1.Effect of Lappaconitine on Postoperative Pain and Serum Complement 3 and 4 Levels of Cancer Patients Undergoing Rectum Surgery.
Chinese Journal of Integrated Traditional and Western Medicine 2015;35(6):668-672
OBJECTIVETo explore the effect of lappaconitine on patient-controlled intravenous analgesia (PCIA) and serum complement 3 and 4 (C3 and C4) levels of cancer patients undergoing rectum surgery.
METHODSTotally 60 patients, who were scheduled for rectum carcinoma surgery, were recruited to the study and assigned in 3 groups, the blank control group, the tramadol group, and the lappaconitine group, 20 in each group. Lappaconitine (8 mg) was intravenously dripped to patients in the lappaconitine group 30 min before ending the operation. PCIA started as soon as the end of the surgery and the total dose of lappaconitine was 36 mg. Patients of the tramadol group were treated with tramadol (100 mg) intravenously within 30 min before ending the operation. The dripping was completed within 30 min. PCIA was started as soon as the end of the surgery and the total dose of lappaconitine was 36 mg. Tramadol (100 mg) was intravenously dripped to patients in the tramadol group 30 min before ending the operation. PICA was started as soon as the end of the surgery and the total dose of tramadol was 900 mg. Pethidine (50 mg) and droperidol (2. 5 mg) was intramuscularly injected to patients in the blank control group for pain relief according to their complaints. Pain degrees were assessed by visual analog scale (VAS) 12 h before surgery, 12, 24, 48, and 72 h after surgery. Blood samples were withdrawn at the same time point. Contents of serum C3 and C4 were determined by immunoturbidimetry.
RESULTSVAS scores of the blank control group were significantly higher after surgery than before surgery (P <0. 01). There was no statistical difference in VAS scores between before surgery and after surgery in the tramadol group and the lappaconitine group (P >0. 05). VAS scores were significantly lower at each post-surgery time point in the tramadol group and the lappaconitine group than in the blank control group with statistical difference (P < 0.01). There was no statistical difference in VAS scores at each post-surgery time point between the tramadol group and the lappaconitine group (P >0. 05). Compared with before surgery, contents of serum C3 and C4 significantly decreased in all of the three groups at 12, 24, and 48 h after surgery (P < 0.05, P < 0.01). They recovered to the pre-surgery level till 72 h after surgery (P > 0.05). Serum C3 and C4 contents at 48 h after surgery were higher in the tramadol group than in the blank control group (P < 0.05). Serum C3 and C4 contents at 24 and 48 h after surgery were higher in the lappaconitine group than in the blank control group (P < 0.05). There was no statistical difference in serum C3 and C4 contents at each time point between the tramadol group and the lappaconitine group (P > 0.05). VAS scores were obviously negatively correlated with serum contents of C3 and C4 (r = -0.622, r = -0.649, P < 0.01).
CONCLUSIONSLappaconitine (used at the dose in this study) showed better pain relief effect after surgery. Besides, it could inhibit the surgic wound and pain, and elevate serum contents of C3 and C4.
Aconitine ; analogs & derivatives ; therapeutic use ; Analgesia, Patient-Controlled ; methods ; Analgesics, Non-Narcotic ; therapeutic use ; Complement C3 ; metabolism ; Digestive System Surgical Procedures ; Humans ; Neoplasms ; Orthopedic Procedures ; Pain Measurement ; Pain, Postoperative ; Postoperative Period ; Rectum ; surgery ; Tramadol
2.Quality Control of Plasma Lipids Measurement in CNHS 2002
Jian ZHANG ; Hong LI ; Qing-Qing MAN ;
Chinese Journal of Prevention and Control of Chronic Diseases 2006;0(05):-
Objective To describe the work of quality control for lipids measurement in CNHS 2002.Methods The quality control was applied over the whole project including staff training,making standard operating procedure(blood collection,separation,temporary storage,transportation,final storage),lab materials assignment,internal quality control in central lab and participating US Disease Control Center's Lipids Standard Program(LSP)as external quality control.Results Seven times of national training programs were held and 326 technicians from CDCs in 31 provinces,autonomous regions, municiplalities and 132 surveyed counties received related trainings.During the program,321 persons passed examination (98.5%)and among them 205 got the A score(62.9%);The field work was implemented strictly according to the procedure. Three automatic biochemical instruments were used in the measurement and there is no significant difference between means of serum TC,TG and HDL-C from several batches of quality control serum.Parallel measurements were conducted in five percent randomly selected samples,the ratio of bias less than 3% was 99.05%,96.40% and 98.30% for TC,TG and HLD-C, respectively.Compared with means of LSP control sera,the results showed that all bias was less than 5% except one bias of TG result(7.02%)in one batch of LSP controls.Conclusion The work of quality control in this survey guaranteed the accuracy of plasma lipids measurement,and provided the basic data for the epidemiological description of dyslipidemia status among Chinese and the further analysis.
4.Effect of myofibroblast accumulation on the formation and development of nasal polyps.
