Objective To study the mechanism of the extract of three kinds of Chinese herbs in treating psoriasis.Methods Three kinds of Chinese herbs(coptis root,patience dock root and atratylodes rhizome) were extracted.With the culture of human transformed keratinocyte cell line colo-16 cells as a model,the single and compound effects on the growth of KC were studied before and after stimulated by TNF-?.Results The single and compound extract of three Chinese herbs could restrain the proliferation of KC before and after stimulated by TNF-?.The effect was more remarkable than the compared methotrexate and cyclosporin A(P

Background:The efficacy of traditional medicine on ulcerative colitis (UC) is often unsatisfactory, hence development of drug based on the pathogenic mechanism of UC becomes a hot topic in the research of UC.It has been revealed in recent studies that activation of nuclear factor-κB (NF-κB) is implicated as a key regulator in the immune and inflammatory responses in UC.Aims:To explore whether bortezomib, a potent proteasome inhibitor that inhibits NF-κB activation can be used for treatment of UC.Methods:Thirty-two BALB/c mice were used to induce acute experimental colitis by drinking 3%dextran sulfate sodium (DSS) freely for 7 days, and then randomly allocated into four groups injected intraperitoneally with 0.2 (low-dose group), 0.6 (medium-dose group), 1.0 mg/kg (high-dose group) bortezomib and normal saline (model control group), respectively.On the 7th day after treatment, the disease activity index (DAI) and histopathological change of colonic tissue were observed;the colitis-related parameters including peripheral blood hemoglobin (Hb), C-reactive protein (CRP) and colonic myeloperoxidase (MPO) activity were measured, and the nuclear translocation of NF-κB was assessed by electrophoretic mobility shift assay.Results:Compared with the model control group, the DAI, CRP, MPO activity, and injury score of colonic tissue were decreased gradually, and the Hb was increased gradually in mice treated with low-, medium-and high-dose bortezomib (P all <0.05).The efficacy of medium-and high-dose bortezomib was notable.In mice treated with medium-and high-dose bortezomib, nuclear translocation of NF-κB was inhibited obviously.Conclusions:Bortezomib can modulate the colonic inflammation in mice with experimental colitis by inhibiting NF-κB activation and subsequently improving the clinical manifestations, colitis-related parameters and tissue damage.Increasing the dosage of bortezomib in a safety range may enhance the treatment response.

Objective To investigate the effect of tramadol on perioperative insulin resistance in patients undergoing surgical operations with different trauma.Methods One hundred and twenty patients who had general and gynecology belly all numb surgical operation were divided into tramadol group and control group,each with 60 cases.Five minutes before skin incision,the tramadol group was given tramadol 1.5 mg/kg intravenously.followed immediately by a continuous infusion of 0.5 mg/kg per hour until the end of surgery,and the control group was given normal saline instead of tramadol.Blood samples were taken from vein for determination of plasma blood sugar,insulin concentrations before induction of anesthesia(T0),at the end of the operation(T1)and seven o'clock on the next morning(T2).The value of ISI Was calculated.Grading upon the trauma degree of surgical operation was divided into three groups(Ⅰ,Ⅱ,Ⅲsubgroup).Results The concentrations of blood sugar and insulin at Tohad no significant difference in Ⅰ,Ⅱ,Ⅲsubgroups in the tramadol group(P＞0.05).The concentrations of blood sugar and insulin at T1,T2 were increased significantly compared to T0 in each subgroup[To:(4.74 ±0.43)mmol/L,(15.25±1.22)U/L;T1:(5.35±0.47)mmol/L,(17.74±1.45)U/L;T2:(6.55±0.53)mmol/L,(18.59±1.56)U/L](P＜0.05),ISI was decreased(P＜0.05).The concentrations of blood sugar and insulin at T1,T2 were significantly lower in the tramadol group than those in the control group.The value of ISI was significantly higher in the tramadol group than that in the control group(P＜0.05).Conclusions The insulin resistance presents relation with the surgical operation degree.The severe the wound is,the bigger insulin resistance is.Tramadol can alleviate perioperative insulin resistance in patients undergoing surgical operations.

