ObjectiveTo explore the clinical features of senile coronary heart disease.MethodsClinical data of 367 cases with senile coronary heart disease admitted from January 2001 to December 2004 were reviewed.ResultsOf the 367 cases with senile coronary heart disease, 42 were asymptomatic, 92 were angina pectoris, 56 were myocardial infarction, 66 were arrhythmia, 74 were angina pectoris or myocardial infarction with arrhythmia, 37 were angina pectoris or myocardial infarction with cardiac insufficiency. Of the 79 cases with myocardial infarction, 47 were myocardial infarction with Q wave, 32 were myocardial infarction without Q wave. Of the 140 cases with arrhythmia, many cases were varied arrhythmias, 51 were atrial flutter or atrial fibrillation, 37 were ventricular premature beat, 34 were atrial premature beat, 27 were intraventricular block, 12 were atrioventricular block, 8 were sick sinus syndrome.ConclusionThe clinical features of senile coronary heart disease manifested asymptomatic, atypical angina pectoris, arrhythmias, cardiac insufficiency, varied coronary heart disease, myocardial infarction are myocardial infarction without Q wave, etc.

Multiple organ dysfunction syndrome(MODS) is an important area of critical medicine study.Establishing a suitable animal model is one of the important manner in the study.This article has reviewed the choice of animal,assessment of model and the method of making model.

Objective :To study the effect of MMP-1,TIMP-1,VEGF and t-PA,PAI-1 in the inflammatory process of endometrium cell and the active components of SQ.Methods: ICC,ISH and WB was used to test the level of protein of MMP-1,TIMP-1,VEGF and t-PA,PAI-1 and mRNA of MMP-1,TIMP-1 and VEGF of endometrium cell in regular group,pattern group,GongXueNing group and SQ group.Result: In contrast with model group,the level of MMP-1protein and mRNA expression obviously decreased,but the level of TIMP-1 and VEGF increased in SQ group,there were signifi cant differences(P

Objective To study the DNA damage effect of the organic extracts from Chlorinated domestic sewage coming from three different places in vivo and in vitro. Methods The domestic sewage samples were collected from different places:university A, university B and a hotel. The DNA damage effect was tested in the bacteria, cells and BALB/C mouse by single cell gel electrophoresis (comet assay), the micronucleus test, Ames test. Results Comet assay indicators:all three samples, before chlorinated, at the dosages of 1.0, 5.0 mg/ml, after chlorinated, at the dosages of 0.04, 0.2, 1.0, 5.0 mg/ml for university A, 0.2, 1.0, 5.0 mg/ml for a hotel, 1.0, 5.0 mg/ml for university B, were significantly higher than those in the negative group(P

The macroscopy and ultrasondgraphy analysis for thirty reseeted specimens of esophageal carcinoma proved by pathology was reported in this paper.The ultrasonic features of esophageal carcinoma as follows:the local wails became thicker,their layers were unclear or disap-peared,the normal walls were insteaded of irregular masses with hypoechoic patterns, the borders of the masses were definite or indefinite,and the posterior echoes of the masses had little change.In addi-tion,the depth of tumor invasion Was compared by the ultrasonography and pathology.The ultrasonic features of the esophageal walls without tumor were also described.

Objective To explore the role of mouse dermis-derived mesenchymal stem cells (mdMSC) on skin repair. Methods mdMSC and human dermal fibroblasts were isolated and identified. Human dermal fibroblasts were cultured alone or eoeultured with mdMSC in Transwell chambers with the density ratio of human dermal fibroblasts to mdMSC being 2/5, 1/1, and 2/1. On day 4 and 8 of culture, the expression levels of hydroxyproline and transforming growth factor-beta (TGF-beta) 1 were measured in the supematant of monoculture and coculture by alkaline hydrolysis and ELISA respectively. Results The level of hydro-xyproline was significantly higher in the supematants of coculture system with a density ratio of 2/5 and 1/1 than that in monoculture supematants of human dermal fibroblasts on day 8 (both P < 0.05). Elevated level of TGF-betal was observed in all coculture supematants on day 8 (all P < 0.01) and in the supernatants of coculture system with a density ratio of 1/1 on day 4 (P < 0.05). There was no significant correlation between the expression level of TGF-betal and hydroxyproline in the coculture supernatants (r = 0.108, P ＞ 0.05). Conclusion In vitro coculture with mdMSC can increase the production of hydroxyproline and TGF-betal by fibroblasts, which may be a mechanism underlying the facilitation of skin repair by mdMSC.

