Charged particle radiation is one of the most precise approaches for external radiation right now. With a characteristic inverted depth?dose profile and a high?dose Bragg peak at the end of the range, charged particle radiation therapy can deliver a high dose of radiation to tumor without damages in tissue around the tumor. However, a shift of Bragg peak would result in severe consequences. Therefore, it is extremely important to monitor the radiation beam. Application of position emission tomography ( PET) in proton and heavy?ion radiation therapy makes it possible to get accurate radiation range and position of the particle beam, which improves the treatment accuracy of charged particle therapy. This paper introduces the current application of PET in position verification of charge particle range radiation therapy.

Objective To explore the bio-effects of high single-dose irradiation on MDA-MB-231 xenografts under the same radiation dose.Method Female BALB/c-nu mice bearing 8-9 mm MDA-MB-231 xenografts were randomized into 5 groups:0Gy group (blank control group),high single-dose 8Gy group (8Gy/one fraction/day),high single-dose 10Gy group (10Gy/one fraction/ day),conventional radiation 2Gy × 4 group (8Gy/4 fraction/4d),conventional radiation 2Gy × 5 group (10Gy/5 fraction/5d).The volumes of gross tumors on nude mice were observed every three days.The tumor growth curve of transplanted tumor were also drawn.The 18F -FDG PET/CT imaging of mice bearing MDA-MB-231 xenografts from all radiation groups were performed with detecting the Ki-67 expressions of tumor ceils by immunohistochemistry at 7days and 14days after final irradiation.Results Compared to control group,the tumor growth of all radiation groups were delayed after radiation,especially single high-dose 10Gy groups (P ＜ 0.05).At 7days and 14days after final radiation,the SUVmax (the maximal standardized uptake value) of xenografts in high single-dose groups was lower than that in conventional fractional groups (P ＜ 0.05) under the same radiation dose.And the percent of Ki-67 positive cells after high single-dose irradiation were less than fractioned irradiation at 7,14days after final radiation (P ＜ 0.05).Conclusion At the same dose,high single-dose irradiation inhibits the growth of MDA-MB-231 xenografts more than conventional fraction irradiation.PET/CT also could evaluated the early changes of proliferation activities of MDA-MB-231 breast cancer cells after irradiation.

Objective To compare the results difference between the fluorescence staining and the acid fast staining (Ziehi‐Neelsen staining) methods in the detection of Mycobacterium tuberculosis ,and to compare the effects of the methylene blue solu‐tion ,Haris hematoxylin solution and potassium permanganate liquid as the redyeing reagents of the fluorescence staining method . Methods 198 sputum specimens collected from the patients with suspected tuberculosis symptoms and were performed the Ziehi‐Neelsen staining and the fluorescence staining respectively For comparing the difference in the detecting rate of Mycobacterium tu‐berculosis between the two kinds of method .The fluorescence staining adopted 0 .3% methylene blue solution ,0 .5% Haris hema‐toxylin solution and 0 .5% potassium permanganate solution as the redyeing reagents for comparing the effects of the fluorescence microscopic examination among different redying reagents .Results The detection rate of Mycobacterium tuberculosis was 66 .67%(132/198) for the Ziehi‐Neelsen staining ,94 .9% (188/198) for the fluorescence stainings and 94 .95% (188/198) for the methyl‐ene blue staining ,in which the detection rate of methylene blue redyeing was 94 .95% ,which of hematoxylin redyeing was 94 .44%(187/198) and which of potassium permanganate redyeing was 94 .44 (187/198) ,the differences among them were statistically sig‐nificant(P< 0 .05) .Conclusion The fluorescent staining method has the higher positive detection rate of Mycobacterium tubercu‐losis than the Ziehl‐Neelsen staining method ,in which 0 .3% methylene blue solution is a good background quenching redyeing solu‐tion .

ObjectiveTo observe the effect of integrated traditional Chinese medicine therapy on pelvic inflammation. Methods40 patients of chronic pelvic inflammation were as the group of integrated traditional Chinese medicine therapy and received Chinese herbal medicine treatment (by taken orally, clysis and hot compress), acupuncture, mental state therapy etc. Other 36 cases were as the control group and only received Chinese herbal medicine treatment by taken orally. Then effects of two groups were compared. ResultsIt shows that the group of integrated traditional Chinese medicine therapy got obviously better results than the control group (P<0.01). ConclusionsThe integrated traditional Chinese medicine therapy is more effective on chronic pelvic inflammation.