Adult ; Female ; Granuloma ; etiology ; surgery ; Humans ; Male ; Middle Aged ; Postoperative Complications ; surgery ; Tracheal Diseases ; etiology ; surgery ; Tracheal Stenosis ; etiology ; surgery ; Tracheostomy ; adverse effects

Objective To assess the therapeutic efficiency of short anterior urethral stricture treated with endourethral surgery combined with iridium 192 radiation therapy in order to decrease the recurrence rate after endourethral surgery. Methods A total of 286 patients with anterior urethral stricture (stricture length ranging from 0.5 to 2.5cm, averaging at 1.6cm) were divided randomly into two groups after internal urethrotomy and cicatrix electrotomy. The radiation group of 173 patients was treated as soon as possible with iridium 192 radiation therapy on the stricture sections while the control group of 113 patients was not. The recurrence rate was compared between the two groups during one year's follow-up. Results Among all the 286 successful operations, stricture recurred in 19 patients of the radiation group (recurrence rate of 10.98%) and in 62 ones of the control group (recurrence rate of 54.86%), with a significant difference (P=0.003). Conclusion It is an effective way to prevent recurrence by treating patients as early as possible with iridium 192 radiation on the stricture sections after endourethral operation on anterior urethral stricture.

BACKGROUND:Methods after mechanical stimulation can be combined with various kinds of cells to produce many conversion types so as to start the various physiological functions of the body. OBJECTIVE:To analysis the reason why rol ing massage can improve the intracellular concentration of Ca2+METHODS:A biodynamic experimental device was set up, composed of pressure generating device, pressure forming device, pressure display device and related data recording device. We detected the effects of rol ing massage on Ca . 2+concentration in normal skeletal muscle cells and damaged cells through the different functions of the experimental device and system software. These functions consisted of pressure display, data transmission and recording, signal acquisition and processing, pressure signal acquisition, control and processing of signal acquisition, wireless signal transmission and receiving system, display system, storage system, system implementation function. RESULTS AND CONCLUSION:The experiment results showed that rol ing massage could reduce the Ca 2+concentration in the normal skeletal muscle cells and damaged cells. These findings suggest that rol ing massage can improve the overload of intracellular Ca2+in damaged cells, and it is superior to the sinusoidal mechanical stimulus.

The efficacy of growth hormone (GH) treatment in short children with small for gestational age (SGA) was evaluated and the association of serum insulin-like growth factor-1 (IGF) and IGF-binding protein-3 (IGFBP-3) levels with height increments was investigated.Fifty-five short children with SGA in our hospital from Sep 2009 to Sep 2013 were collected.Before GH treatment, insulin and arginine stimulation test were performed.The children were treated with recombinant GH with 0.15 U · kg-1 · d-1 for at least 12 months.After GH treatment, the height was (120.5 ± 17.63) cm, height SDS was (-1.53 ± 1.04) SDS, HtSDS was-2.04 ± 0.47, bone age was (6.19 ± 2.34) year, growth velocity was (7.24 ± 1.19) cm/year, all these parameters were significant higher than those before GH treatment.Pre-treatment serum levels of IGF-Ⅰ and IGFBP-3 were negatively correlated with post treatment growth velocity(r =-0.37 and r =-0.31;P =0.004 and P =0.021).GH treatment could effectively improve the height of short children with SGA.Children with lower pre-treatment serum levels of IGF-Ⅰ and IGFBP-3 may reach the higher growth velocity.

Objective To analyze the distribution and evolution of the risk factors for coronary heart disease (CHD) in middle-aging males, and provide the evidence for the prevention and treatment of male patients with CHD. Methods 1639 male people over 45 years old were enrolled in the study. They were divided into non- senile group (45-59 years old) and senile group (60-93 years old).The subjects over 60 years old were further divided into three groups:60-69 years old group, 70-79 years old group, 80-93 years old group. The interrelation between the risk factors of CHD and aging was analyzed. Results The prevalence rates of drinking, smoking, diastolic hypertension and low HDL-C in non-senile group were much higher than those in senile group (x2=28.80,18.35,15.72,17.84,P<0.01). In contrast, the prevalence rates of overweight, systolic hypertension, fasting hyperglycemia,postprandial hyperglycemia in senile group were significantly higher than those in non senile group. The prevalence rates of high triglyceride in non- senile group and senile group were 18.0% and 16.2% respectively(P>0.05). The prevalence rates of high cholesterol in non- senile group and senile group were 36.9% and 31.5% respectively(P>0.05). There was a significant decrease in the prevalence rates of overweight, postprandial hyperglycemia (P<0.05) and drinking,smoking, diastolic hypertension, high triglyeeride (P<0.01) in over 70-year+old males. And the prevalence rates of overweight, postprandial hyperglycemia, high triglyceride of over 80-year-old males were higher than those of over 70-year-old males (x2=10.05,4.16,5.97,P＜0.01). However,the prevalence rates of systolic hypertension of over 80-year-old males were lower than those of over 70-year-old males (X2=21.25,P＜0.01). There was no significant change in other risk factors of CHD at different ages of senile group. Conclusions For non-senile males, the important measures to treat the risk factors of CHD should include stopping smoking, restricting alcohol, eating low fat diet, changing bad life habits, controlling diastolic hypertension and modifying lipid. In contrast, for senile males, more attention should he paid to limiting total energy intake, increasing sports, losing weight, controlling systolic hypertension, hyperglycemia and modifying lipid.