Qiu-ping WANG ; Man-jie JIANG ; Ze-qing LI
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2006;41(5):381-383
Adult
;
Connective Tissue Cells
;
Female
;
Humans
;
Male
;
Middle Aged
;
Myofibroblasts
;
cytology
;
Nasal Polyps
;
pathology
;
Young Adult
5.Comparison of MRI and arthroscopy on defect area measurement of knee joint cartilage
Yulin MAN ; Youmin GUO ; Xiaobin LIU ; Qing SUN ; Ying WANG
Journal of Practical Radiology 2015;(1):113-115,120
Objective To explore the consistency of knee articular cartilage defect area by magnetic resonance imaging (MRI)and arthroscopy.Methods Thirty-six patients (36 knees)with knee cartilage lesions were collected in our study.The defect area meas-urement of knee articular cartilage by MRI and arthroscopy was compared.Results A total of 92 lesions were detected by both MRI and arthroscopy with an average of 2.6 defects per knee.Preoperative MRI resultes demonstrated the mean defect area per knee was (1.9±1.5)cm2 ,while arthroscopic measurement after debridement indicated the mean defect area per knee was (2.9±2.6)cm2 , which had significant difference(P <0.001).The area measured by MRI was less than that by arthroscopy,and the mean difference was (1.7 ± 1.1)cm2 .The mean area measured by MRI was 70.1% of that by arthroscopy.Conclusion Arthroscopy and MR have good consistency in the number of articular cartilage defect lesions .MRI can reflect the knee articular cartilage defect area directly or indirectly,and be capable of accurate positioning and qualitative diagnosis.
7.A New Rapid Method for Preparing Synaptosomes of Mouse
Zhongxin XIAO ; Shougang WEI ; Shaojun YUN ; Xiaojuan HE ; Shuhua ZHANG ; Qing CAI ; Qiang LU ; Man JI
Chinese Journal of Rehabilitation Theory and Practice 2010;16(10):937-938
ObjectiveTo establish a new method for preparing synaptosomes.MethodsDensity gradient centrifugation method was used to isolate synaptosomes of mouse, checking by transmission electron microscopy.ResultsSynaptosomes prepared by this method had intact morphological characteristics, surrounding with a continuous oval-shaped membrane structure, moreover, mitochondrion and lots of synaptic vesicle in them.ConclusionThis method is applicable to establish a rapid, convenient and useful method for preparing synaptosomes.
8.Research progress on targets for analgesics
Chinese Pharmacological Bulletin 2018;34(2):161-165
Pain as a clinical common symptom is one of the most serious problems to threaten human health,therefore,pain management is one of the main aspects of clinical medication.This review briefly described the existing and the novel analgesic targets,in order to study and develop new kinds of analgesic drugs with high efficacy,less side effects and no resistance and addiction,which aims to provide a reference for the clinical application.
9.Expression of StAR mRNA in the Early Piglet Testes
Xiao-Qing ZHANG ; Xian-Zhong WANG ; Yan SUN ; Man-Yi WANG ; Jia-Hua ZHANG ;
China Biotechnology 2006;0(03):-
The expressions of StAR (steroidogenic acute regulatory protein) mRNA in testes from 7,14,23 and 37 day-old piglets were studied by tissue in situ hybridization. The results indicated that in the testes of piglets, StARmRNA was expressed in Leydig cells of pig testes. The expression level of StARmRNA was lower in 7 days piglets but higher in 14,23,37 days. The results indicated that StAR gene played an important role in steroid biosynthesis.
10.Assessment and direction of Objective Structured Clinical Examination on clinical practice in Department of Obstetrics and Gynecology
yue-zhen, XUE ; zhi-feng, SHI ; ping, LI ; li, MA ; man, LUO ; yang, ZOU ; qing, MIAO
Journal of Shanghai Jiaotong University(Medical Science) 2008;0(S1):-
Objective To analyze the results of the Objective Structured Clinical Examination(OSCE) of medical interns in Department of Gynecology and Obstetrics,and to assess the role of OSCE in clinical teaching and practice. MethodsAtolal of 104 medical interns of five-year education program in 2002 and seven-year education program in 2003 in School of Medicine had taken part in OSCE of gynecology and obstetrics.The OSCE consisted of six stations: gynecologic examination,obstetric examination,oral test,interrogation of standardized-patients(SP),drawing partogram,and non-stress test(NST) explanation.After gathering the data of each station,the software of SPSS 11.0 was used to make statistical analysis. Results The median scores were as follows: 92.0 for gynecologic examination,91.0 for obstetric examination,83.5 for oral test,80.0 for interrogation of SP,80.0 for drawing partogram,and 70.0 for NST explanation.There was no significant impact of familiarity to OSCE on the results of the test.The results showed that medical interns did good job in basic performance,but lack of clinical practice skills in some complicated practice of gynecology and obstetrics.Conclusion The grading system and stations setup of OSCE in Department of Gynecology and Obstetrics in Shanghai Sixth People's Hospital show objectivity and equity.The OSCE can be widely applied in the after-department examination and even graduation examination.