models of the field,in order to offer literature index and concept frame.

Objective To apply laparoscopy-assisted small incision surgery in complicated urological operation, and to summarize our experience. Methods We retrospectively analyzed the clinical data of 9 patients with renal tumors (3 with solitary kidney tumors, 2 with unilateral multiple tumors, 1 with bilateral tumors, and 3 with contralateral renal insufficiency), 3 patients with renal pelvic ureteropelvic junction obstruction (UPJO) and multiple stones, and 1 patient with distal ureteral tumor. All patients underwent laparoscopy-assisted small incision surgery in our department from Aug. 2007 to Jun. 2011. The key steps of the surgery were discussed and the clinical experience was summarized. Results (1) For 9 patients with renal tumors, the incision was 4-6 cm in length, the cold ischemia timewas (15 ±4) min, and the operation time ranged from 90 to 180 min. Drainage tubes were removed within 48 h after operation. No patient had bleeding, urinary fistula or other serious complications. (2) For the 3 patients with UPJO, the incision was 4-5 cm in length and the operation time was 110 to 190 min. Drainage tubes were removed within 3-5 d after operation. No patient had bleeding, urinary fistula or other serious complications. No patient had fever or back pain after removal of double-J stents. Intravenous pyelography showed no hydronephrosis and the renal structure was normal. (3) For the patient with ureteral tumor, the drainage tube was removed 3 d after operation and there was no complication. No patients in the present study used analgesic pump or analgesic medications after surgery. Conclusion Laparoscopy-assisted small incision surgery can reduce the length of incision, minimize injury, and protect renal function. Besides, the method is safe and capable of handling complicated situations, especially suitable for patients who are difficult to receive total laparoscopic surgery.

To apply laparoscopy-asiisted small incision surgery in complicated urological operation, and to summarize our experience. Methods We retrospectively analyzed the clinical data of 9 patients with renal tumors (3 with solitary kidney tumors, 2 with unilateral multiple tumors, 1 with bilateral tumors, and 3 with contralateral renal insuffieiency), 3 patients with renal pelvic ureteropelvic junction obstruction (UPJO) and multiple stones, and 1 patient with distal ureteral tumor. All patients underwent laparoscopy-assisted small incision surgery in our department from Aug. 2007 to Jun. 2011. The key steps of the surgery were discussed and the clinical experience was summarized. Results (1) For 9 patients with renal tumors, the incision was 4-6 cm in length, the cold ischemia time was (15±4) min, and the operation time ranged from 90 to 180 min. Drainage tubes were removed within 48 h after operation. No patient had bleeding, urinary fistula or other serious complications. (2) For the 3 patients with UPJO, the incision was 4-5 cm in length and the operation time was 110 to 190 min. Drainage tubes were removed within 3-5 d after operation. No patient had bleeding, urinary fistula or other serious complications. No patient had fever or back pain after removal of doubie-J stents. Intravenous pyelography showed no hydronephrosis and the renal structure was normal. (3) For the patient with ureteral tumor, the drainage tube was removed 3 d after operation and there was no complication. No patients in the present study used analgesic pump or analgesic medications after surgery. Conclusion Laparoscopy-assisted small ineieion surgery can reduce the length of incision, minimize injury, and protect renal function. Besides, the method is safe and capable of handling complicated situations, especially suitable for patients who are difficult to receive total laparoscopic surgery.

OBJECTIVE: To establish the national reference standards of sulfadimidine impurities thus to provide guarantee for improving the standard of quality control of sulfadiazine in China. METHODS: First, the structures of sulfadimidine impurities A and E were validated by infrared spectrocopy, mass spectrum and nuclear magnetic resonance method. Then, the purities of impurities A and E were determined using the method of related substance test for sulphadiazine in the European Pharmacopoeia (version 9.0),their water content and residue on ignition were determined as well. The contents of sulfadimidine impurities A and E were determined by using mass balance method. Meanwhile, external standard method and nuclear magnetic quantitative method were used to calculate the content, which were mutually verified with the mass balance method. Finally, the correction factors of sulfadiazine impurities A and E at 241 nm were determined using standard curve method. RESULTS: The structures of sulfadimidine impurities A and E were confirmed, and the contents of impurities A and E were 99.1% and 98.7%, respectively, which were calculated by mass balance method. The results were consistent with those obtained from external standard method and nuclear magnetic quantification method. The correction factors of impurities A and E to sulfadimidine were 0.97 and 0.63, respectively. CONCLUSION: The first batch of national standard substances of sulfadimidine impurities A and E were established successfully.

Objective : To investigate the prevalence of electronic cigarette use among adult residents at ages of 15 years and older in Tongzhou District, Beijing Municipality, so as to provide insights into formulation of tobacco control interventions. Methods: Permanent residents at ages of 15 years and older were sampled using a stratified multi-stage cluster random sampling method in Tongzhou District from September to October, 2021, and subjects' demographics, electronic cigarette use, and exposure to electronic cigarette advertising were investigated using the 2021 Questionnaire for Adult Tobacco Survey in Beijing. The awareness of electronic cigarette knowledge, prevalence of electronic cigarette use, route of acquiring electronic cigarette knowledge and route of exposure to electronic cigarette advertising were analyzed among residents in Tongzhou District, and factors affecting electronic cigarette uses were identified using a multivariable logistic regression model. Results: A total of 1 196 questionnaires were allocated, and 1 166 valid questionnaires were recovered, with an effective recovery rate of 97.49%. The respondents included 532 men (45.63%) and 634 women (54.37%), with a mean age of (57.05±15.04) years. The awareness of electronic cigarette knowledge was 67.58%, and the prevalence of electronic cigarette uses and current electronic cigarette uses was 3.94% and 0.34%, respectively. Multivariable logistic regression analysis showed a higher possibility of electronic cigarette uses among men (OR=28.492, 95%CI: 6.873-118.106), residents at ages of 15 to 44 years (OR=3.219, 95%CI: 1.584-6.539), enterprise workers/businessmen/service industry workers (OR=3.599, 95%CI: 1.527-8.482), and public institution workers/public servants/students (OR=4.403, 95%CI: 1.758-11.026). The main route of acquiring electronic cigarettes was presented by others (43.48%), and the main cause of electronic cigarette use was quitting smoking (30.43%), followed by thinking little harmlessness of electronic cigarette use (26.09%), while the main route of exposure to electronic cigarette advertising was television (59.09%), followed by internet (45.45%). Conclusions There is a low prevalence rate of electronic cigarette uses among residents at ages of 15 years and older in Tongzhou District, and men and residents at ages of 15 to 44 years are main electronic cigarette users. Health education pertaining to the harmlessness of electronic cigarette uses and supervision of electronic cigarette advertising are required to be reinforced.