Objective To investigate the application value of ganglioside treated for neonatal hypoxic-ischemic encephalopathy (HIE).Methods The 88 neonatal HIE children were divided into observation group and control group according to random digits table method with 44 cases each.The children in the two groups were given general comprehensive treated measures.The children in observation group were treated with ganglioside.The children in control group were treated with citicoline.The clinical treatment situation,clinical symptoms situation,neonatal behavioral neurological assessment (NBNA) score situation,the changes of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) levels in the two groups were compared and analyzed.Results The total efficiency in observation group was 90.91％(40/44),in control group was 68.18％ (30/44),there was statistically significant (P ＜ 0.01).The times of reflex recovery,muscle tension recovery and consciousness recovery in observation group were (7.04 ± 1.28),(8.13 ± 1.42) and (5.08 ± 0.90) d,in control group were (10.16 ± 1.57),(10.98 ± 1.33) and (9.76 ± 1.18) d,the targets in observation group were significantly shorter than those in control group (P ＜ 0.05).The change of NBNA score before and after treatment was (10.91 ± 1.83) scores in observation group,which was higher than that in control group [(9.07 ± 1.69) scores],and there was significant difference between the two groups (P＜ 0.05).The change ofTNF-α and IL-6 levels before and after treatment in observation group were (65.78 ± 12.25)and (88.58 ± 18.01) ng/L,which were lower than those in control group [(30.10 ± 11.93) and (32.69 ±17.94) ng/L],and there were significant differences between the two groups (P ＜0.05).Conclusions Ganglioside can be used as an ideal therapeutic drug for neonatal HIE to clinical application.Its mechanism may be related to the reduction of TNF-α and IL-6 levels.

Objective To construct mycobacterial membrane-anchored expression vector and to analyze expression level and sub-cellualr localization of exogenous target protein. Methods Based on the mycobacterial intracellular expression vector pMFA42 which contained a strong promoter of pfurAma mutant, the signal sequence of Mycobacterium tuberculosis(Mtb) 19×103 lipoprotein (19SS) was synthesized and was then cloned into the downstream of pfurAma mutant to generate the mycobacterial membrane-anchored expression vector pMFA42M. The coding gene of enhanced green fluorescent protein(EGFP) was amplified by PCR, and then sub-cloned into these two vectors described above to construct recombinant EGFP fused and membrane-anchored strains, respectively. The coding genes of Mtb immuno-dominant antigens Ag85A and its chimera Ag856A2 were then sub-cloned intothe membrane-anchored construct pMFA42MG to produce recombinant Mtb antigen EGFP fused-expression strains. After that, expression levels and sub-cellualr localization of exogenous target protein were further analyzed by Western blot and flow cytometry sorting(FCS), and the fluorescence intensities of recombinant EGFP- expressed strains were observed in vitro directly and after transfection of murine macrophage cell line RAW264.7. Results The novel mycobacterial membrane-anchored expression vector was constructed successfully by introduction of signal sequence of Mtb 19×103 lipoprotein. Using of EGFP as model antigen, exogenous target protein was demonstrated to be expressed with high level and could be anchored into cell membrane of recombinant mycobaterial strains. Conclusion A novel mycobacterial membrane-anchored expression vector was constructed successfully to research recombinant BCG and functions of mycobacterial membrane proteins, and the constructed EGFP-expressed recombinant strains could also be used to research cytophagy in cell model and mycobacterial colony and translocation in animal immunization as model indicator bacteria.