Objective:To study the effects of LAMS on modulation of HL-60 cell strain.Methods:MTT was used to determine the inhibiting action in the different concentration of LAMS on HL-60 cell strain.The attitude of Sub-G1 peak in DNA histogram was examined by FCM.NF-?B expression affected by LAMS was determined by using immunohistochemical stainings.Results:With the increase of LAMS concentration,the inhibiting effects on multiplication of HL-60 cells were decreased.After treatment of HL-60 cells with 0.75?g/ml LAMS for 6h?12h?24h,the expression of P65 was the most significant at 12h,while the expression of IKb? was the most significant at 6h.The exptession of NF-?B reduced in LAMS group without time-dependent pattern.Conclusion:LAMS can induce HL-60 cells apoptosis.LAMS can modulate the expression of NF-?B.It also suggested that LAMS had an influence at molecular level.

Objective:To explore the histopathological change of the fragrant pig skin induced by focus ultrasound repetition irradiation to offer experiment basis for the safety and effectivessness of clinically treating postherpetic neuralgia(PHN) by repetition irradiation.Methods:The pig skin was irradiated once every other day,altogether three,with irradiation power as 2W,and frequency as 10.06MHz,The skin change was observed under light microscope and electron microscope.Results:Hyperemia and edema in irradiation area after irradiation were observed instantly to the eye;Under light microscope there were hyperemia and edema in epidermis,angiotelectasis and inflammatory infiltrating,and find coagulative necrosis in slice was not observed.Under electron microscope,instantly after ultrasound irradiation every other day,endothelial cell of blood vessel can be found mitochondria swelling and more micropinocytotic vesicles,mitochondria swelling and rough endoplasmic reticulum dilatation were noted for in fibroblasts,and mitochondfia swelling for nerve fibre.Conclusions:(1)There is reversibile lesion in epidermis under this dose of ultrasound.(2)Skins don’t accumulate lesion every other day after irradiation repeatly .which illustrates that it is effective and safe to treat postherpetic neuralgia by ultrasound irradiation repeatly.

In this paper, LM35 tempeture sensor is tested and shows good linear characteristics between 30 ℃ and 60 ℃, which provides a theoretical basis for making digital thermometer. A/D converting chip and 89c51 single chip micro -processor are employed in this digital body thermometer based on LM35. It possesses such advantages as low cost, high accuracy and excellent stability.

Scientific and technological cooperation plays a key role in improving innovative capabilities and speeding up achievement transformation. Promoting self-discipline in morality, strengthening restraint by rules and regulations and enhancing legal protection of intellectual property are some of the key links in perfecting the credit system and consummating large-scale scientific and technological cooperation. Adopting the "coordinate signature system" for significant achievements through large-scale scientific and technological cooperation is the driving force behind such cooperation. Establishing information and experimental technology platforms is the basis and supplementary, means of scientific and technological cooperation while setting up a talent flow and excitation system and perfecting the system of resources integration and sharing are beneficial to the creation of a sound environment for large-scale scientific and technological cooperation.

Objective: To employ the subtraction technique of 64-slice spiral CT angiography for intra- and extra-cranial arteries imaging, so as to study the managing method and assess the clinical outcome. Methods: The pre-contrast and post-contrast images of 100 patients were obtained by 64-slice spiral CT angiography. A special head holder was used during the scanning to guarantee the accurate overlapping of the pre-contrast and post-contrast images. A reconstructed three-dimensional model of the pre-contrast bone dataset was subtracted from the post-contrast dataset to produce the subtracted images. (1) An experienced operator performed the conventional and subtraction reconstructions; the periods for image generation of both methods were recorded and compared. (2) The quality of the images were evaluated and graded (grade 1-5) by 2 experienced radiologists in a double-blinded manner. Results: (1) The new subtraction technique using 64-slice spiral CT angiography needed significantly less time than the conventional method did (11.01±1.89 min vs 14.30±2.25 min, P<0.01). (2)The correlation coefficient of the results of 2 radiologists was 0.87. The score of image quality by subtraction 64-slice spiral CT angiography was significantly higher than that by the conventional method (4.22±050 vs 2.93±0.36, P<0.05). Conclusion: Subtraction 64-slice spiral CT angiography is easy to use and time saving for intra- and extra-cranial artery imaging; it does not has the difficulty of separating artery from bone and is worth popularizing in clinic.

Objective To evaluate the cranioplasty with Titanium mesh.Methods Clinical studies of 110 cases who accepted cranioplasty with Titanium mesh (2000-2001) were reviewed retrospectively.Results A satisfactory moulding with no complication occurred in 91%(100/110). The most common recent complication of cranioplasty with Titanium was subcutaneous hematoma(19/110); the next was epidural hematoma(3/110), intracerebral hematoma(3/110) and brain contusion(1/110). 69 cases more than 1 year after cranioplasty were followed, no one appeared long term complications such as infection, exposure or mobilization of the mending material.Conclusion The results of cranioplasty with Titanium mesh are satisfactory, the recent complications of the operation are mainly related to incompletely hemostasis, the long term complication occurred rarely.