Papers on mycorrhizas were searched from SCI (Science Citation Index) database. In order to understand the research progress of mycorrhizas in China, the paper on mycorrhizas from 1989 to 2007 was analyzed with method of bibliometrics. Mycorrhizal researches showed an increasing tendency during 1989 to 2007, while the increase was obviously accelerated after 2000. Only 5.22% of the articles were published in journals with impact factor above 5, indicates that the academic levels of researches need being enhanced. Currently the research on mycorrhizas was mainly focused on Arbuscular mycorrhiza (AM), especially on effects of mycorrhizal fungi on plant physiology, effects of mycorrhizal fungi on resistance of host plants, Mycorrhizal diversity community and ecological distribution, and phytoremediation with Mycorrhizal plants. The future research should emphasize in phytoremediation with Mycorrhizal plants and identification of fungal species with molecular biological technology.

BACKGROUND: High blood lipid can cause a series of cardiovascular and cerebrovascular disease, which has been commonly accepted.OBJECTIVE: To probe into the rule of changes of blood lipid of the elders in aging process.DESIGN: Cross section survey.SETTING: Department of Nutrition, Shanghai Huadong Hospital. PARTICIPANTS: Totally 605 employees who took part in body examination and participated this experiment voluntarily from July 2000 to August 2004, were recruited, including 541 males and 64 females, 91 patients ＜ 70 years , 514 ≥ 70 years, 271 patients having the history of coronary heart disease, 83 of malignant tumors, and 251 of other diseases.METHODS: Blood lipid level of totally 605 who accepted physical examination was detected, at the same times, the disease history and common physical examination status were inquired. Venous blood of the patients was drawn after fasting over 12 hours, separating serum on the morning of the same day. Total cholesterol and triacylglycerol of the blood were detected with enzyme assay by using Automated Biochemistry Analyzer. Total cholesterol: 3.6-6.5 mmol/L; triglyceride: 0.4-1.8 mmol/L were considered as normal level, Total cholesterol: ＞ 5.72 mmol/L;Triglyceride: ＞1.72 mmol/L were considered as abnormal criteria.MAIN OUTCOME MEASURES: Comparison of the blood lipid index of the subjects of different age, disease type and gender RESULTS: Totally 605 elders were involved and all data of them was enthe blood lipid index of the subjects of different age: Peak of total cholesterol appeared at 70 to 74 years, then decreases gradually, the total cholesterol level of the subjects of 70 to 74 years was significantly higher than that at other age (P ＜ 0.05). While Triglyceride level had the trend of going up with growing older, except that of 75-79 ages group slightly went eases: The level of total cholesterol and triglyceride of the subjects in the malignant tumor group was lower than that of other disease groups, level of triglyceride of the subjects in the coronary heart disease was higher than detection rate of blood lipid of the subjects: Detection rate of total cholesterol was 15.5%, while that of triglyceride was 35.9%. There was no significant difference of the detection rate between male and female subjects (P ＞ 0.05).CONCLUSION: Change of blood lipid metabolism of elder with aging is related to the hormone change of different genders and disease types.

[Objective]To observe the effect of treatment on post-stroke hiccup by acupuncture and medicine.[Methods]58 cases were divided into 2 groups randomly,30 cases were taken the acupuncture and medicine treatment, 28 cases were injected metoclopramide as control. [Results]The curative effect in therapy group is much better than that of control group(P<0.05).[Conclusion]The therapy of acupuncture and medicine is superior to pure medicine in the treatment of post-stroke hiccup.

Objective To evaluate the value of serum tumor markers in diagnosis of over 80 years old patients with colorectal cancer.Methods Serum CA199,CA242 and CEA of 68 cases senile patients with colorectal cancer and 35 cases senile patients with benign large intestinal diseases were detected by ELISA.Results Serum levels of CA199,CA242 and CEA in patients with colorectal cancer were (37.95 ± 15.95 ) U/ml,( 50.08 ± 24.20 ) U/ml,( 22.34 ± 17.35 ) μg/L respectively and were significantly higher than that in patients with benign large intestinal diseases CA199 ( 10.73 ± 6.27 ) U/ml,CA242 ( 8.00 ± 3.52 ) U/ml,CEA(2.32 ± 1.01 )μg/L.In addition,the level of these three markers was increased with the progress of colorectal cancer and the sensitivity of diagnosis was related with the stages of colorectal cancer.Parallel combined testing methods increased the diagnostic sensitivity to 83.6％,and serial combined testing increased the diagnostic specificity to 88.7 ％.Conclusions Serum tumor markers showed certain values in the diagnosis of the senile patients with colorectal carcinoma.Furthermore,the combined determination of tumor markers is helpful for early diagnosis of colorectal cancer and increases the sensitivity and specificity in diagnosing of colorectal carcinoma.