Objective To describe the sonographic features of abdominal wall endometriosis(AWE).Methods 105 consecutive women with proven pathological endometriosis of the abdominal wall were retrospectively recruited.The clinical data and the result of the sonographic examinations were reviewed and described.The AWE lesions were divided into ＜3 cm and ≥3 cm groups according to their maximum diameter.Results The sonographic feature of AWE includes irregular hypoechoic nodules with ill-defined margins,anechoic and hyperechoic ring maybe exist.In color Doppler,most of them showed few internal vascularity.Compared with≥3 cm group,＜3 cm group nodules manifested as shorter latent period,with more superficial location,round or oval shape,peripheral hyperechoic ring and none or few vascularity were more common.As for ≥3 cm group nodules manifested as longer latent period,with deeper location,irregular shape,abundant of vascularity were more common.Conclusions Specific diagnosis of AWE can be made by using high frequency sonography combined with clinical features.More accurate information can be provided by judging the size,range and infiltrative depth.

Objective To establish the quality standard for Tongluo Tangtai Granules. Methods TLC was applied to the qualitative identification of Rhizoma Coptidis, Semen Sinapis Albae and Radix Scrophulariae, and HPLC was applied to the quantitative determination of berberine in the prescription. Results Rhizoma Coptidis, Semen Sinapis Albae and Radix Scrophulariae could be detected by TLC, the spots were clear and there was no interference for the negative sample. A good linearity of berberine was showed in the range of 0.037 29-2.610 3 μg (r=1.000 0). The average recovery of berberine was 98.85%, and RSD was 0.70% (n=6). Conclusion The methods are simple, sensitive, accurate with good reproducibility. It can be used for the quality control of Tongluo Tangtai Granules.

Objective To screen for differentially expressed proteins in sera from patients with common types of psoriasis,and to identify plasma protein markers for psoriasis.Methods Serum samples were collected from 6 patients with progressive psoriasis vulgaris,5 patients with erythroderma psoriaticum,and 6 healthy human controls,and then pooled into 3 pools:psoriasis vulgaris pool,erythroderma psoriaticum pool and control pool.After removal of high-abundance albumin and IgG,the pooled samples were analyzed by two-dimensional electrophoresis (2-DE).An electrophoretic gel image analysis software was used to locate differentially expressed protein spots followed by peptide mass fingerprinting with matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS).The National Center for Biotechnology Information (NCBI) protein databases were then searched for the identification of differentially expressed proteins.Results All the three pooled serum samples were well seperated by 2-DE.As the gel image analysis software showed,there were 33 protein spots differentially expressed between the patients with psoriasis vulgaris and the healthy controls,17 between the patients with erythroderma psoriaticum and the healthy controls,and 26 between the patients with psoriasis vulgaris and those with erythroderma psoriaticum.Finally,14 proteins were identified as differentially expressed proteins.The patients with psoriasis vulgaris showed higher expression of complement component 3,interleukin-16,vitamin D-binding protein and α1-antitrypsin compared with the healthy controls; the patients with erythroderma psoriaticum showed increased expression of complement component 3,complement component H,α1-antitrypsin,hemopexin and haptoglobin,but decreased expression of serum amyloid protein compared with the healthy controls,as well as enhanced expression of α1-antitrypsin,complement component H,complement component 4 and haptoglobin compared with those with psoriasis vulgaris.Conclusion Differences exist in serum protein profiles between patients with psoriasis vulgaris and erythroderma psoriaticum,and healthy human controls.

ObjectiveOne hunred and twenty-four To probe into application value of ultrasound guided Catheterization in internal jugular vein. MethodsOne hundrel and twenty-four guided by ultrasound puncture holder, single-channel or three-channel catheter was embedded into jugular vein. ResultsThe catheter was inserted successfully by only one procedure in all the 124 patients. There was no complication. ConclusionsThe catheterization becomes safer, convenient and quickly with ultrasound puncture holder guidance, decreasing failure rate and complication of the procedure.