OBJECTIVE: To establish an LC-MS method for simultaneous determination of six compounds (uridine, guanosine, ferulic acid, ligustilide, senkyunolide I and II in Shunaoxin dropping pills. METHODS: The analysis was performed on a Waters Acquity BEH C18 column (2.1 mm × 100 mm, 1.7 μm) with the mixture of acetonitrile-water-formic acid as the mobile phase, and the flow rate was 0.4 mL · min-1; the PDA detection wavelength was 190-400 nm; the column temperature was room temperature (30°C). RESULTS: The linear ranges for uridine, guanosine, ferulic acid, ligustilide, senkyunolide I and H were 10.31-206.24, 3.28-65.52, 6.36-127.24, 8.24-164.88, 10.18-200.52, and 3.14-62.72 μg · mL-1, respectively. The average recoveries were between 99%-101% (n=6). CONCLUSION: This method is simple, accurate and reliable to determine the contents of the six compounds in Shunaoxin dropping pills for the quality control.

OBJECTIVE: To develop an HPLC method for simultaneously determining the contents of naringin and neohespiridin in rat plasma, and to investigate the pharmacokinetics and relative bioavailability of self-microemulsifying pellets of daidai flavones in rats. METHODS: The rats were divided into two groups randomly. They were administered orally suspension of daidai flavones effective parts and its self-microemulsifying pellets, which both contained 120 mg · kg-1 of flavonoids. Blood samples were collected at 0.083, 0.167, 0.333, 0.5, 0.667, 1, 2, 4, 6, 8, 10, 12 and 24 h and the concentrations of naringin and neohesperidin in rat plasma were simultaneously determined by HPLC. The pharmacokinetic parameters were calculated by DAS 3.0 pharmacokinetic program. RESULTS: The pharmacokintic parameters of naringin in the self-microemulsifying pellets of daidai flavones and the suspension were as follows: AUC0-24 (17.710 ± 6.588) and( 9.139 ± 1.982) mg · h · L-1, ρmax (4.816 ± 1.329) and (1.572 ± 0.324) mg · L-1, tmax (0.611 ± 0.086) and (1.575 ± 0.324)h, and t1/2 (13.078 ± 6.382) and (11.678 ± 6.919)h, respectively. And the pharmacokintic parameters of neohesperidin were as follow: AUC0-24 (18.094 ± 4.892) and (10.961 ± 2.276)mg · h · L-1, ρmax (5.657 ± 1.391) and (2.096 ± 0.512) mg · L-1, tmax(0.583 ± 0.091) and (0.806 ± 0.222) h, and t1/2 (14.606 ± 7.562) and (10.985 ± 6.963)h, respectively. The relative bioavailabilities of naringin and neohesperidin in the self-microemulsifying pellets of daidai flavones were 193.78% and 165.08%, respectively. CONCLUSION: The bioavailability of daidai flavones is increased by making them into self-microemulsifying pellets, and the biopharmaceutical property of the flavones is improved.

Objective: To explore the efficacy of mild moxibustion plus loratadine tablets for children with allergic rhinitis (AR).Methods: A total of 80 children were randomized into a control group and an observation group, with 40 cases in each group. The control group was treated with loratadine tablets, and the observation group was treated with mild moxibustion plus loratadine tablets. Before and after treatment, the total nasal symptom score (TNSS) was evaluated, and the serum eosinophils (EOS) count, and the interleukin (IL)-27 and macrophage migration inhibitory factor (MIF) levels were measured. Clinical efficacy was evaluated after treatment. Results: The total effective rate of the observation group was higher than that of the control group (P<0.05). After treatment, the TNSS in both groups decreased (P<0.05), and the TNSS in the observation group was lower than that in the control group (P<0.05); the serum EOS count in both groups decreased (P<0.05), and the serum EOS count in the observation group was lower than that in the control group (P<0.05). The serum IL-27 level in the control group had no statistical difference compared with the same group before treatment (P>0.05), and the serum MIF level decreased after treatment (P<0.05). The serum IL-27 level in the observation group increased after treatment (P<0.05), and the serum MIF level decreased after treatment (P<0.05), and were both statistically different from those in the control group (P<0.05). Conclusion: Mild moxibustion plus loratadine tablets is effective in treating children with AR. It can significantly improve the nasal symptoms and reduce the serum EOS count, which may be related to the regulation of the serum IL-27 and MIF levels.

OBJECTIVETo measure the changes of plantar pressure of the first tarsometatarsal joint fracture and dislocation by three different implants to provide experimental reference in selecting implants.

METHODSEight fresh foot specimens were made into the models of the first tarsometatarsal joint fracture and dislocation, which were fixed with 3.5 mm cortical screw, 1/4 tubular plate and compressive staple in turn. After the loading of 600 N, the changes of the plantar pressure in forefoot were measured by the method of the F-scan plantar pressure system.

RESULTSAfter first tarsometatarsal joint fracture and dislocation, the peak pressure under the first metatarsal head would decrease, while the pressure under the second metatarsal head would increased,whose differences were statistically significant (P<0.05). When the first tarsometatarsal joint was fixed with screw or plate respectively; the peak pressure under the two metatarsal heads would tend to be normal. However,the staple fixation showed the statistical significant difference compared with normal state, although the peak pressure under the first and second metatarsal heads were recovered in some extent(P<0.05).

CONCLUSIONAfter the first tarsometatarsal joint fracture-dislocation, the plantar pressure might be compensated partly by the adjacent metatarsal heads according to the regulation of the load transfer mechanism. While the first tarsometatarsal joint fracture-dislocation was fixated by screw or plate, the plantar pressure of the forefoot would return to the normal state. However,if the joint was fixated by the staple, it would still be difficult to return the plantar pressure to be normal.

Foot Joints ; injuries ; surgery ; Forefoot, Human ; physiology ; Fracture Fixation, Internal ; methods ; Fractures, Bone ; surgery ; Humans ; Joint Dislocations ; surgery ; Pressure