Animal Diseases ; epidemiology ; prevention & control ; virology ; Animals ; China ; epidemiology ; Humans ; Infection Control ; methods ; Rabies ; epidemiology ; prevention & control ; veterinary ; virology ; Rabies Vaccines ; administration & dosage ; Rabies virus ; immunology

Microbubble contrast agents for ultrasound (US) have been developed and clinically applied in recent years. Contrast-enhanced ultrasound (CEUS) has been widely used in the imaging of liver and other organs such as kidney, pancreases, spleen, prostate, ovarian, uterus as well as abdominal trauma, showing promising values in the diagnosis and differential diagnosis of various diseases. This article reviews the recent development and future protential clinical applications of CEUS.
Contrast Media ; administration & dosage ; Diagnosis, Differential ; Drug Delivery Systems ; Humans ; Microbubbles ; Ultrasonography ; methods

Objective To observe the ultrasonographic features of local chest wall tumor recurrence after mastectomy for breast cancer and its clinical and histopathological characteristics. Methods The ultrasonographic features, clinical and histopathological characteristics of 27 patients with local chest wall tumor recurrence after mastectomy confirmed histopathologically were retrospectively reviewed. Results The disease-free intervals of twenty-seven patients ranged from 3 to 129 months [mean (31.9±31.4) months]. Most of the recurrence(18/27, 66.7%) occurred within 3 years after mastectomy. The clinical manifestations were:7 cases (7/27, 25.9%) with regional skin redness and swelling, red rash or ulceration on chest wall associated with or without palpable mass, 20 cases(20/27, 74.1%) with chest wall palpable masses without obvious skin change. On ultrasonography, 2 cases showed diffuse inifltrative type with ill-deifned inhomogeneous hypoechoic lesion and skin thickening. And twenty-ifve cases(43 lesions) showed mass type with a lesion size range of 5.4-114.7 mm [mean (24.4±21.6) mm]. Among them, 32 lesions were located near to the operation incision scar, 36 involved muscle layer, 38 were hypoechoic, 31 had irregular shape, 24 had indistinct margin, and 31 had blood lfow signal. In addition, calciifcation, halo, and taller-than-wide shape were absent in all 43 lesions. Conclusions The tumor recurrence often occurred within 3 years after mastectomy in high-risk patients. Ultrasonographic feature of chest wall recurrent lesion is of great value in the diagnosis.

Objective To assess the quality of iodized salt and investigate the urinary iodine level of pupils and women after implementation of universal salt iodization in Shijiazhuang city. Methods Between 2004 and 2008, nine salt samples were randomly collected from wholesale enterprises of 5 different directions of east,south, west, north and center in Shijiazhuang city every month. Meanwhile, eight salt samples, four urine samples of women, were randomly collected in each of the 8 households of 210 villages selected from 23 counties. Between 2004 and 2008, twenty urine samples of 8 - 10 year old pupils were collected in each of 160 schools from the 23 counties. Direct titration method was used for salt iodine determination, iodine concentration in urine was detected by the method of ammonium persulfate digestion-As3+-Ce4 + catalytic spectrophotometry. Results At wholesale level, the qualified rate of iodized salt was above 99%. At household level, the consuming rate of iodized salt was above 95%, and the rate of consuming non-iodized salt was less than 5%. The rate of qualified iodized salt was above 90% in every year except 2004(87.10%), and the rate of consuming qualified iodized salt was above 90% in those years except 2004(83.08%). Urinary median iodine level of both the pupils and the women was higher than 100 μg/L, and the rate of urinary iodine level that less than 50 μg/L was below 10%. Conclusions The quality assessment of iodized salt from wholesale companies and related indices of urinary iodine level of pupils, women and household iodized salt have already reached the national standard for eliminating iodine deficiency disorders.

ObjectiveTo study the effect of hyperbaric oxygen therapy on the cholinergic neurons of hippocampus and praxiology of vascular dementia rats.MethodsThe vascular dementia models were made and divided into control and therapy group. After 30 days of HBO therapy, abilities of learning and memory of rats were tested by using the Morris water maze. Immunochemistry staining was used to observe the number of cholinergic neurons of CA1 subfield of hippocampus. ResultsAbilities of learning and memory of rats and the number of the neurons positive to ChAT like immunoreaction in the CA1 subfield of the hippocampus significantly increased in therapy group.ConclusionHyperbaric oxygen therapy is effective on the vascular dementia model rats.

Adult ; Aged ; Female ; Follow-Up Studies ; Humans ; Male ; Middle Aged ; Recurrence ; Retinal Detachment ; etiology ; surgery ; Silicone Oils ; therapeutic use